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1. 发明申请

WO2016119448A3 ARTIFICIAL EXOGENOUS REFERENCE MOLECULE FOR COMPARING TYPES AND NATURAL ABUNDANCE BETWEEN MICROORGANISMS OF DIFFERENT SPECIES AND GENERA 审中-公开
标题翻译：人造异常参考分子，用于比较不同种类和GENERA微生物之间的类型和自然遗传
公开(公告)号：WO2016119448A3
公开(公告)日：2017-03-30
申请号：PCT/CN2015087624
申请日：2015-08-20
申请人： THE FIRST HOSPITAL OF CHINA MEDICIAL UNIV
发明人： GAO XINGHUA , QI RUIQUN , HONG YUXIAO
IPC分类号： C12N15/11 , C12Q1/68
CPC分类号： C12Q1/6888 , C12Q1/686 , C12Q1/689 , C12Q2525/161 , C12Q2537/143 , C12Q2545/101
摘要： Disclosed is an artificial exogenous reference molecule for comparing the types and natural abundance between microorganisms of different species and genera, comprising: several species test zones and several linker sequence zones, the species test zones and linker sequence zones being arranged alternatingly; the species test zones number at least 2 and are for testing different species and genera; the linker sequence zones are random sequences different from the species sequences to be tested. A species test zone comprises a primer sequence zone and a specificity identification zone, the length of the sequence in the specificity identification zone emulating the length of the characteristic test sequence of the corresponding species to be tested, the sequence in the specificity identification zone being not homologous with the genome DNA sequence in the species to be tested and having obvious specificity differences. Also disclosed is a method for using the artificial exogenous reference molecule. The artificial exogenous reference molecule provided in the present invention may simultaneously contain the consensus primer sequence zone of two or more than two different microorganisms, and contains human-identifiable specificity sequences for comparing the types and natural abundance between microorganisms of different species and genera.
摘要翻译：公开了一种用于比较不同物种和属的微生物之间的类型和天然丰度的人造外源参考分子，包括：几个物种测试区和几个接头序列区，物种测试区和接头序列区交替排列; 物种试验区至少2个，用于测试不同种类和属; 接头序列区是与要测试的物种序列不同的随机序列。物种测试区包括引物序列区和特异性鉴定区，特异性鉴定区中序列的长度模拟相应待测物种的特征测试序列的长度，特异性鉴定区中的序列不是与待测物种中的基因组DNA序列同源，具有明显的特异性差异。还公开了使用人造外源参考分子的方法。本发明提供的人造外源参考分子可以同时含有两种或多于两种不同微生物的共有引物序列区，并且含有用于比较不同物种和属的微生物之间的类型和天然丰度的人可鉴定的特异性序列。

2. 发明申请

WO2016153355A2 NOVEL ISOLATION AND AMPLIFICATION PROCESS CONTROL 审中-公开
标题翻译：新型隔离和放大过程控制
公开(公告)号：WO2016153355A2
公开(公告)日：2016-09-29
申请号：PCT/NL2016/050216
申请日：2016-03-29
申请人： MICROBIOME LIMITED
发明人： SAVELKOUL, Paul Hendrik Maria
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q1/6846 , C12Q2600/166 , C12Q2545/101 , C12Q2549/119
摘要： The invention relates to a positive control nucleic acid molecule comprising first and second primer hybridization sequences derived from a first microorganism which flank an internal nucleic acid sequence that is not naturally present in the first microorganism. The invention further relates to a positive process control microorganism comprising said positive control nucleic acid molecule, to a receptacle comprising said positive control microorganism, and to methods for determining whether a first microorganism is present or absent in a test sample, comprising contacting the test sample with the positive process control microorganism and generating amplification products using first and second primers that hybridize to the first and second primer hybridization sequences.
摘要翻译：本发明涉及阳性对照核酸分子，其包含衍生自第一微生物的第一和第二引物杂交序列，所述第一和第二引物杂交序列位于第一微生物中不天然存在的内部核酸序列。本发明还涉及包含所述阳性对照核酸分子的阳性过程控制微生物，包含所述阳性对照微生物的容器，以及用于确定测试样品中是否存在第一微生物的方法，包括使所述测试样品与阳性过程控制微生物和使用与第一和第二引物杂交序列杂交的第一和第二引物产生扩增产物。

3. 发明申请

WO2016138105A2 ASSAYS TO DETERMINE DNA METHYLATION AND DNA METHYLATION MARKERS OF CANCER 审中-公开
标题翻译：测定癌症的DNA甲基化和DNA甲基化标记
公开(公告)号：WO2016138105A2
公开(公告)日：2016-09-01
申请号：PCT/US2016/019310
申请日：2016-02-24
申请人： ZYMO RESEARCH CORPORATION
发明人： GUO, Wei , PIATTI, Paolo , YANG, Xiaojing , JIA, Xi-Yu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q2521/331 , C12Q2523/125 , C12Q2537/143 , C12Q2545/101 , C12Q2561/113 , C12Q2563/107 , C12Q2561/101
摘要： Methods are provided for determining a genomic methylation profile in a DNA sample. In certain aspects, the methods can be used to determine if a subject has, or is at risk for developing, a bladder cancer or other cancers of the urinary tract. Methods for treatment of such subjects are likewise provided.
摘要翻译：提供了用于确定DNA样品中的基因组甲基化谱的方法。在某些方面，该方法可用于确定受试者是否具有或有发展为膀胱癌或其它尿道癌症的风险。同样提供了治疗这些受试者的方法。

4. 发明申请

WO2015165779A3 SMALL NCRNAS AS BIOMARKERS 审中-公开
标题翻译：小NCRNAS作为生物标记
公开(公告)号：WO2015165779A3
公开(公告)日：2016-01-28
申请号：PCT/EP2015058614
申请日：2015-04-21
申请人： STICHTING VU VUMC , UNIV GRANADA
发明人： PEGTEL DIRK MICHIEL , KOPPERS-LALIC DANIJELA , WURDINGER TOM , BIJNSDORP IRENE , HACKENBERG MICHAEL
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , A61K31/166 , A61K31/475 , A61K31/573 , A61K31/675 , A61K31/704 , A61K31/7048 , A61K35/545 , A61K38/14 , C12Q1/6809 , C12Q2600/118 , C12Q2600/178 , C12Q2525/207 , C12Q2535/122 , C12Q2537/165 , C12Q2545/101
摘要： The present disclosure provides small ncRNAs as biomarkers for classifying the health status of an individual. The disclosure also provides screening methods for identifying ncRNA biomarkers.
摘要翻译：本公开提供小的ncRNA作为用于分类个体的健康状况的生物标志物。本公开还提供用于鉴定ncRNA生物标志物的筛选方法。

5. 发明申请

WO2015185551A1 SCREENING ASSAY FOR IDENTIFYING miRNA-SPECIFIC DRUG COMPOUNDS 审中-公开
标题翻译：用于鉴定miRNA特异性药物化合物的筛选测定
公开(公告)号：WO2015185551A1
公开(公告)日：2015-12-10
申请号：PCT/EP2015/062258
申请日：2015-06-02
申请人： ETH ZURICH
发明人： BENENSON, Yaakov , HÄFLIGER, Benjamin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6897 , C12Q2525/207 , C12Q2545/101
摘要： The present invention relates to a method for identifying miRNA (microRNA)- modulating compounds by contacting a compound of interest with a cell comprising (i) a "miRNA-specific module" that reports specific miRNA target sequence binding by means of a first reporter product; (ii) a "non-specific RNAi module" that reports altered expression of one or more endogenous miRNAs with baseline expression levels in the cell ("non-specific miRNA markers") by means of a second reporter product; and optionally (iii) a "gene expression module" that reports global changes in gene expression; and subsequent detection of changes in reporter product expression. The advantage of this method is that non-specific miRNA- and/or RNAi-effects of the compound of interest are detected and false positive results due to the structural similarity of different miRNAs and the commonly shared maturation pathway for most miRNAs are substantially reduced over conventional miRNA screening assays.
摘要翻译：本发明涉及通过使感兴趣的化合物与细胞接触来鉴定miRNA（微小RNA）调节化合物的方法，所述细胞包括（i）通过第一报道产物报告特异性miRNA靶序列结合的“miRNA特异性模块” ; （ii）通过第二报道产物报告在细胞中具有基线表达水平（“非特异性miRNA标志物”）的一种或多种内源性miRNA的表达改变的“非特异性RNAi模块” 和（iii）报告全基因表达变化的“基因表达模块”; 并随后检测报告产物表达的变化。该方法的优点是检测到目标化合物的非特异性miRNA和/或RNAi效应，并且由于不同miRNA的结构相似性和大多数miRNA的共同共享成熟途径而导致假阳性结果大大降低常规miRNA筛选试验。

6. 发明申请

WO2015159122A1 NUCLEIC ACID BASED SENSOR AND METHODS THEREOF 审中-公开
标题翻译：基于核酸的传感器及其方法
公开(公告)号：WO2015159122A1
公开(公告)日：2015-10-22
申请号：PCT/IB2014/060736
申请日：2014-04-15
申请人： NATIONAL CENTRE FOR BIOLOGICAL SCIENCES
发明人： KRISHNAN, Yamuna , SAHA, Sonali , PRAKASH, Ved
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6825 , C12Q2525/107 , C12Q2545/101 , C12Q2565/101 , C12Q2565/519 , C12Q2565/607
摘要： The present disclosure relates to a nucleic acid based sensor, comprising a sensing 5 module, a normalizing module and a targeting module. It also relates to a method of obtaining and targeting the sensor and its use to identify and optionally quantify a target in a specific cellular microenvironment.
摘要翻译：本公开涉及基于核酸的传感器，其包括感测5模块，归一化模块和目标模块。它还涉及获得和靶向传感器的方法及其用于鉴定和任选地定量特定细胞微环境中的靶标的方法。

7. 发明申请

WO2015143385A1 MULTI-COPY REFERENCE ASSAY 审中-公开
标题翻译：多重参考测量
公开(公告)号：WO2015143385A1
公开(公告)日：2015-09-24
申请号：PCT/US2015/021853
申请日：2015-03-20
申请人： LIFE TECHNOLOGIES CORPORATION
发明人： MERRILL, David , BRZOSKA, Pius , LI, Zheng , LIN, Wendy , LEE, Wing , WONG, Mandi
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6851 , C12Q1/6858 , C12Q2600/156 , C12Q2600/158 , C12Q2600/166 , C12Q2537/16 , C12Q2543/10 , C12Q2545/101 , C12Q2547/107 , C12Q2561/101
摘要： A method, comprising amplifying a nucleic acid sequence of interest in a sample comprising genomic DNA of a subject; amplifying a reference nucleic acid sequence in the sample; quantifying the amplified sequence of interest relative to the amplified reference sequence; and determining a copy number of the sequence of interest from the relative quantified amplified sequence of interest. The reference sequence may have at least 80% sequence identity to at least one of SEQ ID NO:1-38, such as SEQ ID NO:1-13. Also disclosed are kits and compositions, each comprising a first probe which specifically hybridizes to at least a portion of at least one reference sequence. Also disclosed is a system configured to perform the above method.
摘要翻译：一种方法，包括在包含受试者的基因组DNA的样品中扩增目的核酸序列; 扩增样品中的参考核酸序列; 相对于扩增的参考序列量化扩增的感兴趣的序列; 以及从所述相关量化的放大感兴趣序列确定感兴趣序列的拷贝数。参考序列可以与SEQ ID NO：1-38中的至少一个具有至少80％的序列同一性，例如SEQ ID NO：1-13。还公开了试剂盒和组合物，每种试剂盒和组合物包含与至少一个参考序列的至少一部分特异性杂交的第一探针。还公开了配置为执行上述方法的系统。

8. 发明申请

WO2015083004A1 METHOD FOR EVALUATING MINORITY VARIANTS IN A SAMPLE 审中-公开
标题翻译：在样本中评估少数变量的方法
公开(公告)号：WO2015083004A1
公开(公告)日：2015-06-11
申请号：PCT/IB2014/003082
申请日：2014-11-26
申请人： POPULATION GENETICS TECHNOLOGIES LTD.
发明人： OSBORNE, Robert , MUSGRAVE-BROWN, Esther
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , G06F19/22 , C12Q2535/122 , C12Q2545/101
摘要： A method of evaluating a sequence variation in a sample is provided. In some embodiments, the method may involve: amplifying a nucleic acid product from an initial sample; fragmenting an amount of the nucleic acid product to produce fragments; attaching an adaptor to each end of the fragments to produce adaptor- tagged fragments; sampling no more than 10% of the tagged fragments and amplifying them; sequencing at least some of the copies of the fragments to produce a plurality of sequence reads; grouping sequence reads for copies of fragments that have the same fragmentation breakpoints; deriving a consensus sequence for each of the read groups; and aligning the consensus sequences with a reference sequence.
摘要翻译：提供了评估样品中序列变异的方法。在一些实施方案中，所述方法可以包括：从初始样品扩增核酸产物; 破碎一定量的核酸产物以产生片段; 将适配器连接到片段的每一端以产生适应性标记的片段; 取代不超过10％的标记片段并扩增它们; 对片段的至少一些拷贝进行测序以产生多个序列读数; 分组序列读取具有相同分段断点的片段的副本; 导出每个读取组的共有序列; 并将共有序列与参考序列对齐。

9. 发明申请

WO2015067796A1 GENETIC ANALYSIS METHOD 审中-公开
标题翻译：遗传分析方法
公开(公告)号：WO2015067796A1
公开(公告)日：2015-05-14
申请号：PCT/EP2014/074155
申请日：2014-11-10
申请人： CARTAGENIA N.V.
发明人： DEVOGELAERE, Benoit , VERRELST, Herman
IPC分类号： C12Q1/68
CPC分类号： G06F19/18 , C12Q1/6869 , C12Q1/6874 , G06F19/24 , C12Q2521/301 , C12Q2525/191 , C12Q2535/122 , C12Q2545/101
摘要： A method of target DNA genome analysis is provided. The method comprises the steps of : - obtaining non-overlapping segments of target DNA stretches with segment boundaries defined by the presence of particular restriction enzyme recognition sites, whereby the assembly of said non-overlapping segments compose a reduced representation library of said target DNA genome; - obtaining for said segments, raw metrics from a sequencing process applied on said reduced representation library; - clustering non-overlapping, nearby segments with similar raw metrics to provide master segments; - providing metrics describing the master segments, - making a final discrete DNA call based on the master segments and its metrics.
摘要翻译：提供了靶DNA基因组分析的方法。该方法包括以下步骤： - 获得具有由特定限制酶识别位点的存在限定的区段边界的靶DNA延伸的非重叠区段，由此所述非重叠区段的组装构成所述靶DNA基因组的缩小表示文库 ; - 对所述段获得应用于所述简化表示库的排序过程的原始度量; - 使用类似的原始度量来聚集不重叠的附近细分，以提供主分段; - 提供描述主节点的度量， - 根据主节点及其度量进行最终的离散DNA呼叫。

10. 发明申请

WO2014152937A1 NUCLEIC ACID CONTROL PANELS 审中-公开
标题翻译：核酸控制面板
公开(公告)号：WO2014152937A1
公开(公告)日：2014-09-25
申请号：PCT/US2014/028324
申请日：2014-03-14
申请人： IBIS BIOSCIENCES, INC. , MARBLE, Herbert A.
发明人： MARBLE, Herbert A.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6869 , C12Q1/6876 , C12Q2600/166 , C12Q2535/122 , C12Q2545/107 , C12Q2547/101 , C12Q2531/113 , C12Q2537/143 , C12Q2545/101
摘要： Provided herein is technology relating to detecting nucleic acids in a sample and particularly, but not exclusively, to systems and methods related to panels that are used to evaluate sequencing efficacy. Mutations/variations in the human genome are involved in many diseases, ranging from monogenetic to multifactorial diseases, and acquired diseases such as cancer. Even the susceptibility to infectious diseases, and the response to pharmaceutical drugs, is affected by the composition of an individual's genome.
摘要翻译：本文提供了与检测样品中的核酸有关的技术，特别地但不排他地涉及与用于评估测序功效的面板有关的系统和方法。人类基因组中的突变/变异涉及许多疾病，从单因素到多因素疾病以及获得性疾病如癌症。即使对传染病的敏感性和对药物的反应也受到个体基因组成分的影响。

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