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1. 发明申请

US20190249231A1 METHOD 审中-公开
公开(公告)号：US20190249231A1
公开(公告)日：2019-08-15
申请号：US16343580
申请日：2017-10-20
申请人： Oxford Nanopore Technologies Limited
发明人： Nicholas Antony Smith , Daniel John Turner , Daniel George Fordham , James White
IPC分类号： C12Q1/6825 , C12Q1/6876
CPC分类号： C12Q1/6825 , C12Q1/68 , C12Q1/6816 , C12Q2523/307 , C12Q2525/101 , C12Q2525/161 , C12Q2537/143 , C12Q2563/116 , C12Q2565/607 , C12Q2565/631
摘要： A method for determining the presence, absence or amount of two or more target polynucleotides in a sample comprising additional components, the method comprising: (i) contacting the sample with a panel of two or more probes under conditions suitable for hybridisation of the target polynucleotides to the probes, wherein: (a) each probe comprises a non-hybridisation region and a hybridisation region that specifically hybridises to one of the target polynucleotides to form a hybridised probe; and (b) the hybridisation region of a probe of the panel comprises one or more non-natural nucleotides; (ii) contacting the sample prepared in step (i) with a transmembrane pore through which a single stranded polynucleotide but not a double stranded polynucleotide can pass and applying a potential difference to the transmembrane pore such that the hybridised probes in the sample interact with the pore; (iii) measuring current blockades having a duration within a defined window, wherein: (a) the one or more non-natural nucleotides present in the hybridisation region of the probe increase or decrease the duration of the current blockade due to the probe hybridised to its target polynucleotide such that the proportion of current blockades that occur within the window due to the interaction of the hybridised probes with the pore is increased compared to when the corresponding one or more natural nucleotides are present in the hybridisation region; and (b) each hybridised probe gives rise to a current blockade indicative of that probe; and (iv) correlating the measured current blockades with the probes, thereby determining the presence, absence or amount of the two or more target polynucleotides in the sample.

2. 发明申请

US20190233464A1 Detection Comprising Signal Amplifier 审中-公开
公开(公告)号：US20190233464A1
公开(公告)日：2019-08-01
申请号：US16376498
申请日：2019-04-05
申请人： Helios Bioelectronics Inc.
发明人： Hardy Wai-Hong CHAN , Yuh-Shyong YANG , Wen-Yih CHEN
IPC分类号： C07H21/04 , C12Q1/6832 , C12Q1/6825 , C12Q1/682 , C12Q1/6804 , C12Q1/6837 , G01N33/543 , C12Q1/6841 , G01N33/53 , G01N21/552 , G01N33/552 , G01N27/414
CPC分类号： C07H21/04 , C12Q1/6804 , C12Q1/682 , C12Q1/6825 , C12Q1/6832 , C12Q1/6837 , C12Q1/6841 , G01N21/554 , G01N27/4145 , G01N33/5308 , G01N33/54373 , G01N33/54393 , G01N33/552 , C12Q2565/607 , C12Q2565/628 , C12Q2525/113
摘要： The present invention relates to a method for detecting a target molecule, comprising forming a capturing complex comprising the target molecule; and binding the capturing complex with a signal amplifier, wherein the capturing complex has a net electrical charge; the signal amplifier has affinity to the capturing complex and has a like net electrical charge of the net electrical charge of the capturing complex. The invention improves the detection sensitivity and sensing limit of the detection.

3. 发明申请

US20190041354A1 FIELD EFFECT SENSORS 审中-公开
公开(公告)号：US20190041354A1
公开(公告)日：2019-02-07
申请号：US16024299
申请日：2018-06-29
申请人： ILLUMINA, INC.
发明人： Boyan Boyanov
IPC分类号： G01N27/414 , H03K3/03 , C12Q1/6869 , H01L21/02
CPC分类号： G01N27/4145 , B82Y10/00 , B82Y15/00 , C12Q1/6869 , G01N27/4146 , G01R19/0092 , H01L21/0259 , H01L29/068 , H01L29/775 , H03K3/0315 , C12Q2563/157 , C12Q2565/607
摘要： Apparatus and methods are disclosed for single molecule field effect sensors having conductive channels functionalized with a single active moiety. A region of a nanostructure (e.g., such as a silicon nanowire or a carbon nanotube) provide the conductive channel. Trapped state density of the nanostructure is modified for a portion of the nanostructure in proximity with a location where the active moiety is linked to the nanostructure. In one example, the semiconductor device includes a source, a drain, a channel including a nanostructure having a modified portion with an increased trap state density, the modified portion being further functionalized with an active moiety. A gate terminal is in electrical communication with the nanostructure. As a varying electrical signal is applied to an ionic solution in contact with the nanostructure channel, changes in current observed from the semiconductor device can be used to identify composition of the analyte.

4. 发明申请

US20180328850A1 INTEGRATED DEVICE WITH EXTERNAL LIGHT SOURCE FOR PROBING DETECTING AND ANALYZING MOLECULES 审中-公开
公开(公告)号：US20180328850A1
公开(公告)日：2018-11-15
申请号：US16028159
申请日：2018-07-05
申请人： Quantum-Si Incorporated
发明人： Jonathan M. Rothberg , Ali Kabiri , Jason W. Sickler , Brett J. Gyarfas , Jeremy Lackey , Gerard Schmid , Benjamin Cipriany , Jack Jewell , Lawrence C. West , Michael Ferrigno , Paul E. Glenn , Anthony Bellofiore
IPC分类号： G01N21/64 , B01L3/00 , C12Q1/6869 , C12Q1/6874 , G01N21/77
CPC分类号： G01N21/6486 , B01L3/5085 , B01L2200/12 , B01L2300/0829 , B01L2300/0887 , B01L2300/0893 , B01L2300/168 , C12Q1/6869 , C12Q1/6874 , C12Q2521/101 , C12Q2537/157 , C12Q2563/107 , C12Q2565/607 , G01N21/64 , G01N21/645 , G01N21/6452 , G01N21/648 , G01N21/7743 , G01N21/7746 , G01N2021/6419 , G01N2021/6441 , G01N2021/6478 , G01N2201/02 , G01N2201/06113 , G01N2201/0612 , G01N2201/062 , G01N2201/068 , G01N2201/125 , Y10T29/49016 , C12Q2525/101
摘要： Apparatus and methods for analyzing single molecule and performing nucleic acid sequencing. An integrated device includes multiple pixels with sample wells configured to receive a sample, which, when excited, emits radiation; at least one element for directing the emission radiation in a particular direction; and a light path along which the emission radiation travels from the sample well toward a sensor. The apparatus also includes an instrument that interfaces with the integrated device. Each sensor may detect emission radiation from a sample in a respective sample well. The instrument includes an excitation light source for exciting the sample in each sample well.

5. 发明申请

US20180313777A1 INTEGRATED REFERENCE ELECTRODE AND FLUID DISPENSER 审中-公开
公开(公告)号：US20180313777A1
公开(公告)日：2018-11-01
申请号：US15725776
申请日：2017-10-05
申请人： Taiwan Semiconductor Manufacturing Co., Ltd.
发明人： Chin-Hua WEN , Jui-Cheng HUANG
IPC分类号： G01N27/30 , G01N27/414 , B01L3/00 , C12Q1/68 , G01N33/543
CPC分类号： G01N27/301 , B01L3/0262 , B01L3/502715 , B01L2200/06 , B01L2200/141 , B01L2300/0645 , C12Q1/6825 , G01N27/414 , G01N27/4145 , G01N33/5438 , C12Q2563/159 , C12Q2565/607 , C12Q2565/629
摘要： An apparatus including an integrated reference electrode and a fluid dispenser is described. The reference electrode includes a body and a tip. The fluid dispenser at least partially surrounds the tip of the reference electrode and includes an inlet, a chamber, and an outlet. The fluid dispenser is configured to receive a fluid sample from the inlet to the chamber and form a droplet of the fluid sample through the outlet so that the droplet is in fluidic contact with the tip of the reference electrode and associated with a known potential determined by the reference electrode.

6. 发明申请

US20180274006A1 METHOD FOR DETECTING MOLECULAR INTERACTIONS USING AN IMMOBILIZED NUCLEIC ACID 审中-公开
公开(公告)号：US20180274006A1
公开(公告)日：2018-09-27
申请号：US15764967
申请日：2016-09-29
申请人： DYNAMIC BIOSENSORS GMBH
发明人： Paul HAMPEL , Johannes REUSCH , Ulrich RANT , Ralf STRASSER
IPC分类号： C12Q1/6804 , G01N33/543 , G01N33/58 , G01N21/64
CPC分类号： C12Q1/6804 , C12Q1/6825 , G01N21/6428 , G01N27/3276 , G01N33/54353 , G01N33/582 , G01N33/68 , G01N2021/6439 , G01N2458/10 , C12Q2525/197 , C12Q2563/131 , C12Q2565/525 , C12Q2565/607
摘要： The present invention relates to a method for detecting and/or characterizing molecular interactions between two molecules, in particular two proteins and most particular between an antigen and an antibody by using an immobilized single-stranded nucleic acid molecule to which single-stranded nucleic acid molecules having a ligand attached thereto are hybridized.

7. 发明申请

US20180251829A1 MULTIPLEX Q-PCR ARRAYS 审中-公开
公开(公告)号：US20180251829A1
公开(公告)日：2018-09-06
申请号：US15972517
申请日：2018-05-07
申请人： California Institute of Technology
发明人： Arjang Hassibi , Babak Hassibi , Haris Vikalo
IPC分类号： C12Q1/6851 , G01N21/64 , C12Q1/686
CPC分类号： C12Q1/6825 , C12Q1/6834 , C12Q1/6851 , C12Q1/686 , C12Q2561/113 , C12Q2565/519 , G01N21/6408 , G01N21/6428 , G01N2021/6432 , G01N2021/6439 , C12Q2537/143 , C12Q2563/107 , C12Q2565/607 , C12Q2565/101
摘要： This invention provides methods and systems for measuring the concentration of multiple nucleic acid sequences in a sample. The nucleic acid sequences in the sample are simultaneously amplified, for example, using polymerase chain reaction (PCR) in the presence of an array of nucleic acid probes. The amount of amplicon corresponding to the multiple nucleic acid sequences can be measured in real-time during or after each cycle using a real-time microarray. The measured amount of amplicon produced can be used to determine the original amount of the nucleic acid sequences in the sample. Also provided herein are biosensor arrays, systems and methods for affinity based assays that are able to simultaneously obtain high quality measurements of the binding characteristics of multiple analytes, and that are able to determine the amounts of those analytes in solution. The invention also provides a fully integrated bioarray for detecting real-time characteristics of affinity based assays.

8. 发明申请

US20180238788A1 OPTICAL METHOD FOR TRACKING THE IN-VITRO AMPLIFICATION OF A NUCLEOTIDE SEQUENCE 审中-公开
公开(公告)号：US20180238788A1
公开(公告)日：2018-08-23
申请号：US15897895
申请日：2018-02-15
申请人： Commissariat A L'energie Atomique Et Aux Energies Alternatives
发明人： Cedric ALLIER , Melissa Baque , Thomas Bordy , Anne-Gaelle Bourdat , Daniel Vellou
IPC分类号： G01N15/02 , G01N15/14 , G01N33/53
CPC分类号： G01N15/0205 , C12Q1/6825 , C12Q1/6851 , G01N15/1468 , G01N21/272 , G01N21/4788 , G01N21/75 , G01N21/82 , G01N33/5304 , G01N2015/0057 , G01N2015/0065 , G01N2015/0092 , G01N2015/0222 , G01N2015/145 , G01N2021/755 , G03H2001/0447 , C12Q2525/301 , C12Q2563/137 , C12Q2565/301 , C12Q2565/607
摘要： The invention relates to a method for tracking the amplification of a sequence of nucleotides in a sample (10). The sample is placed between a light source (12) and an image sensor (16). Under the effect of amplification reagents, mixed with the sample, a nucleotide sequence, called the target sequence, is replicated iteratively, amplifying the target sequence. The method includes the acquisition of an image representative of the formation of a precipitate in the sample under the effect of the amplification, on the basis of which an image of interest is formed. The application of a statistical indicator to the image of interest allows an indicator of the amplification of the target sequence to be determined.

9. 发明申请

US20180216178A1 FABRICATION OF NANOCHANNEL WITH INTEGRATED ELECTRODES FOR DNA SEQUENCING USING TUNNELING CURRENT 审中-公开
公开(公告)号：US20180216178A1
公开(公告)日：2018-08-02
申请号：US15886511
申请日：2018-02-01
申请人： SEAGATE TECHNOLOGY LLC
发明人： Kim Yang LEE , Thomas Young CHANG , David S. KUO , ShuaiGang XIAO , Xiaomin YANG , Koichi WAGO
IPC分类号： C12Q1/6869 , G01N27/327 , G01N27/447 , B81C1/00
CPC分类号： C12Q1/6869 , B01L3/502707 , B01L2300/0645 , B01L2300/0816 , B01L2300/0887 , B01L2300/0896 , B81B2201/032 , B81B2201/058 , B81B2203/0109 , B81C1/00071 , G01N27/3278 , G01N27/447 , G01N27/44791 , C12Q2565/607 , C12Q2565/629
摘要： A DNA sequencing device and related methods, wherein the device includes a substrate, a nanochannel formed in the substrate, a first electrode positioned on a first side of the nanochannel, and a second electrode. The second electrode is positioned on a second side of the nanochannel opposite the first electrode, and is spaced apart from the first electrode to form an electrode gap that is exposed in the nanochannel. At least a portion of first electrode is movable relative to the second electrode to decrease a size of the electrode gap.

10. 发明授权

US10017810B2 Methods for determining a nucleotide sequence contiguous to a known target nucleotide sequence 有权
公开(公告)号：US10017810B2
公开(公告)日：2018-07-10
申请号：US15269448
申请日：2016-09-19
申请人： THE GENERAL HOSPITAL CORPORATION
发明人： Anthony John Iafrate , Long Phi Le , Zongli Zheng
IPC分类号： C12Q1/68 , C12Q1/6855 , C12Q1/6874 , C12Q1/6806
CPC分类号： C12Q1/6855 , C12Q1/6806 , C12Q1/6874 , C12Q2525/186 , C12Q2535/122 , C12Q2537/159 , C12Q2565/607
摘要： The technology described herein is directed to methods of determining oligonucleotide sequences, e.g. by enriching target sequences prior to sequencing the sequences.

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