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1. 发明授权

US08597594B2 Apparatus for fragmenting nucleic acids 有权
标题翻译：破碎核酸的装置
公开(公告)号：US08597594B2
公开(公告)日：2013-12-03
申请号：US13551704
申请日：2012-07-18
申请人： Jonathan Posner , Kamil Salloum , Michal Lebl , Mark Reed , Dale Buermann , Matthew Hage , Bryan Crane , David Heiner , Robert Kain , Michael Schroeder
发明人： Jonathan Posner , Kamil Salloum , Michal Lebl , Mark Reed , Dale Buermann , Matthew Hage , Bryan Crane , David Heiner , Robert Kain , Michael Schroeder
IPC分类号： B01L3/00
CPC分类号： F04B37/10 , F04B19/00 , Y10T436/2575
摘要： An apparatus for fragmenting nucleic acid. The apparatus includes a sample reservoir that comprises a fluid having nucleic acids. The apparatus can also include a shear wall that is positioned within the sample reservoir. The shear wall includes a porous core medium that has pores that are sized to permit nucleic acids to flow therethrough. The apparatus also includes first and second chambers that are separated by the shear wall. The first and second chambers are in fluid communication with each other through the porous core medium of the shear wall. Also, the apparatus may include first and second electrodes that are located within the first and second chambers, respectively. The first and second electrodes are configured to generate an electric field that induces a flow of the sample fluid. The nucleic acids move through the shear wall thereby fragmenting the nucleic acids.
摘要翻译：核酸分裂装置。该装置包括包含具有核酸的流体的样品储存器。该装置还可以包括位于样品容器内的剪切壁。剪切壁包括多孔芯介质，其具有尺寸允许核酸流过其中的孔。该装置还包括被剪力墙隔开的第一和第二室。第一和第二腔室通过剪切壁的多孔纤维介质彼此流体连通。此外，该装置可以包括分别位于第一和第二室内的第一和第二电极。第一和第二电极被配置成产生诱发样品流体流动的电场。核酸通过剪切壁移动，从而使核酸分裂。

2. 发明申请

US20120223025A1 APPARATUS AND METHOD FOR SEPARATION OF LIQUID PHASES OF DIFFERENT DENSITY AND FOR FLUOROUS PHASE ORGANIC SYNTHESES 有权
标题翻译：用于分离不同密度的液相和氟相有机合成的装置和方法
公开(公告)号：US20120223025A1
公开(公告)日：2012-09-06
申请号：US13470130
申请日：2012-05-11
申请人： Michal Lebl
发明人： Michal Lebl
IPC分类号： B01D24/32 , B01D24/36
CPC分类号： C07K1/045 , B01D17/0202 , B01D17/0217 , B01J19/0046 , B01J2219/00315 , B01J2219/00326 , B01J2219/00351 , B01J2219/00421 , B01J2219/00466 , B01J2219/0049 , B01J2219/005 , B01J2219/00511 , B01J2219/00585 , B01J2219/00596 , B01J2219/00599 , B01J2219/00605 , B01J2219/00621 , B01J2219/00626 , B01J2219/00659 , B01J2219/00689 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B04B5/0407 , B04B5/0421 , C07K1/04 , C40B40/06 , C40B40/10 , C40B50/08 , C40B60/14 , G01N35/028 , G01N2035/00495
摘要： A simple, efficient apparatus and method for separating layers of immiscible or partially miscible liquids useful in methods of high-throughput combinatorial organic synthesis or parallel extraction of large libraries or megaarrays of organic compounds is disclosed. The apparatus and method are useful, whether as part of an automated, robotic or manual system for combinatorial organic synthesis or purification (extraction). In a preferred embodiment, an apparatus and method for separating layers of immiscible or partially miscible liquids compatible with microtiter plate type array(s) of reaction vessels is disclosed. Another application of centrifugation based liquid removal was found for washing the plates in biological assays or synthesis on modified substrates.
摘要翻译：公开了一种简单有效的装置和方法，用于分离用于高通量组合有机合成或平行提取大型文库或有机化合物的大量阵列的方法中的不混溶或部分混溶液体层。该装置和方法无论作为组合有机合成或纯化（提取）的自动机器人或手动系统的一部分是有用的。在优选实施例中，公开了用于分离与微量滴定板型阵列的反应容器相容的不混溶或部分可混溶液体的层的装置和方法。发现基于离心的液体去除的另一种应用是在生物测定中进行洗涤或在改性底物上进行合成。

3. 发明申请

US20110143965A1 OLIGONUCLEOTIDE SYNTHESIZER 有权
标题翻译：寡核苷酸合成酶
公开(公告)号：US20110143965A1
公开(公告)日：2011-06-16
申请号：US13023406
申请日：2011-02-08
申请人： Michal Lebl , Mark S. Chee , Steven M. Barnard , David L. Heiner , Vit Pokorny , Mark J. Nibbe , Aaron Christopher Jones
发明人： Michal Lebl , Mark S. Chee , Steven M. Barnard , David L. Heiner , Vit Pokorny , Mark J. Nibbe , Aaron Christopher Jones
IPC分类号： C40B50/08 , C40B60/14
CPC分类号： B01J19/0046 , B01J2219/00283 , B01J2219/00286 , B01J2219/00295 , B01J2219/00308 , B01J2219/00315 , B01J2219/00326 , B01J2219/00328 , B01J2219/00369 , B01J2219/00389 , B01J2219/00412 , B01J2219/00421 , B01J2219/00423 , B01J2219/00454 , B01J2219/0049 , B01J2219/005 , B01J2219/00585 , B01J2219/0059 , B01J2219/00689 , B01J2219/0072 , B01J2219/00722 , C40B40/06 , C40B50/14 , C40B60/14 , G01N35/025 , G01N35/028 , G01N35/1067 , G01N2035/00504 , G01N2035/1037 , G01N2035/1069 , Y10T436/111666 , Y10T436/12 , Y10T436/2575
摘要： An apparatus for high-throughput combinatorial synthesis of organic molecules including a reaction vessel for containing a combinatorial chemistry synthetic reaction, a liquid dispenser for dispensing the liquid, a liquid aspirator and an adjustment mechanism. The reaction vessel includes an ingress aperture allowing a liquid to enter into an interior of the vessel and an egress aperture for aspirating the liquid from the vessel. The liquid dispenser dispenses liquid through the ingress aperture. The liquid aspirator aspirates liquid through the egress aperture and includes a rotor for carrying the vessel and orbiting the vessel about an axis of rotation. The rotor is oriented generally in a horizontal plane and includes an adjustment mechanism for adjusting the angle of the vessel relative to the horizontal plane in response to the centrifugal force generated by orbiting the vessel about the axis or rotation. A method of combinatorial synthesis of organic molecules is also disclosed.
摘要翻译：一种用于高通量组合合成有机分子的装置，包括用于包含组合化学合成反应的反应容器，用于分配液体的液体分配器，液体抽吸器和调节机构。反应容器包括允许液体进入容器内部的入口孔和用于从容器吸出液体的出口孔。液体分配器通过入口孔分配液体。液体吸入器通过出口孔吸出液体，并且包括用于承载容器并绕容器围绕旋转轴线运动的转子。转子通常在水平面定向，并且包括调节机构，用于响应于通过围绕轴线或旋转绕过容器而产生的离心力来调节容器相对于水平面的角度。还公开了组合合成有机分子的方法。

4. 发明授权

US06663832B2 Oligonucleotide synthesizer 有权
公开(公告)号：US06663832B2
公开(公告)日：2003-12-16
申请号：US09738473
申请日：2000-12-13
申请人： Michal Lebl , Mark S. Chee , Steven M. Barnard , David L. Heiner , Vit Pokorny , Mark J. Nibbe , Aaron Christopher Jones
发明人： Michal Lebl , Mark S. Chee , Steven M. Barnard , David L. Heiner , Vit Pokorny , Mark J. Nibbe , Aaron Christopher Jones
IPC分类号： G01N2100
CPC分类号： C40B50/14 , B01J19/0046 , B01J2219/00283 , B01J2219/00286 , B01J2219/00295 , B01J2219/00308 , B01J2219/00315 , B01J2219/00326 , B01J2219/00328 , B01J2219/00369 , B01J2219/00389 , B01J2219/00412 , B01J2219/00421 , B01J2219/00423 , B01J2219/00454 , B01J2219/0049 , B01J2219/005 , B01J2219/00585 , B01J2219/0059 , B01J2219/00689 , B01J2219/0072 , B01J2219/00722 , C40B40/06 , C40B60/14 , G01N35/025 , G01N35/028 , G01N35/1067 , G01N2035/00504 , G01N2035/1037 , G01N2035/1069 , Y10T436/11 , Y10T436/111666 , Y10T436/113332 , Y10T436/2575
摘要： An apparatus for high-throughput combinatorial syntheses of organic molecules including a reaction vessel for containing a combinatorial-chemistry synthetic reaction, a liquid dispenser for dispensing the liquid, a liquid aspirator and an adjustment mechanism. The reaction vessel includes an ingress aperture allowing a liquid to enter into an interior of the vessel and an egress aperture for aspirating the liquid from the vessel. The liquid dispenser dispenses liquid through the ingress aperture. The liquid aspirator aspirates liquid through the egress aperture and includes a rotor for carrying the vessel and orbiting the vessel about an axis of rotation. The rotor is oriented generally in a horizontal plane and includes an adjustment mechanism for adjusting the angle of the vessel relative to the horizontal plane in response to the centrifugal force generated by orbiting the vessel about the axis of rotation. A method of combinatorial synthesis of organic molecules is also disclosed.

5. 发明授权

US6121054A Method for separation of liquid and solid phases for solid phase organic syntheses 失效
标题翻译：固相有机合成液相和固相分离方法
公开(公告)号：US6121054A
公开(公告)日：2000-09-19
申请号：US974090
申请日：1997-11-19
申请人： Michal Lebl
发明人： Michal Lebl
IPC分类号： B04B1/00 , B01J19/00 , B04B5/04 , C07K1/04 , C40B60/14 , G01N35/00 , G01N35/02 , G01N1/18
CPC分类号： C07K1/045 , B01J19/0046 , B04B5/0407 , B01J2219/00315 , B01J2219/00421 , B01J2219/00461 , B01J2219/0049 , B01J2219/005 , B01J2219/00585 , B01J2219/00596 , B01J2219/0072 , C40B60/14 , G01N2035/00504 , G01N35/028 , Y10T436/111666 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
摘要： A simple, efficient apparatus and method for separation of solid and liquid phases useful in methods of high-throughput combinatorial organic synthesis of large libraries or megaarrays of organic compounds is disclosed. The apparatus and method are useful, whether as part of an automated, robotic or manual system for combinatorial organic synthesis. In a preferred embodiment, an apparatus and method of removal of liquid phase from solid phase compatible with microtiter plate type array(s) of reaction vessels is disclosed.
摘要翻译：公开了一种用于分离固相和液相的简单，有效的装置和方法，其用于大量文库或大型有机化合物的高通量组合有机合成方法。该装置和方法无论作为组合有机合成的自动机器人或手动系统的一部分是有用的。在一个优选的实施方案中，公开了一种从微量滴定板型的反应容器阵列相容的固相中除去液相的装置和方法。

6. 发明授权

US6090912A Topologically segregated, encoded solid phase libraries comprising linkers having an enzymatically susceptible bond 有权
标题翻译：包含具有酶敏感键的接头的拓扑分离的编码固相文库
公开(公告)号：US6090912A
公开(公告)日：2000-07-18
申请号：US198209
申请日：1998-11-23
申请人： Michal Lebl , Kit S. Lam , Sydney E. Salmon , Victor Krchnak , Nikolai Sepetov , Peter Kocis
发明人： Michal Lebl , Kit S. Lam , Sydney E. Salmon , Victor Krchnak , Nikolai Sepetov , Peter Kocis
IPC分类号： G01N33/53 , C07B61/00 , C07K1/04 , C07K17/00 , C40B70/00 , G01N33/543 , G01N33/566 , C07K4/00 , C07K1/00 , C12N9/48
CPC分类号： C07K1/047 , B01J2219/00572 , B01J2219/00596 , C40B70/00
摘要： The invention relates to libraries of synthetic test compound attached to separate phase synthesis supports. In particular, the invention relates to libraries of synthetic test compound attached to separate phase synthesis supports that also contain coding molecules that encode the structure of the synthetic test compound. The molecules may be polymers or multiple nonpolymeric molecules. Each of the solid phase synthesis support beads contains a single type of synthetic test compound. The synthetic test compound can have backbone structures with linkages such as amide, urea, carbamate (i.e., urethane), ester, amino, sulfide, disulfide, or carbon--carbon, such as alkane and alkene, or any combination thereof. Examples of subunits suited for the different linkage chemistries are provided. The synthetic test compound can also be molecular scaffolds, such as derivatives of monocyclic of bicyclic carbohydrates, steroids, sugars, heterocyclic structures, polyaromatic structures, or other structures capable of acting as a scaffolding. Examples of suitable molecular scaffolds are provided. The invention also relates to methods of synthesizing such libraries and the use of such libraries to identify and characterize molecules of interest from among the library of synthetic test compound.
摘要翻译：本发明涉及附着于分离相合成载体的合成试验化合物的文库。特别地，本发明涉及连接到分离相合成载体上的合成测试化合物文库，其中还包含编码合成测试化合物结构的编码分子。分子可以是聚合物或多个非聚合物分子。每个固相合成载体珠含有单一类型的合成试验化合物。合成试验化合物可以具有带有键合的主链结构，例如酰胺，脲，氨基甲酸酯（即氨基甲酸酯），酯，氨基，硫化物，二硫化物或碳 - 碳如烷烃和烯烃，或其任何组合。提供了适用于不同连接化学物质的亚基的实例。合成试验化合物还可以是分子支架，例如双环碳水化合物，类固醇，糖，杂环结构，多芳族结构或能够作为脚手架的其它结构的单环衍生物。提供了合适的分子支架的实例。本发明还涉及合成这些文库的方法，以及使用此类文库从合成测试化合物文库中鉴定和表征目标分子。

7. 发明授权

US5840485A Topologically segregated, encoded solid phase libraries 失效
标题翻译：拓扑分离，编码固相库
公开(公告)号：US5840485A
公开(公告)日：1998-11-24
申请号：US249830
申请日：1994-05-26
申请人： Michal Lebl , Kit S. Lam , Sydney E. Salmon , Victor Krchnak , Nikolai Sepetov , Peter Kocis
发明人： Michal Lebl , Kit S. Lam , Sydney E. Salmon , Victor Krchnak , Nikolai Sepetov , Peter Kocis
IPC分类号： C07K17/00 , C07K1/04 , C12P21/00 , C12Q1/00 , C40B70/00 , C12Q1/68 , C07H21/04 , C07K17/02 , G01N33/53
CPC分类号： C07K1/047 , B01J2219/00572 , B01J2219/00596 , C40B70/00
摘要： The invention relates to libraries of synthetic test compound attached to separate phase synthesis supports that also contain coding molecules that encode the structure of the synthetic test compound. The molecules may be polymers or multiple nonpolymeric molecules. The synthetic test compound can have backbone structures with linkages such as amide, urea, carbamate (i.e., urethane), ester, amino, sulfide, disulfide, or carbon-carbon, such as alkane and alkene, or any combination thereof. Examples of subunits suited for the different linkage chemistries are provided. The synthetic test compound can also be molecular scaffolds, such as derivatives of monocyclic of bicyclic carbohydrates, steroids, sugars, heterocyclic structures, polyaromatic structures, or other structures capable of acting as a scaffolding. Examples of suitable molecular scaffolds are provided. The invention also relates to methods of synthesizing such libraries and the use of such libraries to identify and characterize molecules of interest from among the library of synthetic test compound.
摘要翻译：本发明涉及连接到分离相合成载体上的合成试验化合物文库，其中还包含编码合成测试化合物结构的编码分子。分子可以是聚合物或多个非聚合物分子。合成试验化合物可以具有带有键合的主链结构，例如酰胺，脲，氨基甲酸酯（即氨基甲酸酯），酯，氨基，硫化物，二硫化物或碳 - 碳如烷烃和烯烃，或其任何组合。提供了适用于不同连接化学物质的亚基的实例。合成试验化合物还可以是分子支架，例如双环碳水化合物，类固醇，糖，杂环结构，多芳族结构或能够用作脚手架的其它结构的单环衍生物。提供了合适的分子支架的实例。本发明还涉及合成这些文库的方法，以及使用此类文库从合成测试化合物文库中鉴定和表征目标分子。

8. 发明授权

US08709717B2 Generation of uniform fragments of nucleic acids using patterned substrates 有权
标题翻译：使用图案化底物生成核酸的均匀片段
公开(公告)号：US08709717B2
公开(公告)日：2014-04-29
申请号：US13260205
申请日：2010-04-02
申请人： Kevin Gunderson , Michal Lebl , David L. Heiner
发明人： Kevin Gunderson , Michal Lebl , David L. Heiner
IPC分类号： C12Q1/68 , C12N11/02 , C40B50/04 , C40B60/06 , B01D57/02
CPC分类号： C12N15/10 , C12N15/101 , Y10T436/143333
摘要： Methods of generating nucleic acid fragments of substantially uniform length from sample nucleic acids comprising linearly stretching the sample nucleic acids over a substrate having a plurality of cleavage regions separated by relatively consistent distances, cleaving the linearly stretched sample nucleic acids at the cleavage regions, and collecting the resulting nucleic acid fragments. The method may further include collecting and concentrating the resultant nucleic acid fragments of substantially uniform length.
摘要翻译：从样品核酸产生具有基本上均匀长度的核酸片段的方法，包括在具有通过相对一致距离分离的多个切割区域的底物上线性拉伸样品核酸，在切割区域切割线性拉伸的样品核酸，并收集得到的核酸片段。该方法还可以包括收集和浓缩所得到的基本上均匀长度的核酸片段。

9. 发明授权

US08178652B2 Apparatus and method for separation of liquid phases of different density and for fluorous phase organic syntheses 有权
标题翻译：用于分离不同密度的液相和氟相有机合成的装置和方法
公开(公告)号：US08178652B2
公开(公告)日：2012-05-15
申请号：US13168131
申请日：2011-06-24
申请人： Michal Lebl
发明人： Michal Lebl
IPC分类号： A61K33/00
CPC分类号： C07K1/045 , B01D17/0202 , B01D17/0217 , B01J19/0046 , B01J2219/00315 , B01J2219/00326 , B01J2219/00351 , B01J2219/00421 , B01J2219/00466 , B01J2219/0049 , B01J2219/005 , B01J2219/00511 , B01J2219/00585 , B01J2219/00596 , B01J2219/00599 , B01J2219/00605 , B01J2219/00621 , B01J2219/00626 , B01J2219/00659 , B01J2219/00689 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B04B5/0407 , B04B5/0421 , C07K1/04 , C40B40/06 , C40B40/10 , C40B50/08 , C40B60/14 , G01N35/028 , G01N2035/00495
摘要： A simple, efficient apparatus and method for separating layers of immiscible or partially miscible liquids useful in methods of high-throughput combinatorial organic synthesis or parallel extraction of large libraries or megaarrays of organic compounds is disclosed. The apparatus and method are useful, whether as part of an automated, robotic or manual system for combinatorial organic synthesis or purification (extraction). In a preferred embodiment, an apparatus and method for separating layers of immiscible or partially miscible liquids compatible with microtiter plate type array(s) of reaction vessels is disclosed. Another application of centrifugation based liquid removal was found for washing the plates in biological assays or synthesis on modified substrates.
摘要翻译：公开了一种简单有效的装置和方法，用于分离用于高通量组合有机合成或平行提取大型文库或有机化合物的大量阵列的方法中的不混溶或部分混溶液体层。该装置和方法无论作为组合有机合成或纯化（提取）的自动机器人或手动系统的一部分是有用的。在优选实施例中，公开了用于分离与微量滴定板型阵列的反应容器相容的不混溶或部分可混溶液体的层的装置和方法。发现基于离心的液体去除的另一种应用是在生物测定中进行洗涤或在改性底物上进行合成。

10. 发明申请

US20100187115A1 ELECTROOSMOTIC PUMP WITH IMPROVED GAS MANAGEMENT 有权
标题翻译：具有改进气体管理的电动泵
公开(公告)号：US20100187115A1
公开(公告)日：2010-07-29
申请号：US12626353
申请日：2009-11-25
申请人： Jonathan Posner , Kamil Salloum , Michal Lebl , Mark Reed , Dale Buermann , Matthew Hage , Bryan Crane , David Heiner , Robert Kain
发明人： Jonathan Posner , Kamil Salloum , Michal Lebl , Mark Reed , Dale Buermann , Matthew Hage , Bryan Crane , David Heiner , Robert Kain
IPC分类号： B01D57/02 , F04B19/00 , B01D61/46 , B81B7/02 , C12Q1/68 , B01J19/00 , G01N27/447
CPC分类号： F04B37/10 , F04B19/00 , Y10T436/2575
摘要： An electroosmotic (EO) pump is provided that includes a housing having a pump cavity, a porous core medium and electrodes. The porous core medium is positioned within the pump cavity to form an exterior reservoir that extends at least partially about an exterior surface of the porous core medium. The porous core medium has an open inner chamber provided therein. The inner chamber represents an interior reservoir. The electrodes are positioned in the inner chamber and are positioned proximate the exterior surface. The electrodes induce flow of a fluid through the porous core medium between the interior and exterior reservoirs, wherein a gas is generated when the electrodes induce flow of the fluid. The housing has a fluid inlet to convey the fluid to one of the interior reservoir and the exterior reservoir. The housing has a fluid outlet to discharge the fluid from another of the interior reservoir and the exterior reservoir. The housing has a gas removal device to remove the gas from the pump cavity.
摘要翻译：提供一种电渗（EO）泵，其包括具有泵腔的壳体，多孔芯介质和电极。多孔芯介质定位在泵腔内以形成至少部分地围绕多孔芯介质的外表面延伸的外部储存器。多孔芯介质具有设置在其中的开放内室。内室表示内部储存器。电极定位在内室中并且位于外表面附近。电极通过多孔芯介质在内部和外部储存器之间引导流体流动，其中当电极引起流体流动时产生气体。壳体具有用于将流体输送到内部储存器和外部储存器中的一个的流体入口。壳体具有流体出口，以从另一个内部储存器和外部储存器排出流体。壳体具有用于从泵腔去除气体的气体去除装置。

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