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1. 发明申请

WO2004042404A1 FRET PROBES AND METHODS FOR DETECTING INTERACTING MOLECULES 审中-公开
标题翻译：用于检测交互分子的FRET探针和方法
公开(公告)号：WO2004042404A1
公开(公告)日：2004-05-21
申请号：PCT/NL2003/000777
申请日：2003-11-06
申请人： ERASMUS UNIVERSITEIT ROTTERDAM , VAN DONGEN, Jacobus, Johannes, Maria , STAAL, Frank, Jakob, Theodor
发明人： VAN DONGEN, Jacobus, Johannes, Maria , STAAL, Frank, Jakob, Theodor
IPC分类号： G01N33/58
CPC分类号： B82Y5/00 , B82Y10/00 , C12Q1/6818 , G01N33/542 , G01N33/54333 , C12Q2565/626
摘要： This invention relates to the field of detecting interacting molecules. The invention provides a set of a first and a second probe each probe provided with a dye allowing energy transfer at least one probe provided with a reactive group allowing juxtaposing said first and second probe. A method is provided for detecting at least two interacting molecules at the single cell level using of a set of probes according to the invention.
摘要翻译：本发明涉及检测相互作用的分子的领域。本发明提供了一组第一和第二探针，每个探针设置有染料，其允许能量传递至少一个探针，其具有允许并置所述第一和第二探针的反应性基团。提供一种用于使用根据本发明的一组探针在单细胞水平上检测至少两个相互作用分子的方法。

2. 发明申请

WO2005015235A1 METHOD FOR DETECTING LOW LEVELS OF A FUSION PROTEIN 审中-公开
标题翻译：检测融合蛋白低水平的方法
公开(公告)号：WO2005015235A1
公开(公告)日：2005-02-17
申请号：PCT/NL2004/000562
申请日：2004-08-11
申请人： ERASMUS UNIVERSITY MEDICAL CENTER ROTTERDAM , STAAL, Frank, Jakob, Theodor , VAN DONGEN, Jacobus, Johannes, Maria
发明人： STAAL, Frank, Jakob, Theodor , VAN DONGEN, Jacobus, Johannes, Maria
IPC分类号： G01N33/574
CPC分类号： G01N33/57426 , G01N33/54306 , G01N33/57484
摘要： The invention relates to the detection of among others tumor-specific fusion proteins. Provided is a method for detecting a fusion protein in a sample, said fusion protein comprising an amino-terminal fragment and a carboxy-terminal fragment which are each corresponding to a native protein, said method comprising contacting said sample with at least one binding molecule specifically reactive with a part of the native protein that is not present in the fusion protein, under conditions that allow for the formation of a complex between said at least one binding molecule and said native protein, removing said complex from said sample to deplete said sample of said native protein but not of said fusion protein; and detecting said fusion protein in said sample using at least one antibody probe directed against said fusion protein. Also provided is a diagnostic kit for carrying out a method of the invention.
摘要翻译：本发明涉及其他肿瘤特异性融合蛋白的检测。提供了一种用于检测样品中融合蛋白的方法，所述融合蛋白包含氨基末端片段和羧基末端片段，其各自对应于天然蛋白质，所述方法包括使所述样品与至少一种结合分子特异性地接触在允许在所述至少一个结合分子和所述天然蛋白质之间形成复合物的条件下，与所述融合蛋白中不存在的天然蛋白质的一部分反应，从所述样品中除去所述复合物以将所述样品去除所述天然蛋白质，但不是所述融合蛋白质; 以及使用针对所述融合蛋白的至少一个抗体探针检测所述样品中的所述融合蛋白。还提供了用于实施本发明的方法的诊断试剂盒。

3. 发明申请

WO2004042398A1 METHOD AND PROBES FOR THE DETECTION OF A TUNOR SPECIFIC FUSION PROTEIN 审中-公开
标题翻译：检测特异性融合蛋白的方法和探针
公开(公告)号：WO2004042398A1
公开(公告)日：2004-05-21
申请号：PCT/NL2003/000776
申请日：2003-11-06
申请人： ERASMUS UNIVERSITEIT ROTTERDAM , VAN DONGEN, Jacobus, Johannes, Maria
发明人： VAN DONGEN, Jacobus, Johannes, Maria
IPC分类号： G01N33/542
CPC分类号： B82Y20/00 , B82Y5/00 , B82Y10/00 , G01N33/542 , G01N33/574 , G01N33/57426 , G01N33/57496 , Y10T436/108331
摘要： This invention relates to the detection of fusion proteins. The invention provides a set of at least a first and a second molecular probe, each probe provided with a dye wherein said dyes together allow energy transfer; at least one probe provided with a reactive group allowing juxtaposing said at least first and second probe wherein said reactive group allows to modulate juxtaposing said probes such that there is an increased likelihood of energy transfer between said dyes. A method is provided which permits detecting the presence of a fusion protein in a cell at the single cell level.
摘要翻译：本发明涉及融合蛋白的检测。本发明提供一组至少第一和第二分子探针，每个探针提供有染料，其中所述染料一起允许能量转移; 提供有反应性基团的至少一个探针允许并置所述至少第一和第二探针，其中所述反应性基团允许调节所述探针并置，使得所述染料之间能量转移的可能性增加。提供了一种允许在单细胞水平检测细胞中融合蛋白的存在的方法。

4. 发明申请

WO2007123392A1 DETERMINING THE REPLICATIVE HISTORY OF LYMPHOCYTES 审中-公开
标题翻译：确定LYMPHOCYTES的替代历史
公开(公告)号：WO2007123392A1
公开(公告)日：2007-11-01
申请号：PCT/NL2006/000219
申请日：2006-04-24
申请人： Erasmus Universiteit Rotterdam , VAN DONGEN, Jacobus, Johannes, Maria , SZCZEPANSKI, Thomasz
发明人： VAN DONGEN, Jacobus, Johannes, Maria , SZCZEPANSKI, Thomasz
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876
摘要： The invention relates to the field of immunology and immunodiagnostics. Provided are primers and probes suitable for use in a method for determining the replicative history of a lymphocyte, preferably a B cell, said method comprising detecting a signal joint nucleotide sequence on an extrachromosomal circular excision product in said lymphocyte, wherein said excision product is deleted from a chromosome to give a chromosomal coding joint nucleotide sequence, wherein said coding joint is retained in the chromosome, and detecting said coding joint nucleotide sequence in said lymphocyte. The primers and probes provided herein is among others advantageously used to assess recovery of the precursor B cell compartment, for example in a patient following bone marrow transplantation
摘要翻译：本发明涉及免疫学和免疫诊断领域。提供适用于确定淋巴细胞（优选B细胞）的复制史的方法的引物和探针，所述方法包括检测所述淋巴细胞中染色体外循环切除产物上的信号联合核苷酸序列，其中所述切除产物被缺失从染色体得到染色体编码关节核苷酸序列，其中所述编码关节保留在染色体中，并检测所述淋巴细胞中的所述编码关节核苷酸序列。本文提供的引物和探针有利于用于评估前体B细胞隔室的回收，例如在骨髓移植后的患者中

5. 发明申请

WO2004033728A2 NUCLEIC ACID AMPLIFICATION PRIMERS FOR PCR-BASED CLONALITY STUDIES 审中-公开
标题翻译：用于基于PCR的克隆研究的核酸扩增引物
公开(公告)号：WO2004033728A2
公开(公告)日：2004-04-22
申请号：PCT/NL2003/000690
申请日：2003-10-13
申请人： ERASMUS UNIVERSITEIT ROTTERDAM , DAVI, Frederic, Bernard, Louis , VAN DONGEN, Jacobus, Johannes, Maria , LANGERAK, Anthonie, Willem , SCHUURING, Eduardus, Maria, Dominicus , SAN MIQUEL, Jesus, Fernando , GARZIA SANZ, Ramon , PARREIRA, Antonio , SMITH, John, Lewis , LAVENDER, Frances, Louise , MORGAN, Gareth, John , EVANS, Paul, Anthony, Stuart , KNEBA, Michael , HUMMEL, Michael , MACINTYRE, Elizabeth, Anne , BASTARD, Christian
发明人： VAN DONGEN, Jacobus, Johannes, Maria , LANGERAK, Anthonie, Willem , SCHUURING, Eduardus, Maria, Dominicus , SAN MIQUEL, Jesus, Fernando , GARZIA SANZ, Ramon , PARREIRA, Antonio , SMITH, John, Lewis , LAVENDER, Frances, Louise , MORGAN, Gareth, John , EVANS, Paul, Anthony, Stuart , KNEBA, Michael , HUMMEL, Michael , MACINTYRE, Elizabeth, Anne , BASTARD, Christian
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q1/6886 , C12Q2600/112 , C12Q2600/156 , C12Q2600/16
摘要： The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a rearrangement selected from the group consisting of a VH-JH IGH rearrangement, a DH-JH IGH rearrangement, a VK-JK IGK rearrangement, a VK/intron-Kde IGK rearrangement, a Vλ-Jλ IGL rearrangement, a Vß-Jß TCRB rearrangement, a Dß-Jß TCRB rearrangement, a Vγ-Jγ TCRG rearrangement, a Vδ-Jδ TCRD rearrangement, a Dδ-Dδ TCRD rearrangement, a Dδ-Jδ TCRD rearrangement, a Vδ-Dδ TCRD rearrangement, or a translocation selected from t(11;14)(BCL1-IGH) and t(14;18)(BCL2-IGH). The primers can be used in PCR-based clonality studies for early diagnosis of lymphoproliferative disorders and detection of minimal residual disease (MRD). Also provided is a kit comprising at least one set of primers of the invention.
摘要翻译：本发明涉及基于PCR的克隆性研究，用于淋巴组织增生性疾病的早期诊断等。提供一组核酸扩增引物，其包含能够扩增选自以下的重排的正向引物或其变体和反向引物或其变体：VH-JH IGH重排，DH- JH IGH重排，VK-JK IGK重排，VK /内含子-Kde IGK重排，Vλ-JλIGL重排，V＆amp; Szlig; -J＆amp; Szlig; TCRB重排，D＆Szig; -J＆Szlig; TCRB重排，Vγ-JγTCRG重排，Vδ-JδTCRD重排，Dδ-DδTCRD重排，Dδ-JδTCRD重排，Vδ-DδTCRD重排或选自t（11; 14）（ BCL1-IGH）和t（14; 18）（BCL2-IGH）。该引物可用于基于PCR的克隆性研究，用于早期诊断淋巴组织增生性疾病和检测微小残留病（MRD）。还提供了包含至少一组本发明引物的试剂盒。

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