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1. 发明申请

WO2016012460A1 IMPROVED STRAINS OF AGROBACTERIUM TUMEFACIENS FOR TRANSFERRING DNA INTO PLANTS 审中-公开
标题翻译：将转基因植物转化为植物病毒的改良菌株
公开(公告)号：WO2016012460A1
公开(公告)日：2016-01-28
申请号：PCT/EP2015/066667
申请日：2015-07-21
申请人： RHEINISCHE FRIEDRICH-WILHELMS-UNIVERSITÄT BONN
发明人： ÜLKER, Bekir , BERSON, Tobias
IPC分类号： C12N15/82
CPC分类号： C12N15/743 , C12N15/8205
摘要： The present invention relates to Agrobacterium tumefaciens strains that comprise at least one deletion/mutation in a sequence selected from the group of IS426 copy I, IS426 copy II, the OriT-like sequence, and the border-like sequences, and their uses in safer and improved transformation procedures for cells.
摘要翻译：本发明涉及根癌土壤杆菌菌株，其包含选自IS426拷贝I，IS426拷贝II，OriT样序列和边界样序列的序列中的至少一个缺失/突变，并且其在更安全的用途中并改进细胞转化程序。

2. 发明申请

WO2014060427A2 ELECTROCOMPETENT CELLS AND PREPARATION THEREOF 审中-公开
标题翻译：电化学电池及其制备
公开(公告)号：WO2014060427A2
公开(公告)日：2014-04-24
申请号：PCT/EP2013/071546
申请日：2013-10-15
申请人： UNIVERSITAET DES SAARLANDES , GENE BRIDGES GMBH , TECHNISCHE UNIVERSITAET DRESDEN
发明人： MUELLER, Rolf , TU, Qiang , ZHANG, Youming , STEWART, Francis , FU, Jun
IPC分类号： C12N1/20 , C12N15/87
CPC分类号： C12N15/743 , C12N1/20 , C12N15/70 , C12N15/74
摘要： The present invention provides a method of preparing electrocompetent cells from Gram-negative bacteria characterized in that the bacteria are prepared under room temperature. Also provided are electrocompetent Gram-negative bacteria and kits which comprise the electrocompetent bacteria.
摘要翻译：本发明提供从革兰氏阴性细菌制备电感受态细胞的方法，其特征在于在室温下制备细菌。还提供了电感抗性革兰氏阴性细菌和包含电感受态细菌的试剂盒。

3. 发明申请

WO2012016222A3 STRAINS OF AGROBACTERIUM MODIFIED TO INCREASE PLANT TRANSFORMATION FREQUENCY 审中-公开
标题翻译：农杆菌菌株改良以提高植物转化频率
公开(公告)号：WO2012016222A3
公开(公告)日：2012-06-21
申请号：PCT/US2011046028
申请日：2011-07-29
申请人： DOW AGROSCIENCES LLC , MERLO DONALD J , RUSSELL SEAN M , RETALLACK DIANE , WOOSLEY AARON , MEADE TOM , NARVA KENNETH E
发明人： MERLO DONALD J , RUSSELL SEAN M , RETALLACK DIANE , WOOSLEY AARON , MEADE TOM , NARVA KENNETH E
IPC分类号： A01H5/00 , C12N1/21 , C12N15/82 , C12N15/84
CPC分类号： C12N15/8205 , C07K14/195 , C07K14/325 , C12N15/74 , C12N15/743 , C12N15/8274 , C12N15/8286 , Y02A40/162
摘要： Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.
摘要翻译：提供了在能够独立于农杆菌染色体复制的质粒上携带转化增强基因的农杆菌菌株，Ti质粒和植物转化双元载体，以及这些农杆菌菌株的用途。此外，导致植物转化双元载体不稳定或重排的DNA重组功能缺陷的农杆菌菌株，以及在能够独立于农杆菌染色体，Ti质粒和植物转化二元体复制的质粒上携带转化增强基因载体，以及这些菌株的用途。进一步包括的是农杆菌菌株，其具有整合到农杆菌染色体中的转化增强基因，其不会干扰土壤杆菌细胞的正常生长和植物转化能力或者以其它方式危害土壤杆菌细菌的植物转化能力，以及这些农杆菌菌株的用途。还描述了使用这些农杆菌菌株制造的植物。

4. 发明申请

WO2009114537A1 CHIMERIC PROMOTER MOLECULES FOR GENE EXPRESSION IN PROKARYOTES 审中-公开
标题翻译：用于基因表达的基因启动子分子
公开(公告)号：WO2009114537A1
公开(公告)日：2009-09-17
申请号：PCT/US2009/036676
申请日：2009-03-10
申请人： MONSANTO TECHNOLOGY LLC , YE, Xudong
发明人： YE, Xudong
IPC分类号： C12N15/74
CPC分类号： C12N15/67 , C12N15/743 , C12Q1/68
摘要： The present invention provides regulatory polynucleotide molecules isolated from a 16S rDNA for enhanced expression of heterologous genes. The invention further discloses compositions, polynucleotide constructs, transformed host cells containing the regulatory polynucleotide sequences, and methods for preparing and using the same.
摘要翻译：本发明提供从16S rDNA分离的用于增强异源基因表达的调节性多核苷酸分子。本发明还公开了组合物，多核苷酸构建体，含有调节性多核苷酸序列的转化宿主细胞，以及制备和使用其的方法。

5. 发明申请

WO01064925A1 TRANSFORMATION OF EUKARYOTIC CELLS BY MOBILIZABLE PLASMIDS 审中-公开
标题翻译：通过可动态PLASMIDS转化原核细胞
公开(公告)号：WO01064925A1
公开(公告)日：2001-09-07
申请号：PCT/NL2001/000169
申请日：2001-03-01
IPC分类号： A01H5/00 , C12N1/21 , C12N15/74 , C12N15/80 , C12N15/81 , C12N15/82
CPC分类号： C12N15/81 , C12N15/743 , C12N15/80 , C12N15/8205
摘要： The invention relates to the transfer of genetic material to eukaryotic cells by means of a process resembling conjugation, in particular by a system partially based on Agrobacterium tumefaciens-like transfer systems. In particular transfer of genetic material into plant cells is disclosed using mobilisable, but non-conjugative plasmids by means of an Agrobacterium virulence system. The invention provides a method for transferring genetic material which is not a typical T-DNA surrounded by Agrobacterium T-borders from an Agrobacterium virulence system to an eukaryotic host cell, comprising providing said genetic material on a mobilisable plasmid, capable of forming a relaxosome, bringing said mobilisable plasmid in an Agrobacterium having at least the activity of the transfer genes of Agrobacterium not present on said mobilisable plasmid, whereby the necessary gene products providing the same or similar activity as a functional VirB operon are also present and cocultivating said Agrobacterium with said eukaryotic host cell.
摘要翻译：本发明涉及通过类似于缀合的方法将遗传物质转移到真核细胞中，特别是通过部分基于根癌土壤杆菌样传递系统的系统转运遗传物质。特别是将遗传物质转移到植物细胞中，通过土壤杆菌毒力系统公开了使用可运动但非共轭质粒的方法。本发明提供了一种将不是由农杆菌T型边界包围的典型T-DNA转移到真核宿主细胞的遗传物质的方法，其包括在能够形成松质体的运动性质粒上提供所述遗传物质，将所述可运动质粒携带至至少具有不存在于所述可移动质粒上的农杆菌转移基因活性的农杆菌中，由此提供与功能性VirB操纵子相同或相似活性的必需基因产物，并与所述农杆菌共培养所述农杆菌真核宿主细胞。

6. 发明申请

WO1989008712A1 OXYGEN REGULATABLE GENE EXPRESSION 审中-公开
标题翻译：氧可调节基因表达
公开(公告)号：WO1989008712A1
公开(公告)日：1989-09-21
申请号：PCT/US1989001091
申请日：1989-03-17
申请人： THE GENERAL HOSPITAL CORPORATION
发明人： THE GENERAL HOSPITAL CORPORATION , HUALA, Eva , GU, Quing , ALBRIGHT, Lisa , AUSUBEL, Frederick
IPC分类号： C12P21/00
CPC分类号： C12N15/743 , C07K14/195 , C12N15/70
摘要： The invention discloses that, in the presence of NifA and NtrA, the expression of a desired DNA molecule which has been operably linked to an ntrA-RNA polymerase and NifA dependent promoter can be regulated by regulating the oxygen level of the culture medium. The invention relates to vectors which may be used in accordance with this discovery, and to methods for using such vectors.

7. 发明申请

WO2014009867A1 NEW BACTERIAL STRAINS, PLASMIDS, METHOD OF PRODUCING BACTERIAL STRAINS CAPABLE OF CHEMOLITHOTROPHIC ARSENITES OXIDATION AND USES THEREOF 审中-公开
标题翻译：新菌株菌株，PLASMIDS，生产可溶于亚硝酸盐的细菌菌株的方法氧化及其用途
公开(公告)号：WO2014009867A1
公开(公告)日：2014-01-16
申请号：PCT/IB2013/055577
申请日：2013-07-08
申请人： UNIWERSYTET WARSZAWSKI
发明人： DREWNIAK, Lukasz , SKLODOWSKA, Aleksandra , RADLINSKA, Monika , CIEZKOWSKA, Martyna
IPC分类号： C12N1/00
CPC分类号： C12N15/743 , C02F1/5236 , C02F3/34 , C02F2101/103 , C12N15/52 , C12P3/00 , C12R1/01
摘要： The invention provides novel strains Agrobacterium tumefaciens KKP 2039p and Paracoccus alcaliphilus KKP 2040p, the plasmid pSinA and its functional derivative, method for producing bacterial strains capable of chemolithotrophic arsenite oxidation and novel bacterial strains produced by this method. The invention also relates to the composition, comprising the novel bacterial strain or the plasmid pSinA and the use of these novel strains, as well as the method of bioaugmentation of an arsenic contaminated environment, particularly the method for the removal of arsenic from waters.
摘要翻译：本发明提供新型菌株根癌土壤杆菌KKP 2039p和Alaciphilus KKP 2040p，质粒pSinA及其功能衍生物，用于生产能够通过该方法生产的化学亚硝酸盐氧化的细菌菌株的方法和新的细菌菌株。本发明还涉及包含新型细菌菌株或质粒pSinA以及这些新菌株的用途的组合物，以及砷污染环境的生物强化方法，特别是从水中除去砷的方法。

8. 发明申请

WO2012016222A2 STRAINS OF AGROBACTERIUM MODIFIED TO INCREASE PLANT TRANSFORMATION FREQUENCY 审中-公开
标题翻译：修改农杆菌菌株以增加植物转化频率
公开(公告)号：WO2012016222A2
公开(公告)日：2012-02-02
申请号：PCT/US2011/046028
申请日：2011-07-29
申请人： DOW AGROSCIENCES LLC , MERLO, Donald J. , RUSSELL, Sean M. , RETALLACK, Diane , WOOSLEY, Aaron , MEADE, Tom , NARVA, Kenneth E.
发明人： MERLO, Donald J. , RUSSELL, Sean M. , RETALLACK, Diane , WOOSLEY, Aaron , MEADE, Tom , NARVA, Kenneth E.
IPC分类号： A01H5/00 , C12N15/82 , C12N15/84 , C12N1/21
CPC分类号： C12N15/8205 , C07K14/195 , C07K14/325 , C12N15/74 , C12N15/743 , C12N15/8274 , C12N15/8286 , Y02A40/162
摘要： Agrobacterium strains that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these Agrobacterium strains are provided. Additionally, Agrobacterium strains that are deficient in DNA recombination functions that result in instability or rearrangement of plant transformation binary vectors, and that harbor transformation-enhancing genes on a plasmid capable of replication independently of the Agrobacterium chromosome, the Ti plasmid, and plant transformation binary vectors, and uses for these strains, are also provided. Further included are Agrobacterium strains that harbor transformation-enhancing genes integrated into the Agrobacterium chromosome at a locus that does not interfere with or otherwise compromise the normal growth and plant transformation ability of the Agrobacterium cells, and uses for these Agrobacterium strains. Plants made using these Agrobacterium strains are also described.
摘要翻译：提供了在能够独立于农杆菌染色体，Ti质粒和植物转化二元载体进行复制的质粒上携带转化增强基因的土壤杆菌菌株，以及这些农杆菌菌株的用途。另外，缺乏DNA重组功能的农杆菌菌株导致植物转化双向载体的不稳定或重排，并且能够独立于农杆菌染色体，Ti质粒和植物转化二元体的能够复制的质粒上转录增强基因还提供了这些菌株的载体和用途。还包括农杆菌菌株，其在不干扰或以其他方式损害农杆菌细胞的正常生长和植物转化能力的位点处将整合到农杆菌染色体中的转化增强基因并用于这些农杆菌菌株。还描述了使用这些农杆菌菌株制备的植物。

9. 发明申请

WO2007049416A1 カロテノイド合成微生物の作製方法およびカロテノイドの製造方法审中-公开
标题翻译：用于生产合成微生物的碳氧化合物的方法和用于生产碳酸的方法
公开(公告)号：WO2007049416A1
公开(公告)日：2007-05-03
申请号：PCT/JP2006/318904
申请日：2006-09-25
申请人：東ソー株式会社 , 井出輝彦 , 田中亨
发明人：井出輝彦 , 田中亨
IPC分类号： C12N15/09 , A23K1/16 , A23L1/272 , A23L1/303 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12P23/00
CPC分类号： C12P23/00 , A23K20/179 , A23L5/44 , C12N15/743
摘要： [PROBLEMS] To produce a microorganism capable of producing a carotenoid in an industrial scale. [MEANS FOR SOLVING PROBLEMS] A method for producing a carotenoid comprising the steps of cultivating a cell transformed with a DNA sequence comprising a DNA sequence depicted in any one of SEQ ID NOs:2-7 or a cell transformed with a vector having a DNA sequence depicted in any one of SEQ ID NOs:2-7 under proper culture conditions and isolating the carotenoid from the cell or the culture.
摘要翻译： [问题]生产能够以工业规模生产类胡萝卜素的微生物。解决问题的方法一种类胡萝卜素的制造方法，其特征在于，包括以下步骤：培养用包含SEQ ID NO：2-7中任一项所述的DNA序列的DNA序列转化的细胞或用具有DNA的载体转化的细胞序列SEQ ID NO：2-7中所示的序列，并从细胞或培养物中分离出类胡萝卜素。

10. 发明申请

WO0212519A3 COUNTER SELECTION STRATEGY FOR GRAM-NEGATIVE BACTERIA 审中-公开
标题翻译：灰色阴茎细菌的选择策略
公开(公告)号：WO0212519A3
公开(公告)日：2003-02-06
申请号：PCT/US0141618
申请日：2001-08-07
申请人： UNIV NEBRASKA
发明人： FARRAND STEPHEN K , STASWICK PAUL E , CLEMENTE THOMAS E
IPC分类号： C12N1/21 , C12N15/65 , C12N15/74 , C12N15/82 , C12N9/10 , C12R1/01
CPC分类号： C12N15/8205 , C12N15/65 , C12N15/743
摘要： A Gram-negative bacterium useful for genetically engineering plants is provided. The Gram-negative bacterium contains, as part of genome, an inducible regulatory sequence operatively linked to a nucleotide sequence encoding a levansucrase. Alternatively, the Gram-negative bacterium comprises a recombinant nucleic acid construct containing an inducible regulatory sequence operatively linked to a nucleotide sequence encoding a levansucrase. Also provided are recombinant nucleic acid constructs comprising an inducible regulatory sequence operatively coupled to a nucleotide sequence encoding a levansucrase and a method for transforming plants using the Gram-negative bacterium of the present invention.
摘要翻译：提供了可用于遗传工程植物的革兰氏阴性细菌。作为基因组的一部分，革兰氏阴性细菌含有可操作地连接到编码左聚糖蔗糖酶的核苷酸序列的诱导型调控序列。或者，革兰氏阴性细菌包含重组核酸构建体，其含有可操作地连接于编码左聚糖蔗糖酶的核苷酸序列的诱导型调节序列。还提供了重组核酸构建体，其包含可操作地偶联于编码左聚糖蔗糖酶的核苷酸序列的诱导型调控序列和使用本发明的革兰氏阴性细菌转化植物的方法。

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