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1. 发明申请

WO2013040353A1 PLANTS HAVING STRESS RELATED TRAITS AND METHODS FOR MAKING THE SAME 审中-公开
标题翻译：具有应力相关行为的植物及其制备方法
公开(公告)号：WO2013040353A1
公开(公告)日：2013-03-21
申请号：PCT/US2012/055409
申请日：2012-09-14
申请人： DOW AGROSCIENCES LLC , REDDY, Avutu S. , YUEJIN, Sun , MERLO, Donald J. , NI, Weiting W. , PAREDDY, Dayakar , GAO, Zhifang , BRADFISCH, Gregory A.
发明人： REDDY, Avutu S. , YUEJIN, Sun , MERLO, Donald J. , NI, Weiting W. , PAREDDY, Dayakar , GAO, Zhifang , BRADFISCH, Gregory A.
IPC分类号： C12N15/82 , C12N15/87
CPC分类号： C07K14/415 , C12N9/93 , C12N15/8273 , C12Y603/02019
摘要： This invention relates generally to a plant cell with increased yield, preferably under condition of transient and repetitive abiotic stress as compared to a corresponding non-transformed wild type plant cell by increasing or generating one or more activities of ring proteins in plants.
摘要翻译：本发明一般涉及通过增加或产生植物中环蛋白的一种或多种活性而与相应的未转化的野生型植物细胞相比，优选在瞬时和重复的非生物胁迫的条件下具有增加的产量的植物细胞。

2. 发明申请

WO2012016222A3 STRAINS OF AGROBACTERIUM MODIFIED TO INCREASE PLANT TRANSFORMATION FREQUENCY 审中-公开
标题翻译：农杆菌菌株改良以提高植物转化频率
公开(公告)号：WO2012016222A3
公开(公告)日：2012-06-21
申请号：PCT/US2011046028
申请日：2011-07-29
申请人： DOW AGROSCIENCES LLC , MERLO DONALD J , RUSSELL SEAN M , RETALLACK DIANE , WOOSLEY AARON , MEADE TOM , NARVA KENNETH E
发明人： MERLO DONALD J , RUSSELL SEAN M , RETALLACK DIANE , WOOSLEY AARON , MEADE TOM , NARVA KENNETH E
IPC分类号： A01H5/00 , C12N1/21 , C12N15/82 , C12N15/84
CPC分类号： C12N15/8205 , C07K14/195 , C07K14/325 , C12N15/74 , C12N15/743 , C12N15/8274 , C12N15/8286 , Y02A40/162
摘要： Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.
摘要翻译：提供了在能够独立于农杆菌染色体复制的质粒上携带转化增强基因的农杆菌菌株，Ti质粒和植物转化双元载体，以及这些农杆菌菌株的用途。此外，导致植物转化双元载体不稳定或重排的DNA重组功能缺陷的农杆菌菌株，以及在能够独立于农杆菌染色体，Ti质粒和植物转化二元体复制的质粒上携带转化增强基因载体，以及这些菌株的用途。进一步包括的是农杆菌菌株，其具有整合到农杆菌染色体中的转化增强基因，其不会干扰土壤杆菌细胞的正常生长和植物转化能力或者以其它方式危害土壤杆菌细菌的植物转化能力，以及这些农杆菌菌株的用途。还描述了使用这些农杆菌菌株制造的植物。

3. 发明申请

WO2008070845A3 NOVEL SELECTABLE MARKER GENES 审中-公开
公开(公告)号：WO2008070845A3
公开(公告)日：2008-06-12
申请号：PCT/US2007/086813
申请日：2007-12-07
申请人： DOW AGROSCIENCES LLC , LIRA, Justin, M. , WRIGHT, Terry, R. , RUSSELL, Sean, M. , MERLO, Donald, J. , WEBB, Steven, Robert , ARNOLD, Nicole, L. , ROBINSON, Andrew, E. , SMITH, Kelley, A.
发明人： LIRA, Justin, M. , WRIGHT, Terry, R. , RUSSELL, Sean, M. , MERLO, Donald, J. , WEBB, Steven, Robert , ARNOLD, Nicole, L. , ROBINSON, Andrew, E. , SMITH, Kelley, A.
IPC分类号： C12N1/21 , C12N15/82 , A01H1/00
摘要： The subject invention relates to a novel gene referred to herein as DSM-2 . This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.

4. 发明申请

WO2007053482A2 NOVEL HERBICIDE RESISTANCE GENES 审中-公开
标题翻译：新型抗除草剂基因
公开(公告)号：WO2007053482A2
公开(公告)日：2007-05-10
申请号：PCT/US2006/042133
申请日：2006-10-27
申请人： DOW AGROSCIENCES LLC , WRIGHT, Terry, R. , LIRA, Justin, M. , WALSH, Terence, Anthony , MERLO, Donald, J. , JAYAKUMAR, Pon, Samuel , LIN, Gaofeng
发明人： WRIGHT, Terry, R. , LIRA, Justin, M. , WALSH, Terence, Anthony , MERLO, Donald, J. , JAYAKUMAR, Pon, Samuel , LIN, Gaofeng
IPC分类号： A01H5/00
CPC分类号： C12N15/8274 , A01N39/04 , C12N9/0069 , C12N9/0071 , C12N15/8275 , C12Q1/6895 , C12Q2600/13 , C12Q2600/158 , C12Y113/11
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention "stacked" together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 ( A ryloxy A lkanoate D ioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.
摘要翻译：本发明提供了不仅对2,4-D有抗性的新型植物，而且还涉及吡啶氧基乙酸酯除草剂。迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。本发明还包括与一种或多种其它除草剂抗性基因一起产生本发明一种或多种酶“堆叠”的植物。本发明能够以新的方式使用除草剂的新型组合。此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性的杂草的发育和控制菌株的新方法。根据本发明使用的优选酶和基因在本文中称为AAD-12（丙烯酰氧基烷酸酯加氧酶）。这种高度新颖的发现是显着的除草剂耐受性作物性状和选择性标记机会的基础。

5. 发明申请

WO2006094161A3 NEW SOURCES FOR, AND TYPES OF, INSECTICIDALLY ACTIVE PROTEINS, AND POLYNUCLEOTIDES THAT ENCODE THE PROTEINS 审中-公开
标题翻译：新的来源和类型的杀虫活性蛋白质和编码蛋白质的多核苷酸
公开(公告)号：WO2006094161A3
公开(公告)日：2007-03-08
申请号：PCT/US2006007524
申请日：2006-03-02
申请人： DOW AGROSCIENCES LLC , LARRINUA IGNACIO M , MERLO DONALD J , WOOSLEY AARON TODD , HEY TIMOTHY D , MEADE THOMAS , BURTON STEPHANIE LOVE
发明人： LARRINUA IGNACIO M , MERLO DONALD J , WOOSLEY AARON TODD , HEY TIMOTHY D , MEADE THOMAS , BURTON STEPHANIE LOVE
IPC分类号： A01N65/00 , C12N15/82 , C07K14/195 , C07K14/37
CPC分类号： C07K14/195 , A01N63/00 , A01N63/04 , C07K14/20 , C07K14/21 , C07K14/37 , C07K16/14 , C07K2319/00 , Y10S530/858
摘要： The subject invention provides exciting new sources for surprising, new types of toxin complex ("TC") proteins. The subject invention includes these new classes and types of TC proteins. The subject invention also includes polynucleotides that encode the subject proteins. The subject invention further provides vectors and cells comprising these polynucleotides. The subject invention also provides novel methods of controlling insects. The subject invention relates in part to the surprising discovery that new types of TC proteins can be obtained from a widely diverse phylogenetic spectrum of organisms including, most notably and surprisingly, eukaryotic fungus.
摘要翻译：本发明为令人惊奇的新型毒素复合物（“TC”）蛋白质提供令人兴奋的新来源。本发明包括这些新类型和TC蛋白。本发明还包括编码受试蛋白的多核苷酸。本发明进一步提供了包含这些多核苷酸的载体和细胞。本发明还提供了控制昆虫的新方法。本发明部分地涉及令人惊奇的发现，即可以从广泛多样的生物学系统发育谱获得新型TC蛋白质，包括最引人注目和令人惊讶的真核真菌。

6. 发明申请

WO2005107437A3 NOVEL HERBICIDE RESISTANCE GENES 审中-公开
标题翻译：新型抗除草剂基因
公开(公告)号：WO2005107437A3
公开(公告)日：2006-06-15
申请号：PCT/US2005014737
申请日：2005-05-02
申请人： DOW AGROSCIENCES LLC , WRIGHT TERRY R , LIRA JUSTIN M , MERLO DONALD J , HOPKINS NICOLE
发明人： WRIGHT TERRY R , LIRA JUSTIN M , MERLO DONALD J , HOPKINS NICOLE
IPC分类号： A01H5/00 , C12N9/02 , C12N15/82
CPC分类号： C12N15/8274 , A01N37/38 , A01N43/60 , A01N57/20 , C12N9/0069 , C12N9/0071 , C12N15/8209 , C12N15/8241 , C12N15/8247 , C12N15/8249 , C12N15/8255 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8275 , C12N15/8282 , C12N15/8283 , C12N15/8286
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or "stacked" together with another herbicide resistance gene, preferably a glyphosate. resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No a-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.
摘要翻译：本发明提供了不仅对2,4-D和其它苯氧基生长素除草剂具有抗性的新型植物，而且还涉及芳氧基苯氧基丙酸酯除草剂。迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。本发明还包括单独产生一种或多种本发明的酶或与另一除草剂抗性基因，优选草甘膦一起“堆叠”的植物。抗性基因，以提供更广泛和更强大的杂草控制，增加的治疗灵活性和改进的除草剂抗性管理选择。更具体地，根据本发明使用的优选的酶和基因在本文中称为AAD（芳氧基链烷酸酯加氧酶）基因和蛋白质。据报道，没有α-酮戊二酸依赖性双加氧酶具有降解不同化学类别和作用模式的除草剂的能力。这种高度新颖的发现是显着的除草剂耐受性作物性状机会以及可选择标记技术的发展的基础。本发明还包括控制杂草的相关方法。本发明能够以新的方式使用除草剂的新型组合。此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性（或天然更耐受）的杂草的形成和控制的新方法。

7. 发明申请

WO2005107437A2 NOVEL HERBICIDE RESISTANCE GENES 审中-公开
标题翻译：新型抗除草剂基因
公开(公告)号：WO2005107437A2
公开(公告)日：2005-11-17
申请号：PCT/US2005/014737
申请日：2005-05-02
申请人： DOW AGROSCIENCES LLC , WRIGHT, Terry, R. , LIRA, Justin, M. , MERLO, Donald, J. , HOPKINS, Nicole
发明人： WRIGHT, Terry, R. , LIRA, Justin, M. , MERLO, Donald, J. , HOPKINS, Nicole
IPC分类号： A01H5/00
CPC分类号： C12N15/8274 , A01N37/38 , A01N43/60 , A01N57/20 , C12N9/0069 , C12N9/0071 , C12N15/8209 , C12N15/8241 , C12N15/8247 , C12N15/8249 , C12N15/8255 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8275 , C12N15/8282 , C12N15/8283 , C12N15/8286
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or "stacked" together with another herbicide resistance gene, preferably a glyphosate. resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.
摘要翻译：本发明提供了不仅对2,4-D和其它苯氧基生长素除草剂具有抗性的新型植物，而且还涉及芳氧基苯氧基丙酸酯除草剂。迄今为止，没有任何期望或建议，通过引入单个基因可以产生具有这两个这些有利性质的植物。本发明还包括单独产生一种或多种本发明的酶或与其它除草剂抗性基因，优选草甘膦一起“堆叠”的植物。抗性基因，以提供更广泛和更强大的杂草控制，增加的治疗灵活性和改进的除草剂抗性管理选择。更具体地，根据本发明使用的优选的酶和基因在本文中被称为AAD（芳氧基链烷酸酯加氧酶）基因和蛋白质。据报道，没有α-酮戊二酸依赖性双加氧酶具有降解不同化学类别和作用模式的除草剂的能力。这种高度新颖的发现是显着的除草剂耐受性作物性状机会的基础以及可选择标记技术的发展。本发明还包括控制杂草的相关方法。本发明能够以新的方式使用除草剂的新型组合。此外，本发明提供了防止形成和控制对一种或多种除草剂（例如草甘膦）具有抗性（或天然更耐受）的杂草的新方法。

8. 发明申请

WO2004067727A3 MIXING AND MATCHING TC PROTEINS FOR PEST CONTROL 审中-公开
公开(公告)号：WO2004067727A3
公开(公告)日：2004-08-12
申请号：PCT/US2004/000394
申请日：2004-01-07
申请人： DOW AGROSCIENCES LLC , HEY, Timothy, D. , SCHLEPER, Amanda, D. , BEVAN, Scott, A. , BINTRIM, Scott, B. , MITCHELL, Jon, C. , LI, Ze, Sheng , NI, Weiting , ZHU, Baolong , MERLO, Donald, J. , APEL-BIRKHOLD, Patricia, C. , MEADE, Thomas
发明人： HEY, Timothy, D. , SCHLEPER, Amanda, D. , BEVAN, Scott, A. , BINTRIM, Scott, B. , MITCHELL, Jon, C. , LI, Ze, Sheng , NI, Weiting , ZHU, Baolong , MERLO, Donald, J. , APEL-BIRKHOLD, Patricia, C. , MEADE, Thomas
IPC分类号： C12N15/82
摘要： The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibacillus, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a "stand-alone" TC protein (from Photorhabdus, Xenorhabdus , or Paenibacillus, for example) is enhanced by one or more TC protein "potentiators" derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand-alone Photorhabdus protein toxin. This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterologous TC proteins are also disclosed herein.

9. 发明申请

WO2008055069A2 POULTRY VACCINE, FOR NECROTIC ENTERITIS, COMPRISING A MUTATED CLOSTRIDIUM PERFRINGENS ALPHA TOXIN ANTIGEN, AND METHODS OF PRODUCING THE VACCINE 审中-公开
标题翻译：家禽疫苗用于包括突变的梭菌α毒素抗原在内的肠型肠病，以及产生疫苗的方法
公开(公告)号：WO2008055069A2
公开(公告)日：2008-05-08
申请号：PCT/US2007/082670
申请日：2007-10-26
申请人： DOW AGROSCIENCES LLC , AINLEY, William, Michael , ARMSTRONG, Janna , MADDURI, Krishna , MERLO, Donald, J. , SMITH, Kelley, A. , THOMPSON, Mark, Allen , WEBB, Steven, Robert , SHEN, Liu, Yin
发明人： AINLEY, William, Michael , ARMSTRONG, Janna , MADDURI, Krishna , MERLO, Donald, J. , SMITH, Kelley, A. , THOMPSON, Mark, Allen , WEBB, Steven, Robert , SHEN, Liu, Yin
IPC分类号： A61K39/08 , C12N15/82 , C12N5/10
CPC分类号： A61K39/08 , A61K2039/552 , A61K2039/55577 , C07K14/33
摘要： This invention pertains in part to the development of a vaccine for poultry against necrotic enteritis (NE). The vaccine utilizes a protective antigen that is a mutated, full-length, non-toxic Clostridium perfringens (Cρ) α-toxin protein (Mcpa). Utility of this vaccine was demonstrated by reduction of lesion severity in NE challenge trails, for example. Also disclosed herein are novel approaches for producing this vaccine in significant quantities. One exemplified approach involves producing NE vaccine (mutated alpha toxin) in bacterial expression systems, preferably utilizing the Pseudomonas fluorescens system, for commercial use in controlling NE in the poultry industry. The subject vaccines can be administered preferably to chickens in several different ways. A novel, Type VI alpha toxin from chicken isolates of Cρ is also disclosed.
摘要翻译：本发明部分涉及针对家禽抵抗坏死性肠炎（NE）的疫苗的开发。该疫苗使用保护性抗原，其是突变的全长无毒产气荚膜梭菌（Cρ）α-毒素蛋白（Mcpa）。例如，通过减少NE挑战迹线中的损伤严重程度来证明该疫苗的实用性。本文还公开了大量生产该疫苗的新方法。一种示例性方法涉及在细菌表达系统中生产NE疫苗（突变的α毒素），优选利用荧光假单胞菌系统，用于商业用于控制家禽业中的NE。主题疫苗可以以几种不同的方式优选施用于鸡。还公开了来自Cp鸡分离物的新型VI型α毒素。

10. 发明申请

WO2008143713A3 PLANT-MADE WEST NILE VIRUS (WNV) VACCINES, VECTORS AND PLANT CODON OPTIMIZED SEQUENCES 审中-公开
公开(公告)号：WO2008143713A3
公开(公告)日：2008-11-27
申请号：PCT/US2007/088512
申请日：2007-12-21
申请人： DOW AGROSCIENCES LLC , THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF AGRICULTURE , SMITH, Kelley, A. , MIHALIAK, Charles, A. , WEBB, Steven, Robert , MERLO, Donald, J. , EVANS, Steven L. , LETCHWORTH, Geoffrey, J.
发明人： SMITH, Kelley, A. , MIHALIAK, Charles, A. , WEBB, Steven, Robert , MERLO, Donald, J. , EVANS, Steven L. , LETCHWORTH, Geoffrey, J.
IPC分类号： C12N15/82 , C07K14/18 , A01H5/00
摘要： The subject application provides various compositions of matter directed to West Nile virus (WNV) polypeptides and fragments thereof and polynucleotides, vectors and transformed host cells that encode, direct the expression of, or produce WNV polypeptides as set forth herein. Methods of using the polypeptides and polynucleotides for the production of immune responses in individuals or detecting the presence of WNV specific or neutralizing antibodies are also provided herein.

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