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1. 发明申请

WO2016105605A1 COMPREHENSIVE AND COMPARATIVE FLOW CYTOMETRY-BASED METHODS FOR IDENTIFYING THE STATE OF A BIOLOGICAL SYSTEM 审中-公开
标题翻译：用于识别生物系统状态的综合和比较流式细胞仪检测方法
公开(公告)号：WO2016105605A1
公开(公告)日：2016-06-30
申请号：PCT/US2015/046912
申请日：2015-08-26
申请人： ENZO BIOCHEM, INC. , RABBANI, Elazar , DONEGAN, James, J. , L'HULLIER, Andrew, Stewart
发明人： RABBANI, Elazar , DONEGAN, James, J. , L'HULLIER, Andrew, Stewart
IPC分类号： C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/6886 , C12Q1/6841 , C12Q1/708 , G01N33/505 , C12Q2525/301 , C12Q2545/101 , C12Q2545/113 , C12Q2565/101 , C12Q2565/1015 , C12Q2565/107 , C12Q2565/626
摘要： The present disclosure provides comprehensive and comparative flow cytometry-based methods for identifying the state of a biological system by identifying the phenotype of cells and correlating the phenotype with the gene expression profile of the cells, which is indicative of cell function. The cells can be immune cells from a subject suffering from immune -mediated disorders that result in imbalance of the immune system and impair a subject's ability to recognize self-antigens or fight infection or disease. In certain aspects, cell phenotype is identified by detecting and/or quantifying one or more markers on the cell surface or intracellularly, which readily enables identification of specific sub-types of cells of interest, which can then be analyzed for gene expression in order to assay cell function. In additional aspects, function of particular subsets of cells identified by cell surface markers is determined by detecting patterns of gene expression, expression of RNA or other markers, by detecting and/or quantifying transcription in the cell, by assaying DNA content, by assaying cell receptors, and/or by detecting the number and/or state of cellular organelles, receptors and/or transport systems.
摘要翻译：本公开提供了通过鉴定细胞表型并将表型与细胞的基因表达谱相关联来鉴定生物系统的状态的综合和比较的流式细胞术方法，其指示细胞功能。细胞可以是来自患有免疫介导的障碍的受试者的免疫细胞，其导致免疫系统的不平衡并且损害受试者识别自身抗原或抵抗感染或疾病的能力。在某些方面，通过检测和/或定量细胞表面或细胞内的一种或多种标志物来鉴定细胞表型，其可以容易地鉴定感兴趣的细胞的特定亚型，然后可以分析它们的基因表达，以便测定细胞功能。在另外的方面，通过检测基因表达，RNA或其他标志物的表达，通过检测和/或定量细胞中的转录，通过测定细胞DNA来测定DNA含量来确定特定细胞表面标记子集的功能和/或通过检测细胞器细胞器，受体和/或转运系统的数量和/或状态。

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