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11. 发明申请

WO1993008278A1 PEPTIDE LIBRARY AND SCREENING METHOD 审中-公开
标题翻译：肽库和筛选方法
公开(公告)号：WO1993008278A1
公开(公告)日：1993-04-29
申请号：PCT/US1992008879
申请日：1992-10-15
申请人： AFFYMAX TECHNOLOGIES N.V. , SCHATZ, Peter, J. , CULL, Millard, G. , MILLER, Jeff, F. , STEMMER, Willem, Peter, Christian
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C12N15/10
CPC分类号： G01N33/68 , C12N15/1075
摘要： A random peptide library constructed by transforming host cells with a collection of recombinant vectors that encode a fusion protein comprised of a DNA binding protein and a random peptide and also contain a binding site for the DNA binding protein can be used to screen for novel ligands. The screening method results in the formation of a complex comprising the fusion protein bound to a receptor through the random peptide ligand and to the recombinant DNA vector through the DNA binding protein.

12. 发明申请

WO1990015070A1 VERY LARGE SCALE IMMOBILIZED PEPTIDE SYNTHESIS 审中-公开
标题翻译：非常大的规模固定肽合成
公开(公告)号：WO1990015070A1
公开(公告)日：1990-12-13
申请号：PCT/NL1990000081
申请日：1990-06-07
申请人： AFFYMAX TECHNOLOGIES N.V.
发明人： AFFYMAX TECHNOLOGIES N.V. , PIRRUNG, Michael, C. , READ, J., Leighton , FODOR, Stephen, P., A. , STRYER, Lubert
IPC分类号： C07K01/04
CPC分类号： C08G69/00 , B01J19/0046 , B01J2219/00315 , B01J2219/00432 , B01J2219/00434 , B01J2219/00436 , B01J2219/00459 , B01J2219/00468 , B01J2219/00475 , B01J2219/005 , B01J2219/00527 , B01J2219/00529 , B01J2219/00531 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00639 , B01J2219/00648 , B01J2219/00659 , B01J2219/00689 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01L7/52 , B82Y10/00 , B82Y30/00 , C07B2200/11 , C07C229/14 , C07C229/16 , C07D263/44 , C07D317/62 , C07H19/04 , C07H19/10 , C07H21/00 , C07K1/04 , C07K1/042 , C07K1/045 , C07K1/047 , C07K1/062 , C07K7/06 , C07K17/06 , C07K17/14 , C12Q1/6809 , C12Q1/6816 , C12Q1/6837 , C12Q1/6874 , C40B40/06 , C40B40/10 , C40B60/14 , C40B80/00 , G01N15/1475 , G01N21/253 , G01N21/6428 , G01N21/6452 , G03F7/00 , G03F7/26 , G03F7/265 , G03F7/38 , G11C13/0014 , G11C13/0019 , H01L25/50 , H01L2924/0002 , Y02P20/55 , Y10S435/961 , Y10S435/968 , Y10S435/969 , Y10S435/973 , Y10S436/807 , Y10S436/809 , Y10T428/31971 , Y10T436/2575 , H01L2924/00
摘要： A method and device for preparing desired sequences on a substrate at known locations. Known locations (10) of a substrate (2) are irradiated by way of a mask (8) so as to activate a material (4) for binding. The substrate is then exposed to a first material (12) for binding thereto. Second locations (14) are then irradiated through a mask and exposed to a second material (16). A variety of sequences may be formed through selective irradiation of the substrate followed by application of selected materials. A reactor system and fluorescence detection system are also disclosed.

13. 发明申请

WO1996040987A1 PEPTIDE LIBRARY AND SCREENING METHOD 审中-公开
标题翻译：肽库和筛选方法
公开(公告)号：WO1996040987A1
公开(公告)日：1996-12-19
申请号：PCT/US1996009809
申请日：1996-06-07
申请人： AFFYMAX TECHNOLOGIES N.V. , SCHATZ, Peter, J. , CULL, Millard, G. , MILLER, Jeff, F. , STEMMER, Willem, P., C. , GATES, Christian, M.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C12Q01/66
CPC分类号： C12N15/1075 , C12Q1/6811
摘要： A random peptide library constructed by transforming host cells with a collection of recombinant vectors that encode a fusion protein comprised of a DNA binding protein and a random peptide and also encode a binding site for the DNA binding protein can be used to screen for novel ligands. The screening method results in the formation of a complex comprising the fusion protein bound to a receptor through the random peptide ligand and to the recombinant DNA vector through the DNA binding protein.
摘要翻译：通过用编码由DNA结合蛋白和随机肽组成的融合蛋白的重组载体的集合转化宿主细胞构建的随机肽文库，并且编码DNA结合蛋白的结合位点可用于筛选新配体。筛选方法导致通过DNA结合蛋白形成包含通过随机肽配体与受体结合的融合蛋白和重组DNA载体的复合物。

14. 发明申请

WO1995035278A1 METHODS FOR SYNTHESIZING DIVERSE COLLECTIONS OF PYRROLIDINE COMPOUNDS 审中-公开
标题翻译：合成吡咯啉化合物的多样性收集方法
公开(公告)号：WO1995035278A1
公开(公告)日：1995-12-28
申请号：PCT/US1995007878
申请日：1995-06-22
申请人： AFFYMAX TECHNOLOGIES N.V. , GALLOP, Mark, A. , MURPHY, Martin, A.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C07D207/12
CPC分类号： C07D207/16 , B01J2219/00592 , B01J2219/0072
摘要： Disclosed are methods for synthesizing very large collections of diverse pyrrolidine compounds and derivatives thereof on solid supports and synthetic compound libraries comprising pyrrolidine groups and derivatives thereof prepared by such methods.
摘要翻译：公开了用于在固体支持物上合成非常大量的各种吡咯烷化合物及其衍生物的方法，其包含通过这些方法制备的吡咯烷基团及其衍生物的合成化合物文库。

15. 发明申请

WO1995033846A1 BIOARRAY CHIP REACTION APPARATUS AND ITS MANUFACTURE 审中-公开
标题翻译：生物芯片反应装置及其制造
公开(公告)号：WO1995033846A1
公开(公告)日：1995-12-14
申请号：PCT/US1995007377
申请日：1995-06-08
申请人： AFFYMAX TECHNOLOGIES N.V. , BESEMER, Donald, M. , GOSS, Virginia, W. , WINKLER, James, L.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C12Q01/00
CPC分类号： B01L3/502715 , B01F13/0059 , B01F13/02 , B01F13/08 , B01J19/0046 , B01J2219/00371 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00531 , B01J2219/00536 , B01J2219/0054 , B01J2219/00547 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00659 , B01J2219/00662 , B01J2219/00689 , B01J2219/00695 , B01J2219/00711 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01L3/5027 , B01L3/502707 , B01L9/527 , B01L2200/025 , B01L2200/027 , B01L2200/0689 , B01L2200/12 , B01L2300/0636 , B01L2300/0816 , B01L2300/0825 , B01L2300/0877 , B01L2400/0487 , B82Y30/00 , C07H21/00 , C12Q1/6874 , C40B40/06 , C40B40/10 , C40B40/12 , C40B60/14 , C40B70/00 , G01N33/543 , G01N33/54366 , G01N2035/00158 , Y10S435/97 , Y10S435/973 , Y10S436/809 , Y10T436/112499
摘要： A body (2700) having a cavity (2710) for mounting a substrate (2790) fabricated with probe sequences (2795) at known locations according to the methods disclosed in United States Patent Number 5,153,854 and PCT WO 92/10092, for example, is provided. The cavity (2710) includes inlets (2750 and 2751) for introducing selected fluids into the cavity (2710) to contact the probes (2795). Accordingly, a commercially feasible device (2700) for use in high throughput assay systems is provided.
摘要翻译：例如，根据美国专利号5,153,854和PCT WO 92/10092中公开的方法，在已知位置处具有用于安装用探针序列（2795）制成的基底（2790）的腔体（2700）是提供。空腔（2710）包括用于将选定流体引入空腔（2710）中以接触探针（2795）的入口（2750和2751）。因此，提供了用于高通量测定系统的商业上可行的装置（2700）。

16. 发明申请

WO1995022058A1 METHOD AND APPARATUS FOR DETECTION OF FLUORESCENTLY LABELED MATERIALS 审中-公开
标题翻译：用于检测荧光标签材料的方法和装置
公开(公告)号：WO1995022058A1
公开(公告)日：1995-08-17
申请号：PCT/US1995001886
申请日：1995-02-09
申请人： AFFYMAX TECHNOLOGIES N.V. , STERN, David , FIEKOWSKY, Peter , TRULSON, Mark
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： G01N33/552
CPC分类号： G01N21/6458 , B01J2219/00605 , B01J2219/00612 , B01J2219/00659 , B01J2219/00702 , G01N21/6428 , G01N2021/174
摘要： Fluorescently marked targets bind to a substrate (230) synthesized with polymer sequences at known locations. The substrate (230) is mounted on an x-y-z translation table (240) during detection. The targets are detected by exposing selected regions of the substrate (230) to light from a light source (100) and detecting the light photons fluoresced therefrom, moving the substrate (230) position, and repeating the exposure and detection steps until the substrate (230) has been completely examined. The device includes a controller (190) for controlling the x-y-z translation table (240) movement for exposure of the selected regions of the substrate (230), the detection of the fluoresced light, and positioning of the substrate (230) to provide proper focusing of the light on the substrate (230) for maximum sensitivity. The resulting data can be used to determine binding affinity of the targets to the specific polymer sequences.
摘要翻译：荧光标记的靶结合到在已知位置处与聚合物序列合成的底物（230）。在检测期间，将衬底（230）安装在x-y-z平移台（240）上。通过将衬底（230）的选定区域暴露于来自光源（100）的光并检测从其发荧光的光子，移动衬底（230）位置并重复曝光和检测步骤直到衬底（ 230）已经完全检查。该装置包括控制器（190），用于控制用于曝光衬底（230）的选定区域的xyz平移台（240）运动，荧光灯的检测和衬底（230）的定位以提供适当的聚焦的基板（230）上的光以获得最大的灵敏度。所得数据可用于确定靶向特定聚合物序列的结合亲合力。

17. 发明申请

WO1995011995A1 ARRAYS OF NUCLEIC ACID PROBES ON BIOLOGICAL CHIPS 审中-公开
标题翻译：核酸探针在生物科学领域的阵列
公开(公告)号：WO1995011995A1
公开(公告)日：1995-05-04
申请号：PCT/US1994012305
申请日：1994-10-26
申请人： AFFYMAX TECHNOLOGIES N.V. , CHEE, Mark , CRONIN, Maureen, T. , FODOR, Stephen, P., A. , GINGERAS, Thomas, R. , HUANG, Xiaohua, C. , HUBBELL, Earl, A. , LIPSHUTZ, Robert, J. , LOBBAN, Peter, E. , MIYADA, Charles, Garrett , MORRIS, MacDonald, S. , SHAH, Nila , SHELDON, Edward, L.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C12Q01/68
CPC分类号： B82Y30/00 , B01J2219/00432 , B01J2219/00529 , B01J2219/00608 , B01J2219/00626 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , C07B2200/11 , C07H21/00 , C12Q1/6837 , C12Q1/6874 , C40B40/06 , C40B60/14 , C12Q2525/197 , C12Q2525/161
摘要： The invention provides chips of immobilized probes, and methods employing the chips, for comparing a reference polynucleotide sequence of known sequence with a target sequence showing substantial similarity with the reference sequence, but differing in the presence of e.g., mutations.
摘要翻译：本发明提供了固定化探针的芯片，以及使用该芯片的方法，用于将已知序列的参考多核苷酸序列与显示与参考序列基本相似的靶序列进行比较，但在例如突变的存在下不同。

18. 发明申请

WO1994017792A2 COMPOSITIONS AND METHODS FOR TRANSDERMAL DRUG DELIVERY 审中-公开
标题翻译：用于转管药物递送的组合物和方法
公开(公告)号：WO1994017792A2
公开(公告)日：1994-08-18
申请号：PCT/US1994000896
申请日：1994-01-26
申请人： AFFYMAX TECHNOLOGIES N.V. , SELICK, Harold, E. , OLDENBURG, Kevin, R.
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： A61K31/17
CPC分类号： A61K31/70 , A61K9/0009 , A61K9/1272 , A61K31/568 , A61K47/541 , A61K47/6957
摘要： The present invention relates to methods of delivering nucleotide-based pharmaceutical agents across membranes, such as the dermis or skin layer of a patient using iontophoresis.
摘要翻译：本发明涉及通过离子渗透法在患者体内递送核苷酸药物诸如真皮层或皮肤层的方法。

19. 发明申请

WO1997041093A1 METHODS FOR THE SYNTHESIS OF FMOC PROTECTED AMINES 审中-公开
标题翻译：合成FMOC保护胺的方法
公开(公告)号：WO1997041093A1
公开(公告)日：1997-11-06
申请号：PCT/NL1997000235
申请日：1997-04-29
申请人： AFFYMAX TECHNOLOGIES N.V. , BAER, Ted, Allen , RAILLARD, Stephen, Peter
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C07C269/04
CPC分类号： C07D207/16 , C07C269/04 , C07C319/20 , C07C2603/18 , C07C323/59
摘要： Methods for the synthesis of Fmoc-protected amines are provided. These methods involve the treatment of an amine component with a silylating agent and then an activated Fmoc-reagent. The methods can be conducted under anhydrous conditions.
摘要翻译：提供了合成Fmoc保护的胺的方法。这些方法涉及用甲硅烷基化剂处理胺组分，然后用活化的Fmoc-试剂处理。该方法可以在无水条件下进行。

20. 发明申请

WO1997010365A1 EXPRESSION MONITORING BY HYBRIDIZATION TO HIGH DENSITY OLIGONUCLEOTIDE ARRAYS 审中-公开
标题翻译：通过杂交到高密度寡糖核糖体阵列的表达监测
公开(公告)号：WO1997010365A1
公开(公告)日：1997-03-20
申请号：PCT/US1996014839
申请日：1996-09-13
申请人： AFFYMAX TECHNOLOGIES N.V. , LOCKHART, David, J. , BROWN, Eugene, L. , WONG, Gordon , CHEE, Mark , GINGERAS, Thomas, R. , MITTMANN, Michael, P. , LIPSHUTZ, Robert, J. , FODOR, Stephen, P., A. , WANG, Chunwei
发明人： AFFYMAX TECHNOLOGIES N.V.
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6809 , C12Q1/6837 , G01N15/1475 , C12Q2565/507
摘要： This invention provides methods of monitoring the expression levels of a multiplicity of genes. The methods involve hybridizing a nucleic acid sample to a high density array of oligonucleotide probes where the high density array contains oligonucleotide probes complementary to subsequences of target nucleic acids in the nucleic acid sample. In one embodiment, the method involves providing a pool of target nucleic acids comprising RNA transcripts of one or more target genes, or nucleic acids derived from the RNA transcripts, hybridizing said pool of nucleic acids to an array of oligonucleotide probes immobilized on surface, where the array comprising more than 100 different oligonucleotides and each different oligonucleotide is localized in a predetermined region of the surface, the density of the different oligonucleotides is greater than about 60 different oligonucleotides per 1 cm , and the oligonucleotide probes are complementary to the RNA transcripts or nucleic acids derived from the RNA transcripts; and quantifying the hybridized nucleic acids in the array.
摘要翻译：本发明提供监测多种基因的表达水平的方法。所述方法包括将核酸样品与寡核苷酸探针的高密度阵列杂交，其中高密度阵列含有与核酸样品中靶核酸的子序列互补的寡核苷酸探针。在一个实施方案中，所述方法包括提供包含一个或多个靶基因的RNA转录物或衍生自RNA转录物的核酸的靶核酸库，将所述核酸库与固定在表面上的寡核苷酸探针阵列杂交，其中包含超过100种不同寡核苷酸的阵列和每种不同的寡核苷酸被定位在表面的预定区域中，不同寡核苷酸的密度大于每1cm 2大约60个不同的寡核苷酸，寡核苷酸探针与衍生自RNA转录物的RNA转录物或核酸; 并定量阵列中的杂交核酸。

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