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1. 发明申请

US20130344540A1 METHODS FOR MINIMIZING SEQUENCE SPECIFIC BIAS 审中-公开
标题翻译：最小化序列特定偏差的方法
公开(公告)号：US20130344540A1
公开(公告)日：2013-12-26
申请号：US13980302
申请日：2012-01-10
申请人： Mark T. Reed , Mark Pratt
发明人： Mark T. Reed , Mark Pratt
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12Q1/6837 , C12Q1/6846 , C12Q2565/501
摘要： Provided herein are methods of nucleic acid amplification. An exemplary method includes the steps of (a) providing a surface comprising a plurality of patches of primers, (b) providing a plurality of different nucleic acid molecules, (c) contacting the plurality of different nucleic acid molecules with the surface under conditions wherein the nucleic acid molecules bind to primers at only a subset of the patches, (d) amplifying the nucleic acid molecules under conditions to saturate the subset of patches with copies of the nucleic acid molecules, and repeating steps (c) and (d), thereby increasing the number of patches that are saturated with copies of the nucleic acid molecules.
摘要翻译：本文提供了核酸扩增的方法。示例性方法包括以下步骤：（a）提供包含多个引物片的表面，（b）提供多个不同的核酸分子，（c）在多个不同的核酸分子的条件下使多个不同的核酸分子与表面接触，所述核酸分子仅在片段的一个子集上结合引物，（d）在使所述核酸分子拷贝饱和的条件下扩增核酸分子，并重复步骤（c）和（d），从而增加用核酸分子拷贝饱和的斑块的数量。

2. 发明授权

US08951781B2 Systems, methods, and apparatuses to image a sample for biological or chemical analysis 有权
标题翻译：用于对样品进行成像以进行生物或化学分析的系统，方法和装置
公开(公告)号：US08951781B2
公开(公告)日：2015-02-10
申请号：US13273666
申请日：2011-10-14
申请人： Mark T. Reed , Eric Williamson , Bryan Crane , Patrick Leung , Dale Buermann , Alexander P. Kindwall , Frederick Erie , Mark Pratt , Jason Harris , Andrew James Carson , Stanley S. Hong , Jason Bryant , Mark Wang , Drew Verkade
发明人： Mark T. Reed , Eric Williamson , Bryan Crane , Patrick Leung , Dale Buermann , Alexander P. Kindwall , Frederick Erie , Mark Pratt , Jason Harris , Andrew James Carson , Stanley S. Hong , Jason Bryant , Mark Wang , Drew Verkade
IPC分类号： C12M3/00 , B01L9/00 , B01L3/00
CPC分类号： B01L3/502715 , B01L7/52 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/04 , B01L2200/0689 , B01L2300/022 , B01L2300/041 , B01L2300/043 , B01L2300/0816 , B01L2300/0877 , G01N21/05 , G01N2021/058 , Y10T436/25
摘要： A fluidic device holder configured to orient a fluidic device. The device holder includes a support structure configured to receive a fluidic device. The support structure includes a base surface that faces in a direction along the Z-axis and is configured to have the fluidic device positioned thereon. The device holder also includes a plurality of reference surfaces facing in respective directions along an XY-plane. The device holder also includes an alignment assembly having an actuator and a movable locator arm that is operatively coupled to the actuator. The locator arm has an engagement end. The actuator moves the locator arm between retracted and biased positions to move the engagement end away from and toward the reference surfaces. The locator arm is configured to hold the fluidic device against the reference surfaces when the locator arm is in the biased position.
摘要翻译：流体装置保持器，其构造成定向流体装置。装置保持器包括构造成接收流体装置的支撑结构。所述支撑结构包括在Z轴方向上面对并且被配置成具有定位在其上的流体装置的基面。装置保持器还包括沿着XY平面在各个方向上面对的多个参考表面。装置保持器还包括具有致动器和可操作地联接到致动器的可动定位臂的对准组件。定位臂具有接合端。致动器将定位臂移动到缩回位置和偏置位置之间，以使接合端远离参考表面移动。定位臂构造成当定位臂处于偏置位置时将流体装置保持在参考表面上。

3. 发明授权

US08676383B2 Device for carrying out chemical or biological reactions 有权
标题翻译：用于进行化学或生物反应的装置
公开(公告)号：US08676383B2
公开(公告)日：2014-03-18
申请号：US11850345
申请日：2007-09-05
申请人： Lim Hi Tan , Jew Kwee Ngui , Hon Siu Shin , Ui Leng Soh , Yang Hooi Kee , Hock Lai Khoo , Mark T. Reed , Ching Ong Lau , Wolfgang Heimberg
发明人： Lim Hi Tan , Jew Kwee Ngui , Hon Siu Shin , Ui Leng Soh , Yang Hooi Kee , Hock Lai Khoo , Mark T. Reed , Ching Ong Lau , Wolfgang Heimberg
IPC分类号： G05B21/00
CPC分类号： B01L7/52 , B01L3/50855 , B01L7/54 , B01L2200/147 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , B01L2300/1855 , C12Q1/686 , G01N21/6452
摘要： The invention relates to a device for carrying out of chemical or biological reactions with a reaction vessel receiving element for receiving a microtiter plate with several reaction vessels, wherein the reaction vessel receiving element has several recesses arranged in a regular pattern to receive the respective reaction vessels, a heating device for heating the reaction vessel receiving element, and a cooling device for cooling the reaction vessel. The invention is characterized by the fact that the reaction vessel receiving element is divided into several segments. The individual segments are thermally decoupled from one another, and each segment is assigned a heating device which may be actuated independently of the others. By means of the segmentation of the reaction vessel receiving element, it is possible for zones to be set and held at different temperatures. Because the reaction vessel receiving element is suitable for receiving standard microtiter plates, the device according to the invention may be integrated in existing process sequences.
摘要翻译：本发明涉及一种用于与反应容器接收元件进行化学或生物反应的装置，用于接收具有几个反应容器的微量滴定板，其中反应容器接收元件具有以规则图案排列以容纳相应的反应容器，用于加热反应容器接收元件的加热装置和用于冷却反应容器的冷却装置。本发明的特征在于将反应容器接收元件分成几段。各个片段彼此热分离，并且每个片段被分配加热装置，其可以独立于其它片段致动。通过反应容器接收元件的分割，可以将区域设定并保持在不同的温度。因为反应容器接收元件适于接收标准微量滴定板，所以根据本发明的装置可以集成在现有的工艺顺序中。

4. 发明授权

US07670834B2 Gas thermal cycler 有权
标题翻译：气体热循环仪
公开(公告)号：US07670834B2
公开(公告)日：2010-03-02
申请号：US11166958
申请日：2005-06-23
申请人： Adrian Fawcett , Mark T. Reed
发明人： Adrian Fawcett , Mark T. Reed
IPC分类号： C12M1/00 , C12M3/00
CPC分类号： B01L3/50855 , B01L3/50851 , B01L7/52 , B01L2300/0654 , B01L2300/1827 , B01L2300/1838 , B01L2300/1844 , B01L2300/1894 , F28F1/16
摘要： The present application relates to an apparatus and method for thermal cycling using a source of cooling gas.
摘要翻译：本申请涉及一种使用冷却气体源的热循环的装置和方法。

5. 发明授权

US09052298B2 High density plate filler 有权
标题翻译：高密度板填料
公开(公告)号：US09052298B2
公开(公告)日：2015-06-09
申请号：US13049686
申请日：2011-03-16
申请人： Mark T. Reed , Albert L. Carrillo , Ian A. Harding
发明人： Mark T. Reed , Albert L. Carrillo , Ian A. Harding
IPC分类号： B01L3/00 , G01N35/02 , G01N21/64 , B01L3/02 , B01L7/00 , B01L9/00 , G01N21/27
CPC分类号： G01N35/028 , B01L3/0293 , B01L3/5025 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L7/52 , B01L9/50 , B01L9/523 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1805 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , G01N21/274 , G01N21/6452
摘要： A filling apparatus for filling a microplate. The microplate can comprise a plurality of wells each sized to receive an assay. A substrate can comprise a first surface and an opposing second surface, a first assay input port for receiving the assay disposed on the first surface, a plurality of staging capillaries extending through the substrate, and a first plurality of microfluidic channels fluidly coupling the first assay input port with at least one of the plurality of staging capillaries. Each of the plurality of staging capillaries can comprise an inlet and an outlet and be sized to receive the assay.
摘要翻译：一种用于填充微板的填充装置。微量培养板可以包括多个孔，每个孔的尺寸适于接受测定。基底可以包括第一表面和相对的第二表面，用于接收设置在第一表面上的测定的第一测定输入端口，延伸穿过基底的多个分期毛细血管，以及第一多个微流体通道，其将第一测定输入端口与多个分段毛细管中的至少一个。多个分级毛细管中的每一个可以包括入口和出口，并且其大小适于接收测定。

6. 发明授权

US07998435B2 High density plate filler 有权
标题翻译：高密度板填料
公开(公告)号：US07998435B2
公开(公告)日：2011-08-16
申请号：US11393061
申请日：2006-03-30
申请人： Mark T. Reed
发明人： Mark T. Reed
IPC分类号： B01L3/00
CPC分类号： G01N35/1074 , B01L3/0293 , B01L3/5025 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0684 , B01L2300/0829 , B01L2300/0864 , B01L2300/1805 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , Y10T436/2575
摘要： A filling apparatus for filling a microplate. The microplate having a plurality of wells each sized to receive an assay. The filling apparatus can comprise an output layer having a plurality of capillaries, wherein a first grouping of the capillaries is separated from a second grouping by a hydrophobic feature. Each of the plurality of capillaries can comprise an inlet and an outlet. A funnel assembly can comprise a funnel member sized to receive the assay. The funnel member can comprise an outlet for delivering a fluid bead of the assay along a top surface of the output layer and in fluid communication with each of the plurality of capillaries such that a portion of the fluid bead can be drawn within at least some of the plurality of capillaries in response to capillary force. The funnel assembly and the output layer can be moveable relative to each other between a first position and a second position to draw the fluid bead across the top surface.
摘要翻译：一种用于填充微板的填充装置。微量培养板具有多个孔，每个孔的大小用于接受测定。填充设备可以包括具有多个毛细管的输出层，其中毛细管的第一组通过疏水特征与第二组分离。多个毛细管中的每一个可以包括入口和出口。漏斗组件可以包括尺寸适于接收测定的漏斗构件。漏斗构件可以包括用于沿着输出层的顶表面递送测定的流体珠的出口，并且与多个毛细管中的每一个流体连通，使得流体珠的一部分可以在至少一些多个毛细管响应于毛细管力。漏斗组件和输出层可以在第一位置和第二位置之间相对于彼此移动，以便在顶表面上拉动流体珠。

7. 发明申请

US20110072914A1 Flow Cells And Manifolds Having An Electroosmotic Pump 有权
标题翻译：具有电渗泵的流动池和歧管
公开(公告)号：US20110072914A1
公开(公告)日：2011-03-31
申请号：US12031054
申请日：2008-02-14
申请人： Michal Lebl , Dale Buermann , Mark T. Reed , David L. Heiner , Alexander Triener
发明人： Michal Lebl , Dale Buermann , Mark T. Reed , David L. Heiner , Alexander Triener
IPC分类号： G01N1/14 , F04B37/10 , B01L3/02
CPC分类号： F04B19/006 , B01L3/502715 , B01L3/50273 , B01L7/00 , B01L2300/0816 , B01L2300/0877 , B01L2400/0418 , G01N35/00613 , G01N35/1095 , Y10T436/2575
摘要： A flows cell for use in a microfluidic detection system is provided. The flow cell includes a flows cell body having a channel that is configured to convey a solution through the flows cell body. The flow cell also includes a bottom surface and a top surface. The bottom surface is configured to be removably held by the detection system and the top surface is transparent and permits light to pass therethrough. The flow cell body also includes fluidic inlet and outlet ports that are in fluid communication with the channel. A pump cavity is also provided in the flow cell body. The pump cavity fluidly communicates with, and is interposed between, an end of the channel and one of the fluidic inlet and outlet ports. An electroosmotic (EO) pump is held in the pump cavity. The EO pump induces flow of the solution through the EO pump and channel between the fluidic inlet and outlet ports.
摘要翻译：提供了一种用于微流体检测系统的流通池。流动池包括具有通道的流动池主体，该通道被配置为将溶液输送通过流动池主体。流动池还包括底面和顶面。底表面被构造成由检测系统可移除地保持，并且顶表面是透明的并且允许光通过。流动池体还包括与通道流体连通的流体入口和出口。在流动池主体中还设有泵腔。泵腔与通道的端部和流体入口和出口之一流体连通并且介于其间。电渗（EO）泵保持在泵腔中。 EO泵通过EO泵和流体入口和出口之间的通道引起溶液的流动。

8. 发明授权

US07695688B2 High density plate filler 有权
标题翻译：高密度板填料
公开(公告)号：US07695688B2
公开(公告)日：2010-04-13
申请号：US11086273
申请日：2005-03-22
申请人： Mark T. Reed , Albert L. Carrillo , Ian A. Harding
发明人： Mark T. Reed , Albert L. Carrillo , Ian A. Harding
IPC分类号： B01L11/00
CPC分类号： G01N35/028 , B01L3/0293 , B01L3/5025 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L7/52 , B01L9/50 , B01L9/523 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1805 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , G01N21/274 , G01N21/6452
摘要： A filling apparatus for filling a microplate. The microplate can comprise a plurality of wells each sized to receive an assay. A substrate can comprise a first surface and an opposing second surface, a first assay input port for receiving the assay disposed on the first surface, a plurality of staging capillaries extending through the substrate, and a first plurality of microfluidic channels fluidly coupling the first assay input port with at least one of the plurality of staging capillaries. Each of the plurality of staging capillaries can comprise an inlet and an outlet and be sized to receive the assay.
摘要翻译：一种用于填充微板的填充装置。微量培养板可以包括多个孔，每个孔的尺寸适于接受测定。基底可以包括第一表面和相对的第二表面，用于接收设置在第一表面上的测定的第一测定输入端口，延伸穿过基底的多个分期毛细血管，以及第一多个微流体通道，其将第一测定输入端口与多个分段毛细管中的至少一个。多个分级毛细管中的每一个可以包括入口和出口，并且其大小适于接收测定。

9. 发明申请

US20090191098A1 High Density Plate Filler 审中-公开
标题翻译：高密度平板填料
公开(公告)号：US20090191098A1
公开(公告)日：2009-07-30
申请号：US12419015
申请日：2009-04-06
申请人： Nigel P. Beard , Mark T. Reed
发明人： Nigel P. Beard , Mark T. Reed
IPC分类号： B01L3/00
CPC分类号： G01N35/028 , B01L3/0268 , B01L3/0293 , B01L3/5025 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0621 , B01L2200/0642 , B01L2200/0684 , B01L2300/046 , B01L2300/0829 , B01L2300/0864 , B01L2300/1805 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , G01N35/1074 , G01N2035/1034
摘要： A filling apparatus for filling a microplate. The microplate having a plurality of wells each sized to receive an assay. The filling apparatus can comprise an assay input layer having a first surface and an opposing second surface. The assay input layer can comprise an assay input port extending from the first surface to the second surface and at least one pressure nodule extending from the second surface. An output layer can comprise a plurality of staging capillaries each having an inlet and an outlet. The output layer can further comprise a capillary plane disposed above the plurality of staging capillaries in fluid communication with the assay input port. The capillary plane can be sized to draw the assay from the assay input port to generally flood fill the plurality of staging capillaries.
摘要翻译：一种用于填充微板的填充装置。微量培养板具有多个孔，每个孔的大小用于接受测定。填充装置可以包括具有第一表面和相对的第二表面的测定输入层。测定输入层可以包括从第一表面延伸到第二表面的测定输入端口和从第二表面延伸的至少一个压力结节。输出层可以包括多个分级毛细管，每个具有入口和出口。输出层还可以包括设置在多个分级毛细管上方的毛细管平面，其与分析输入端口流体连通。毛细管平面的尺寸可以从测定输入端口抽取测定值，以通常灌注多个分期毛细血管。

10. 发明授权

US06451261B1 Multi-well microfiltration apparatus 失效
公开(公告)号：US06451261B1
公开(公告)日：2002-09-17
申请号：US09565673
申请日：2000-05-04
申请人： Kevin S. Bodner , Alfred P. Madden , Joseph Jackson , Jason H. Halsey , Mark T. Reed , Ward Frye , Mark F. Oldham , Stephen E. Moring , Jon Hoshizaki
发明人： Kevin S. Bodner , Alfred P. Madden , Joseph Jackson , Jason H. Halsey , Mark T. Reed , Ward Frye , Mark F. Oldham , Stephen E. Moring , Jon Hoshizaki
IPC分类号： B01L300
CPC分类号： B01L3/5025 , B01D61/18 , B01L3/50255 , B01L2400/049 , G01N35/0099 , G01N35/028 , G01N35/1074 , G01N2035/00485 , G01N2035/102 , Y10T436/25
摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.

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