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1. 发明申请

WO1998000567A1 IDENTIFICATION OF A TRANSFORMING FRAGMENT OF HERPES SIMPLEX TYPE 2 AND DETECTION THEREOF IN CLINICAL SPECIMENS 审中-公开
标题翻译：鉴别疱疹类型2的变形片段及其在临床样本中的检测
公开(公告)号：WO1998000567A1
公开(公告)日：1998-01-08
申请号：PCT/CA1997000470
申请日：1997-06-30
申请人： UNIVERSITÉ DE MONTRÉAL , KESSOUS, Allegria , COUTLEE, François , DiPAOLO, J., A.
发明人： UNIVERSITÉ DE MONTRÉAL
IPC分类号： C12Q01/70
CPC分类号： G01N33/56994 , C07K14/005 , C12N2710/16622 , C12Q1/6886 , C12Q1/705 , G01N2333/035
摘要： The present invention relates to oligonucleotide probes derived from HSV-2, capable of selectively hybridizing thereto and to a subsequence of HSV-2 Bgl II N from which the oligonucleotide probes were derived. Further, the invention relates to an optimized assay of nucleic acid amplification permitting the sensitive and specific detection in clinical speciments of HSV-2 as well as a specific typing of the HSV in the sample. The present invention further relates to kits for the detection and typing of the HSV in a sample. In addition, the invention provides the nucleic acid and amino acid sequence of a subsequence of HSV-2 Bgl II N having transforming activity. Further, the invention teaches diagnostic and therapeutic methods for genital cancer comprising the use of these sequences or ligands directed thereto.
摘要翻译：本发明涉及能够选择性杂交的HSV-2的寡核苷酸探针和衍生出寡核苷酸探针的HSV-2 Bgl II N的亚序列。此外，本发明涉及核酸扩增的优化测定，其允许在HSV-2的临床特征中的敏感和特异性检测以及样品中HSV的特异性分型。本发明还涉及用于在样品中检测和分型HSV的试剂盒。此外，本发明提供具有转化活性的HSV-2 Bgl II N的亚序列的核酸和氨基酸序列。此外，本发明教导了用于生殖器癌症的诊断和治疗方法，包括使用这些序列或指向其的配体。

2. 发明申请

WO1997022722A1 GENETIC SUPPRESSOR ELEMENTS AGAINST HUMAN IMMUNODEFICIENCY VIRUS 审中-公开
标题翻译：遗传抑制因子对人体免疫缺陷病毒的影响
公开(公告)号：WO1997022722A1
公开(公告)日：1997-06-26
申请号：PCT/US1996020435
申请日：1996-12-20
申请人： INGENEX, INC.
发明人： INGENEX, INC. , HOLZMAYER, Tanya, A. , DUNN, Stephen, J. , PARK, Suk, W. , DAYN, Andrew
IPC分类号： C12Q01/70
CPC分类号： C12N15/1132 , C07K14/005 , C12N2310/111 , C12N2740/16322
摘要： The present invention relates to genetic elements that suppress the activities of the human immunodeficiency virus (HIV). In particular, the invention relates to polynucleotides isolated from the HIV-1 genome, methods for isolating and identifying such polynucleotides, and methods for using them for the protection of human cells against HIV infection and/or replication.
摘要翻译：本发明涉及抑制人类免疫缺陷病毒（HIV）活性的遗传因素。特别地，本发明涉及从HIV-1基因组分离的多核苷酸，用于分离和鉴定此类多核苷酸的方法，以及用于保护人类细胞免受HIV感染和/或复制的方法。

3. 发明申请

WO1997001648A1 A NOVEL HUMAN PROTEIN CRITICAL FOR HIV REPLICATION 审中-公开
标题翻译：一个新的人类蛋白质关键的HIV复制
公开(公告)号：WO1997001648A1
公开(公告)日：1997-01-16
申请号：PCT/US1996011078
申请日：1996-06-27
申请人： UNIVERSITY OF MASSACHUSETTS MEDICAL CENTER
发明人： UNIVERSITY OF MASSACHUSETTS MEDICAL CENTER , FRITZ, Christian, C. , GREEN, Michael, R.
IPC分类号： C12Q01/70
CPC分类号： C12N9/16 , C07K14/47 , C07K14/4722 , C07K16/18
摘要： The present invention provides a human cellular polypeptide, termed hRIP, that is necessary for HIV replication. The polypeptide has a molecular mass of about 59 kDa, having the sequence set forth in the figure (SEQ ID NO:1). hRIP is the cellular cofactor required to mediate the Rev response. hRIP binds HIV Rev protein and is essential for Rev activity. The invention also includes nucleic acid sequences encoding hRIP, as well as DNA vectors and transformed cells suitable for recombinant expression of this polypeptide.
摘要翻译：本发明提供了HIV复制所必需的人细胞多肽，称为hRIP。该多肽具有约59kDa的分子量，其具有图中所示的序列（SEQ ID NO：1）。 hRIP是调节Rev反应所需的细胞辅因子。 hRIP结合HIV Rev蛋白，对于Rev活性至关重要。本发明还包括编码hRIP的核酸序列，以及适合于重组表达该多肽的DNA载体和转化细胞。

4. 发明申请

WO1996039542A1 CULTURED CELL LINE THAT INDUCIBLY EXPRESSES THE HEPATITIS B VIRUS GENOME, AND USES THEREOF FOR SCREENING ANTIVIRAL SUBSTANCES 审中-公开
标题翻译：培养细胞系，其诱导表达乙型肝炎病毒基因组及其用于筛选抗病毒物质的用途
公开(公告)号：WO1996039542A1
公开(公告)日：1996-12-12
申请号：PCT/US1996008772
申请日：1996-06-05
申请人： AVID THERAPEUTICS
发明人： AVID THERAPEUTICS , KING, Robert, W. , BARKER, Christopher, S. , SEEGER, Christopher
IPC分类号： C12Q01/70
CPC分类号： G01N33/502 , C07K14/005 , C12N2730/10122 , G01N33/5008 , G01N33/5067 , G01N2333/02
摘要： Novel cell lines and methods are provided for inducibly expressing the Hepatitis B virus (HBV) genome within cultured cells. Cells are stably transformed with an HBV genome under control of an operator/promoter target sequence which can activate expression of the HBV genome when bound by a cognate transactivator protein. The cell lines are also stably transformed with a gene that encodes and expresses a suitable transactivator protein. Cells may be maintained under non-inducing conditions whereby the transactivator protein is unable to interact with the operator/promoter target sequence, so the HBV genome is not expressed. Under appropriate inducing conditions, the transactivator protein is enabled to interact with the operator/promoter target sequence, thereby activating expression of the HBV genome. The inducible system provides an effective and rapid cell-based assay to screen compounds for anti-hepadnaviral activity.
摘要翻译：提供新的细胞系和方法用于在培养细胞内诱导性表达乙型肝炎病毒（HBV）基因组。细胞在用基因启动子靶序列控制下用HBV基因组稳定转化，当与同源反式激活蛋白结合时可以激活HBV基因组的表达。细胞系也用编码并表达合适的反式激活蛋白的基因稳定转化。细胞可以保持在非诱导条件下，由此反式激活因子蛋白不能与操纵子/启动子靶序列相互作用，因此HBV基因组不表达。在适当的诱导条件下，反式激活蛋白能够与操纵子/启动子靶序列相互作用，从而激活HBV基因组的表达。诱导系统提供有效和快速的基于细胞的测定以筛选化合物用于抗肝炎病毒活性。

5. 发明申请

WO1996030547A1 METHOD FOR DIAGNOSIS OF EPSTEIN-BARR VIRUS ASSOCIATED DISEASE 审中-公开
标题翻译：用于诊断EPSTEIN-BARR病毒相关疾病的方法
公开(公告)号：WO1996030547A1
公开(公告)日：1996-10-03
申请号：PCT/US1996004377
申请日：1996-03-29
申请人： ORTHO DIAGNOSTIC SYSTEMS, INC.
发明人： ORTHO DIAGNOSTIC SYSTEMS, INC. , SMITH, Richard, S. , PARKS, Elliot, D.
IPC分类号： C12Q01/70
CPC分类号： G01N33/56994 , A61K2039/505 , C07K14/005 , C12N2710/16222
摘要： A novel assay utilizing Epstein-Barr virus (EBV) specific peptides is disclosed. The assay is particularly useful for detecting early antigen antibodies in a blood sample from an individual having an EBV-associated disease, the disease preferably being infectious mononucleosis.
摘要翻译：公开了一种利用爱泼斯坦 - 巴尔病毒（EBV）特异性肽的新方法。该测定法特别可用于检测来自具有EBV相关疾病的个体的血液样品中的早期抗原抗体，该疾病优选为感染性单核细胞增多症。

6. 发明申请

WO1996024693A1 METHODS FOR TESTING OF THERAPEUTIC COMPOUNDS 审中-公开
标题翻译：治疗化合物检测方法
公开(公告)号：WO1996024693A1
公开(公告)日：1996-08-15
申请号：PCT/SE1996000078
申请日：1996-01-25
申请人： ASTRA AKTIEBOLAG , KRISTOFFERSON, Ann
发明人： ASTRA AKTIEBOLAG
IPC分类号： C12Q01/70
CPC分类号： A61K49/0006
摘要： The present invention is related to a method for determining the efficacy of a chemical compound for treatment of recurrent herpes virus infection, said method comprising: (a) inoculating herpes virus at a primary skin site of a non-human mammal to cause zosteriform spread of the virus through nerve cells to cause lesions at a secondary (distal) site of infection; (b) before the appearance of skin lesions at the secondary site caused by the virus, giving adoptive transfer of immunity (ATI) to the said non-human mammal, so as to provide immunity mimicking the immunological reactivity seen in a recurrent herpes virus infection in a previously infected immune host; (c) administering a chemical compound to be tested to the said non-human mammal; and (d) determining the ability of the said chemical compound to reduce the occurrence and/or severity of herpes virus-induced skin lesions at the secondary site of infection.
摘要翻译：本发明涉及一种用于确定化合物治疗复发性疱疹病毒感染的功效的方法，所述方法包括：（a）在非人哺乳动物的原皮肤部位接种疱疹病毒以引起疱疹病毒感染病毒通过神经细胞在继发（远端）感染部位引起病变; （b）在由病毒引起的次级部位出现皮肤损伤之前，对所述非人哺乳动物进行过继免疫转移（ATI），以提供模仿复发性疱疹病毒感染中所见的免疫反应的免疫力在先前感染的免疫宿主中; （c）向所述非人哺乳动物施用待测化合物; 和（d）确定所述化合物减少感染次要部位的疱疹病毒诱导的皮肤损伤的发生和/或严重性的能力。

7. 发明申请

WO1996021043A1 USING LAMBDOID BACTERIOPHAGES LINKED BY OLIGONUCLEOTIDES TO SOLID SUPPORT 审中-公开
标题翻译：使用由寡核苷酸连接的LABBDOID BACTERIOPAGES结合实际支持
公开(公告)号：WO1996021043A1
公开(公告)日：1996-07-11
申请号：PCT/US1996000108
申请日：1996-01-02
申请人： SYMBIOTECH, INC.
发明人： SYMBIOTECH, INC. , RAY, Bryan, L. , LIN, Edmund, C., C.
IPC分类号： C12Q01/70
CPC分类号： C12Q1/706 , A61K47/6901 , C07K14/005 , C12N7/00 , C12N2795/10321 , C12N2795/10322 , C12N2795/10345 , C12N2795/10362 , C12Q1/6823 , C12Q1/70 , C12Q1/707 , C12Q2521/301
摘要： The present invention provides compositions, methods and kits for detection and quantitation of pathogenic organisms. The composition of the invention is an oligonucleotide probe comprising a bacteriophage covalently linked to one site on an oligonucleotide probe complementary to a conserved region of a pathogenic organism. At a second site, the oligonucleotide probe is linked to a matrix. The oligonucleotide probe contains a region complementary to one strand of a restriction endonuclease recognition site or an oligoribonucleotide moiety. The number of pathogenic organisms present in a biological fluid sample may be quantitated in accordance with the method of the invention by combining the composition of the invention with the sample, allowing hybridization to occur. Hybridization generates the intact restriction endonuclease site or a DNA-RNA hybrid, and by adding the appropriate restriction endonuclease or a nucleolytic enzyme capable of cleaving DNA-RNA hybrids, bacteriophage will be released for measurement. The kit of the invention provides components which allow the method of the invention to be performed.
摘要翻译：本发明提供了用于病原生物体的检测和定量的组合物，方法和试剂盒。本发明的组合物是一种寡核苷酸探针，其包含与与致病生物体的保守区域互补的寡核苷酸探针上的一个位点共价连接的噬菌体。在第二个位点，寡核苷酸探针与基质连接。寡核苷酸探针含有与限制性内切核酸酶识别位点的一条链或寡核糖核苷酸部分互补的区域。存在于生物流体样品中的致病生物体的数量可以根据本发明的方法通过将本发明的组合物与样品组合进行定量，从而进行杂交。杂交产生完整的限制性内切酶位点或DNA-RNA杂交体，通过加入适当的限制性内切核酸酶或能够切割DNA-RNA杂交体的核解质酶，噬菌体将被释放用于测量。本发明的试剂盒提供允许本发明方法进行的组分。

8. 发明申请

WO1996013616A1 PEPTIDES FOR DETECTION OF HEPATITIS C ANTIBODIES 审中-公开
标题翻译：用于检测丙型肝炎抗体的肽
公开(公告)号：WO1996013616A1
公开(公告)日：1996-05-09
申请号：PCT/US1995013660
申请日：1995-10-23
申请人： UNITED BIOMEDICAL, INC.
发明人： UNITED BIOMEDICAL, INC. , WANG, Chang, Yi , HOSEIN, Barbara, H.
IPC分类号： C12Q01/70
CPC分类号： C07K14/005 , A61K38/00 , C12N2770/24222 , G01N33/5767 , Y10S436/82
摘要： The present invention relates to novel linear and branched peptides specific for the diagnosis and prevention of hepatitis C virus (HCV) infection. More particularly, the present invention is directed to linear and branched synthetic peptides containing at least one antigenic site which is effective for detecting HCV-associated antibodies in patients using immunoassay techniques. In some cases, these peptides are also library peptides.
摘要翻译：本发明涉及特异于丙型肝炎病毒（HCV）感染的诊断和预防的新型线性和支链肽。更具体地说，本发明涉及含有至少一个抗原位点的线性和支链合成肽，该抗原位点在使用免疫测定技术的患者中检测HCV相关抗体是有效的。在某些情况下，这些肽也是文库肽。

9. 发明申请

WO1996009411A1 ANTIGEN BINDING PEPTIDES (ABTIDES) FROM PEPTIDE LIBRARIES 审中-公开
标题翻译：来自肽库的抗原结合肽（ABTIDES）
公开(公告)号：WO1996009411A1
公开(公告)日：1996-03-28
申请号：PCT/US1995011934
申请日：1995-09-20
申请人： CYTOGEN CORPORATION
发明人： CYTOGEN CORPORATION , ALVAREZ, Vernon, L.
IPC分类号： C12Q01/70
CPC分类号： C40B30/04 , A61K38/00 , C07K1/047 , C07K14/001 , C07K14/4727 , C07K2319/00 , C12N15/1037 , C40B40/02 , G01N33/6845 , G01N33/6854
摘要： Abtides are provided. Abtides are peptides identified by a two-step process of screening random peptide libraries. In the first step, the target ligand is an antibody or receptor (or derivative thereof). The peptides identified in the first screening step are used as target ligands in the second screening step. The peptides identified in the second screening step are abtides. Abtides possess binding specificities that are similar to the binding specificities of the antibodies or receptors that are used in the first screening step. Abtides may be used in place of antibodies in many assays or therapeutic applications. Abtides binding to polymorphic epithelial mucin (PEM) are provided. Also provided are methods of obtaining abtides as well as diagnostic and therapeutic compounds containing abtides.
摘要翻译：提供Abtides。 Abtides是通过筛选随机肽文库的两步方法鉴定的肽。在第一步中，靶配体是抗体或受体（或其衍生物）。将第一筛选步骤中鉴定的肽用作第二筛选步骤中的靶配体。在第二筛选步骤中鉴定的肽是抗生素。 Abtides具有与第一筛选步骤中使用的抗体或受体的结合特异性相似的结合特异性。在许多测定或治疗应用中，可以使用Abtides代替抗体。提供了与多形性上皮粘蛋白（PEM）的结合。还提供了获得抗坏血酸的方法以及含有abtides的诊断和治疗化合物。

10. 发明申请

WO1995034685A1 METHOD OF DETECTING CELLULAR MATERIAL 审中-公开
标题翻译：检测细胞材料的方法
公开(公告)号：WO1995034685A1
公开(公告)日：1995-12-21
申请号：PCT/US1995007466
申请日：1995-06-13
申请人： GENZYME CORPORATION
发明人： GENZYME CORPORATION , MAHR, Anna, M. , BOWE, Ann, E. , RUFF-ROBERTS, Alyson, L. , KLINGER, Katherine, W.
IPC分类号： C12Q01/70
CPC分类号： C12Q1/6806 , C12Q1/6841
摘要： The method of the present invention relates to a rapid procedure for detecting DNA in a cell, while preserving the morphology of the nucleus for analysis. The method comprises depositing a cell onto a polymeric membrane filter wherein the DNA contained in the cell is retained on the polymeric membrane filter and is available for binding with a fluorescently labeled nucleic acid probe, incubating the polymeric membrane filter with the labeled nucleic acid probe, and detecting the labeled nucleic acid probe wherein detection of the nucleic acid probe is indicative of the presence of the DNA. There are no separate permeabilization steps needed to permit the probes to enter the cell and hybridize to the DNA. The method is simple and quick and is applicable to the sample volumes found in clinical laboratories. The method of the present invention allows for analysis with single copy sequence probes of cells sorted onto, filtered onto, grown on or settled onto polymeric membrane filters. In addition, the method allows for the hybridization of probes containing single copy or repetitive DNA sequences to metaphase chromosomes as well as to interphase nuclei on membrane filters.
摘要翻译：本发明的方法涉及用于检测细胞中DNA的快速方法，同时保留用于分析的细胞核的形态。该方法包括将细胞沉积在聚合物膜过滤器上，其中包含在细胞中的DNA保留在聚合物膜过滤器上，并且可用于与荧光标记的核酸探针结合，将聚合物膜过滤器与标记的核酸探针，并检测标记的核酸探针，其中核酸探针的检测指示DNA的存在。不需要单独的透化步骤来允许探针进入细胞并与DNA杂交。该方法简便快捷，适用于临床实验室的样品量。本发明的方法允许用分选到，过滤到，生长在或沉积在聚合物膜过滤器上的细胞的单拷贝序列探针进行分析。此外，该方法允许将含有单拷贝或重复DNA序列的探针杂交到中期染色体以及膜过滤器上的间期核。

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