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1. 发明申请

WO2016105605A1 COMPREHENSIVE AND COMPARATIVE FLOW CYTOMETRY-BASED METHODS FOR IDENTIFYING THE STATE OF A BIOLOGICAL SYSTEM 审中-公开
标题翻译：用于识别生物系统状态的综合和比较流式细胞仪检测方法
公开(公告)号：WO2016105605A1
公开(公告)日：2016-06-30
申请号：PCT/US2015/046912
申请日：2015-08-26
申请人： ENZO BIOCHEM, INC. , RABBANI, Elazar , DONEGAN, James, J. , L'HULLIER, Andrew, Stewart
发明人： RABBANI, Elazar , DONEGAN, James, J. , L'HULLIER, Andrew, Stewart
IPC分类号： C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/6886 , C12Q1/6841 , C12Q1/708 , G01N33/505 , C12Q2525/301 , C12Q2545/101 , C12Q2545/113 , C12Q2565/101 , C12Q2565/1015 , C12Q2565/107 , C12Q2565/626
摘要： The present disclosure provides comprehensive and comparative flow cytometry-based methods for identifying the state of a biological system by identifying the phenotype of cells and correlating the phenotype with the gene expression profile of the cells, which is indicative of cell function. The cells can be immune cells from a subject suffering from immune -mediated disorders that result in imbalance of the immune system and impair a subject's ability to recognize self-antigens or fight infection or disease. In certain aspects, cell phenotype is identified by detecting and/or quantifying one or more markers on the cell surface or intracellularly, which readily enables identification of specific sub-types of cells of interest, which can then be analyzed for gene expression in order to assay cell function. In additional aspects, function of particular subsets of cells identified by cell surface markers is determined by detecting patterns of gene expression, expression of RNA or other markers, by detecting and/or quantifying transcription in the cell, by assaying DNA content, by assaying cell receptors, and/or by detecting the number and/or state of cellular organelles, receptors and/or transport systems.
摘要翻译：本公开提供了通过鉴定细胞表型并将表型与细胞的基因表达谱相关联来鉴定生物系统的状态的综合和比较的流式细胞术方法，其指示细胞功能。细胞可以是来自患有免疫介导的障碍的受试者的免疫细胞，其导致免疫系统的不平衡并且损害受试者识别自身抗原或抵抗感染或疾病的能力。在某些方面，通过检测和/或定量细胞表面或细胞内的一种或多种标志物来鉴定细胞表型，其可以容易地鉴定感兴趣的细胞的特定亚型，然后可以分析它们的基因表达，以便测定细胞功能。在另外的方面，通过检测基因表达，RNA或其他标志物的表达，通过检测和/或定量细胞中的转录，通过测定细胞DNA来测定DNA含量来确定特定细胞表面标记子集的功能和/或通过检测细胞器细胞器，受体和/或转运系统的数量和/或状态。

2. 发明申请

WO2009111072A1 MULTIPLEXED PCR-COUPLED PNA LABELED BEADS FLOW CYTOMETRIC ASSAY FOR SIMULTANEOUS DETECTION OF MULTIPLE BIOLOGICAL AGENTS 审中-公开
标题翻译：多重PCR-偶联PNA标记珠流动细胞因子测定同时检测多种生物学试剂
公开(公告)号：WO2009111072A1
公开(公告)日：2009-09-11
申请号：PCT/US2009/001466
申请日：2009-03-05
申请人： CLEAN EARTH TECHNOLOGIES, LLC , SU, Wan, Wen , GOLDEN, Jeffry
发明人： SU, Wan, Wen , GOLDEN, Jeffry
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q2565/626 , C12Q2537/143 , C12Q2525/107 , C12Q2563/173 , C12Q2563/155
摘要： A method for detection of multiple biological entities, such as biological warfare and terrorism agents, and public health threat pathogens, is accomplished by multiplexing PCR to amplify different DNA targets and to produce dsDNA products, then mixing the multiple PCR dsDNA products with beads of specific sizes that are labeled with specific complementary PNA labels and fluorescent staining dye so that the PNA-labeled beads bind with specific dsDNA by complementary sequences and fluorescent dsDNA dye combines with dsDNA, and then performing either flow cytometry to count beads by size and determine the intensity of fluorescence by size or performing size separation of two or more bead size fractions and detection by measuring the fluorescent signals of two or more bead size fractions.
摘要翻译：通过复用PCR扩增不同的DNA靶标并产生dsDNA产物，然后将多个PCR dsDNA产物与特异性的珠粒混合，从而实现了多种生物实体（如生物战和恐怖主义）以及公共卫生威胁病原体的检测方法。用特异性互补PNA标记和荧光染色染料标记的大小，使得PNA标记的珠粒通过互补序列与特异性dsDNA结合，并且荧光dsDNA染料与dsDNA结合，然后进行流式细胞术以通过大小计数珠粒并确定强度的荧光尺寸或进行两个或多个珠粒级分的尺寸分离，并通过测量两种或多种珠粒级分的荧光信号进行检测。

3. 发明申请

WO2009048878A3 MICROFLUIDIC PLATFORMS FOR MULTI-TARGET DETECTION 审中-公开
标题翻译：用于多目标检测的微流平台
公开(公告)号：WO2009048878A3
公开(公告)日：2009-06-04
申请号：PCT/US2008079094
申请日：2008-10-07
申请人： UNIV NOTRE DAME DU LAC , CHANG HSUEH-CHIA , GORDON JASON , SENAPATI SATYAJYOTI , GAGNON ZACHARY
发明人： CHANG HSUEH-CHIA , GORDON JASON , SENAPATI SATYAJYOTI , GAGNON ZACHARY
IPC分类号： C12M1/34
CPC分类号： B03C5/005 , B01J2219/00459 , B01J2219/005 , B01J2219/00511 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01L3/502761 , B03C5/026 , B03C2201/26 , C12Q1/6816 , G01N21/64 , C12Q2563/116 , C12Q2565/626 , C12Q2565/629
摘要： Disclosed are example methods and devices for detecting one or more targets. An example method includes placing a sample including a first target with in a microfiuidic device and hybridizing a plurality of copies of the first target with a plurality of nanostructures. The example method includes applying an electric current to the plurality of nanostructures and using an electric field created by the electric current to move the plurality of nanostructures. In addition, the plurality of nanostructures are sorted and evaluated to determine at least one of a presence, an absence, or a quantity of the first target.
摘要翻译：公开了用于检测一个或多个目标的示例性方法和装置。示例性方法包括将包含第一靶的样品放置在微流体装置中，并将多个第一靶的拷贝与多个纳米结构杂交。该示例性方法包括向多个纳米结构施加电流并且使用由电流产生的电场来移动多个纳米结构。此外，对多个纳米结构进行分类和评估以确定第一靶的存在，不存在或数量中的至少一个。

4. 发明申请

WO2009047756A2 METHODS AND KITS FOR DIAGNOSING LUNG CANCER 审中-公开
标题翻译：诊断肺癌的方法和试剂盒
公开(公告)号：WO2009047756A2
公开(公告)日：2009-04-16
申请号：PCT/IL2008001322
申请日：2008-10-06
申请人： BIOVIEW LTD , DANIELY MICHAL , MADI LEA , KAPLAN TAL , PAL BOAZ , HARARI YUVAL , ZAIDI TANWEER M , FERNANDEZ RICARDO L , ZHANG JINPING , KATZ RUTH
发明人： DANIELY MICHAL , MADI LEA , KAPLAN TAL , PAL BOAZ , HARARI YUVAL , ZAIDI TANWEER M , FERNANDEZ RICARDO L , ZHANG JINPING , KATZ RUTH
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6841 , C12Q2565/626 , C12Q2563/173
摘要： Provided is a method of identifying a genetically abnormal cell in a sputum sample, the method comprising: (a) staining a sputum sample using a morphological stain so as to identify a lower airway tract cell or lung cell in the sputum sample; and (b) staining the sputum sample using fluorescent in situ hybridization (FISH) so as to identify in the lower airway tract cell or lung cell a genetic abnormality in at least one of human chromosome 3p22.1 and 10q22-23, thereby identifying the genetically abnormal cell in the sputum sample. Also provided are methods and kits of diagnosing lung cancer by detecting a presence of genetically abnormal cells above a predetermined threshold in a sputum sample.
摘要翻译：本发明提供一种鉴别痰液样本中遗传异常细胞的方法，所述方法包括：（a）使用形态学染色剂对痰液样本进行染色以鉴定痰液样本中的下呼吸道细胞或肺细胞; （b）使用荧光原位杂交（FISH）染色痰样品，以便在下呼吸道细胞或肺细胞中鉴定人染色体3p22.1和10q22-23中的至少一个中的遗传异常，由此鉴定痰液样本中遗传异常的细胞。还提供了通过检测痰样品中高于预定阈值的遗传异常细胞的存在来诊断肺癌的方法和试剂盒。

5. 发明申请

WO2004042404A1 FRET PROBES AND METHODS FOR DETECTING INTERACTING MOLECULES 审中-公开
标题翻译：用于检测交互分子的FRET探针和方法
公开(公告)号：WO2004042404A1
公开(公告)日：2004-05-21
申请号：PCT/NL2003/000777
申请日：2003-11-06
申请人： ERASMUS UNIVERSITEIT ROTTERDAM , VAN DONGEN, Jacobus, Johannes, Maria , STAAL, Frank, Jakob, Theodor
发明人： VAN DONGEN, Jacobus, Johannes, Maria , STAAL, Frank, Jakob, Theodor
IPC分类号： G01N33/58
CPC分类号： B82Y5/00 , B82Y10/00 , C12Q1/6818 , G01N33/542 , G01N33/54333 , C12Q2565/626
摘要： This invention relates to the field of detecting interacting molecules. The invention provides a set of a first and a second probe each probe provided with a dye allowing energy transfer at least one probe provided with a reactive group allowing juxtaposing said first and second probe. A method is provided for detecting at least two interacting molecules at the single cell level using of a set of probes according to the invention.
摘要翻译：本发明涉及检测相互作用的分子的领域。本发明提供了一组第一和第二探针，每个探针设置有染料，其允许能量传递至少一个探针，其具有允许并置所述第一和第二探针的反应性基团。提供一种用于使用根据本发明的一组探针在单细胞水平上检测至少两个相互作用分子的方法。

6. 发明申请

WO2004018500A1 CODED NUCLEIC ACID CARRIERS 审中-公开
标题翻译：编码核酸载体
公开(公告)号：WO2004018500A1
公开(公告)日：2004-03-04
申请号：PCT/AU2003/001077
申请日：2003-08-22
申请人： GENERA BIOSYSTEMS PTY LTD , TOOHEY, Brendan, James , POETTER, Karl, Frederick
发明人： TOOHEY, Brendan, James , POETTER, Karl, Frederick
IPC分类号： C07K1/04
CPC分类号： C40B50/16 , B01J2219/00497 , B01J2219/005 , B01J2219/00504 , B01J2219/00511 , B01J2219/00572 , B01J2219/00576 , B01J2219/00581 , B01J2219/00585 , B01J2219/00596 , B01J2219/00599 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , C12N15/1068 , C12Q1/6834 , C12Q2600/156 , C40B40/06 , C40B40/10 , C40B40/12 , C12Q2565/626 , C12Q2563/179 , C12Q2563/167
摘要： The present invention relates generally to coded solid or semi-solid nucleic acid carriers for use in multiplexing solid phase nucleic acid-based reactions. The use of coded carriers facilitates multiplexing due to the ability to deconvolute multiple nucleic acid-based events and to correlate these to particular experiments. The present invention further provides a method for identifying a nucleic acid molecule having a defined characteristic within a population of two or more different nucleic acid molecules using coded nucleic acid carriers. Conversely, the nucleic acid can be used as the code for a particular peptide, or other chemical, bound specifically to a microsphere with a specific oligonucleotide sequence. Alternatively, the method of the present invention permits screening for molecules which interact with target nucleic acid, or other, molecules. The method and the coded carriers of the present invention enable high throughput screening of nucleic acid, or other, molecules. The method may also be automated and/or controlled by computer software.
摘要翻译：本发明一般涉及用于复用固相核酸反应的编码的固体或半固体核酸载体。编码载波的使用由于能够对多个基于核酸的事件进行解卷积并将其与特定实验相关联来促进多路复用。本发明还提供了使用编码的核酸载体鉴定具有在两个或多个不同核酸分子的群体内具有确定特征的核酸分子的方法。相反，核酸可以用作与具有特定寡核苷酸序列的微球特异性结合的特定肽或其它化学物的代码。或者，本发明的方法允许筛选与靶核酸或其它分子相互作用的分子。本发明的方法和编码载体能够实现核酸或其它分子的高通量筛选。该方法还可以由计算机软件自动化和/或控制。

7. 发明申请

WO02006524A2 A METHOD FOR DIRECT GENETIC ANALYSIS OF TARGET CELLS BY USING FLOURESCENCE PROBES 审中-公开
标题翻译：用荧光探针直接进行靶细胞遗传分析的方法
公开(公告)号：WO02006524A2
公开(公告)日：2002-01-24
申请号：PCT/EP2001/008202
申请日：2001-07-16
IPC分类号： G01N33/48 , C12N15/09 , C12Q1/02 , C12Q1/68 , G01N33/483 , G01N33/53 , G01N33/566
CPC分类号： C12Q1/6841 , C12Q2565/626
摘要： A method enables the identification of target cells from non-target cells comprising the step of in situ hybridising a target sequence in a target cell with a labelled sequence and cell identification of the target cell by the aid of detecting the hybridised labelled sequence by flow cytometry.
摘要翻译：本发明提供了一种用于从非靶细胞中鉴定靶细胞的方法，将靶细胞的靶序列与标记的序列原位杂交，并通过检测标记的序列来鉴定靶细胞。使用流式细胞仪进行杂交。

8. 发明申请

WO01094639A1 ADDRESS/CAPTURE TAGS FOR FLOW-CYTOMETRY BASED MINISEQUENCING 审中-公开
标题翻译：地址/捕获标签用于基于流式细胞仪的分类
公开(公告)号：WO01094639A1
公开(公告)日：2001-12-13
申请号：PCT/US2001/018590
申请日：2001-06-07
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人： WHITE, P., Scott , TORNEY, David, C.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6881 , C12Q1/6827 , C12Q1/6837 , C12Q2600/156 , C12Q2565/626 , C12Q2565/519 , C12Q2563/179 , C12Q2535/101
摘要： A method for generating address/capture tags for use in a sensitive and rapid flow-cytometry based assay for the multiplexed analysis of SNPs based on polymerase-mediated primer extension using microspheres as solid supports is described. Single-nucleotide polymorphisms (SNPs) are the most abundant type of human genetic variation. These variable sites are present at high density in the genome, making them powerful tools for mapping and diagnosing disease-related alleles. Subnanomolar concentrations of sample in small volumes (10 ml) can be analyzed at rates greater than one sample per minute, without a wash step. Genomic analysis using multiplexing microsphere arrays, enables the simultaneous analysis of dozens, and potentially hundreds of SNPs per sample. The method has been tested by genotyping the Glu69 variant from the HLA DPB1 locus, an SNP associated with chronic beryllium disease, as well as HLA DPA 1 alleles.
摘要翻译：描述了一种用于产生地址/捕获标签的方法，用于基于使用微球作为固体支持物的基于聚合酶介导的引物延伸的基于灵敏和快速流式细胞术的测定法，用于SNP的多重分析。单核苷酸多态性（SNP）是人类遗传变异最丰富的类型。这些可变位点在基因组中以高密度存在，使其成为绘制和诊断疾病相关等位基因的强大工具。少量样品（10ml）的亚纳摩尔浓度可以以大于每分钟一个样品的速率进行分析，无需洗涤步骤。使用复用微球阵列进行基因组分析，可以同时分析数十个，每个样本可能有数百个SNP。已经通过从HLA DPB1基因座，与慢性铍疾病相关的SNP以及HLA DPA 1等位基因对Glu69变体进行基因分型来测试该方法。

9. 发明申请

WO00039335A1 INDIVIDUALLY ADDRESSABLE SOLID SURFACES FOR MULTIPLEXED OPERATIONS 审中-公开
标题翻译：用于多重操作的单独寻址的固体表面
公开(公告)号：WO00039335A1
公开(公告)日：2000-07-06
申请号：PCT/US1999/029711
申请日：1999-12-15
IPC分类号： C12Q1/68 , G01N33/58
CPC分类号： B82Y15/00 , C12Q1/6827 , C12Q2600/156 , G01N33/582 , G01N33/583 , G01N33/588 , Y10T436/143333 , C12Q2565/626 , C12Q2563/149 , C12Q2563/107 , C12Q2537/143
摘要： Solid moieties are provided which are individually addressable by using individual or combinations of dyes, including at least one lanthanide dye. The solid moieties may be a bulk solid having individual sites or particles, where each site can provide a unique emission spectrum defining an entity bound to the site. The individually addressable moieties may be used in multiplexed determinations for determining the presence of biopolymers or other mixture of differentiable entities. Different protocols are provided for the determinations.
摘要翻译：提供了通过使用包括至少一种镧系染料的染料的单独或组合来单独寻址的固体部分。固体部分可以是具有单个位点或颗粒的体固体，其中每个位点可以提供限定结合到位点的实体的唯一发射光谱。单独可寻址的部分可以用于多重测定以确定生物聚合物或其他可微分实体的混合物的存在。为确定提供了不同的协议。

10. 发明申请

WO99022030A1 DNA POLYMORPHISM IDENTITY DETERMINATION USING FLOW CYTOMETRY 审中-公开
标题翻译： DNA多态性鉴定使用流式细胞术检测
公开(公告)号：WO99022030A1
公开(公告)日：1999-05-06
申请号：PCT/US1998/023143
申请日：1998-10-28
IPC分类号： C12N15/00 , C12N15/09 , C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6827 , C12Q1/6816 , C12Q1/6834 , C12Q1/6855 , C12Q1/6862 , C12Q1/6869 , C12Q2565/626 , C12Q2565/519 , C12Q2533/107 , C12Q2563/131 , C12Q2537/143 , C12Q2535/125 , C12Q2563/155
摘要： Primers designed to be immobilized on microspheres are allowed to anneal to the DNA strand under investigation, and are extended by either DNA polymerase using fluorescent dideoxynucleotides or ligated by DNA ligase to fluorescent reporter oligonucleotides. The fluorescence of either the dideoxynucleotide or the reporter oligonucleotide attached to the immobilized primer is measured by flow cytometry, thereby identifying the nucleotide polymorphism on the DNA strand.
摘要翻译：被设计为固定在微球上的引物被允许与被研究的DNA链退火，并通过DNA聚合酶使用荧光双脱氧核苷酸延伸或通过DNA连接酶与荧光报道寡核苷酸连接。通过流式细胞术测定与固定化引物连接的双脱氧核苷酸或报告寡核苷酸的荧光，从而鉴定DNA链上的核苷酸多态性。

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