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1. 发明申请

WO2018229018A1 METHOD OF MEASURING VISCOSITY IN A MICROFLUIDIC SYSTEM 审中-公开
公开(公告)号：WO2018229018A1
公开(公告)日：2018-12-20
申请号：PCT/EP2018/065407
申请日：2018-06-11
申请人： BIOMILLENIA SAS
发明人： DAUKOVIC, Aleksander
IPC分类号： G01N11/00 , A61B5/00 , B01L3/06 , C12Q1/6806 , G01N33/49 , G01N33/52
摘要： The invention relates to a microfluidic method for measuring viscosity in a micro droplet in a microfluidic system, comprising the steps of i) introducing a fluorescent molecule into a micro droplet otherwise comprising a fluid, ii) in the microfluidic system, exciting the fluorescent molecule in said micro droplet by applying light to the micro droplet, iii) measuring the resulting fluorescence emitted from the micro droplet thereby determining the viscosity of the fluid in the micro droplet. The invention also relates to method of screening for microorganisms or cells that produce viscosity‐ modulating compounds with desired properties. Finally, the invention also relates to the use of fluorescent molecules for measuring the viscosity of a fluid in a micro droplet in a microfluidic system.

2. 发明申请

WO2018078129A1 PREPARING AND ANALYZING SOLID FORM PROPERTIES OF A SUBSTANCE 审中-公开
标题翻译：一种物质的固体形态的制备和分析
公开(公告)号：WO2018078129A1
公开(公告)日：2018-05-03
申请号：PCT/EP2017/077665
申请日：2017-10-27
申请人： F. HOFFMANN-LA ROCHE AG , HOFFMANN-LA ROCHE INC.
发明人： SCHWITTER, Urs , RAN, Frédéric , ALKER, André , KISSLING, Tom , ZUMSTEIN, Thomas
IPC分类号： B01L3/00 , B01L3/06
摘要： In a method of analyzing solid form properties of a substance, which comprising the step of solidifying the substance, the solidified substance is obtained in one of a plurality of wells (1, 46) of a multi-well plate (4). In the multi-well plate (4) the at least one of the plurality of wells (1, 46) has a bottom (22) made of a thermoplastic polyimide. The method further comprises analyzing the solidified substance in the well (1, 46) of the multi-well plate (4) by X-ray diffraction. Thereby, said analysis comprises providing X-ray through the solidified substance and a bottom (22) of the well (1, 46) and evaluating the X-ray which passed the solidified substance and the bottom (22) of the well (1, 46). Such method and multi-well plate (4) provide a durable and cost efficient system allowing a high quality analysis of solid form properties of the substance and an efficient and safe processing of the substance.
摘要翻译：在分析包括固化物质的步骤的物质的固体形态性质的方法中，固化的物质在多级多孔体（1,46）中的一个中获得，（4）。在多孔板（4）中，多个井（1,46）中的至少一个具有由热塑性聚酰亚胺制成的底部（22）。该方法还包括通过X射线衍射分析多孔板（4）的孔（1,46）中的固化物质。因此，所述分析包括提供穿过固化物质和井（1,46）的底部（22）的X射线并且评估穿过固化物质和井（1，46）的底部（22）的X射线， 46）。这种方法和多孔板（4）提供了耐用且成本有效的系统，允许高质量地分析该物质的固体形式特性以及该物质的有效且安全的处理。

3. 发明申请

WO2010037510A1 METHOD OF LOADING A CRYSTALLIZATION DEVICE 审中-公开
标题翻译：装载结晶装置的方法
公开(公告)号：WO2010037510A1
公开(公告)日：2010-04-08
申请号：PCT/EP2009/006964
申请日：2009-09-28
申请人： QIAGEN GMBH , KUBICEK, Johann , SCHÄFER, Frank , LABAHN, Jörg , BÜLDT, Georg , FORSCHUNGSZENTRUM JÜLICH GMBH(FJZ)
发明人： KUBICEK, Johann , SCHÄFER, Frank , LABAHN, Jörg , BÜLDT, Georg
IPC分类号： B01D9/00 , C30B7/00 , C30B29/58 , B01L3/06
CPC分类号： C07K1/306 , B01D9/00 , B01D9/0077 , B01D2009/0086 , B01L3/06 , C07K14/705
摘要： The present invention pertains to a method for loading a crystallization device and for manufacturing a crystallization device comprising multiple receptacles with a pre-defined amount of at least one matrix-forming compound capable of forming a crystallization matrix for a membrane protein, said method comprising the following steps: a) Modifying the state of aggregation of said at least one matrix-forming compound to a fluidic state which allows dispensing said at least one matrix-forming compound, and b) dispensing a defined amount of said at least one matrix-forming compound into at least one receptacle of the crystallization device, wherein said dispensed matrix-forming compound solidifies within said receptacle. Thereby, prefilled crystallization devices are obtained which can be used as consumables in particular in automated crystallization processes. Also provided are protein crystallization methods using respectively prepared crystallization devices.
摘要翻译：本发明涉及一种用于装载结晶装置和用于制造结晶装置的方法，所述结晶装置包括具有预定量的至少一种能够形成膜蛋白质的结晶基质的基质形成化合物的多个容器，所述方法包括以下步骤：a）将所述至少一种基质形成化合物的聚集状态改变为允许分配所述至少一种基质形成化合物的流体状态，以及b）分配限定量的所述至少一种基质形成化合物进入结晶装置的至少一个容器中，其中所述分配的基质形成化合物在所述容器内固化。因此，获得了可以用作消费品，特别是在自动结晶过程中的预填充结晶装置。还提供了使用分别制备的结晶装置的蛋白质结晶方法。

4. 发明申请

WO2009150549A3 MICROFLUIDIC DEVICES AND METHODS FOR PROTEINS CRYSTALLIZATION AND IN SITU X-RAY DIFFRACTION 审中-公开
标题翻译：蛋白质结晶和X射线衍射的微流体装置和方法
公开(公告)号：WO2009150549A3
公开(公告)日：2010-04-01
申请号：PCT/IB2009006586
申请日：2009-06-15
申请人： SPINX INC , ZUCCHELLI PIERO
发明人： ZUCCHELLI PIERO
IPC分类号： B01L3/06 , B01D9/00 , B01L3/00 , C30B7/00 , C30B29/58
CPC分类号： B01L3/06 , B01D9/00 , B01D9/0072 , B01D9/0077 , B01L3/5025 , B01L3/5027 , B01L2200/0621 , B01L2200/10 , B01L2200/12 , B01L2300/0816 , B01L2300/0819 , B01L2300/0854 , B01L2300/087 , B01L2300/0887 , B01L2400/0409 , B01L2400/0683 , C30B7/00 , C30B29/58
摘要： The present disclosure is directed generally to devices and methods with the purpose of interfacing micro fluidic devices with dispensing and fluid handling systems to achieve the rapid identification of protein crystallization conditions. The device described herein is fabricated with the use of a cyclic olefin homopolymer-based creating microfluidics system adaptable for protein crystallization and in situ X-ray diffraction. Connectivity between chambers is controlled by valves that allow specified volumes of liquid to be transferred from one chamber to another. The microfluidic system is useful to established microbatch, vapor diffusion and free interface diffusion protocols for protein crystallization and to obtain crystals for a number of proteins, including chicken lysozyme, bovine trypsin, a human p53 protein containing both the DNA binding and oligomerization domains bound to DNA and a functionally important domain of Arabidopsis Morpheus' Molecule 1 (MOMl). For X-ray diffraction analysis, either the microfluidic devices were opened to allow mounting of the crystals on loops or the crystals were exposed to X-rays in situ. Thus, cyclic olefin homopolymer-based microfluidics systems are useful to further automate protein crystallization and structural genomics efforts.
摘要翻译：本公开通常涉及用于将微流体装置与分配和流体处理系统接合以实现蛋白质结晶条件的快速鉴定的装置和方法。本文描述的装置使用适用于蛋白质结晶和原位X射线衍射的基于环烯烃均聚物的产生微流体系统来制造。室之间的连通性由阀控制，允许指定体积的液体从一个室转移到另一个室。微流体系统可用于建立蛋白质结晶的微批，蒸汽扩散和自由界面扩散方案，并获得许多蛋白质的晶体，包括鸡溶菌酶，牛胰蛋白酶，含有结合到DNA结合和寡聚结构域的人p53蛋白 DNA和功能重要的拟南芥Morpheus分子1（MOM1）的结构域。对于X射线衍射分析，打开微流体装置以允许将晶体安装在环上或将晶体原位暴露于X射线。因此，环烯烃均聚物基微流体系统可用于进一步自动化蛋白质结晶和结构基因组学研究。

5. 发明申请

WO2006005376A9 LIQUID TRANSPORTATION AND CRYSTALLIZATION GROWTH 审中-公开
标题翻译：液体运输和结晶生长
公开(公告)号：WO2006005376A9
公开(公告)日：2006-03-16
申请号：PCT/EP2004051505
申请日：2004-07-15
申请人： AGILENT TECHNOLOGIES INC , DEHMER BERNHARD
发明人： DEHMER BERNHARD
IPC分类号： B01F13/00 , B01L3/00 , B01L3/06 , C30B7/00 , H01J49/16
CPC分类号： B01L3/502746 , B01L3/06 , B01L3/502707 , B01L3/50273 , B01L3/50857 , B01L2300/048 , B01L2300/161 , B01L2400/0406 , C30B7/00
摘要： A device for transporting liquids and supporting crystal growth comprises a hollow space (20) in a body (1) with a first side. The hollow space comprises at least a first orifice (9) and is being adapted for generating a directed capillary ascension effect towards the at least first orifice (9).
摘要翻译：一种用于输送液体并支持晶体生长的装置包括在具有第一侧的主体（1）中的中空空间（20）。中空空间至少包括第一孔（9）并且适于朝着至少第一孔口（9）产生定向的毛细管提升效应。

6. 发明申请

WO02042731A2 METHODS AND DEVICES FOR HIGH THROUGHPUT CRYSTALLIZATION 审中-公开
标题翻译：用于高通量结晶的方法和装置
公开(公告)号：WO02042731A2
公开(公告)日：2002-05-30
申请号：PCT/US2001/042968
申请日：2001-11-20
IPC分类号： B01J19/00 , B01L3/00 , B01L3/06 , C30B7/00 , C40B60/14 , G01N
CPC分类号： B01J19/0046 , B01J2219/00274 , B01J2219/00313 , B01J2219/0043 , B01J2219/00443 , B01J2219/00585 , B01J2219/00659 , B01J2219/00707 , B01J2219/00756 , B01L3/06 , B01L3/5085 , B01L2300/0896 , B01L2300/16 , B82Y15/00 , B82Y30/00 , C30B7/00 , C30B29/58 , C40B60/14
摘要： Crystallization apparatus (20) and methods which allow for fast screening and determination of protein crystallization conditions with small protein quantities and rapid crystallization. The apparatus comprise a first region comprising a first nucleation catalyst material (36) and a second region comprising a second nucleation catalyst material (38), with the first and second regions positioned adjacent to each other and configured to support at least one crystal, and with the first region having a variation in a nucleation property of the first nucleation catalyst material (36), in the first region. The crystal may be supported at an interface of the adjacent regions. The methods comprise providing a first region of a first nucleation catalyst material (36) and a second region of a second nucleation catalyst material (38) adjacent said first region, with the first region having a variation in a nucleation property of the first nucleation catalyst material (36), exposing the first and second regions to a solution of a selected molecule, and growing at least one crystal of the molecule in association with the first and second regions.
摘要翻译：结晶装置（20）和允许快速筛选和测定具有小蛋白质量和快速结晶的蛋白质结晶条件的方法。该装置包括第一区域，其包括第一成核催化剂材料（36）和包含第二成核催化剂材料（38）的第二区域，其中第一和第二区域彼此相邻并且被配置为支持至少一个晶体，以及其中第一区域在第一区域具有第一成核催化剂材料（36）的成核性质的变化。晶体可以在相邻区域的界面处被支撑。所述方法包括提供第一成核催化剂材料（36）的第一区域和邻近所述第一区域的第二成核催化剂材料（38）的第二区域，其中第一区域具有第一成核催化剂的成核性能的变化材料（36），将所述第一和第二区域暴露于所选择的分子的溶液，并且与所述第一和第二区域相关联地生长所述分子的至少一个晶体。

7. 发明申请

WO01075415A2 PROTEIN CRYSTALLIZATION IN MICROFLUIDIC STRUCTURES 审中-公开
标题翻译：微流体结构蛋白质结晶
公开(公告)号：WO01075415A2
公开(公告)日：2001-10-11
申请号：PCT/US2001/010565
申请日：2001-03-30
IPC分类号： C12M1/00 , B01D9/02 , B01F13/00 , B01J19/00 , B01L3/00 , B01L3/06 , B01L7/00 , C30B7/00 , C30B27/00 , C30B30/08 , G01N25/14 , G01N35/00 , G01N
CPC分类号： B01L7/54 , B01F13/0059 , B01F13/0093 , B01L3/06 , B01L3/5025 , B01L3/50273 , B01L3/502776 , B01L2200/0636 , B01L2200/0684 , B01L2300/0816 , B01L2300/0867 , B01L2400/0481 , B01L2400/0487 , B01L2400/0605 , C30B7/00 , C30B27/00 , C30B29/58 , C30B30/08 , G01N25/14 , G01N2035/00336 , Y10S117/90 , Y10T117/10 , Y10T117/1024
摘要： A device for promoting protein crystal growth (PCG) using microfluidic channels. A protein sample and a solvent solution are combined within a microfluidic channel having laminar flow characteristics which forms diffusion zones, providing for a well defined crystallization. Protein crystals can then be harvested from the device. The device is particularly suited for microgravity conditions.
摘要翻译：使用微流体通道促进蛋白质晶体生长（PCG）的装置。蛋白质样品和溶剂溶液在具有形成扩散区的层流特性的微流体通道内组合，提供良好限定的结晶。然后可以从装置中收获蛋白质晶体。该器件特别适用于微重力条件。

8. 发明申请

WO2022165422A1 2D POLYMER BASED TARGETS FOR SERIAL X-RAY CRYSTALLOGRAPHY 审中-公开
公开(公告)号：WO2022165422A1
公开(公告)日：2022-08-04
申请号：PCT/US2022/014748
申请日：2022-02-01
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人： KUHL, Tonya L. , GILBILE, Deepshika Shamraj
IPC分类号： G01N23/20 , B01L3/00 , B01L3/06 , G01N23/00 , G01N23/20025 , G01N23/201
摘要： Systems and methods for producing and using polymer based fixed targets for hydrated, room temperature, high-throughput serial protein crystallography with minimal background and minimal sample consumption are provided. The fixed targets can also be used with non-protein samples such as nanoparticle inorganic crystals. The targets maintain full crystal hydration that allows for on-chip crystallization as well as maintaining long term stability of the sample within the chip. The target chips are optimized to minimize background by controlling the sample thickness in the beam to match crystal size, enabling fast raster scanning at lower fidelity and high hit rates that facilitate dynamic measurements. The targets provide an inexpensive, flexible substrate for on-chip, micro-batch or vapor diffusion crystallization facilitated by polymer brushes for sample concentration and surface initiated crystallization thereby eliminating damaging crystal sample transfer.

9. 发明申请

WO2019240829A1 DEVICE AND SYSTEM FOR PRESERVING ANALYTES IN BLOOD SAMPLES DURING STORAGE AND TRANSPORTATION 审中-公开
公开(公告)号：WO2019240829A1
公开(公告)日：2019-12-19
申请号：PCT/US2018/045287
申请日：2018-08-03
申请人： VDI LABORATORY, LLC. , KEPLER DIAGNOSTICS, INC.
发明人： RINGOLD, Randy , RINGOLD, Tyson
IPC分类号： B01L3/00 , A61B5/15 , A61B5/157 , A61B10/00 , B01L3/06 , B01L3/14 , G01N1/28
摘要： Freshly obtained blood samples are deposited into a device that contains blood sample carriers, each of the carriers is designed to hold a fixed amount of blood and allow excess blood sample to flow through and be discarded. A desiccant within the blood sample carrier aids with the drying of the blood and the discarding of the excess sample blood. The device is stored/transported within a modified atmosphere package comprised of an impermeable sealable bag and an oxygen scavenger compound for removing oxygen from the bag.

10. 发明申请

WO2014016599A2 EVAPORATION OF SOLVENTS FROM SAMPLES 审中-公开
公开(公告)号：WO2014016599A2
公开(公告)日：2014-01-30
申请号：PCT/GB2013051982
申请日：2013-07-24
申请人： GENEVAC LTD
发明人： ROSE SYMON , DARRINGTON ROBERT HUGH DOUGLAS , POOLE JOHN MICHAEL , BROADBENT GRAHAM , LEE-SMITH DONALD ROGER , SMITH RICHARD JOHN
IPC分类号： B01L3/06 , B01L3/00
CPC分类号： B01L3/50825 , B01L3/06 , B01L3/5025 , B01L3/50853 , B01L9/00 , B01L2200/028 , B01L2200/0678 , B01L2200/0694 , B01L2300/047 , B01L2300/048 , B01L2300/10 , B01L2400/0409
摘要： Apparatus and methods are provided relating to evaporation of solvents from samples and to prolonging the time taken to carry out an evaporation procedure. A cap (2) for engaging with an open end (10) of a sample container (6) comprises an engaging surface for engaging with the open end, and a body portion (12) which extends over the open end. The body portion is preformed to define an opening (16) which provides a fluid pathway between the open end of the container and the surroundings of the container, such that the cap impedes vapour flow from a sample in the container to the surroundings of the container. A holder (4) for receiving a plurality of such caps is also provided and a method of evaporating a solvent from a sample in a sample container is described.

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