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81. 发明授权

US06563581B1 Scanning system and method for scanning a plurality of samples 有权
标题翻译：用于扫描多个样本的扫描系统和方法
公开(公告)号：US06563581B1
公开(公告)日：2003-05-13
申请号：US09617549
申请日：2000-07-14
申请人： Mark F. Oldham , Eugene F. Young
发明人： Mark F. Oldham , Eugene F. Young
IPC分类号： G01J330
CPC分类号： G01N21/6452 , B01L3/5085 , B01L2200/025 , B01L2300/0806 , B01L2300/0819
摘要： A system for detecting fluorescence emitted from a plurality of samples in a sample tray is provided. The system generally includes a plurality of lenses positioned in a linear arrangement, a linear actuator configured to translate the plurality of lenses, an excitation light source for generating an excitation light, an excitation light direction mechanism for directing the excitation light to a single lens of the plurality of lenses at a time so that a single sample holder aligned with the lens is illuminated at a time, and an optical detection system for analyzing light from the sample holders. In certain embodiments, the optical detection system includes a light dispersing element configured to spectrally disperse the light from the sample holder being illuminated, and a lens element configured to receive light from the light dispersing element and direct the light onto a light detection device. A method of scanning a sample tray having a plurality of samples positioned in sample holders to detect fluorescence is also provided.
摘要翻译：提供了一种用于检测从样品盘中的多个样品发出的荧光的系统。该系统通常包括以线性布置定位的多个透镜，被配置为平移多个透镜的线性致动器，用于产生激发光的激发光源，用于将激发光引导到单个透镜的激发光方向机构一次使多个透镜一次照射与镜片对准的单个样品保持器，以及用于分析来自样品保持器的光的光学检测系统。在某些实施例中，光学检测系统包括光分散元件，其被配置为光谱地分散来自被照亮的样品保持器的光;以及透镜元件，被配置为接收来自光分散元件的光并将光引导到光检测装置。还提供了扫描具有位于样品架中的多个样品以检测荧光的样品盘的方法。

82. 发明授权

US06506343B1 Multi-well microfiltration apparatus and method for avoiding cross-contamination 有权
标题翻译：多孔微滤装置及避免交叉污染的方法
公开(公告)号：US06506343B1
公开(公告)日：2003-01-14
申请号：US09565202
申请日：2000-05-04
申请人： Kevin S. Bodner , Alfred P. Madden , Jason H. Halsey , Mark F. Oldham , Stephen E. Moring , Jon Hoshizaki
发明人： Kevin S. Bodner , Alfred P. Madden , Jason H. Halsey , Mark F. Oldham , Stephen E. Moring , Jon Hoshizaki
IPC分类号： B01D3500
CPC分类号： B01L3/5025 , B01D61/18 , B01L3/50255 , B01L2400/049 , G01N35/0099 , G01N35/028 , G01N35/1074 , G01N2035/00485 , G01N2035/102 , Y10T436/25
摘要： The present invention provides multi-well plates and column arrays in which samples (e.g., cell lysates containing nucleic acids of interest, such as RNA) can be analyzed and/or processed. In one embodiment, the microfiltration arrangement is a multilayer structure, including (i) a column plate having an array of minicolumns into which samples can be placed, (ii) a discrete filter element disposed in each minicolumn, (iii) a drip-director plate having a corresponding array of drip directors through which filtrate may egress, and (iv) a receiving-well plate having a corresponding array of receiving wells into which filtrate can flow. The invention provides multi-well microfiltration arrangements that are relatively simple to manufacture and that overcome many of the problems associated with the prior arrangements relating to (i) cross-contamination due to wicking across a common filter sheet or (ii) individual filter elements entrapping sample constituents within substantial dead volumes. Further, the invention provides multi-well microfiltration arrangements that adequately support discrete filter elements disposed in the wells without creating substantial preferential flow. Additionally, the invention provides multi-well microfiltration arrangements that avoid cross-contamination due to aerosol formation, pendent drops and/or splattering. Other disclosed features of the invention provide for the automated covering or heat-sealing of filtrate samples separately collected in an array of wells.
摘要翻译：本发明提供了多孔板和柱阵列，其中可以分析和/或处理样品（例如，含有目标核酸的细胞裂解物，例如RNA）。在一个实施方案中，微滤装置是多层结构，包括（i）具有可以放置样品的小柱阵列的柱板，（ii）设置在每个小柱中的离散过滤元件，（iii）滴液导向器板具有相应的滴液导向器阵列，滤液可以通过其排出;以及（iv）接收孔板，其具有滤液能够流过的相应阵列的接收孔。本发明提供了相对简单的多孔微量过滤装置，并且克服了许多与先前布置有关的问题，这些问题涉及（i）由于芯毛穿过公共过滤片引起的交叉污染或（ii）截留的单个过滤器元件大量死体积的样品成分。此外，本发明提供了多孔微过滤装置，其充分地支撑设置在井中的分立的过滤元件，而不产生实质的优先流动。此外，本发明提供了多孔微滤装置，其避免了由于气溶胶形成，悬垂滴和/或飞溅而导致的交叉污染。本发明的其它公开的特征提供了分别收集在孔阵列中的滤液样品的自动覆盖或热封。

83. 发明授权

US09285297B2 Device, system, and method for depositing processed immiscible-fluid-discrete-volumes 有权
标题翻译：用于存放经处理的不混溶流体离散体的装置，系统和方法
公开(公告)号：US09285297B2
公开(公告)日：2016-03-15
申请号：US11507733
申请日：2006-08-22
申请人： Benjamin G. Schroeder , David M. Cox , Mark F. Oldham , Richard T. Reel , Willy Wiyatno
发明人： Benjamin G. Schroeder , David M. Cox , Mark F. Oldham , Richard T. Reel , Willy Wiyatno
IPC分类号： C12Q1/68 , C12P19/34 , G01N1/14 , B01L3/00 , F15C5/00 , F16K99/00 , G01N35/08 , B01L3/02 , B01L7/00
CPC分类号： C12Q1/6869 , B01J2219/00353 , B01J2219/0036 , B01J2219/00364 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00657 , B01J2219/00659 , B01L3/0293 , B01L3/502715 , B01L3/502784 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2300/0864 , B01L2300/0867 , B01L2400/0421 , B01L2400/0487 , C12Q1/6806 , C12Q1/6874 , F15C5/00 , F16K99/0001 , F16K99/0011 , F16K99/0013 , F16K2099/0084 , G01N1/14 , G01N27/44743 , G01N27/44769 , G01N35/08 , Y10T137/0318 , Y10T137/4259 , Y10T137/85978 , Y10T137/85986 , Y10T137/86863 , Y10T436/2575 , C12Q2535/101
摘要： Various embodiments relate to systems and/or methods for sample preparation that can be used for biochemical and/or molecular biology procedures involving small volumes, for example, micro volumes or smaller. Methods and systems that can reduce sample size requirements and increase the number of samples on a substrate are provided. Samples can be applied to a plate or other appropriate substrate and can be used for, inter alia, sequencing reactions. In some embodiments, apparatuses, systems, and/or methods for charged analyte collection are provided. Charged analytes in a sample can be electrokinetically collected or extracted from a conduit through a hole formed in a sidewall of the conduit, by application of an electric field that causes the charged analytes to migrate in a direction that is transverse to the conduit.
摘要翻译：各种实施方案涉及用于样品制备的系统和/或方法，其可用于涉及小体积例如微量或更小的生物化学和/或分子生物学过程。提供了可以减少样品尺寸要求并增加基材上样品数量的方法和系统。样品可以施用于板或其它合适的底物，并且可以用于尤其是测序反应。在一些实施例中，提供了用于带电分析物收集的装置，系统和/或方法。通过施加导致带电分析物沿横向于导管的方向迁移的电场，样品中的带电分析物可以通过形成在导管侧壁中的孔从导管电动收集或提取。

84. 发明申请

US20130034880A1 CHAMBER FREE NANOREACTOR SYSTEM 有权
标题翻译：无框架NANOREACTOR系统
公开(公告)号：US20130034880A1
公开(公告)日：2013-02-07
申请号：US13481858
申请日：2012-05-27
申请人： Mark F. Oldham
发明人： Mark F. Oldham
IPC分类号： C12P19/34 , C12M1/34
CPC分类号： C12Q1/6874 , B01L3/502738 , B01L3/502761 , B01L7/52 , B01L2200/0652 , B01L2200/0668 , B01L2200/0689 , B01L2200/10 , B01L2300/0645 , B01L2300/0816 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/123 , B01L2400/0415 , B01L2400/0418 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0478 , B01L2400/0655 , B03C1/286 , B03C1/288 , B03C1/30 , B03C5/005 , B03C5/022 , B03C5/026 , B03C2201/26 , C12Q1/6848 , C12Q1/6853 , C12Q1/6869 , C40B60/10 , G01N1/40 , G01N27/4145 , G01N27/4146 , G01N33/5438 , G01N2001/4038 , H01L29/00 , C12Q2523/307 , C12Q2563/143 , C12Q2565/543 , C12Q2563/116 , C12Q2565/607
摘要： Aspects of the invention include methods for improving the accuracy and read length of sequencing reactions by utilizing unlabeled unincorporable nucleotides, or by rephasing colony based sequencing reactions. Other aspects include systems and devices for improved measurement of biological reactions associated with bead which may be removed, utilizing current measurement methods through the counter ions associated with said beads due to the presence of reactants bound or associated with said bead, wherein electrodes for generating and measuring said current may be within the Debye length of said bead. Other aspects of the invention include methods for determining concentrations of input samples, means for reuse of an array, methods and apparatus for separating beads with different charge levels from each other.
摘要翻译：本发明的方面包括通过利用未标记的非可合并核苷酸或通过重新构建基于集落的测序反应来提高测序反应的准确性和读取长度的方法。其他方面包括用于改进与珠粒相关的生物反应测量的系统和装置，其可以通过与所述珠粒相关联的抗衡离子的电流测量方法，由于存在与所述珠粒结合或相关联的反应物，其中用于产生和测量所述电流可以在所述焊道的德拜长度内。本发明的其它方面包括用于确定输入样品的浓度的方法，用于重复使用阵列的装置，用于分离具有不同电荷水平的珠子的方法和装置。

85. 发明申请

US20130011848A1 Optical Instrument Including Excitation Source 有权
标题翻译：包括激发源的光学仪器
公开(公告)号：US20130011848A1
公开(公告)日：2013-01-10
申请号：US13611462
申请日：2012-09-12
申请人： Steven J. Boege , Howard G. King , Eugene F. Young , Johannes P. Sluis , Mark F. Oldham
发明人： Steven J. Boege , Howard G. King , Eugene F. Young , Johannes P. Sluis , Mark F. Oldham
IPC分类号： G01N21/75 , C12M1/40
CPC分类号： G01N21/6486 , B01L7/52 , C12Q2545/101 , G01N21/6452 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6441 , G01N2021/6463 , G01N2021/6482 , G01N2201/0245 , G01N2201/0628 , G01N2201/0635 , Y10S435/808 , Y10S435/809
摘要： An optical instrument is provided for simultaneously illuminating two or more spaced-apart reaction regions with excitation beams generated by a light source. The light source can include an area light array of light emitting diodes, one or more solid state lasers, one or more micro-wire lasers, or a combination thereof. According to various embodiments, a Fresnel lens can be disposed along a beam bath between the light source and the reaction regions. Methods of analysis using the optical instrument are also provided.
摘要翻译：提供了一种光学仪器，用于同时用由光源产生的激发光束照射两个或更多个间隔开的反应区域。光源可以包括发光二极管的区域光阵列，一个或多个固态激光器，一个或多个微线激光器，或其组合。根据各种实施例，菲涅耳透镜可以沿着光源和反应区域之间的光束槽设置。还提供了使用光学仪器的分析方法。

86. 发明申请

US20120087832A1 Apparatus and Method for Differentiating Multiple Fluorescence Signals by Excitation Wavelength 有权
标题翻译：通过激发波长区分多个荧光信号的装置和方法
公开(公告)号：US20120087832A1
公开(公告)日：2012-04-12
申请号：US13333788
申请日：2011-12-21
申请人： Howard G. King , Steven J. Boege , Eugene F. Young , Mark F. Oldham
发明人： Howard G. King , Steven J. Boege , Eugene F. Young , Mark F. Oldham
IPC分类号： G01N21/64 , G01N21/63
CPC分类号： G01N21/6428 , B01L7/52 , B01L2300/1805 , G01N21/6452 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441 , G01N2201/062 , G01N2201/0627 , G01N2201/0628 , Y10S435/808
摘要： An apparatus and method are provided for differentiating multiple detectable signals by excitation wavelength. The apparatus can include a light source that can emit respective excitation light wavelengths or wavelength ranges towards a sample in a sample retaining region, for example, in a well. The sample can contain two or more detectable markers, for example, fluorescent dyes, each of which can be capable of generating increased detectable emissions when excited in the presence of a target component. The detectable markers can have excitation wavelength ranges and/or emission wavelength ranges that overlap with the ranges of the other detectable markers. A detector can be arranged for detecting an emission wavelength or wavelength range emitted from a first marker within the overlapping wavelength range of at least one of the other markers.
摘要翻译：提供一种用于通过激发波长区分多个可检测信号的装置和方法。该装置可以包括可以例如在一个井中的样品保留区域中的样品发射相应的激发光波长或波长范围的光源。样品可以包含两种或更多种可检测的标记，例如荧光染料，其中当荧光染料在靶组分存在下被激发时，其能够产生增加的可检测的发射。可检测标记物可以具有与其它可检测标记物的范围重叠的激发波长范围和/或发射波长范围。可以设置检测器，用于检测在至少一个其他标记的重叠波长范围内从第一标记发射的发射波长或波长范围。

87. 发明授权

US07978326B2 DNA sequencing system 有权
标题翻译： DNA测序系统
公开(公告)号：US07978326B2
公开(公告)日：2011-07-12
申请号：US12630683
申请日：2009-12-03
申请人： Mark F. Oldham , Eric S. Nordman , Richard T. Reel , John S. Shigeura , Janice G. Shigeura, legal representative
发明人： Mark F. Oldham , Eric S. Nordman , Richard T. Reel , John S. Shigeura
IPC分类号： G01N21/00
CPC分类号： G01N27/44721 , B82Y10/00 , B82Y20/00 , G01N2015/149 , G01N2015/1497
摘要： An apparatus for detecting labeled beads is provided. The apparatus can include: one or more irradiation sources disposed for irradiating the one or more detection zones with radiation; at least one detector disposed for collecting charges corresponding to light signals emitted from labeled beads in the one or more detection zones, which have been excited by the radiation; and a system coupled to the at least one detector for effecting time delay integration of the charges by accumulating the charges before reading the charges at the output of the at least one detector.
摘要翻译：提供了一种用于检测标记珠粒的装置。该装置可以包括：一个或多个辐射源，被设置用于用辐射照射一个或多个检测区域; 至少一个检测器被设置用于收集对应于已经被辐射激发的一个或多个检测区域中的标记珠子发射的光信号的电荷; 以及耦合到所述至少一个检测器的系统，用于通过在读取至少一个检测器的输出端的电荷之前累积电荷来实现电荷的时间延迟积分。

88. 发明申请

US20110159549A1 Temperature control for light-emitting diode stabilization 审中-公开
标题翻译：温度控制用于发光二极管稳定
公开(公告)号：US20110159549A1
公开(公告)日：2011-06-30
申请号：US12895848
申请日：2010-09-30
申请人： Mark F. Oldham , Vinod L. Mirchandani
发明人： Mark F. Oldham , Vinod L. Mirchandani
IPC分类号： C12P19/34 , H01J7/24
CPC分类号： G01N21/64 , F21K9/00 , F21K9/64 , F21V29/54 , F21V29/677 , F21V29/74 , F21Y2115/10 , G01J3/0286 , G01J3/10 , G01N21/645 , G01N21/6452 , G01N2021/6439 , G01N2201/06113 , G01N2201/062 , G01N2201/0638 , G01N2201/1211 , G05D23/1919 , G05D23/20
摘要： A system is provided that includes a light-emitting diode (LED); a temperature sensor in thermal contact with the LED and capable of measuring an operating temperature and generating an operating temperature signal; and a temperature regulating system capable of receiving the operating temperature signal and regulating the operating temperature based on the operating temperature signal. A method for stabilizing the temperature of an LED is provided. A method is provided that includes providing a system comprising an LED, a reaction region, and a sample in the reaction region; generating excitation beams with the LED; directing excitation beams to the sample; detecting an optical property of the sample to obtain detection data; measuring the- operating temperature of the light emitting diode; and adjusting the detection data of an excitation beam characteristic shift related to the operating temperature, when the LED is operated at the operating temperature to generate the excitation beams.
摘要翻译：提供一种包括发光二极管（LED）的系统; 与LED热接触的温度传感器，能够测量工作温度并产生工作温度信号; 以及能够基于工作温度信号接收工作温度信号和调节工作温度的温度调节系统。提供了一种用于稳定LED的温度的方法。提供了一种方法，其包括在反应区域中提供包括LED，反应区域和样品的系统; 用LED产生激励光束; 将激发光束引导到样品; 检测样品的光学特性以获得检测数据; 测量发光二极管的工作温度; 并且当LED在工作温度下操作以产生激发光束时，调整与工作温度相关的激发光束特性偏移的检测数据。

89. 发明授权

US07955864B2 Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other 有权
标题翻译：使与第二流体接触的第一流体的离散体积彼此不混溶的装置和方法
公开(公告)号：US07955864B2
公开(公告)日：2011-06-07
申请号：US11508044
申请日：2006-08-22
申请人： David M. Cox , Willy Wiyatno , Mark F. Oldham , James C. Nurse , Douglas P. Greiner , Sam L. Woo , Richard T. Reel , Dennis A. Lehto , Linda G. Lee , Janusz B. Wojtowicz
发明人： David M. Cox , Willy Wiyatno , Mark F. Oldham , James C. Nurse , Douglas P. Greiner , Sam L. Woo , Richard T. Reel , Dennis A. Lehto , Linda G. Lee , Janusz B. Wojtowicz
IPC分类号： G01N1/00
CPC分类号： C12Q1/6869 , B01J2219/00353 , B01J2219/0036 , B01J2219/00364 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00657 , B01J2219/00659 , B01L3/0293 , B01L3/502715 , B01L3/502784 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2300/0864 , B01L2300/0867 , B01L2400/0421 , B01L2400/0487 , C12Q1/6806 , C12Q1/6874 , F15C5/00 , F16K99/0001 , F16K99/0011 , F16K99/0013 , F16K2099/0084 , G01N1/14 , G01N27/44743 , G01N27/44769 , G01N35/08 , Y10T137/0318 , Y10T137/4259 , Y10T137/85978 , Y10T137/85986 , Y10T137/86863 , Y10T436/2575 , C12Q2535/101
摘要： Various embodiments described in the application relate to an apparatus, system, and method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise an apparatus comprising at least one conduit operatively connected to one or more pumps for providing discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.
摘要翻译：在本申请中描述的各种实施例涉及用于在管道内产生与第二流体不混溶的一种或多种流体的离散体积的装置，系统和方法。离散体积可用于涉及小体积的生物化学或分子生物学过程，例如微升体积，纳升级体积或更小体积。该系统可以包括一种装置，该装置包括至少一个导管，其操作地连接到一个或多个泵，用于提供通过与离散体积的流体不混溶的流体彼此分离的离散体积，例如水不混溶流体 - 油分离体积。

90. 发明申请

US20110085168A1 Luminescence Reference Standards 有权
标题翻译：发光参考标准
公开(公告)号：US20110085168A1
公开(公告)日：2011-04-14
申请号：US12813389
申请日：2010-06-10
申请人： J. Michael Phillips , Aldrich N.K. Lau , Mark F. Oldham , Kevin S. Bodner , Steven J. Boege , Donald R. Sandell , David H. Tracy
发明人： J. Michael Phillips , Aldrich N.K. Lau , Mark F. Oldham , Kevin S. Bodner , Steven J. Boege , Donald R. Sandell , David H. Tracy
IPC分类号： G01J1/10 , G01B11/00 , B82Y99/00
CPC分类号： G01N21/278 , B82Y15/00 , G01J1/10 , G01N21/01 , G01N21/4785 , G01N21/6452 , G01N21/76 , G01N2201/06113 , G01N2201/0612 , G01N2201/062 , G01N2201/0691 , G01N2201/0697 , Y10S977/943 , Y10S977/949
摘要： The present teachings provide for systems, and components thereof, for detecting and/or analyzing light. These systems can include, among others, optical reference standards utilizing luminophores, such as nanocrystals, for calibrating, validating, and/or monitoring light-detection systems, before, during, and/or after sample analysis.
摘要翻译：本教导提供了用于检测和/或分析光的系统及其组件。这些系统尤其可以包括在样品分析之前，期间和/或之后利用发光体（例如纳米晶体）用于校准，验证和/或监测光检测系统的光学参考标准。

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