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    • 3. 发明申请
    • DEVICES AND METHODS FOR OPTOELECTRONIC MANIPULATION OF SMALL PARTICLES
    • 用于光电子操作小颗粒的装置和方法
    • US20100206731A1
    • 2010-08-19
    • US12702137
    • 2010-02-08
    • Aldrich N.K. LauJoon Mo YangHuan L. PhanSteven W. SherwoodHans A. Fuernkranz
    • Aldrich N.K. LauJoon Mo YangHuan L. PhanSteven W. SherwoodHans A. Fuernkranz
    • B03C11/00
    • B03C5/026B03C5/005B03C5/028C07K1/26G01N27/305
    • A method for sorting cells in a biological sample comprising a first type of cells and a second type of cells may comprise introducing the biological sample into a chamber comprising a first surface and a second surface, wherein the first surface is associated with a transparent electrode and the second surface is associated with a photoconductive portion of an electrode. The method may further comprise moving incident light and the photoconductive portion relative to one another so as to illuminate regions of the photoconductive portion and modulate an electric field in the chamber in proximity to the illuminated regions. The method may further comprise separating the first type of cells from the second type of cells in the chamber via dielectrophoretic movement of the first type of cells and the second type of cells caused by the modulated electric field, wherein a dielectrophoretic characteristic of at least one of the first type of cells and the second type of cells has been modified.
    • 用于分选生物样品中包含第一类型细胞和第二类型细胞的细胞的方法可以包括将生物样品引入包含第一表面和第二表面的室中,其中第一表面与透明电极相关联, 第二表面与电极的光电导部分相关联。 该方法还可以包括相对于彼此移动入射光和光电导部分,以照射光电导部分的区域并且调制在邻近照明区域的室中的电场。 该方法还可以包括通过由调制电场引起的第一类型的细胞和第二类型的细胞的介电电泳运动,将第一类型的细胞与腔室中的第二类型的细胞分离,其中至少一种的介电电泳特性 的第一类型的细胞和第二类型的细胞已被修改。
    • 5. 发明申请
    • APPARATUS AND METHODS FOR PREPARATION OF SUBTANTIALLY UNIFORM EMULSIONS CONTAINING A PARTICLE
    • 用于制备含有颗粒的亚基均匀乳液的装置和方法
    • US20090023189A1
    • 2009-01-22
    • US12119475
    • 2008-05-12
    • Aldrich N.K. LauJonathan M. CasselJohn A. Bridgham
    • Aldrich N.K. LauJonathan M. CasselJohn A. Bridgham
    • C12P19/34C12M1/40
    • B01F3/0807B01F5/0475B01F5/0476B01F5/0485B01F13/0059
    • Methods and systems for forming water-in-oil emulsions are described. For example, an apparatus is described which includes: a first compartment containing a plurality of particles dispersed in an aqueous phase; a second compartment containing an oil phase; a porous layer separating the first and second compartments; and a device for applying pressure to the first compartment. A method is described which includes: moving an oil phase relative to a surface of a porous layer while simultaneously forcing an aqueous composition comprising particles through the porous layer and into the flowing dispersion medium thereby forming droplets of the aqueous composition containing particles dispersed in the oil phase. The aqueous composition can include one or more nucleic acid templates and reagents for amplifying the nucleic acids such as PCR reagents. A porous partition is described comprising a first and second major surfaces and at least two straight through pores comprising a cross sectional shape selected from a polygon, an oval, an oblong, a dumbbell, a bowtie and irregular shapes thereof. Aqueous droplets containing an oligonucleotide attached to a particle and reagents can be used as a microreactor for nucleic acid amplification.
    • 描述了用于形成油包水乳液的方法和系统。 例如,描述了一种装置,其包括:包含分散在水相中的多个颗粒的第一隔室; 含有油相的第二隔室; 分离第一和第二隔室的多孔层; 以及用于向第一隔室施加压力的装置。 描述了一种方法,其包括:相对于多孔层的表面移动油相,同时迫使包含颗粒的水性组合物通过多孔层并进入流动分散介质中,由此形成含有分散在油中的颗粒的含水组合物的液滴 相。 水性组合物可以包括用于扩增核酸如PCR试剂的一种或多种核酸模板和试剂。 描述了一种多孔分隔件,其包括第一和第二主表面和至少两个直通孔,其包括选自多边形,椭圆形,长方形,哑铃,弓形和不规则形状的横截面形状。 含有与粒子连接的寡核苷酸的水滴和试剂可用作核酸扩增的微反应器。