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1. 发明申请

WO2016028212A1 GENETICALLY MODIFIED PLANTS HAVING ALTERED LIGNIN CONTENT 审中-公开
标题翻译：具有改变的木质素含量的遗传修饰植物
公开(公告)号：WO2016028212A1
公开(公告)日：2016-02-25
申请号：PCT/SE2015/050884
申请日：2015-08-20
申请人： SWETREE TECHNOLOGIES AB , VIB VZW , UNIVERSITEIT GENT
发明人： BOERJAN, Wout , VANHOLME, Ruben , TUOMINEN, Hannele , TURUMTAY, Halbay , ESCAMEZ, Sacha , ZHANG, Bo
IPC分类号： C12N15/82 , A01H1/00 , A01H5/00 , A01H7/00
CPC分类号： C12N15/8255 , C07K14/415
摘要： The invention relates to methods for producing a plant with altered lignin content by genetically manipulating expression of a PIRIN gene. The said method comprises (a) altering expression of a gene encoding a PIRIN2 polypeptide in the plant; and/or (b) altering expression of a gene encoding a PIRIN4 polypeptide in the plant. A plant with reduced lignin content is produced by increasing expression of a gene encoding a PIRIN2 polypeptide in the plant or decreasing expression of a gene encoding a PIRIN4 polypeptide in the plant. The invention further relates to genetically modified plants produced by the said methods.
摘要翻译：本发明涉及通过遗传操纵PIRIN基因的表达来生产具有改变的木质素含量的植物的方法。所述方法包括（a）改变植物中编码PIRIN2多肽的基因的表达; 和/或（b）改变植物中编码PIRIN4多肽的基因的表达。通过增加植物中编码PIRIN2多肽的基因的表达或减少植物中编码PIRIN4多肽的基因的表达来产生具有降低的木质素含量的植物。本发明还涉及通过所述方法生产的转基因植物。

2. 发明申请

WO2015059661A1 METHOD FOR INCREASING CELLULOSE IN SUGARCANE 审中-公开
标题翻译：用于增加糖的纤维素的方法
公开(公告)号：WO2015059661A1
公开(公告)日：2015-04-30
申请号：PCT/IB2014/065564
申请日：2014-10-23
申请人： STELLENBOSCH UNIVERSITY
发明人： KOSSMANN, Jens Matthias , NDIMANDE, Gordon Sandile , BEKKER, Jan Pieter Ignatius
IPC分类号： C12N15/82 , C07K14/435
CPC分类号： C12N15/8246 , C12N9/1059 , C12N15/8255 , C12P7/10 , C12P19/14 , C12Y204/01012 , Y02E50/16
摘要： A method for increasing the cellulose and/or glucose content of sugarcane is described, wherein a heterologous polypeptide encoding a cellulose synthase having an amino acid sequence which is at least 70% identical to SEQ ID NO: 1 is expressed in the sugarcane. The modified sugarcane can also be used to produce a biofuel.
摘要翻译：描述了增加甘蔗纤维素和/或葡萄糖含量的方法，其中编码具有与SEQ ID NO：1至少70％相同的氨基酸序列的纤维素合成酶的异源多肽在甘蔗中表达。改性甘蔗也可用于生产生物燃料。

3. 发明申请

WO2014004683A3 METHODS AND COMPOSITION FOR ENHANCED FORAGE QUALITY 审中-公开
标题翻译：加强质量的方法和组成
公开(公告)号：WO2014004683A3
公开(公告)日：2014-04-24
申请号：PCT/US2013047911
申请日：2013-06-26
申请人： MONSANTO TECHNOLOGY LLC , FORAGE GENETICS INTERNATIONAL LLC
发明人： HIATT WILLIAM , REDDY MARRY S , MCCASLIN MARK , TEMPLE STEPHEN , WHALEN DAVID , CERNY RICHARD ERIC
IPC分类号： C12N15/82 , A01H1/04 , A01H5/04
CPC分类号： C12N15/8255 , C12N15/8218 , C12Q1/6895 , C12Q2600/13 , G01N33/0098
摘要： The invention provides a method to select an alfalfa plant with enhanced feed value and increased flexibility in management either to increase quality of forage provided or to enhance biomass available earlier.
摘要翻译：本发明提供了选择具有增强的饲料价值和增加管理灵活性的苜蓿植物的方法，以增加所提供的饲料的质量或增强早期提供的生物量。

4. 发明申请

WO2014033714A1 ISOLATED POLYNUCLEOTIDES, POLYPEPTIDES AND METHODS OF USING SAME FOR INCREASING ABIOTIC STRESS TOLERANCE, BIOMASS AND YIELD OF PLANTS 审中-公开
标题翻译：分离的多核苷酸，多聚体和使用它们的方法增加生物胁迫耐受性，生物量和产量
公开(公告)号：WO2014033714A1
公开(公告)日：2014-03-06
申请号：PCT/IL2013/050725
申请日：2013-08-26
申请人： EVOGENE LTD.
发明人： DANGOOR, Inbal Nurith , KARCHI, Hagai
IPC分类号： A01H5/00 , C12N15/82 , C12N15/29 , C07K14/415
CPC分类号： C12N15/8271 , C07K14/415 , C12N15/8247 , C12N15/8255 , C12N15/8261 , C12N15/8273 , Y02A40/146
摘要： Provided are isolated polypeptides which are at least 80% homologous to SEQ ID NOs: 529, 475-528, 530-770, 6179-9796, 9798-10421, isolated polynucleotides which are at least 80% identical to SEQ ID NOs: 314, 1-313, 315-474, 771-6178, nucleic acid constructs comprising same, transgenic cells expressing same, transgenic plants expressing same and method of using same for increasing abiotic stress tolerance, yield, growth rate, biomass, vigor, oil content, photosynthetic capacity, seed yield, fiber yield, fiber quality, fiber length, and/or nitrogen use efficiency of a plant.
摘要翻译：提供与SEQ ID NO：529,475-528,530-770,6179-9796,9798-10421至少80％同源的分离的多肽，与SEQ ID NO：314至少80％相同的分离的多核苷酸， 1-313,315-474,771-6178，包含其的核酸构建体，表达相同的转基因细胞，表达相同的转基因植物及其用于增加非生物胁迫耐受性，产量，生长速率，生物量，活力，油含量，植物的光合能力，种子产量，纤维产量，纤维质量，纤维长度和/或氮利用效率。

5. 发明申请

WO2013007848A3 METHOD FOR INCREASING OR DECREASING THE DEVELOPMENT OF SYLLEPTIC OR PROLEPTIC BRANCHING IN A LIGNEOUS PLANT 审中-公开
标题翻译：增加或减少高等植物中SY枝或枝条分枝发育的方法
公开(公告)号：WO2013007848A3
公开(公告)日：2013-08-01
申请号：PCT/ES2012070471
申请日：2012-06-26
申请人： UNIV MADRID POLITECNICA , ALLONA ALBERICH ISABEL , MORENO CORTES ALICIA , ARAGONCILLO BALLESTEROS CIPRIANO
发明人： ALLONA ALBERICH ISABEL , MORENO CORTES ALICIA , ARAGONCILLO BALLESTEROS CIPRIANO
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8255 , C07K14/415 , C12N15/8218 , C12N15/8261 , Y02A40/146
摘要： The invention relates to a biotechnological application of the RAV1 (Related to ABI3 and Viviparous 1) gene and the RAV2 counterpartthereof in relation to the capacity thereof, when the expression levels thereof or the activity of the proteins that they encode are modified, to increase or decrease the development of sylleptic and/or proleptic branches in ligneous species. The modification of the expression of said genes enables an increase in the production of biomass of a plantation of ligneous species, or a reduction in the number of knots in the trunk of ligneous species for use as timber.
摘要翻译：本发明涉及当它们的表达水平或它们编码的蛋白质的活性被修饰时RAV1（与ABI3和胎盘1相关）基因及其RAV2对应物的生物技术应用相对于其容量的增加或减少木质物种中sylleptic和/或proleptic分支的发展。所述基因表达的修饰能够增加木质植物种植的生物量的产量，或减少用作木材的木质物种树干的结的数量。

6. 发明申请

WO2013106823A1 MATERIALS, SYSTEMS, ORGANISMS, AND METHODS FOR ENHANCING ABIOTIC STRESS TOLERANCE, INCREASING BIOMASS, AND/OR ALTERING LIGNIN COMPOSITION 审中-公开
标题翻译：材料，系统，有机体和增强生物量的方法，提高生物量和/或改变木质素组成
公开(公告)号：WO2013106823A1
公开(公告)日：2013-07-18
申请号：PCT/US2013/021432
申请日：2013-01-14
申请人： THE TEXAS A&M UNIVERSITY SYSTEM
发明人： MIRKOV, T. Erik , BEYENE, Getu , DAMAJ, Mona
IPC分类号： A01H5/00 , C12N15/63 , C12N15/29 , C12N15/82
CPC分类号： C12N15/8273 , C07K14/415 , C12N15/8218 , C12N15/8222 , C12N15/8237 , C12N15/8246 , C12N15/8255 , C12N15/8261 , C12N15/8297 , Y02A40/146
摘要： The present disclosure relates, in some embodiments, to materials, systems, organisms, and methods for enhancing abiotic stress tolerance (e.g., cold, salinity, drought, wind), increasing biomass, and/or altering lignin composition in plants. For example, enhancing abiotic stress tolerance may be achieved using a plant-specific family of transcription factors is APETALA2 (AP2), that includes c-repeat binding factor (e.g., CBF1, CBF3) and AP37 nucleic acids and/or polypeptides. In some embodiments, increasing biomass may be achieved by altering expression of gibberellin oxidases (e.g., GA3ox3/GA2ox4) nucleic acids and/or polypeptides. Altering lignin composition may be achieved by suppression of stem-thickening in pith (e.g., STP1) nucleic acids and/or polypeptides.
摘要翻译：在一些实施方案中，本公开涉及用于增强非生物胁迫耐受性（例如，冷，盐度，干旱，风），增加生物质和/或改变植物中木质素组成的材料，系统，生物体和方法。例如，可以使用植物特异性转录因子家族APETALA2（AP2）（其包括c重复结合因子（例如CBF1，CBF3）和AP37核酸和/或多肽）来实现增强非生物胁迫耐受性。在一些实施方案中，可通过改变赤霉素氧化酶（例如GA3ox3 / GA2ox4）核酸和/或多肽的表达来实现增加的生物量。改变木质素组合物可以通过抑制髓（例如STP1）核酸和/或多肽中的茎增稠来实现。

7. 发明申请

WO2013040572A2 COMPOSITIONS AND METHODS FOR THE PRODUCTION AND USE OF HUMAN CHOLINESTERASES 审中-公开
标题翻译：用于生产和使用人胆碱酯酶的组合物和方法
公开(公告)号：WO2013040572A2
公开(公告)日：2013-03-21
申请号：PCT/US2012055781
申请日：2012-09-17
申请人： UNIV ARIZONA , MOR TSAFRIR , KANNAN LATHA , LARRIMORE KATHERINE E
发明人： MOR TSAFRIR , KANNAN LATHA , LARRIMORE KATHERINE E
IPC分类号： C12N15/83 , C12N9/16 , C12N15/29 , C12N15/55
CPC分类号： C12N9/18 , C12N15/8255 , C12N15/8257 , C12Y301/01008
摘要： In some aspects, the present invention relates to compositions and methods for the production of human cholinesterases. More particularly, it relates to methods for the production of human cholinesterases using transient expression and vectors for producing the same. In one aspect, the present invention relates to a plant viral vector encoding a plant codon-optimized DNA sequence that results in accumulation of the cholinesterase in a plant leaf at levels greater than 20 mg, and in some embodiments greater than 200 mg, of the enzyme per kilogram of the plant leaf.
摘要翻译：在一些方面，本发明涉及用于生产人胆碱酯酶的组合物和方法。更具体地说，涉及使用瞬时表达产生人类胆碱酯酶的方法以及用于生产人类胆碱酯酶的载体。在一个方面，本发明涉及编码植物密码子优化的DNA序列的植物病毒载体，其导致胆碱酯酶在植物叶中以大于20mg，并且在一些实施方案中大于200mg的水平酶每千克植物叶子。

8. 发明申请

WO2012143805A1 METHODS OF MODIFYING LIGNIN BIOSYNTHESIS AND IMPROVING DIGESTIBILITY 审中-公开
标题翻译：修饰木质素生物合成和改善可溶性的方法
公开(公告)号：WO2012143805A1
公开(公告)日：2012-10-26
申请号：PCT/IB2012/051503
申请日：2012-03-29
申请人： BASF PLANT SCIENCE COMPANY GMBH , SEO, Beomseok , GUAN, Hanping , REN, Peifeng , DEPPONG, David , FU, Huihua , BASF (CHINA) COMPANY LIMITED
发明人： SEO, Beomseok , GUAN, Hanping , REN, Peifeng , DEPPONG, David , FU, Huihua
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N9/1011 , C12N15/8255 , C12Y201/01104
摘要： Provided are methods for increasing digestibility and/or crude protein of a plant by modifying lignin biosynthesis of the plant. The methods involve the manipulation of the expression level of genes in the lignin biosynthesis pathway by directly reducing the expression of genes using miRNA or using regulation of transcription factors. Expression cassettes for achieving such gene expression manipulation and transgenic plant cells and plants comprising the constructs and cassettes are also provided. Transcription regulating nucleotide sequences for use in the expression cassettes have also been developed as well as methods of using these sequences and cassettes.
摘要翻译：提供了通过改变植物的木质素生物合成来增加植物的消化率和/或粗蛋白质的方法。该方法涉及通过使用miRNA直接降低基因的表达或使用转录因子的调节来操纵木质素生物合成途径中基因的表达水平。还提供了用于实现这种基因表达操纵的表达盒和包含构建体和盒的转基因植物细胞和植物。还开发了用于表达盒的转录调节核苷酸序列以及使用这些序列和盒的方法。

9. 发明申请

WO2012141568A1 A METHOD OF REGULATING LIGNIN CONTENT IN THE PLANT OF HEVEA BRASILIENSIS 审中-公开
标题翻译：一种调节血管内皮细胞生长因子含量的方法
公开(公告)号：WO2012141568A1
公开(公告)日：2012-10-18
申请号：PCT/MY2012/000011
申请日：2012-01-30
申请人： UNIVERSITI SAINS MALAYSIA , ALAM, Maqsudul , NAJIMUDIN, Mohd, Nazalan, Mohd , SAITO, Jennifer, Ann , USHARRAJ, Abhilash, Othalathara
发明人： ALAM, Maqsudul , NAJIMUDIN, Mohd, Nazalan, Mohd , SAITO, Jennifer, Ann , USHARRAJ, Abhilash, Othalathara
IPC分类号： C12N9/02 , C07K14/415 , C12N15/53 , A01H1/00 , C12N15/29 , C12N15/82
CPC分类号： C12N15/8255 , C12N9/0006
摘要： A method of reducing production of lignin content in the plant of Hevea brasiliensis comprises the step of down-regulating expression of at least one nucleic acid template containing sequence SEQ ID No.l, SEQ ID No.2, SEQ ID No.3 and SEQ ID No.4 in at least one cell.
摘要翻译：降低巴西三叶草植物中木质素含量产生的方法包括下调至少一种含有序列SEQ ID No.1，SEQ ID No.2，SEQ ID No.3和SEQ ID No.3的核酸模板的表达的步骤至少一个单元格中的ID号4。

10. 发明申请

WO2011160050A3 SYSTEMS TO REDUCE RECALCITRANCE OF CELLULOSIC BIOMASS AND INCREASE YIELDS OF FERMENTABLE SUGARS 审中-公开
标题翻译：减少细胞生物量回收和增加可食用糖的系统
公开(公告)号：WO2011160050A3
公开(公告)日：2012-06-28
申请号：PCT/US2011040928
申请日：2011-06-17
申请人： EDENSPACE SYSTEMS CORP , PAPPAN KIRK , BLAYLOCK MICHAEL , LEE DAVID , FERGUSON BRUCE
发明人： PAPPAN KIRK , BLAYLOCK MICHAEL , LEE DAVID , FERGUSON BRUCE
IPC分类号： A01H5/00 , C12N15/55 , C12N15/82 , C12P19/12
CPC分类号： C12N9/00 , C12N9/18 , C12N9/2437 , C12N15/8255 , C12P19/02 , C12P19/14 , C12P2201/00 , C12Y302/01091
摘要： Described herein are methods to reduce the resistance of the plant cell wall to hydrolysis, methods to enhance the conversion of plant biomass into fermentable sugars, methods to increase yields of fermentable sugars from corn grain hydrolysis, and techniques to harvest both the grain and stover from the field simultaneously for hydrolysis. In certain embodiments, the invention provides transgenic plants transformed with expression vectors containing a DNA sequence encoding plant cell wall-modifying enzymes from microbial and animal sources. In certain embodiments, the invention provides methods of improving enzyme performance by creating variants of the parental enzyme. In certain embodiments, the invention provides methods of using stay-green varieties as biomass feedstocks for cellulosic biofuel production. Uses of this invention include, but are not limited to, enhanced biomass conversion, cost-effective lignocellulosic biomass conversion and biofuel production, reduced biomass recalcitrance, enhanced forage digestibility by animals, improved fiber qualities, and enhanced fiber processing properties.
摘要翻译：本文描述的是减少植物细胞壁对水解的抗性的方法，增强植物生物质转化为可发酵糖的方法，从玉米粒水解中提高可发酵糖产量的方法，以及收获谷物和秸秆两者的技术该领域同时进行水解。在某些实施方案中，本发明提供用含有编码来自微生物和动物来源的植物细胞壁修饰酶的DNA序列的表达载体转化的转基因植物。在某些实施方案中，本发明提供了通过产生亲本酶的变体来提高酶性能的方法。在某些实施方案中，本发明提供了使用保留绿色品种作为纤维素生物燃料生产的生物质原料的方法。本发明的用途包括但不限于增强的生物质转化，成本有效的木质纤维素生物质转化和生物燃料生产，降低的生物质耐受性，动物提高的饲料消化率，改善的纤维品质和增强的纤维加工性能。

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