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1. 发明申请

WO2004067727A3 MIXING AND MATCHING TC PROTEINS FOR PEST CONTROL 审中-公开
公开(公告)号：WO2004067727A3
公开(公告)日：2004-08-12
申请号：PCT/US2004/000394
申请日：2004-01-07
申请人： DOW AGROSCIENCES LLC , HEY, Timothy, D. , SCHLEPER, Amanda, D. , BEVAN, Scott, A. , BINTRIM, Scott, B. , MITCHELL, Jon, C. , LI, Ze, Sheng , NI, Weiting , ZHU, Baolong , MERLO, Donald, J. , APEL-BIRKHOLD, Patricia, C. , MEADE, Thomas
发明人： HEY, Timothy, D. , SCHLEPER, Amanda, D. , BEVAN, Scott, A. , BINTRIM, Scott, B. , MITCHELL, Jon, C. , LI, Ze, Sheng , NI, Weiting , ZHU, Baolong , MERLO, Donald, J. , APEL-BIRKHOLD, Patricia, C. , MEADE, Thomas
IPC分类号： C12N15/82
摘要： The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibacillus, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a "stand-alone" TC protein (from Photorhabdus, Xenorhabdus , or Paenibacillus, for example) is enhanced by one or more TC protein "potentiators" derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand-alone Photorhabdus protein toxin. This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterologous TC proteins are also disclosed herein.

2. 发明申请

WO2004067750A2 XENORHABDUS TC PROTEINS AND GENES FOR PEST CONTROL 审中-公开
标题翻译： XENORHABDUS TC蛋白和基因用于病虫害控制
公开(公告)号：WO2004067750A2
公开(公告)日：2004-08-12
申请号：PCT/US2004/000314
申请日：2004-01-07
申请人： DOW AGROSCIENCES LLC , BINTRIM, Scott, B. , MITCHELL, Jon, C. , LARRINUA, Ignacio, M. , APEL-BIRKHOLD, Patricia, C. , GREEN, Susan, B. , SCHAFER, Barry, W. , BEVAN, Scott, A. , YOUNG, Scott, A. , GUO, Lining
发明人： BINTRIM, Scott, B. , MITCHELL, Jon, C. , LARRINUA, Ignacio, M. , APEL-BIRKHOLD, Patricia, C. , GREEN, Susan, B. , SCHAFER, Barry, W. , BEVAN, Scott, A. , YOUNG, Scott, A. , GUO, Lining
IPC分类号： C12N15/82
CPC分类号： C12N15/8286 , Y02A40/162
摘要： The subject invention relates to novel Xenorhabdus toxin complex (TC) proteins and genes that encode these proteins. More specifically, the subject invention relates to TC genes and proteins obtainable from Xenorhabdus strain Xwi. The subject invention also provides an exochitinase obtainable from the Xwi strain. This exochitinase can be used to control insects using methods known in the art.
摘要翻译：本发明涉及新型异戊酰梭菌毒素复合物（TC）蛋白质和编码这些蛋白质的基因。更具体地，本发明涉及从Xenorhabdus菌株Xwi获得的TC基因和蛋白质。本发明还提供可从Xwi菌株获得的外切蛋白酶。该外切蛋白酶可以使用本领域已知的方法来控制昆虫。

3. 发明申请

WO2004002223A2 PESTICIDALLY ACTIVE PROTEINS AND POLYNUCLEOTIDES OBTAINABLE FROM PAENIBACILLUS SPECIES 审中-公开
标题翻译：农药活性蛋白和可从PAENIBACILLUS物种获得的多核苷酸
公开(公告)号：WO2004002223A2
公开(公告)日：2004-01-08
申请号：PCT/US2003/020082
申请日：2003-06-27
申请人： DOW AGROSCIENCES LLC
发明人： BINTRIM, Scott, B. , BEVAN, Scott, A. , ZHU, Baolong , MERLO, Donald, J.
IPC分类号： A01N
CPC分类号： C12P19/34 , C07K14/195 , C12P21/02 , C12Q1/689 , C12Q2600/142
摘要： The subject invention provides unique biological alternatives for pest control. More specifically, the present invention relates to novel pesticidal proteins, novel sources of pesticidal proteins, polynucleotides that encode such toxins, and to methods of using these toxins to control insects and other plant pests. The subject invention relates to the surprising discovery that Paenibacillus species, and proteins therefrom, have toxicity to lepidopterans. There have been no known reports of a Paenibacillus species, strain, or protein having toxicity to lepidopterans. This is also the first known example of a Paenibacillus Cry protein that is toxic to lepidopterans. Furthermore, this is the first known report of Paenibacillus having toxin complex (TC)-like proteins. The DAS1529 isolate disclosed here is also the first known example of a natural bacterium that produces both a Cry toxin and TC proteins. The subject invention also relates to new classes of Cry and TC proteins that are pesticidally active.
摘要翻译：本发明提供了用于病虫害防治的独特的生物学替代物。更具体地，本发明涉及新型杀虫蛋白，杀虫蛋白的新来源，编码这种毒素的多核苷酸，以及使用这些毒素来控制昆虫和其它植物害虫的方法。本发明涉及令人惊奇的发现：Paenibacillus物种及其蛋白质对鳞翅目病毒具有毒性。没有已知的具有对鳞翅目昆虫具有毒性的Paenibacillus物种，菌株或蛋白质的报道。这也是对鳞翅目昆虫具有毒性的第一个已知的Paenibacillus Cry蛋白的例子。此外，这是具有毒素复合物（TC）样蛋白质的Paenibacillus的第一个已知报告。此处公开的DAS1529分离物也是产生Cry毒素和TC蛋白质的天然细菌的第一已知实例。本发明还涉及具有杀虫活性的新类型的Cry和TC蛋白。

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