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1. 发明申请

WO2018093976A1 ANALYSIS OF NUCLEIC ACIDS USING PROBE WITH NON-LINEAR TAG 审中-公开
公开(公告)号：WO2018093976A1
公开(公告)日：2018-05-24
申请号：PCT/US2017/061905
申请日：2017-11-16
申请人： NANOPORE DIAGNOSTICS, LLC
发明人： DAS, Somes, K. , COHEN, Thomas, L. , REGELIN, Julie
IPC分类号： C12Q1/6825 , C12Q1/6816 , C12Q1/6818
CPC分类号： C12Q1/6825 , C12Q1/6816 , C12Q1/6818 , C12Q2563/155 , C12Q2565/102 , C12Q2565/631 , C12Q2563/167 , C12Q2525/161 , C12Q2563/179 , C12Q2563/161
摘要： Probe molecules useful for detection of target nucleic acids using a nanopore system are described. The probe molecules may include at least one tag, e.g., combinations of multiple tags, to provide for unique signature current patterns that allow for identification of multiple target nucleic acids. The method of detecting a target nucleic acid may include combining a sample with at least one probe molecule having a sequence fully complementary or partially complementary to the target nucleic acid, such that the probe molecule hybridizes to the target nucleic acid. The sample may be added to a chamber of a nanopore system and a voltage applied to generate a current time series, wherein signature current patterns of the nanopore system indicate the presence difference target nucleic acids in the sample.

2. 发明申请

WO2017201060A1 ENHANCED FLUORESCENCE READOUT AND REDUCED INHIBITION FOR NUCLEIC ACID AMPLIFICATION TESTS 审中-公开
标题翻译：增强荧光读数和减少核酸扩增测试的抑制
公开(公告)号：WO2017201060A1
公开(公告)日：2017-11-23
申请号：PCT/US2017/032922
申请日：2017-05-16
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人： DI CARLO, Dino , KONG, Janay , OZCAN, Aydogan , GARNER, Omai
IPC分类号： C12Q1/68 , C12N9/12 , C09B23/04 , C09B23/00
CPC分类号： C12Q1/6844 , C09B15/00 , C09B23/04 , C09B29/16 , C09B67/0041 , C09B69/045 , C09B69/06 , C12N9/12 , C12Q1/68 , C12Q1/6818 , C12Q2521/513 , C12Q2563/173
摘要： A fluorescent dye and quencher mixture for reporting on nucleic acid amplification from a sample includes a fluorescent intercalating dye, a dye sequestering or quenching agent such as hydroxynapthol blue (HNB) or caffeine, and primers, dNTPs, and a nucleic acid polymerizing enzyme or fragment thereof. The presence of the dye in combination with the dye sequestering or quenching agent improves the overall dynamic range of the fluorescent signal as well as shortens the time needed for visualization or image capture of amplified nucleic acid. The fluorescent dye and quencher mixture also enables the detection of nucleic acids in samples having low copy numbers.
摘要翻译：用于报告来自样品的核酸扩增的荧光染料和猝灭剂混合物包括荧光插入染料，染料螯合剂或猝灭剂如羟基萘酚蓝（HNB）或咖啡因，以及引物，dNTP，和核酸聚合酶或其片段。染料与染料螯合剂或猝灭剂的存在改善了荧光信号的整体动态范围，并缩短了扩增核酸的可视化或图像捕获所需的时间。荧光染料和猝灭剂混合物还能够检测低拷贝数样品中的核酸。

3. 发明申请

WO2017009588A1 METHODE DE PRONOSTIC DE PATHOLOGIES 审中-公开
标题翻译：病理学预防方法
公开(公告)号：WO2017009588A1
公开(公告)日：2017-01-19
申请号：PCT/FR2016/051838
申请日：2016-07-18
申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE , UNIVERSITE DE MONTPELLIER , AXLR, SATT DU LANGUEDOC ROUSSILLON , INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE (INSERM)
发明人： BIENVENU, Frédéric
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/6818 , C12Q1/6883 , C12Q2600/106 , C12Q2600/118 , C12Q2600/156 , C12Q2600/158 , C12Q2600/166 , C12Q2600/178
摘要： L'invention concerne une méthode de pronostic de la susceptibilité à un stress environnemental d'un individu, la méthode comprenant une étape de détermination de la séquence d'initiation de la traduction de l'un au moins des gènes de la famille Ccnd, et une étape de quantification de l'expression de la protéine codée par ledit gène dont la séquence d'initiation de la traduction a été séquencée à l'étape précédente, et la comparaison avec le niveau d'expression de ladite protéine issue d'un échantillon de référence.
摘要翻译：本发明涉及一种人对环境压力易感性的预测方法。该方法包括：确定用于启动来自CCND家族的至少一个基因的翻译的序列的步骤，以及定量由所述基因编码的蛋白质的表达的步骤，其翻译起始顺序在并将所述表达与所述蛋白质与参照样品的表达水平进行比较。

4. 发明申请

WO2016178953A1 MULTIPLEX ANALYSIS OF GENE EXPRESSION IN INDIVIDUAL LIVING CELLS 审中-公开
标题翻译：基因表达在个体生活细胞中的多重分析
公开(公告)号：WO2016178953A1
公开(公告)日：2016-11-10
申请号：PCT/US2016/029972
申请日：2016-04-29
申请人： THE GENERAL HOSPITAL CORPORATION
发明人： ATMANLI, Ayhan , DOMIAN, Ibrahim J.
IPC分类号： C12Q1/68 , C07K14/00 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6818 , C07H21/02 , C07H21/04 , C12N15/1086 , C12N15/70 , C12Q1/6834 , C12Q1/6841 , C12Q2522/101 , C12Q2563/107 , C12Q2563/119 , G01N33/58 , C12Q2565/101
摘要： The technology as disclosed herein relates to methods, compostions and kits for multiplex measuring levels of expression of target RNA species (e.g., mRNA and non-coding RNAs) in single, living cells. Aspects of the invention relate to, in part, a duplex-binding protein which is labeled with a FRET dye, and a RNA-binding probe, which comprises a spectrally paired FRET dye and specifically hybridizes to a target RNA. When the RNA-binding probe binds to a target RNA, a duplex is formed, which is allows binding of the duplex-binding protein bringing the two FRET dyes into close proximity and allowing fluorescence resonance energy transfer (FRET) reaction and a detectable change in fluorescence, which determines the amount of target RNA species in the living cell. Apsects of the invention also include, kits, vectors and polynucleic acid sequences of the duplex-binding protein and RNA-binding probes disclosed herein, and cell and cell lines comprising the same.
摘要翻译：本文公开的技术涉及用于多重测量单个活细胞中靶RNA种类（例如mRNA和非编码RNA）的表达水平的方法，组合物和试剂盒。本发明的方面部分涉及用FRET染料标记的双链体结合蛋白和包含光谱配对FRET染料并与靶RNA特异性杂交的RNA结合探针。当RNA结合探针与靶RNA结合时，形成双链体，其允许双链体结合蛋白的结合使两种FRET染料紧密接近，并允许荧光共振能量转移（FRET）反应和可检测的变化荧光，其确定活细胞中靶RNA种类的量。本发明的嵌段还包括本文公开的双链体结合蛋白和RNA结合探针的试剂盒，载体和多核酸序列，以及包含其的细胞和细胞系。

5. 发明申请

WO2016123243A1 METHODS AND COMPOSITIONS FOR LABELING A SINGLE-STRANDED TARGET NUCLEIC ACID 审中-公开
标题翻译：用于标记单条目标核酸的方法和组合物
公开(公告)号：WO2016123243A1
公开(公告)日：2016-08-04
申请号：PCT/US2016/015178
申请日：2016-01-27
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人： O'CONNELL, Mitchell R. , DOUDNA, Jennifer A.
IPC分类号： C12N15/00 , C12N9/14 , C12N9/22 , C12Q1/68
CPC分类号： C12Q1/6806 , C12N9/22 , C12Q1/6818 , C12Q1/701
摘要： The present disclosure provides compositions and methods for labeling a single stranded target nucleic acid. Subject compositions include a Cas9 protein, a Cas9 guide RNA, and a quenched PAMmer. A subject quenched PAMmer is a single stranded oligonucleotide having (i) a protospacer adjacent motif (PAM) sequence; (ii) a detectable label; (iii) a quencher moiety that quenches the detectable label; and (iv) at least one of: a specificity segment positioned 5 of the PAM sequence, and an orientation segment positioned 3 of the PAM sequence. In the subject methods, the Cas9 protein cleaves the quenched PAMmer at a cleavage site positioned between the detectable label and the quencher moiety to produce: (a) a first cleavage product that is hybridized with the target nucleic acid and comprises the detectable label; and (b) a second cleavage product that is not hybridized with the target nucleic acid and comprises the quencher moiety.
摘要翻译：本公开提供了用于标记单链靶核酸的组合物和方法。主题组合物包括Cas9蛋白，Cas9引导RNA和淬灭的PAMmer。猝灭的PAMmer是单链寡核苷酸，其具有（i）原始邻近基序（PAM）序列; （ii）可检测标签; （iii）淬灭可检测标记的猝灭剂部分; 和（iv）至少一个：位于PAM序列5的特异性区段和位于PAM序列3的定向区段。在本发明方法中，Cas9蛋白在位于可检测标记和猝灭剂部分之间的切割位点处切割淬灭的PAMmer，以产生：（a）与靶核酸杂交并包含可检测标记的第一切割产物; 和（b）第二切割产物，其不与靶核酸杂交并且包含猝灭剂部分。

6. 发明申请

WO2016097952A1 METHOD FOR THE COLORIMETRIC DETECTION OF CONTAMINATION WITH NUCLEASES 审中-公开
标题翻译：用核对颜料检测污染的方法
公开(公告)号：WO2016097952A1
公开(公告)日：2016-06-23
申请号：PCT/IB2015/059541
申请日：2015-12-11
申请人： FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA
发明人： POMPA, Pier Paolo , VALENTINI, Paola , CECERE, Paola
IPC分类号： C12Q1/34 , C12Q1/68
CPC分类号： C12Q1/34 , C12Q1/6818 , C12Q2521/301 , C12Q2521/307 , C12Q2521/319 , C12Q2521/327 , C12Q2563/137 , C12Q2563/155 , C12Q2565/113 , G01N2333/916 , G01N2333/922
摘要： Method for detecting the presence of nucleases in a sample, characterized in that it comprises the steps of: - incubating the sample to be tested for the presence of nucleases with at least one oligonucleotide linker constituting the substrate for the nuclease to be detected, for a sufficient time to cause degradation of said oligonucleotide linker by the nuclease possibly present in the sample, - adding to the sample, upon incubation, colloidal gold nanoparticles comprising gold nanoparticles functionalized with a first probe oligonucleotide and gold nanoparticles functionalized with a respective second probe oligonucleotide, said first and second probe oligonucleotides being complementary to a respective portion of the nucleotide sequence of the oligonucleotide linker, and - examining the possible colour change of the sample as a result of the addition of said nanoparticles, a colour change of the sample to the colour assumed by the colloidal gold particles when aggregated at a distance less than their size being indicative of the absence of the tested nuclease from the sample.
摘要翻译：用于检测样品中核酸酶的存在的方法，其特征在于其包括以下步骤： - 将待测试的样品与至少一个构成要检测的核酸酶的底物的寡核苷酸接头的核酸酶的存在孵育，足够的时间引起可能存在于样品中的核酸酶引起的所述寡核苷酸接头的降解， - 加入到样品中，温育后，包含用第一探针寡核苷酸官能化的金纳米颗粒和用相应的第二探针寡核苷酸官能化的金纳米颗粒的胶体金纳米颗粒，所述第一和第二探针寡核苷酸与寡核苷酸接头的核苷酸序列的相应部分互补，并且 - 检查作为添加所述纳米颗粒的结果可能的样品颜色变化，样品的颜色变化为颜色由胶体金颗粒假定在分散时聚集小于它们的大小指示样品中没有测试的核酸酶。

7. 发明申请

WO2016085036A1 PROBE SYSTEM FOR REAL-TIME QUANTITATIVE AND QUALITATIVE ANALYSIS OF BIOMATERIAL, REACTION CHAMBER WITH SAID PROBE SYSTEM, AND ANALYSIS METHOD THEREOF 审中-公开
标题翻译：用于实时定量和定性分析生物体的反应体系，具有所述探针系统的反应室及其分析方法
公开(公告)号：WO2016085036A1
公开(公告)日：2016-06-02
申请号：PCT/KR2015/000817
申请日：2015-01-26
申请人： K-MAC
发明人： KIM, Lee Kyung , PAEK, Mun Cheol , KU, Su Jin , PARK, Sun Young , PARK, Jong Pil , LEE, Do Bu , KIM, Nam Joong , NOH, Jin Seok
IPC分类号： C12Q1/68 , G01N33/50 , C12M1/34 , G01N21/64
CPC分类号： C12Q1/6837 , C12Q1/6818 , C12Q1/6823 , C12Q1/6825 , C12Q1/6834 , G01N2021/6432 , C12Q2525/161 , C12Q2525/301 , C12Q2537/149 , C12Q2561/109 , C12Q2565/1015
摘要： A probe system for real-time quantitative and qualitative analysis of a biomaterial, and a reaction chamber with the probe system, and an analysis method thereof are provided. The probe system, which is included in the reaction chamber having an optically transmissive flat bottom surface and having a test sample accommodated therein, includes a target probe-reporter probe linker accommodated in the reaction chamber and including a target probe, which includes a sequence complementary to a target nucleic acid sequence to be detected, a first fluorophore and a first quencher, and a reporter probe linked to an end of the target probe and including a sequence non-complementary to the target nucleic acid sequence, and a capture probe included in a biochip formed on a bottom surface of the reaction chamber and including a complementary sequence hybridizable with the non-complementary sequence of the reporter probe, a second fluorophore and a second quencher.
摘要翻译：提供了一种用于生物材料的实时定量和定性分析的探针系统，以及具有探针系统的反应室及其分析方法。包含在具有光学透射平坦的底部表面并且具有容纳在其中的测试样品的反应室中的探针系统包括容纳在反应室中的目标探针 - 报告物探针连接体，并包括目标探针，其包括序列互补至待检测的靶核酸序列，第一荧光团和第一猝灭剂，以及与靶标探针的末端连接并包含与靶核酸序列不互补的序列的报告基因探针，以及包含在靶核酸序列中的捕获探针生物芯片形成在反应室的底表面上并且包括可与报道探针的非互补序列杂交的互补序列，第二荧光团和第二猝灭剂。

8. 发明申请

WO2016022526A1 RUTHENIUM-BASED PHOTOLINKERS AND METHODS OF USE 审中-公开
标题翻译：基于红宝石的光催化剂及其使用方法
公开(公告)号：WO2016022526A1
公开(公告)日：2016-02-11
申请号：PCT/US2015/043548
申请日：2015-08-04
申请人： THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
发明人： DMOCHOWSKI, Ivan J. , GRIEPENBURG, Julianne C. , RAPP, Teresa L.
IPC分类号： B01J31/18
CPC分类号： C07F15/0053 , B01J31/1815 , B01J35/004 , B01J2531/821 , C07H23/00 , C12N13/00 , C12N15/113 , C12N2310/11 , C12N2310/111 , C12N2310/32 , C12N2310/3233 , C12N2310/351 , C12N2310/3517 , C12N2310/531 , C12Q1/6818 , C12Q1/6823 , C12Q1/6825
摘要： The present invention provides ruthenium-based photolinker compounds, caged molecules comprising the ruthenium-based photolinker compounds, and methods of use. In certain aspects, the compositions disclosed herein comprise an active domain conjugated to a ruthenium-based photolinker, such that irradiation of the photolinker exposes the active domain. The present invention includes a composition comprising at least one ruthenium-based photolinker compound of formula (II), wherein in formula (II): L1, L2, L3, and L4 are each independently a ligand; and X1 and X2 are each independently a photolabile ligand having a reactive moiety.
摘要翻译：本发明提供了钌基光接枝剂化合物，包含钌基光接枝剂化合物的笼状分子和使用方法。在某些方面，本文公开的组合物包含与钌基光引发剂缀合的活性结构域，使得光引发剂的照射暴露于活性域。本发明包括包含至少一种式（II）的钌基光接枝化合物的组合物，其中在式（II）中：L1，L2，L3和L4各自独立地为配体; X1和X2各自独立地为具有反应性部分的光不稳定配体。

9. 发明申请

WO2015160726A3 A GENERALIZABLE ASSAY FOR VIRUS CAPSID ASSEMBLY 审中-公开
标题翻译：用于病毒组件的通用测定
公开(公告)号：WO2015160726A3
公开(公告)日：2015-12-10
申请号：PCT/US2015025632
申请日：2015-04-14
申请人： UNIV INDIANA RES & TECH CORP
发明人： ZLOTNICK ADAM , ANIAGYEI STELLA
IPC分类号： C12Q1/68
CPC分类号： C12Q1/703 , C12Q1/6818 , C12Q1/70 , C12Q2600/136
摘要： Virus capsids protect the viral genome and play roles in its delivery and intracellular transport, making them an attractive target for antiviral therapeutics. The difficulty in targetting capsid assembly is to identify molecules that interfere with the weak protein-protein interactions that drive the reaction. We have developed an in vitro assay for capsid assembly that works on a range of viruses at biologically relevant protein concentrations to facilitate screening large libraries of chemicals for lead compounds.
摘要翻译：病毒衣壳保护病毒基因组并在其递送和细胞内运输中发挥作用，使其成为抗病毒治疗的有吸引力的靶标。靶向衣壳装配的困难是确定干扰促进反应的弱蛋白质 - 蛋白质相互作用的分子。我们开发了一种用于衣壳组装的体外测定法，其用于生物相关蛋白浓度的一系列病毒，以便筛选铅化合物的大型化学物质文库。

10. 发明申请

WO2015172134A1 COSMIC QUENCHERS 审中-公开
公开(公告)号：WO2015172134A1
公开(公告)日：2015-11-12
申请号：PCT/US2015/030097
申请日：2015-05-11
申请人： BIOSEARCH TECHNOLOGIES, INC.
发明人： REDDINGTON, Mark , COOK, Ronald, M. , SOWERS, Ben
IPC分类号： C07D221/02 , C07D221/06
CPC分类号： C07D471/06 , C07D455/04 , C07F9/6561 , C07H17/02 , C07H21/00 , C12Q1/6818
摘要： The present invention provides quenchers of excited state energy, probes and other conjugates comprising these quenchers, and methods of their use.
摘要翻译：本发明提供了包含这些猝灭剂的激发态能量猝灭剂，探针和其它共轭物及其使用方法。

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