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1. 发明申请

WO1995011922A1 IN VITRO PEPTIDE AND ANTIBODY DISPLAY LIBRARIES 审中-公开
标题翻译：在体外肽和抗体显示图书馆
公开(公告)号：WO1995011922A1
公开(公告)日：1995-05-04
申请号：PCT/US1994012206
申请日：1994-10-25
申请人： AFFYMAX TECHNOLOGIES N.V. , MATTHEAKIS, Larry, C. , DOWER, William, J.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C07K16/00
CPC分类号： C12N15/1041 , C07K16/00 , C07K16/1285 , C07K2317/34
摘要： Improved methods and novel compositions for identifying peptides and single-chain antibodies that bind to predetermined receptors or epitopes. Such peptides and antibodies are indentified by improved and novel methods for affinity screening of polysomes displaying nascent peptides.
摘要翻译：用于鉴定结合预定受体或表位的肽和单链抗体的改进方法和新型组合物。通过用于亲和筛选显示新生肽的多核糖体的改进和新颖的方法来鉴定此类肽和抗体。

2. 发明申请

WO1992010588A1 SEQUENCING BY HYBRIDIZATION OF A TARGET NUCLEIC ACID TO A MATRIX OF DEFINED OLIGONUCLEOTIDES 审中-公开
标题翻译：通过将目标核酸杂交到定义的寡核苷酸的矩阵进行测序
公开(公告)号：WO1992010588A1
公开(公告)日：1992-06-25
申请号：PCT/US1991009226
申请日：1991-12-06
申请人： AFFYMAX TECHNOLOGIES N.V. , FODOR, Stephen, P., A. , SOLAS, Dennis, W. , DOWER, William, J.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C12Q01/68
CPC分类号： G03F7/00 , B01J19/0046 , B01J2219/00315 , B01J2219/00432 , B01J2219/00434 , B01J2219/00436 , B01J2219/00459 , B01J2219/00468 , B01J2219/00475 , B01J2219/005 , B01J2219/00527 , B01J2219/00529 , B01J2219/00531 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00648 , B01J2219/00659 , B01J2219/00689 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B82Y10/00 , B82Y30/00 , C07B2200/11 , C07H19/10 , C07H21/00 , C07K1/042 , C07K1/045 , C07K17/06 , C07K17/14 , C12Q1/6804 , C12Q1/6809 , C12Q1/6816 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6874 , C40B40/06 , C40B40/10 , C40B60/14 , G01N15/1475 , G03F7/265 , G03F7/38 , G11C13/0014 , G11C13/0019 , C12Q2565/513 , C12Q2565/507 , C12Q2545/101 , C12Q2525/185 , C12Q2565/514 , C12Q2563/173 , C12Q2563/119 , C12Q2563/149 , C12Q2525/151 , C12Q2525/179 , C12Q2525/204
摘要： The present invention provides methods and appartus for sequencing, fingerprinting and mapping biological polymers, particularly polynucleotides. The methods make use of a plurality of positionally distinct sequence specific recognition reagents, such as polynucleotides. The apparatus employs a substrate comprising positionally distinct sequence specific recognition reagents, such as polynucleotides, which are preferably localized at high densities. The methods and apparatus of the present invention can be used for determining the sequence of polynucleotides, mapping polynucleotides, and developing polynucleotide fingerprints. Polynucleotide fingerprints can be used for identifying individuals, tissue samples, pathological conditions, genetic diseases, infectious diseases, and other applications. Polynucleotide fingerprints can also be used for classification of bioligical samples, including taxonomy, and to characterize their sources. The invention also provides polynucleotide mapping, fingerprinting, and sequencing as valuable laboratory research tools for use in biological investigations.
摘要翻译：本发明提供了用于测序，指纹和映射生物聚合物，特别是多核苷酸的方法和应用。所述方法利用多种位置不同的序列特异性识别试剂，例如多核苷酸。该装置使用包含位置不同的序列特异性识别试剂（例如多核苷酸）的底物，其优选以高密度定位。本发明的方法和装置可以用于确定多核苷酸序列，映射多核苷酸和开发多核苷酸指纹。多核苷酸指纹可用于鉴定个体，组织样本，病理状况，遗传疾病，传染病和其他应用。多核苷酸指纹也可用于生物样本的分类，包括分类学，并对其来源进行表征。本发明还提供多核苷酸定位，指纹和测序作为用于生物学研究的有价值的实验室研究工具。

3. 发明申请

WO1992010587A1 SEQUENCING OF SURFACE IMMOBILIZED POLYMERS UTILIZING MICROFLUORESCENCE DETECTION 审中-公开
标题翻译：利用微量荧光检测的表面固定化聚合物的测序
公开(公告)号：WO1992010587A1
公开(公告)日：1992-06-25
申请号：PCT/US1991009217
申请日：1991-12-06
申请人： AFFYMAX TECHNOLOGIES N.V. , DOWER, William, J. , FODOR, Stephen, P., A.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C12Q01/68
CPC分类号： B01J19/0046 , B01J2219/00315 , B01J2219/00432 , B01J2219/00434 , B01J2219/00436 , B01J2219/00459 , B01J2219/00468 , B01J2219/00475 , B01J2219/005 , B01J2219/00527 , B01J2219/00529 , B01J2219/00531 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00648 , B01J2219/00659 , B01J2219/00689 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01L7/52 , B82Y10/00 , B82Y30/00 , C07B2200/11 , C07H19/10 , C07H21/00 , C07K1/042 , C07K1/045 , C07K17/06 , C07K17/14 , C12Q1/6804 , C12Q1/6809 , C12Q1/6816 , C12Q1/6823 , C12Q1/6837 , C12Q1/6869 , C12Q1/6874 , C12Q2535/101 , C40B40/06 , C40B40/10 , C40B60/14 , G01N15/1475 , G03F7/00 , G03F7/26 , G03F7/265 , G03F7/38 , G11C13/0014 , G11C13/0019 , C12Q2537/157 , C12Q2533/101 , C12Q2565/537 , C12Q2563/107 , C12Q2565/513 , C12Q2537/143 , C12Q2565/518
摘要： The present invention is based, in part, on the ability to perform a stepwise series of reactions which either extend or degrade a polymer by defined units, particularly by single monomer units. These stepwise reactions are preformed on polymers that are immobilized on a solid substrate in positionally distinct locations, preferably where such locations are packed on a single substrate at very high densities. The invention provides means for simultaneous parallel sequence analysis of a large number of biological polymer macromolecules, particularly polynucleotides. The invention provides apparatus and methods that may use fluorescent labels in repetitive chemical degradative steps to determine terminal monomers on solid phase immobilized polymers. Reagents which specifically recognize terminal monomers are used to label polymers at defined positions on a solid substrate.
摘要翻译：本发明部分地基于进行逐步一系列反应的能力，所述反应通过限定的单位延伸或降解聚合物，特别是单个单体单元。这些逐步反应在位于不同位置固定在固体基质上的聚合物上进行，优选在这样的位置以非常高的密度包装在单个基底上。本发明提供了用于同时并行序列分析大量生物聚合物大分子，特别是多核苷酸的方法。本发明提供了可以在重复的化学降解步骤中使用荧光标记物来测定固相固定化聚合物上的末端单体的装置和方法。专门识别末端单体的试剂用于在固体基质上的限定位置上标记聚合物。

4. 发明申请

WO1991017271A1 RECOMBINANT LIBRARY SCREENING METHODS 审中-公开
标题翻译：重组图书馆筛选方法
公开(公告)号：WO1991017271A1
公开(公告)日：1991-11-14
申请号：PCT/US1991002989
申请日：1991-05-01
申请人： AFFYMAX TECHNOLOGIES N.V. , DOWER, William, J. , CWIRLA, Steven, E.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C12Q01/70
CPC分类号： C40B40/02 , C07K16/00 , C07K16/005 , C12N15/1037
摘要： Nucleotide sequences encoding proteins of interest are isolated from DNA libraries using bacteriophage to link the protein to the sequence which encodes it. DNA libraries are prepared from cells encoding the protein of interest and inserted into or adjacent to a coat protein of a bacteriophage vector, or into a sequence encoding a protein which may be linked by means of a ligand to a phage coat protein. By employing affinity purification techniques the phage particles containing sequences encoding the desired protein may be selected and the desired nucleotide sequences obtained therefrom. Thus, for example, novel proteins such as monoclonal antibodies may be produced and conventional hybridoma technology avoided.
摘要翻译：使用噬菌体从DNA文库中分离编码感兴趣的蛋白质的核苷酸序列，以将蛋白质与编码它的序列连接。从编码感兴趣的蛋白质的细胞和插入到噬菌体载体的外壳蛋白中或邻近的蛋白质制备DNA文库，或者将其编码为可通过配体与噬菌体外壳蛋白连接的蛋白质的序列。通过使用亲和纯化技术，可以选择含有编码所需蛋白质的序列的噬菌体颗粒和从其获得的所需核苷酸序列。因此，例如，可以产生新的蛋白质，例如单克隆抗体，并避免常规的杂交瘤技术。

5. 发明申请

WO1995012608A1 SYNTHESIZING AND SCREENING MOLECULAR DIVERSITY 审中-公开
标题翻译：合成和筛选分子多样性
公开(公告)号：WO1995012608A1
公开(公告)日：1995-05-11
申请号：PCT/US1994012347
申请日：1994-11-02
申请人： AFFYMAX TECHNOLOGIES N.V. , SUGARMAN, Jeffrey, H. , RAVA, Richard, P. , KEDAR, Haim , DOWER, William, J. , BARRETT, Ronald, W. , GALLOP, Mark, A. , NEEDELS, Michael, C.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C07K01/04
CPC分类号： C07H21/00 , B01J19/0046 , B01J2219/00286 , B01J2219/00308 , B01J2219/00313 , B01J2219/00389 , B01J2219/00412 , B01J2219/00418 , B01J2219/00423 , B01J2219/00466 , B01J2219/00484 , B01J2219/00495 , B01J2219/005 , B01J2219/00545 , B01J2219/00547 , B01J2219/00554 , B01J2219/00572 , B01J2219/00574 , B01J2219/00585 , B01J2219/0059 , B01J2219/00592 , B01J2219/00596 , B01J2219/00689 , B01J2219/00695 , B01J2219/00698 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , C07K1/047 , C12Q1/6811 , C12Q1/6813 , C12Q1/6874 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14
摘要： A device and method for efficiently synthesizing diverse molecular products on substrates. A parent vessel (200) contains a suspension of substrates. The suspension is pressurized with argon and transferred to a plurality of reaction vessels (201-209) in one or more reaction vessel banks where monomer addition reactions take place. Optionally, the substrates may be tagged with a tag monomer. A vortexing motor (300) vortexes the contents of reaction vessels (201-209) during monomer addition reactions to enhance synthesis. After the desired monomer and/or tag monomer addition reaction, the suspension is pressurized with argon and transferred back to parent vessel (200) for mixing. Thereafter, the suspension may be pressurized with argon and reallocated among reaction vessels (201-209) for further synthesis.
摘要翻译：一种用于有效地合成基板上各种分子产物的装置和方法。母体容器（200）含有底物悬浮液。悬浮液用氩气加压并转移到一个或多个反应容器组中的多个反应容器（201-209），其中发生单体加成反应。任选地，可以用标签单体标记底物。涡旋马达（300）在单体加成反应期间涡旋反应容器（201-209）的内容物以增强合成。在所需的单体和/或标签单体加成反应之后，将悬浮液用氩气加压并转移回母体容器（200）以进行混合。此后，悬浮液可以用氩气加压并重新分配在反应容器（201-209）中进一步合成。

6. 发明申请

WO1994025043A1 PEPTIDES AND COMPOUNDS THAT BIND TO ELAM-1 审中-公开
标题翻译：绑定到ELAM-1的肽和化合物
公开(公告)号：WO1994025043A1
公开(公告)日：1994-11-10
申请号：PCT/US1993004281
申请日：1993-05-05
申请人： AFFYMAX TECHNOLOGIES N.V. , CWIRLA, Steven, E. , BARRETT, Ronald, W. , DOWER, William, J. , MARTENS, Christine, L.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： A61K37/00
CPC分类号： C07K7/08 , A61K38/00 , C07K14/70564
摘要： The peptide HITWDQLWNVMN and related peptides and peptidomimetics bind to endothelial leukocyte adhesion molecule 1 and block the binding of leukocytes to this important receptor and so can be used to ameliorate the detrimental effects of certain disease conditions.
摘要翻译：肽HITWDQLWNVMN和相关肽和肽模拟物结合内皮白细胞粘附分子1并阻断白细胞与这种重要受体的结合，因此可用于改善某些疾病状况的有害作用。

7. 发明申请

WO1993006121A1 METHOD OF SYNTHESIZING DIVERSE COLLECTIONS OF OLIGOMERS 审中-公开
标题翻译：合成多聚体多聚体的方法
公开(公告)号：WO1993006121A1
公开(公告)日：1993-04-01
申请号：PCT/US1992007815
申请日：1992-09-16
申请人： AFFYMAX TECHNOLOGIES N.V. , DOWER, William, J. , BARRETT, Ronald, W. , GALLOP, Mark, A. , NEEDELS, Michael, C.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C07H21/00
CPC分类号： C07K1/047 , B01J19/0046 , B01J19/0093 , B01J2219/00306 , B01J2219/00313 , B01J2219/00459 , B01J2219/00466 , B01J2219/005 , B01J2219/00502 , B01J2219/00536 , B01J2219/00542 , B01J2219/00545 , B01J2219/00547 , B01J2219/00554 , B01J2219/00563 , B01J2219/00565 , B01J2219/00572 , B01J2219/00574 , B01J2219/00576 , B01J2219/00585 , B01J2219/0059 , B01J2219/00592 , B01J2219/00596 , B01J2219/00689 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , C07B2200/11 , C07H19/10 , C07H19/14 , C07H21/00 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , Y10S435/81 , Y10T436/143333
摘要： A general stochastic method for synthesizing random oligomers can be used to synthesize compounds to screen for desired properties. The use of identification tags on the oligomers facilitates identification of oligomers with desired properties.
摘要翻译：可以使用用于合成无规低聚物的通用随机方法来合成化合物以筛选所需的性质。在寡聚体上使用鉴定标签便于鉴定具有所需性质的低聚物。

8. 发明申请

WO1991019818A1 PEPTIDE LIBRARY AND SCREENING SYSTEMS 审中-公开
标题翻译：肽库和筛选系统
公开(公告)号：WO1991019818A1
公开(公告)日：1991-12-26
申请号：PCT/US1991004384
申请日：1991-06-19
申请人： AFFYMAX TECHNOLOGIES N.V. , DOWER, William, J. , CWIRLA, Steven, E. , BARRETT, Ronald, W.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C12Q01/70
CPC分类号： C40B30/04 , C07K1/047 , C07K5/0806 , C07K5/0812 , C07K5/1008 , C07K5/1016 , C07K14/435 , C07K14/675 , C07K2319/00 , C12N15/1037 , C12N15/62 , C12N15/70 , C12Q1/70 , C40B40/02 , G01N33/6845 , G01N33/6878
摘要： Peptide which bind to selected receptors are identified by screening libraries which encode a random or controlled collection of amino acids. Peptides encoded by the libraries are expressed as fusion proteins of bacteriophage coat proteins, and bacteriophage are then screened against the receptors of interest. Peptides having a wide variety of uses, such as therapeutic or diagnostic reagents, may thus be identified without any prior information on the structure of the expected ligand or receptor.
摘要翻译：通过筛选编码随机或受控的氨基酸集合的文库鉴定与所选受体结合的肽。由文库编码的肽被表达为噬菌体外壳蛋白的融合蛋白，然后将噬菌体与感兴趣的受体进行筛选。因此可以鉴定具有多种用途的肽，例如治疗或诊断试剂，而没有关于预期配体或受体结构的任何先前信息。

9. 发明申请

WO1995018971A1 METHODS FOR THE SOLID PHASE SYNTHESIS OF GLYCOCONJUGATES 审中-公开
标题翻译：甘氨酸钠固相合成方法
公开(公告)号：WO1995018971A1
公开(公告)日：1995-07-13
申请号：PCT/US1995000484
申请日：1995-01-10
申请人： AFFYMAX TECHNOLOGIES N.V. , VETTER, Dirk , TUMELTY, David, A. , ANTONENKO, Valery
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： G01N33/543
CPC分类号： C07H13/04 , C07H3/04 , C07H3/06 , C07K1/04 , C07K1/047 , C07K9/00
摘要： An efficient and versatile method of forming N-linked glycoconjugates is described wherein a glycosyl acceptor, typically comprising an activated carboxyl group, is reacted with a glycosylating agent, typically a glycosylamine, in the presence of a coupling catalyst and optionally an exogenous base. Depending on the choice of reactive site, this method can be utilized to form N-linked glycoconjugates, in either a soluble or substrate-bound, linear or branched format.
摘要翻译：描述了形成N-连接的糖缀合物的有效和通用的方法，其中通常包含活化的羧基的糖基受体与糖基化剂（通常为糖胺）在偶联催化剂和任选的外源碱的存在下反应。根据反应位点的选择，该方法可用于以可溶或底物结合的线性或分支形式形成N-连接的糖缀合物。

10. 发明申请

WO1993010161A1 POLYMER REVERSAL ON SOLID SURFACES 审中-公开
标题翻译：聚合物反映固体表面
公开(公告)号：WO1993010161A1
公开(公告)日：1993-05-27
申请号：PCT/US1992009964
申请日：1992-11-19
申请人： AFFYMAX TECHNOLOGIES N.V. , HOLMES, Christopher, P.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C08F283/00
CPC分类号： B82Y30/00 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , C07B2200/11 , C07H21/00 , C07K1/04 , C07K1/042 , C40B40/06 , C40B40/10 , C40B60/14 , G03F7/00 , Y02P20/55
摘要： A method for cyclization and reversal of the polarity of polymers on a substrate. The method provides for the formation of a polymer on a substrate (2) with a tether molecule (4). Through unmasking of a protective group (PG2) a cyclic polymer (6) is formed. Through cleavage of an appropriate bond, a polarity reversed polymer (8) is formed. The method finds particular application in the formation of, for example, peptides and oligonucleotides.

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