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1. 发明申请

WO2004067727A3 MIXING AND MATCHING TC PROTEINS FOR PEST CONTROL 审中-公开
公开(公告)号：WO2004067727A3
公开(公告)日：2004-08-12
申请号：PCT/US2004/000394
申请日：2004-01-07
申请人： DOW AGROSCIENCES LLC , HEY, Timothy, D. , SCHLEPER, Amanda, D. , BEVAN, Scott, A. , BINTRIM, Scott, B. , MITCHELL, Jon, C. , LI, Ze, Sheng , NI, Weiting , ZHU, Baolong , MERLO, Donald, J. , APEL-BIRKHOLD, Patricia, C. , MEADE, Thomas
发明人： HEY, Timothy, D. , SCHLEPER, Amanda, D. , BEVAN, Scott, A. , BINTRIM, Scott, B. , MITCHELL, Jon, C. , LI, Ze, Sheng , NI, Weiting , ZHU, Baolong , MERLO, Donald, J. , APEL-BIRKHOLD, Patricia, C. , MEADE, Thomas
IPC分类号： C12N15/82
摘要： The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibacillus, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a "stand-alone" TC protein (from Photorhabdus, Xenorhabdus , or Paenibacillus, for example) is enhanced by one or more TC protein "potentiators" derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand-alone Photorhabdus protein toxin. This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterologous TC proteins are also disclosed herein.

2. 发明申请

WO2004067750A3 XENORHABDUS TC PROTEINS AND GENES FOR PEST CONTROL 审中-公开
标题翻译： XENORHABDUS TC蛋白和基因用于虫害控制
公开(公告)号：WO2004067750A3
公开(公告)日：2004-10-07
申请号：PCT/US2004000314
申请日：2004-01-07
申请人： DOW AGROSCIENCES LLC , BINTRIM SCOTT B , MITCHELL JON C , LARRINUA IGNACIO M , APEL-BIRKHOLD PATRICIA C , GREEN SUSAN B , SCHAFER BARRY W , BEVAN SCOTT A , YOUNG SCOTT A , GUO LINING
发明人： BINTRIM SCOTT B , MITCHELL JON C , LARRINUA IGNACIO M , APEL-BIRKHOLD PATRICIA C , GREEN SUSAN B , SCHAFER BARRY W , BEVAN SCOTT A , YOUNG SCOTT A , GUO LINING
IPC分类号： C12N5/02 , C12N15/82 , C07K14/24
CPC分类号： C12N15/8286 , Y02A40/162
摘要： The subject invention relates to novel Xenorhabdus toxin complex (TC) proteins and genes that encode these proteins. More specifically, the subject invention relates to TC genes and proteins obtainable from Xenorhabdus strain Xwi. The subject invention also provides an exochitinase obtainable from the Xwi strain. This exochitinase can be used to control insects using methods known in the art.
摘要翻译：本发明涉及新的Xenorhabdus毒素复合物（TC）蛋白质和编码这些蛋白质的基因。更具体地说，本发明涉及可从Xenorhabdus菌株Xwi获得的TC基因和蛋白质。本发明还提供可从Xwi菌株获得的外切壳多糖酶。该外切壳多糖酶可用于使用本领域已知的方法来控制昆虫。

3. 发明申请

WO2004067727A2 MIXING AND MATCHING TC PROTEINS FOR PEST CONTROL 审中-公开
标题翻译：混合和匹配TC蛋白进行病虫害防治
公开(公告)号：WO2004067727A2
公开(公告)日：2004-08-12
申请号：PCT/US2004000394
申请日：2004-01-07
申请人： DOW AGROSCIENCES LLC , HEY TIMOTHY D , SCHLEPER AMANDA D , BEVAN SCOTT A , BINTRIM SCOTT B , MITCHELL JON C , LI ZE SHENG , NI WEITING , ZHU BAOLONG , MERLO DONALD J , APEL-BIRKHOLD PATRICIA C
发明人： HEY TIMOTHY D , SCHLEPER AMANDA D , BEVAN SCOTT A , BINTRIM SCOTT B , MITCHELL JON C , LI ZE SHENG , NI WEITING , ZHU BAOLONG , MERLO DONALD J , APEL-BIRKHOLD PATRICIA C , MEADE THOMAS
IPC分类号： A01N37/46 , A01N63/02 , C07K14/195 , C07K14/24 , C12N15/82 , C12N
CPC分类号： C07K14/195 , A01N37/46 , A01N63/02 , C07K14/24 , C12N15/8286 , Y02A40/162
摘要： The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibucillus, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a "stand-alone" TC protein (from Photorhabdus, Xenorhabdus, or Paenibacillus, for example) is enhanced by one or more TC protein "potentiators" derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will new be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand alone Photorlzabdus protein toxin. (As one skilled in the art knows, Xenorhabdus toxin proteins tend to be more desirable for controlling lepidopterans while Photorhabdus toxin proteins tend to be more desirable for controlling coleopterans.) This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterolugous TC proteins are also disclosed herein. Other objects, advantages, and features of the subject invention will be apparent to cue skilled in the art having the benefit of the subject disclosure.
摘要翻译：本发明涉及令人惊奇的发现，可以从Xenorhabdus，Photorhabdus和Paenibucillus获得的毒素复合物（TC）蛋白质彼此互换使用。在本发明的特别优选的实施方案中，“独立”TC蛋白（例如，来自Photorhabdus，Xenorhabdus或Paenibacillus的例如）的毒性由一种或多种衍生自以下的源生物体的TC蛋白“增强剂”增强来源于毒素的不同属。本领域技术人员将会认识到本公开的益处，这具有广泛的含义，并且扩展了新认证的各种类型的TC蛋白质具有的效用范围。最重要的优点之一是，本领域技术人员现在将能够使用单组增效剂来增强独立的Xenorhabdus蛋白毒素的活性以及独立的Photorlzabdus蛋白毒素。（如本领域技术人员所知，Xenorhabdus毒素蛋白质倾向于更有利于控制鳞翅目，而Photorhabdus毒素蛋白质倾向于更有利于控制鞘翅目）。这减少了需要表达的基因数量和转化事件一个转基因植物，以实现更广泛的目标害虫的有效控制。杂种TC蛋白的某些优选组合也在本文中公开。本发明的其它目的，优点和特征对本领域的技术人员而言是显而易见的。

4. 发明申请

WO2004067727A8 MIXING AND MATCHING TC PROTEINS FOR PEST CONTROL 审中-公开
标题翻译：混合和匹配TC蛋白进行病虫害防治
公开(公告)号：WO2004067727A8
公开(公告)日：2005-08-18
申请号：PCT/US2004000394
申请日：2004-01-07
申请人： DOW AGROSCIENCES LLC , HEY TIMOTHY D , SCHLEPER AMANDA D , BEVAN SCOTT A , BINTRIM SCOTT B , MITCHELL JON C , LI ZE SHENG , NI WEITING , ZHU BAOLONG , MERLO DONALD J , APEL-BIRKHOLD PATRICIA C , MEADE THOMAS
发明人： HEY TIMOTHY D , SCHLEPER AMANDA D , BEVAN SCOTT A , BINTRIM SCOTT B , MITCHELL JON C , LI ZE SHENG , NI WEITING , ZHU BAOLONG , MERLO DONALD J , APEL-BIRKHOLD PATRICIA C , MEADE THOMAS
IPC分类号： A01N37/46 , A01N63/02 , C07K14/195 , C07K14/24 , C12N15/82 , A01H5/00 , C12N5/10
CPC分类号： C07K14/195 , A01N37/46 , A01N63/02 , C07K14/24 , C12N15/8286 , Y02A40/162
摘要： The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibacillus, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a "stand-alone" TC protein (from Photorhabdus, Xenorhabdus, or Paenibacillus, for example) is enhanced by one or more TC protein "potentiators" derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand-alone Photorhabdus protein toxin. This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterologous TC proteins are also disclosed herein.
摘要翻译：本发明涉及令人惊讶的发现，即可从Xenorhabdus，光杆状芽孢杆菌和类芽孢杆菌中获得的毒素复合物（TC）蛋白可彼此互换使用。在本发明的特别优选的实施方案中，“独立的”TC蛋白（例如来自光杆状病毒，Xenorhabdus或类芽孢杆菌属）的毒性通过一种或多种衍生自一种或多种来源于源生物的TC蛋白“增效剂” 毒素来自的不同属。如本领域技术人员将认识到本公开的益处，这具有广泛的含义并扩大了各种类型的TC蛋白现在将被识别具有的效用范围。其中最重要的优点是本领域技术人员现在将能够使用单组增效剂来增强独立的致病杆菌蛋白毒素以及独立的光杆状菌蛋白毒素的活性。这减少了转基因植物需要表达的基因数量和转化事件，以实现对更广谱目标害虫的有效控制。本文还公开了异源TC蛋白的某些优选组合。

5. 发明申请

WO2004067750A2 XENORHABDUS TC PROTEINS AND GENES FOR PEST CONTROL 审中-公开
标题翻译： XENORHABDUS TC蛋白和基因用于病虫害控制
公开(公告)号：WO2004067750A2
公开(公告)日：2004-08-12
申请号：PCT/US2004/000314
申请日：2004-01-07
申请人： DOW AGROSCIENCES LLC , BINTRIM, Scott, B. , MITCHELL, Jon, C. , LARRINUA, Ignacio, M. , APEL-BIRKHOLD, Patricia, C. , GREEN, Susan, B. , SCHAFER, Barry, W. , BEVAN, Scott, A. , YOUNG, Scott, A. , GUO, Lining
发明人： BINTRIM, Scott, B. , MITCHELL, Jon, C. , LARRINUA, Ignacio, M. , APEL-BIRKHOLD, Patricia, C. , GREEN, Susan, B. , SCHAFER, Barry, W. , BEVAN, Scott, A. , YOUNG, Scott, A. , GUO, Lining
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , Y02A40/162
摘要： The subject invention relates to novel Xenorhabdus toxin complex (TC) proteins and genes that encode these proteins. More specifically, the subject invention relates to TC genes and proteins obtainable from Xenorhabdus strain Xwi. The subject invention also provides an exochitinase obtainable from the Xwi strain. This exochitinase can be used to control insects using methods known in the art.
摘要翻译：本发明涉及新型异戊酰梭菌毒素复合物（TC）蛋白质和编码这些蛋白质的基因。更具体地，本发明涉及从Xenorhabdus菌株Xwi获得的TC基因和蛋白质。本发明还提供可从Xwi菌株获得的外切蛋白酶。该外切蛋白酶可以使用本领域已知的方法来控制昆虫。

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