专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2019135091A1 METHOD FOR SELECTING POLYNUCLEOTIDES BASED ON ENZYME INTERACTION DURATION 审中-公开
公开(公告)号：WO2019135091A1
公开(公告)日：2019-07-11
申请号：PCT/GB2019/050029
申请日：2019-01-07
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： HERON, Andrew John , BOWEN, Rebecca Victoria
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , C12Q2521/513 , C12Q2525/191 , C12Q2525/204 , C12Q2527/113 , C12Q2565/631
摘要： A method for selecting polynucleotides, the method comprising: allowing a nucleic acid handling enzyme to move along multiple polynucleotides in a sample for a defined time period, wherein the enzyme is loaded onto each of the multiple polynucleotides and wherein one or more molecule of the enzyme moves along each of the multiple polynucleotides; and selecting polynucleotides based on whether or not the enzyme reaches the end of and/or unbinds from the polynucleotides in the defined time period.

2. 发明申请

WO2017013005A1 CLONING OF SINGLE-STRANDED NUCLEIC ACID 审中-公开
标题翻译：克隆单链核酸
公开(公告)号：WO2017013005A1
公开(公告)日：2017-01-26
申请号：PCT/EP2016/066876
申请日：2016-07-15
申请人： MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V.
发明人： ILIK, Ibrahim Avsar , AKHTAR, Asifa , ILIK, Tugce Aktas
IPC分类号： C12N15/10 , C12Q1/68
CPC分类号： C12N15/10 , C12Q1/68 , C12Q1/6804 , C12Q1/6853 , C12Q1/6869 , C12Q2525/121 , C12Q2525/197 , C12Q2525/204 , C12Q2525/301 , C12Q2527/107 , C12Q2522/10
摘要： The present invention relates to an oligonucleotide comprising (a) a double-stranded portion, which double-stranded portion is DNA and 9 to 30 base in length; (b) a loop connecting the 3' end of the first strand of said double-stranded portion with the 5' end of the second strand of said double-stranded portion, said loop comprising, in 5' to 3' direction: (ba) a first DNA portion which is 4 to 20 nucleotides in length; (bb) a non-nucleic acid spacer which (i) does not interfere with the formation of a stem-loop by said oligonucleotide; and (ii) causes polymerases to cease; and (be) a second DNA portion which is 4 to 20 nucleotides in length; wherein said first DNA portion and said second DNA portion are not complementary to each other; (c) a single-stranded overhang at its 5' end, said overhang being 5 to 40 nucleotides in length, wherein (ca) the bond connecting said double-stranded portion with the nucleotide of said overhang which is directly adjacent to said double-stranded portion is cleavable under alkaline conditions; and (cb) said overhang optionally comprises a barcode sequence, said barcode sequence preferably being 5 to 10 nucleotides in length; and optionally (d) within one, more or all of (a), (b) and (c), one, more or all of the following: (da) one or more modified nucleotides; (db) one or more sequences conferring compatibility with nucleic acid sequencing kits, such compatibility being preferably the presence of regions within said oligonucleotide which are complementary to primers comprised in said sequence kits; and (dc) one or more random bases.
摘要翻译：本发明涉及一种寡核苷酸，其包含（a）双链部分，其双链部分为DNA，长度为9〜30碱基; （b）将所述双股部分的第一股线的3'端与所述双股部分的第二股线的5'端连接的环，所述环包括在5'至3'方向上：（ba ）长度为4至20个核苷酸的第一个DNA部分; （bb）非核酸间隔物，其（i）不干扰所述寡核苷酸形成茎环的干扰; 和（ii）导致聚合酶停止; 和（即）长度为4至20个核苷酸的第二个DNA部分; 其中所述第一DNA部分和所述第二DNA部分彼此不互补; （c）其5'端的单链突出端，所述突出端的长度为5至40个核苷酸，其中（ca）将所述双链部分与所述突出端的核苷酸连接的键，其直接与所述双链部分相邻，绞合部分在碱性条件下可切割; 和（cb）所述突出端可选地包含条形码序列，所述条形码序列优选长度为5至10个核苷酸; （a），（b）和（c）中的一个，多个或全部中的一个，多个或全部：（da）一个或多个修饰的核苷酸; （db）赋予与核酸测序试剂盒的相容性的一个或多个序列，这种相容性优选是所述寡核苷酸内与所述序列试剂盒中包含的引物互补的区域的存在; 和（dc）一个或多个随机碱基。

3. 发明申请

WO2016139262A1 SIMULTANEOUS DETECTION OF OLIGONUCLEOTIDES, A KIT AND A USE RELATED THERETO 审中-公开
标题翻译：同时检测寡核苷酸，与其相关的用途和用途
公开(公告)号：WO2016139262A1
公开(公告)日：2016-09-09
申请号：PCT/EP2016/054450
申请日：2016-03-02
申请人： AXOLABS GMBH
发明人： RÖHL, Ingo , DÖRFLER, Nadine , KNIS, Julia
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q2523/31 , C12Q2525/204 , C12Q2525/207 , C12Q2537/143 , C12Q2545/114 , C12Q2563/107 , C12Q2565/137 , C12Q2525/107
摘要： In a first aspect, the invention relates to a method for detecting at least two distinct oligonucleotides of equal length in parallel from one biological sample, said method comprising the steps of providing a biological sample containing or suspected of containing said oligonucleotides of interest; forming a hybridization mixture using at least two fluorescently labelled detection molecules with different surface charges; separating the detection molecules hybridised to said oligonucleotides by anion exchange HPLC; and detecting the hybridized detection molecule - oligonucleotide moieties by means of quantitative fluorescence readout. In a further aspect, the invention relates to a kit comprising at least two detection molecules as defined in the present invention. In another aspect, the invention relates to the use of at least two detection molecules with different surface charges for quantitatively detecting at least two distinct oligonucleotides of equal length in parallel from one biological sample.
摘要翻译：在第一方面，本发明涉及用于从一个生物样品平行检测至少两个相同长度的不同寡核苷酸的方法，所述方法包括提供含有或怀疑含有所述感兴趣的寡核苷酸的生物样品的步骤; 使用具有不同表面电荷的至少两个荧光标记的检测分子形成杂交混合物; 通过阴离子交换HPLC分离与所述寡核苷酸杂交的检测分子; 并通过定量荧光读数检测杂交检测分子 - 寡核苷酸部分。在另一方面，本发明涉及包含本发明中定义的至少两个检测分子的试剂盒。另一方面，本发明涉及使用具有不同表面电荷的至少两个检测分子来定量检测来自一个生物样品的至少两个相同长度的不同寡核苷酸。

4. 发明申请

WO2016094148A2 METHODS FOR CAPTURING NUCLEIC ACIDS 审中-公开
标题翻译：捕获核酸的方法
公开(公告)号：WO2016094148A2
公开(公告)日：2016-06-16
申请号：PCT/US2015/063468
申请日：2015-12-02
申请人： GENERAL ELECTRIC COMPANY
发明人： NELSON, John Richard , BING, Li
IPC分类号： C12Q1/68 , C07H21/02
CPC分类号： C12Q1/6816 , C07H21/00 , C12Q1/6806 , C12Q2525/121 , C12Q2525/151 , C12Q2525/204 , C12Q2527/125 , C12Q2531/125 , C12Q2563/107 , C12Q2563/131 , C12Q2525/125 , C12Q2565/519 , C12Q2565/625
摘要： A method is provided herein, wherein the method of capturing a target nucleic acid, comprises applying a nucleic acid capture probe to a capture zone of a needs definition, wherein the nucleic acid capture probe having a first molecular weight comprises at least a sequence that is complimentary to at least a portion of the target nucleic acid sequence and the nucleic acid capture probe is substantially immobilized at the capture zone of the substrate. The method further comprises applying a sample comprising the target nucleic acid having a second molecular weight to a sample application zone of the substrate; wherein the sample comprising the target nucleic acid flows across a length of the substrate from the sample application zone to the capture zone by lateral flow, and the target nucleic acid is captured by the nucleic acid capture probes by hybridization to the capture zone.
摘要翻译：本文提供了一种捕获靶核酸的方法，包括将核酸捕获探针应用于需要定义的捕获区，其中具有第一分子量的核酸捕获探针包含至少一个序列，与靶核酸序列的至少一部分互补，并且核酸捕获探针基本上固定在基底的捕获区。所述方法还包括将包含具有第二分子量的靶核酸的样品施加到所述基底的样品施用区域; 其中包含靶核酸的样品通过横向流从样品施用区域流过基底的一段长度到捕获区，并且核酸捕获探针通过与捕获区杂交捕获靶核酸。

5. 发明申请

WO2015200541A1 DIGITAL PCR BARCODING 审中-公开
标题翻译：数字PCR BARCODING
公开(公告)号：WO2015200541A1
公开(公告)日：2015-12-30
申请号：PCT/US2015/037525
申请日：2015-06-24
申请人： BIO-RAD LABORATORIES, INC.
发明人： AGRESTI, Jeremy , COOPER, Samantha , KARLIN-NEUMANN, George , HEREDIA, Nick , LEBOFSKY, Ronald
IPC分类号： C12Q1/68
CPC分类号： C12N15/1065 , C12Q1/6806 , C12Q1/6853 , C12Q2521/531 , C12Q2525/161 , C12Q2525/204 , C12Q2535/122 , C12Q2565/629 , C12Q2563/149 , C12Q2563/159 , C12Q2563/179 , C12Q2565/514
摘要： Methods, compositions, and kits are provided for nucleic acid analysis, including single cell analysis.
摘要翻译：提供了用于核酸分析的方法，组合物和试剂盒，包括单细胞分析。

6. 发明申请

WO2015168831A1 METHODS AND COMPOSITIONS FOR DETECTING EXPRESSION OF TARGET GENES 审中-公开
标题翻译：用于检测目标基因表达的方法和组合物
公开(公告)号：WO2015168831A1
公开(公告)日：2015-11-12
申请号：PCT/CN2014/076724
申请日：2014-05-04
申请人： NINGBO HEALTH GENE TECHNOLOGIES CO., LTD.
发明人： WU, Yong , XU, Bing , WU, Linan
IPC分类号： C12Q1/68 , C12N15/11
CPC分类号： C12Q1/686 , C12Q1/6886 , C12Q2600/158 , C12Q2600/16 , C12Q2521/107 , C12Q2525/197 , C12Q2525/204 , C12Q2537/143 , C12Q2543/101 , C12Q2565/125
摘要： The present invention relates to methods and compositions, and uses thereof, for simultaneously detecting expression of multiple target genes in a sample. The present invention further relates to certain isolated polynucleotides that can be used as primers or primer pairs in the present methods and compositions for simultaneously detecting expression of multiple target genes in a sample.
摘要翻译：本发明涉及用于同时检测样品中多种靶基因表达的方法和组合物及其用途。本发明还涉及可用作本方法中的引物或引物对的某些分离的多核苷酸，同时检测样品中多种靶基因的表达的组合物。

7. 发明申请

WO2015164635A2 METHODS AND COMPOSITIONS FOR DETECTING ANTI-DRUG ANTIBODIES 审中-公开
标题翻译：检测抗药物抗体的方法和组合物
公开(公告)号：WO2015164635A2
公开(公告)日：2015-10-29
申请号：PCT/US2015/027341
申请日：2015-04-23
申请人： ALNYLAM PHARMACEUTICALS, INC.
发明人： GAO, Minggeng , HUTABARAT, Renta
IPC分类号： G01N33/53
CPC分类号： C12Q1/6804 , C07K16/44 , C07K2317/22 , C07K2317/33 , C12Q1/68 , G01N33/5308 , C12Q2525/117 , C12Q2525/204 , C12Q2565/525
摘要： Assays, methods, reagents and kits for evaluating the level of an antibody against a nucleic acid molecule, e.g ., a double- stranded oligonucleotide or RNA molecule (e.g ., dsRNA), are disclosed herein.
摘要翻译：用于评估针对核酸分子例如双链寡核苷酸或RNA分子（（例如））的抗体水平的试验，方法，试剂和试剂盒例如dsRNA）在本文中公开。

8. 发明申请

WO2014114922A1 METHODS FOR ESTIMATING THE SIZE OF DISEASE-ASSOCIATED POLYNUCLEOTIDE REPEAT EXPANSIONS IN GENES 审中-公开
标题翻译：估计疾病相关多糖重复扩增大小的方法
公开(公告)号：WO2014114922A1
公开(公告)日：2014-07-31
申请号：PCT/GB2014/050148
申请日：2014-01-20
申请人： MEDICAL RESEARCH COUNCIL
发明人： MEAD, Simon , POULTER, Mark , BECK, Jonathan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/683 , C12Q2600/158 , C12Q2525/151 , C12Q2525/204 , C12Q2537/16
摘要： Methods for estimating the size of disease-associated polynucleotide repeat expansions in genes are disclosed which use restriction enzymes that do not cut within a repeat expansion and which are frequent cutting restriction enzymes that cut genomic DNA outside of the expansion into fragments of a size below the threshold capable of detection. A hybridisation probe that can bind to multiple sites within the expansion is then used to estimate its length and to correlate that to the diagnosis or prognosis of disease.
摘要翻译：公开了用于估计基因中与疾病相关的多核苷酸重复扩增的大小的方法，其使用不在重复扩增中切割的限制酶，并且是频繁切割限制酶，其将扩增外的基因组DNA切割成低于能够检测的阈值。然后可以使用可以结合扩增内的多个位点的杂交探针来估计其长度并将其与疾病的诊断或预后相关联。

9. 发明申请

WO2014068020A1 METHOD FOR THE SIMULTANEOUS AMPLIFICATION OF A PLURALITY OF DIFFERENT NUCLEIC ACID TARGET SEQUENCES 审中-公开
标题翻译：同时扩增不同浓度的核酸目标序列的方法
公开(公告)号：WO2014068020A1
公开(公告)日：2014-05-08
申请号：PCT/EP2013/072749
申请日：2013-10-30
申请人： UNIVERSITÄTSSPITAL BASEL
发明人： KINTER, Jochen , SINNREICH, Michael
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6858 , C12Q1/6888 , C12Q2600/16 , C12Q2525/204 , C12Q2537/143 , C12Q2549/119
摘要： The present invention relates to a method for the simultaneous amplification of a plurality of different nucleic acid target sequences comprising the steps of providing a plurality of different nucleic acid polymers as templates, each template comprising a specific target sequence and a primer annealing sequence located downstream of the target sequence, and amplifying the template by a polymerase dependent amplification reaction using a primer oligonucleotide comprising a primer sequence which is at least essentially complementary to the primer annealing sequence. The method is characterized in that for the polymerase dependent amplification reaction a set of primer oligonucleotides is used, said set comprising at least two primer oligonucleotides which are able to anneal to the primer annealing sequence of the same template and which differ from each other in the efficiency for the polymerase dependent amplification reaction to take place.
摘要翻译：本发明涉及同时扩增多个不同核酸靶序列的方法，包括以下步骤：提供多种不同的核酸聚合物作为模板，每个模板包含特定的靶序列和位于靶序列，并使用包含与引物退火序列至少基本上互补的引物序列的引物寡核苷酸，通过聚合酶依赖性扩增反应扩增模板。该方法的特征在于，对于聚合酶依赖性扩增反应，使用一组引物寡核苷酸，所述组包含至少两个引物寡核苷酸，其能够退火到相同模板的引物退火序列，并且在聚合酶依赖性扩增反应发生的效率。

10. 发明申请

WO2013132305A8 SIZE-BASED ANALYSIS OF FETAL DNA FRACTION IN MATERNAL PLASMA 审中-公开
标题翻译：母婴血浆中胎儿DNA分数的基于尺度的分析
公开(公告)号：WO2013132305A8
公开(公告)日：2013-11-21
申请号：PCT/IB2013000312
申请日：2013-03-08
申请人： UNIV HONG KONG CHINESE
发明人： LO YUK MING DENNIS , CHAN KWAN CHEE , ZHENG WENLI , JIANG PEIYONG , LIAO JIAWEI , CHIU WAI KWUN ROSSA
IPC分类号： C12Q1/68 , G06F19/18 , G06F19/20
CPC分类号： G06F19/22 , C12Q1/6809 , C12Q1/6816 , C12Q1/6827 , C12Q1/6869 , G06F19/18 , G06F19/3431 , G06F19/345 , G16H50/20 , G16H50/30 , C12Q2535/122 , C12Q2537/16 , C12Q2537/165 , C12Q2539/107 , C12Q2525/204
摘要： A fractional concentration of clinically-relevant DNA in a mixture of DNA from a biological sample is determined based on amounts of DNA fragments at multiple sizes. For example, the fractional concentration of fetal DNA in maternal plasma or tumor DNA in a patient's plasma can be determined. The size of DNA fragments in a sample is shown to be correlated with a proportion of fetal DNA and a proportion of tumor DNA, respectively. Calibration data points (e.g., as a calibration function) indicate a correspondence between values of a size parameter and the fractional concentration of the clinically-relevant DNA. For a given sample, a first value of a size parameter can be determined from the sizes of DNA fragments in a sample. A comparison of the first value to the calibration data points can provide the estimate of the fractional concentration of the clinically-relevant DNA.
摘要翻译：基于多种大小的DNA片段的量确定来自生物样品的DNA的混合物中的临床相关DNA的分数浓度。例如，可以确定患者血浆中母体血浆或肿瘤DNA中胎儿DNA的分数浓度。显示样品中DNA片段的大小分别与胎儿DNA的比例和肿瘤DNA的比例相关。校准数据点（例如，作为校准函数）指示尺寸参数的值与临床相关DNA的分数浓度之间的对应关系。对于给定的样品，尺寸参数的第一个值可以根据样品中DNA片段的大小确定。第一个值与校准数据点的比较可以提供临床相关DNA分数浓度的估计值。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式