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1. 发明授权

US09152150B1 Compositions, systems, and methods for immiscible fluid discrete volume manipulation 有权
标题翻译：用于不混溶流体离散体积操纵的组合物，系统和方法
公开(公告)号：US09152150B1
公开(公告)日：2015-10-06
申请号：US12036037
申请日：2008-02-22
申请人： Linda G. Lee , Mark F. Oldham , Sam L. Woo , David M. Cox , Richard T. Reel , Peter N. Ma , Ben F. Johnson , Dennis Letho
发明人： Linda G. Lee , Mark F. Oldham , Sam L. Woo , David M. Cox , Richard T. Reel , Peter N. Ma , Ben F. Johnson , Dennis Letho
IPC分类号： G05D11/00 , G05D7/06 , B01L3/00 , B01F13/00 , G01N35/08 , B01L3/02
CPC分类号： B01F3/0811 , B01F3/0815 , B01F5/0471 , B01F13/0071 , B01F13/0072 , B01F13/0076 , B01F2003/0842 , B01L3/0265 , B01L3/502769 , B01L3/502784 , B01L2200/06 , B01L2200/0605 , B01L2200/0673 , B01L2300/0816 , B01L2300/0867 , B01L2400/0481 , B01L2400/0666 , G01N1/38 , G01N21/6428 , G01N35/08 , G01N2001/386 , G01N2021/6439 , G05D7/0694 , G05D11/131
摘要： Systems and methods of manipulating discrete volumes of a first fluid in a second fluid are provided. In some embodiments, discrete volumes can be formed in a conduit. In other embodiments, addition fluid can be added to a discrete volume in a first conduit by injecting the addition fluid at a relatively higher pressure. In some embodiments, discrete volumes that normally would not coalesce can be manipulated to be merged together.
摘要翻译：提供了在第二流体中操纵第一流体的离散体积的系统和方法。在一些实施例中，离散体积可以形成在导管中。在其它实施方案中，可以通过在相对较高的压力下注入添加流体将添加流体添加到第一导管中的离散体积。在一些实施例中，通常不会聚结的离散体积可以被操纵以被合并在一起。

2. 发明申请

US20120196295A1 UV Excitable Fluorescent Energy Transfer Dyes 有权
标题翻译：紫外线可激发荧光能量转移染料
公开(公告)号：US20120196295A1
公开(公告)日：2012-08-02
申请号：US13361863
申请日：2012-01-30
申请人： Linda G. Lee
发明人： Linda G. Lee
IPC分类号： G01N21/76 , C07D311/82 , C08B37/00 , C07K14/00 , C07K2/00 , C07D407/12 , C07D491/22
CPC分类号： C12Q1/6869 , C07H19/10 , C07H19/20 , C07H21/00 , C07H21/04 , C09B11/24 , C12Q1/6818 , C12Q2563/107
摘要： Novel energy transfer dyes which can be used with shorter wavelength light sources are provided. These dyes include a donor dye with an absorption maxima at a wavelength between about 250 to 450 nm and an acceptor dye which is capable of absorbing energy emitted from the donor dye. One of the energy transfer dyes has a donor dye which is a member of a class of dyes having a coumarin or pyrene ring structure and an acceptor dye which is capable of absorbing energy emitted from the donor dye, wherein the donor dye has an absorption maxima between about 250 and 450 nm and the acceptor dye has an emission maxima at a wavelength greater than about 500 nm.
摘要翻译：提供了可用于较短波长光源的新型能量转移染料。这些染料包括在约250至450nm之间的波长处具有最大吸收的供体染料和能够吸收从供体染料发射的能量的受体染料。能量转移染料之一具有供体染料，其是具有香豆素或芘环结构的一类染料的成员和能够吸收从供体染料发射的能量的受体染料，其中供体染料具有吸收最大值在约250和450nm之间，受体染料具有波长大于约500nm的发射最大值。

3. 发明申请

US20090215062A1 COMPOSITION AND METHOD FOR SEQUENCING NUCLEIC ACID 有权
标题翻译：用于测序核酸的组合物和方法
公开(公告)号：US20090215062A1
公开(公告)日：2009-08-27
申请号：US12365140
申请日：2009-02-03
申请人： LINDA G. LEE
发明人： LINDA G. LEE
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6848 , C12Q1/6853 , C12Q2521/325 , C12Q2525/125 , C12Q2535/101
摘要： A composition for sequencing DNA is provided and comprises a nuclease and a nuclease-resistant sequencing primer. A method of preparing DNA for sequencing and a method of sequencing DNA are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the nuclease-resistant sequencing primer is essentially non-degraded.
摘要翻译：提供了用于测序DNA的组合物，并且包含核酸酶和核酸酶抗性测序引物。还提供了制备用于测序的DNA的方法和测序DNA的方法。测序DNA的方法可以包括在扩增引物被核酸酶降解的条件下将扩增反应产物与组合物接触，并且核酸酶抗性测序引物基本上是未降解的。

4. 发明授权

US07550570B2 4,7-dichlororhodamine dyes labeled polynucleotides 失效
标题翻译： 4,7-二氯罗丹明染料标记的多核苷酸
公开(公告)号：US07550570B2
公开(公告)日：2009-06-23
申请号：US10788660
申请日：2004-02-26
申请人： Linda G. Lee , Scott C. Benson , Barnett B. Rosenblum , Sandra L. Spurgeon , Ronald J. Graham
发明人： Linda G. Lee , Scott C. Benson , Barnett B. Rosenblum , Sandra L. Spurgeon , Ronald J. Graham
IPC分类号： C07G3/00 , C07H19/04 , C12Q1/68
CPC分类号： C07D401/06 , C07D401/14 , C07D405/14 , C07D491/147 , C12Q1/6869 , C12Q2563/107 , C12Q2535/101
摘要： A set of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structures wherein R1-R6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or, when taken together, R1 and R6 is benzo, or, when taken together, R4 and R5 is benzo; R7-R10, R12-R16 and R18 may be hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group; R11 and R17 may be hydrogen, lower alkyl, lower alkene, lower alkyne, phenyl, aryl, linking group; Y1-Y4 are hydrogen, lower alkyl, or cycloalkyl, or, when taken together, Y1 and R2, Y2 and R1 Y3 and R3, and/or Y4 and R4 is propano, ethano, or substituted forms thereof; and X1-X3 taken separately are hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonate, hydroxymethyl, and linking group, or any combinations thereof. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.
摘要翻译：公开了一组用作荧光染料的4,7-二氯罗丹明化合物，其中R1-R6是氢，氟，氯，低级烷基，低级烯，低级炔，磺酸，砜，氨基，酰氨基，腈，低级烷氧基或连接基团时，或者当R 1和R 6一起时为苯并，或者合并时，R 4和R 5为苯并; R7-R10，R12-R16和R18可以是氢，氟，氯，低级烷基，低级烯烃，低级炔烃，磺酸盐，砜，氨基，酰氨基，腈，低级烷氧基，连接基团; R11和R17可以是氢，低级烷基，低级烯，低级炔，苯基，芳基，连接基团; Y1-Y4是氢，低级烷基或环烷基，或者当一起为Y1和R2，Y2和R1Y3和R3，和/或Y4和R4为丙酰，乙酸或其取代形式时; 单独使用的X 1 -X 3是氢，氯，氟，低级烷基，羧酸酯，磺酸酯，羟甲基和连接基团，或其任何组合。另一方面，本发明包括用4,7-二氯罗丹明染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸和多核苷酸。在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。

5. 发明申请

US20090093623A1 4,7-DICHLORORHODAMINE DYES 审中-公开
标题翻译： 4,7-二氯丹染色体
公开(公告)号：US20090093623A1
公开(公告)日：2009-04-09
申请号：US12199441
申请日：2008-08-27
申请人： Linda G. Lee , Scott C. Benson , Barnett B. Rosenblum , Sandra L. Spurgeon , Ronald J. Graham
发明人： Linda G. Lee , Scott C. Benson , Barnett B. Rosenblum , Sandra L. Spurgeon , Ronald J. Graham
IPC分类号： C07H21/00 , C07H19/10
CPC分类号： C07D401/06 , C07D401/14 , C07D405/14 , C07D491/147 , C12Q1/6869 , C12Q2563/107 , C12Q2535/101
摘要： A set of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structures wherein R1-R6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or, when taken together, R1 and R6 is benzo, or, when taken together, R4 and R5 is benzo; R7-R10, R12-R16 and R18 may be hydrogen, fluorine, chlorine, lower alkyl lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group; R11 and R17 may be hydrogen, lower alkyl lower alkene, lower alkyne, phenyl, aryl, linking group; Y1-Y4 are hydrogen, lower alkyl or cycloalkyl, or, when taken together, Y1 and R2, Y2 and R1 Y3 and R3, and/or Y4 and R4 is propano, ethano, or substituted forms thereof, and X1-X3 taken separately are hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonate, hydroxymethyl, and linking group, or any combinations thereof. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.
摘要翻译：公开了一组用作荧光染料的4,7-二氯罗丹明化合物，其中R1-R6是氢，氟，氯，低级烷基，低级烯，低级炔，磺酸，砜，氨基，酰氨基，腈，低级烷氧基或连接基团时，或者当R 1和R 6一起时为苯并，或者合并时，R 4和R 5为苯并; R7-R10，R12-R16和R18可以是氢，氟，氯，低级烷基低级烯，低级炔，磺酸，砜，氨基，酰胺基，腈，低级烷氧基，连接基团; R11和R17可以是氢，低级烷基低级烯，低级炔，苯基，芳基，连接基; Y1-Y4是氢，低级烷基或环烷基，或者当Y1和R2，Y2和R1Y3和R3和/或Y4和R4分别为丙酰，乙酸或其取代形式时，分别取X1-X3 是氢，氯，氟，低级烷基，羧酸酯，磺酸酯，羟甲基和连接基团，或其任何组合。另一方面，本发明包括用4,7-二氯罗丹明染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸和多核苷酸。在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。

6. 发明授权

US07452672B2 Methods of analyzing polynucleotides employing energy transfer dyes 失效
标题翻译：使用能量转移染料分析多核苷酸的方法
公开(公告)号：US07452672B2
公开(公告)日：2008-11-18
申请号：US11618683
申请日：2006-12-29
申请人： Linda G. Lee , Sandra L. Spurgeon , Barnett Rosenblum
发明人： Linda G. Lee , Sandra L. Spurgeon , Barnett Rosenblum
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/00 , C07H21/02
CPC分类号： C07H21/04 , C07H21/00 , C09B11/24 , C12Q1/6818
摘要： Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers facilitate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z1C(O)R22R28 where R21 is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z1 is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.
摘要翻译：提供了用于将供体染料与能量转移荧光染料中的受体染料连接的新型接头。这些接头促进能量转移染料中供体和受体染料之间的能量的有效转移。用于将供体染料与能量转移荧光染料中的受体染料连接的这些接头之一具有通式结构R 21 R 1 C（O）R 22 其中R 21是与供体染料连接的C 1-5烷基，C（O）是羰基（C 1 -C 4）烷基，基团Z 1是NH，硫或氧，R 22是包括烯烃，二烯烃，炔烃，具有至少一个碳原子的五元和六元环的取代基不饱和键或与羰基碳连接的稠合环结构，R 28包括将接头连接于受体染料的官能团。

7. 发明授权

US07423140B2 Oligonucleotides and analogs labeled with energy transfer dyes 有权
标题翻译：用能量转移染料标记的寡核苷酸和类似物
公开(公告)号：US07423140B2
公开(公告)日：2008-09-09
申请号：US11617667
申请日：2006-12-28
申请人： Linda G. Lee , Sandra L. Spurgeon , Barnett Rosenblum
发明人： Linda G. Lee , Sandra L. Spurgeon , Barnett Rosenblum
IPC分类号： C07H21/04 , C07H19/04 , C12Q1/68
CPC分类号： C07H21/04 , C07D311/82 , C07D311/90 , C07D405/12 , C07D405/14 , C07H21/00 , C09B11/04 , C09B11/24 , C09B23/083 , C09B69/00 , C12Q1/6818 , C12Q1/6869 , C12Q2563/107 , C12Q2535/101 , C12Q2565/1015
摘要： Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers facilitate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z1C(O)R22R28 where R21 is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z1 is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.
摘要翻译：提供了用于将供体染料与能量转移荧光染料中的受体染料连接的新型接头。这些接头促进能量转移染料中供体和受体染料之间的能量的有效转移。用于将供体染料与能量转移荧光染料中的受体染料连接的这些接头之一具有通式结构R 21 R 1 C（O）R 22 其中R 21是与供体染料连接的C 1-5烷基，C（O）是羰基（C 1 -C 4）烷基，基团Z 1是NH，硫或氧，R 22是包括烯烃，二烯烃，炔烃，具有至少一个碳原子的五元和六元环的取代基不饱和键或与羰基碳连接的稠合环结构，R 28包括将接头连接于受体染料的官能团。

8. 发明授权

US07166715B2 Nitro-substituted non-fluorescent asymmetric cyanine dye compounds 有权
标题翻译：硝基取代的非荧光不对称花青染料化合物
公开(公告)号：US07166715B2
公开(公告)日：2007-01-23
申请号：US10840189
申请日：2004-05-05
申请人： Linda G. Lee , Ronald J. Graham , Khairuzzaman B. Mullah , Francis T. Haxo
发明人： Linda G. Lee , Ronald J. Graham , Khairuzzaman B. Mullah , Francis T. Haxo
IPC分类号： C07H21/02 , C07H19/04 , C07D293/00 , C07D421/00 , C07D293/10 , C12Q1/68
CPC分类号： C07H21/00 , C09B23/02
摘要： The invention provides an asymmetric cyanine dye compound having the structure including substituted forms thereof, wherein, at least one of R1 and R2 is linking group, X is O, S, or Se, and n ranges from 0 to 2. The invention further provides reporter-quencher dye pairs comprising the asymmetric cyanine dyes, dye-labelled polynucleotides incorporating the asymmetric cyanine dyes, and hybridization detection methods utilizing the dye-labelled polynucleotides.
摘要翻译：本发明提供了具有包括其取代形式的结构的不对称花青染料化合物，其中R 1和R 2中的至少一个是连接基团，X是O，S 或Se，n为0至2.本发明还提供包含不对称花青染料，掺入不对称花青染料的染料标记的多核苷酸和利用染料标记的多核苷酸的杂交检测方法的报道猝灭剂染料对。

9. 发明授权

US07012141B2 UV excitable fluorescent energy transfer dyes 有权
标题翻译：紫外线激发荧光能量转移染料
公开(公告)号：US07012141B2
公开(公告)日：2006-03-14
申请号：US10359931
申请日：2003-02-07
申请人： Linda G. Lee
发明人： Linda G. Lee
IPC分类号： C07D213/00 , C07D215/00 , C07D221/22 , C07D263/62
CPC分类号： C12Q1/6869 , C07H19/10 , C07H19/20 , C07H21/00 , C07H21/04 , C09B11/24 , C12Q1/6818 , C12Q2563/107
摘要： Novel energy transfer dyes which can be used with shorter wavelength light sources are provided. These dyes include a donor dye with an absorption maxima at a wavelength between about 250 to 450 nm and an acceptor dye which is capable of absorbing energy emitted from the donor dye. One of the energy transfer dyes has a donor dye which is a member of a class of dyes having a coumarin or pyrene ring structure and an acceptor dye which is capable of absorbing energy emitted from the donor dye, wherein the donor dye has an absorption maxima between about 250 and 450 nm and the acceptor dye has an emission maxima at a wave length greater than about 500 nm.
摘要翻译：提供了可用于较短波长光源的新型能量转移染料。这些染料包括在约250至450nm之间的波长处具有最大吸收的供体染料和能够吸收从供体染料发射的能量的受体染料。能量转移染料之一具有供体染料，其是具有香豆素或芘环结构的一类染料的成员和能够吸收从供体染料发射的能量的受体染料，其中供体染料具有吸收最大值在约250和450nm之间，受体染料具有波长大于约500nm的发射最大值。

10. 发明授权

US06218124B1 Method for detecting oligonucleotides using UV light source 有权
标题翻译：使用UV光源检测寡核苷酸的方法
公开(公告)号：US06218124B1
公开(公告)日：2001-04-17
申请号：US09385230
申请日：1999-08-27
申请人： Linda G. Lee
发明人： Linda G. Lee
IPC分类号： C12Q168
CPC分类号： C07H21/00 , C12Q1/6818 , C12Q1/6869 , G01N21/33 , C12Q2565/1015
摘要： A method for detecting oligonucleotides is provided and comprises forming a series of different sized oligonucleotides labeled with an energy transfer dye; separating the series of labeled oligonucleotides based on size; and detecting the separated labeled oligonucleotide by exposing the oligonucleotides to light having a wavelength between about 250 and 450 nm and measuring light emitted by the energy transfer dye at a wavelength greater than about 500 nm.
摘要翻译：提供了一种用于检测寡核苷酸的方法，包括形成用能量转移染料标记的一系列不同大小的寡核苷酸; 基于大小分离一系列标记的寡核苷酸; 并通过将寡核苷酸暴露于波长在约250和450nm之间的光并测量能量转移染料在大于约500nm的波长下发射的光来检测分离的标记的寡核苷酸。

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