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    • 3. 发明申请
    • DETERMINING A QUANTITY OF AN ANALYTE IN A BLOOD SAMPLE
    • 确定血样中分析物的数量
    • WO2017089599A1
    • 2017-06-01
    • PCT/EP2016/078916
    • 2016-11-25
    • ROCHE DIAGNOSTICS GMBHF. HOFFMANN-LA ROCHE AGROCHE DIAGNOSTICS OPERATIONS, INC.
    • BOEHM, ChristophBRUECKNER, ThorstenFISCHER, ThomasLOPEZ-CALLE, EloisaLUTZ, SaschaROEDL, JosefSPINKE, JuergenESPINDOLA, PamelaKELLER, Thomas ManuelDOLBINOW, ThomasADLER, SabrinaBEIERSDORF, ErikWENSORRA, Domenik
    • B01L3/00G01N35/00
    • B01L3/502753B01L3/5023B01L2200/0605B01L2200/16B01L2300/0803B01L2300/0864B01L2300/0867B01L2300/087B01L2400/0406B01L2400/0409B01L2400/0688B01L2400/084G01N35/00069G01N2035/1032
    • The invention provides for a medical system (700) for determining a quantity of an analyte (602, 602') in a blood sample (600) using a cartridge (100). The medical instrument comprises the cartridge. The cartridge is operable for being spun around a rotational axis (102), wherein the cartridge comprises: an inlet (108) for receiving the blood sample; a blood separation chamber (118) for separating blood plasma from the blood sample, wherein the blood separation chamber is fluidically connected to the inlet; a processing chamber (124) containing at least one reagent comprising at least one specific binding partner (611) which is operable to bind to the analyte to form at least one analyte specific binding partner complex; a first valve structure (122) connecting the blood separation chamber to the processing chamber; a measurement structure (130) for enabling measurement of the quantity of the analyte, wherein the measurement structure comprises a chromatographic membrane (134), wherein the chromatographic membrane comprises an immobilized binding partner (620) for direct or indirect binding of the analyte or the at least one analyte specific binding partner complex, wherein the measurement structure further comprises an absorbent structure (132), wherein the absorbent structure is nearer to the rotational axis than the membrane; a second valve structure (128) connecting the processing chamber to the measurement structure; and a fluid chamber (136, 136') filled with a washing buffer (1202), wherein the fluid chamber is fluidically connected to the measurement structure, wherein a seal keeps the washing buffer with the fluid chamber.
    • 本发明提供了一种使用药筒(100)确定血液样本(600)中的分析物(602,602')的量的医疗系统(700)。 医疗器械包括药筒。 药筒可操作用于围绕旋转轴线(102)旋转,其中药筒包括:入口(108),用于接收血液样本; 血液分离室(118),用于从血液样品中分离血浆,其中血液分离室流体连接到入口; 包含至少一种试剂的处理室(124),所述试剂包含至少一个特异性结合配偶体(611),所述特定结合配偶体可操作以结合所述分析物以形成至少一种分析物特异性结合配偶体复合物; 将血液分离室连接到处理室的第一阀结构(122) 测量结构(130),用于能够测量分析物的量,其中测量结构包括色谱膜(134),其中色谱膜包括固定的结合配偶体(620),用于直接或间接结合分析物或 至少一种分析物特异性结合配偶体复合物,其中所述测量结构还包括吸收性结构(132),其中所述吸收性结构比所述膜更靠近所述旋转轴线; 将处理腔室连接到测量结构的第二阀结构(128) 和填充有清洗缓冲液(1202)的流体腔室(136,136'),其中所述流体腔室流体连接到所述测量结构,其中密封件保持所述清洗缓冲液与所述流体腔室的连接。