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1. 发明申请

WO1995018971A1 METHODS FOR THE SOLID PHASE SYNTHESIS OF GLYCOCONJUGATES 审中-公开
标题翻译：甘氨酸钠固相合成方法
公开(公告)号：WO1995018971A1
公开(公告)日：1995-07-13
申请号：PCT/US1995000484
申请日：1995-01-10
申请人： AFFYMAX TECHNOLOGIES N.V. , VETTER, Dirk , TUMELTY, David, A. , ANTONENKO, Valery
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： G01N33/543
CPC分类号： C07H13/04 , C07H3/04 , C07H3/06 , C07K1/04 , C07K1/047 , C07K9/00
摘要： An efficient and versatile method of forming N-linked glycoconjugates is described wherein a glycosyl acceptor, typically comprising an activated carboxyl group, is reacted with a glycosylating agent, typically a glycosylamine, in the presence of a coupling catalyst and optionally an exogenous base. Depending on the choice of reactive site, this method can be utilized to form N-linked glycoconjugates, in either a soluble or substrate-bound, linear or branched format.
摘要翻译：描述了形成N-连接的糖缀合物的有效和通用的方法，其中通常包含活化的羧基的糖基受体与糖基化剂（通常为糖胺）在偶联催化剂和任选的外源碱的存在下反应。根据反应位点的选择，该方法可用于以可溶或底物结合的线性或分支形式形成N-连接的糖缀合物。

2. 发明申请

WO1995011922A1 IN VITRO PEPTIDE AND ANTIBODY DISPLAY LIBRARIES 审中-公开
标题翻译：在体外肽和抗体显示图书馆
公开(公告)号：WO1995011922A1
公开(公告)日：1995-05-04
申请号：PCT/US1994012206
申请日：1994-10-25
申请人： AFFYMAX TECHNOLOGIES N.V. , MATTHEAKIS, Larry, C. , DOWER, William, J.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C07K16/00
CPC分类号： C12N15/1041 , C07K16/00 , C07K16/1285 , C07K2317/34
摘要： Improved methods and novel compositions for identifying peptides and single-chain antibodies that bind to predetermined receptors or epitopes. Such peptides and antibodies are indentified by improved and novel methods for affinity screening of polysomes displaying nascent peptides.
摘要翻译：用于鉴定结合预定受体或表位的肽和单链抗体的改进方法和新型组合物。通过用于亲和筛选显示新生肽的多核糖体的改进和新颖的方法来鉴定此类肽和抗体。

3. 发明申请

WO1993010161A1 POLYMER REVERSAL ON SOLID SURFACES 审中-公开
标题翻译：聚合物反映固体表面
公开(公告)号：WO1993010161A1
公开(公告)日：1993-05-27
申请号：PCT/US1992009964
申请日：1992-11-19
申请人： AFFYMAX TECHNOLOGIES N.V. , HOLMES, Christopher, P.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C08F283/00
CPC分类号： B82Y30/00 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , C07B2200/11 , C07H21/00 , C07K1/04 , C07K1/042 , C40B40/06 , C40B40/10 , C40B60/14 , G03F7/00 , Y02P20/55
摘要： A method for cyclization and reversal of the polarity of polymers on a substrate. The method provides for the formation of a polymer on a substrate (2) with a tether molecule (4). Through unmasking of a protective group (PG2) a cyclic polymer (6) is formed. Through cleavage of an appropriate bond, a polarity reversed polymer (8) is formed. The method finds particular application in the formation of, for example, peptides and oligonucleotides.

4. 发明申请

WO1993008278A1 PEPTIDE LIBRARY AND SCREENING METHOD 审中-公开
标题翻译：肽库和筛选方法
公开(公告)号：WO1993008278A1
公开(公告)日：1993-04-29
申请号：PCT/US1992008879
申请日：1992-10-15
申请人： AFFYMAX TECHNOLOGIES N.V. , SCHATZ, Peter, J. , CULL, Millard, G. , MILLER, Jeff, F. , STEMMER, Willem, Peter, Christian
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C12N15/10
CPC分类号： G01N33/68 , C12N15/1075
摘要： A random peptide library constructed by transforming host cells with a collection of recombinant vectors that encode a fusion protein comprised of a DNA binding protein and a random peptide and also contain a binding site for the DNA binding protein can be used to screen for novel ligands. The screening method results in the formation of a complex comprising the fusion protein bound to a receptor through the random peptide ligand and to the recombinant DNA vector through the DNA binding protein.

5. 发明申请

WO1992010588A1 SEQUENCING BY HYBRIDIZATION OF A TARGET NUCLEIC ACID TO A MATRIX OF DEFINED OLIGONUCLEOTIDES 审中-公开
标题翻译：通过将目标核酸杂交到定义的寡核苷酸的矩阵进行测序
公开(公告)号：WO1992010588A1
公开(公告)日：1992-06-25
申请号：PCT/US1991009226
申请日：1991-12-06
申请人： AFFYMAX TECHNOLOGIES N.V. , FODOR, Stephen, P., A. , SOLAS, Dennis, W. , DOWER, William, J.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C12Q01/68
CPC分类号： G03F7/00 , B01J19/0046 , B01J2219/00315 , B01J2219/00432 , B01J2219/00434 , B01J2219/00436 , B01J2219/00459 , B01J2219/00468 , B01J2219/00475 , B01J2219/005 , B01J2219/00527 , B01J2219/00529 , B01J2219/00531 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00648 , B01J2219/00659 , B01J2219/00689 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B82Y10/00 , B82Y30/00 , C07B2200/11 , C07H19/10 , C07H21/00 , C07K1/042 , C07K1/045 , C07K17/06 , C07K17/14 , C12Q1/6804 , C12Q1/6809 , C12Q1/6816 , C12Q1/6827 , C12Q1/6834 , C12Q1/6837 , C12Q1/6874 , C40B40/06 , C40B40/10 , C40B60/14 , G01N15/1475 , G03F7/265 , G03F7/38 , G11C13/0014 , G11C13/0019 , C12Q2565/513 , C12Q2565/507 , C12Q2545/101 , C12Q2525/185 , C12Q2565/514 , C12Q2563/173 , C12Q2563/119 , C12Q2563/149 , C12Q2525/151 , C12Q2525/179 , C12Q2525/204
摘要： The present invention provides methods and appartus for sequencing, fingerprinting and mapping biological polymers, particularly polynucleotides. The methods make use of a plurality of positionally distinct sequence specific recognition reagents, such as polynucleotides. The apparatus employs a substrate comprising positionally distinct sequence specific recognition reagents, such as polynucleotides, which are preferably localized at high densities. The methods and apparatus of the present invention can be used for determining the sequence of polynucleotides, mapping polynucleotides, and developing polynucleotide fingerprints. Polynucleotide fingerprints can be used for identifying individuals, tissue samples, pathological conditions, genetic diseases, infectious diseases, and other applications. Polynucleotide fingerprints can also be used for classification of bioligical samples, including taxonomy, and to characterize their sources. The invention also provides polynucleotide mapping, fingerprinting, and sequencing as valuable laboratory research tools for use in biological investigations.
摘要翻译：本发明提供了用于测序，指纹和映射生物聚合物，特别是多核苷酸的方法和应用。所述方法利用多种位置不同的序列特异性识别试剂，例如多核苷酸。该装置使用包含位置不同的序列特异性识别试剂（例如多核苷酸）的底物，其优选以高密度定位。本发明的方法和装置可以用于确定多核苷酸序列，映射多核苷酸和开发多核苷酸指纹。多核苷酸指纹可用于鉴定个体，组织样本，病理状况，遗传疾病，传染病和其他应用。多核苷酸指纹也可用于生物样本的分类，包括分类学，并对其来源进行表征。本发明还提供多核苷酸定位，指纹和测序作为用于生物学研究的有价值的实验室研究工具。

6. 发明申请

WO1990015070A1 VERY LARGE SCALE IMMOBILIZED PEPTIDE SYNTHESIS 审中-公开
标题翻译：非常大的规模固定肽合成
公开(公告)号：WO1990015070A1
公开(公告)日：1990-12-13
申请号：PCT/NL1990000081
申请日：1990-06-07
申请人： AFFYMAX TECHNOLOGIES N.V.
发明人： AFFYMAX TECHNOLOGIES N.V. , PIRRUNG, Michael, C. , READ, J., Leighton , FODOR, Stephen, P., A. , STRYER, Lubert
IPC分类号： C07K01/04
CPC分类号： C08G69/00 , B01J19/0046 , B01J2219/00315 , B01J2219/00432 , B01J2219/00434 , B01J2219/00436 , B01J2219/00459 , B01J2219/00468 , B01J2219/00475 , B01J2219/005 , B01J2219/00527 , B01J2219/00529 , B01J2219/00531 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00639 , B01J2219/00648 , B01J2219/00659 , B01J2219/00689 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01L7/52 , B82Y10/00 , B82Y30/00 , C07B2200/11 , C07C229/14 , C07C229/16 , C07D263/44 , C07D317/62 , C07H19/04 , C07H19/10 , C07H21/00 , C07K1/04 , C07K1/042 , C07K1/045 , C07K1/047 , C07K1/062 , C07K7/06 , C07K17/06 , C07K17/14 , C12Q1/6809 , C12Q1/6816 , C12Q1/6837 , C12Q1/6874 , C40B40/06 , C40B40/10 , C40B60/14 , C40B80/00 , G01N15/1475 , G01N21/253 , G01N21/6428 , G01N21/6452 , G03F7/00 , G03F7/26 , G03F7/265 , G03F7/38 , G11C13/0014 , G11C13/0019 , H01L25/50 , H01L2924/0002 , Y02P20/55 , Y10S435/961 , Y10S435/968 , Y10S435/969 , Y10S435/973 , Y10S436/807 , Y10S436/809 , Y10T428/31971 , Y10T436/2575 , H01L2924/00
摘要： A method and device for preparing desired sequences on a substrate at known locations. Known locations (10) of a substrate (2) are irradiated by way of a mask (8) so as to activate a material (4) for binding. The substrate is then exposed to a first material (12) for binding thereto. Second locations (14) are then irradiated through a mask and exposed to a second material (16). A variety of sequences may be formed through selective irradiation of the substrate followed by application of selected materials. A reactor system and fluorescence detection system are also disclosed.

7. 发明申请

WO1996040987A1 PEPTIDE LIBRARY AND SCREENING METHOD 审中-公开
标题翻译：肽库和筛选方法
公开(公告)号：WO1996040987A1
公开(公告)日：1996-12-19
申请号：PCT/US1996009809
申请日：1996-06-07
申请人： AFFYMAX TECHNOLOGIES N.V. , SCHATZ, Peter, J. , CULL, Millard, G. , MILLER, Jeff, F. , STEMMER, Willem, P., C. , GATES, Christian, M.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C12Q01/66
CPC分类号： C12N15/1075 , C12Q1/6811
摘要： A random peptide library constructed by transforming host cells with a collection of recombinant vectors that encode a fusion protein comprised of a DNA binding protein and a random peptide and also encode a binding site for the DNA binding protein can be used to screen for novel ligands. The screening method results in the formation of a complex comprising the fusion protein bound to a receptor through the random peptide ligand and to the recombinant DNA vector through the DNA binding protein.
摘要翻译：通过用编码由DNA结合蛋白和随机肽组成的融合蛋白的重组载体的集合转化宿主细胞构建的随机肽文库，并且编码DNA结合蛋白的结合位点可用于筛选新配体。筛选方法导致通过DNA结合蛋白形成包含通过随机肽配体与受体结合的融合蛋白和重组DNA载体的复合物。

8. 发明申请

WO1995035278A1 METHODS FOR SYNTHESIZING DIVERSE COLLECTIONS OF PYRROLIDINE COMPOUNDS 审中-公开
标题翻译：合成吡咯啉化合物的多样性收集方法
公开(公告)号：WO1995035278A1
公开(公告)日：1995-12-28
申请号：PCT/US1995007878
申请日：1995-06-22
申请人： AFFYMAX TECHNOLOGIES N.V. , GALLOP, Mark, A. , MURPHY, Martin, A.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C07D207/12
CPC分类号： C07D207/16 , B01J2219/00592 , B01J2219/0072
摘要： Disclosed are methods for synthesizing very large collections of diverse pyrrolidine compounds and derivatives thereof on solid supports and synthetic compound libraries comprising pyrrolidine groups and derivatives thereof prepared by such methods.
摘要翻译：公开了用于在固体支持物上合成非常大量的各种吡咯烷化合物及其衍生物的方法，其包含通过这些方法制备的吡咯烷基团及其衍生物的合成化合物文库。

9. 发明申请

WO1995033846A1 BIOARRAY CHIP REACTION APPARATUS AND ITS MANUFACTURE 审中-公开
标题翻译：生物芯片反应装置及其制造
公开(公告)号：WO1995033846A1
公开(公告)日：1995-12-14
申请号：PCT/US1995007377
申请日：1995-06-08
申请人： AFFYMAX TECHNOLOGIES N.V. , BESEMER, Donald, M. , GOSS, Virginia, W. , WINKLER, James, L.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C12Q01/00
CPC分类号： B01L3/502715 , B01F13/0059 , B01F13/02 , B01F13/08 , B01J19/0046 , B01J2219/00371 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00531 , B01J2219/00536 , B01J2219/0054 , B01J2219/00547 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00659 , B01J2219/00662 , B01J2219/00689 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L3/5027 , B01L3/502707 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0689 , B01L2200/12 , B01L2300/0636 , B01L2300/0816 , B01L2300/0825 , B01L2300/0877 , B01L2400/0487 , B82Y30/00 , C07H21/00 , C12Q1/6874 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , G01N33/543 , G01N33/54366 , G01N2035/00158 , Y10S435/97 , Y10S435/973 , Y10S436/809 , Y10T436/112499
摘要： A body (2700) having a cavity (2710) for mounting a substrate (2790) fabricated with probe sequences (2795) at known locations according to the methods disclosed in United States Patent Number 5,153,854 and PCT WO 92/10092, for example, is provided. The cavity (2710) includes inlets (2750 and 2751) for introducing selected fluids into the cavity (2710) to contact the probes (2795). Accordingly, a commercially feasible device (2700) for use in high throughput assay systems is provided.
摘要翻译：例如，根据美国专利号5,153,854和PCT WO 92/10092中公开的方法，在已知位置处具有用于安装用探针序列（2795）制成的基底（2790）的腔体（2700）是提供。空腔（2710）包括用于将选定流体引入空腔（2710）中以接触探针（2795）的入口（2750和2751）。因此，提供了用于高通量测定系统的商业上可行的装置（2700）。

10. 发明申请

WO1995022058A1 METHOD AND APPARATUS FOR DETECTION OF FLUORESCENTLY LABELED MATERIALS 审中-公开
标题翻译：用于检测荧光标签材料的方法和装置
公开(公告)号：WO1995022058A1
公开(公告)日：1995-08-17
申请号：PCT/US1995001886
申请日：1995-02-09
申请人： AFFYMAX TECHNOLOGIES N.V. , STERN, David , FIEKOWSKY, Peter , TRULSON, Mark
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： G01N33/552
CPC分类号： G01N21/6458 , B01J2219/00605 , B01J2219/00612 , B01J2219/00659 , B01J2219/00702 , G01N21/6428 , G01N2021/174
摘要： Fluorescently marked targets bind to a substrate (230) synthesized with polymer sequences at known locations. The substrate (230) is mounted on an x-y-z translation table (240) during detection. The targets are detected by exposing selected regions of the substrate (230) to light from a light source (100) and detecting the light photons fluoresced therefrom, moving the substrate (230) position, and repeating the exposure and detection steps until the substrate (230) has been completely examined. The device includes a controller (190) for controlling the x-y-z translation table (240) movement for exposure of the selected regions of the substrate (230), the detection of the fluoresced light, and positioning of the substrate (230) to provide proper focusing of the light on the substrate (230) for maximum sensitivity. The resulting data can be used to determine binding affinity of the targets to the specific polymer sequences.
摘要翻译：荧光标记的靶结合到在已知位置处与聚合物序列合成的底物（230）。在检测期间，将衬底（230）安装在x-y-z平移台（240）上。通过将衬底（230）的选定区域暴露于来自光源（100）的光并检测从其发荧光的光子，移动衬底（230）位置并重复曝光和检测步骤直到衬底（ 230）已经完全检查。该装置包括控制器（190），用于控制用于曝光衬底（230）的选定区域的xyz平移台（240）运动，荧光灯的检测和衬底（230）的定位以提供适当的聚焦的基板（230）上的光以获得最大的灵敏度。所得数据可用于确定靶向特定聚合物序列的结合亲合力。

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