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    • 4. 发明申请
    • ZELLKULTURANLAGE ZUR KULTIVIERUNG ADHÄRENTER ZELLEN SOWIE FLUID-VERSORGUNGSSCHNITTSTELLE MIT ZELLKULTURBEHÄLTER
    • 的培养体系细胞培养容器培育细胞和供液附着界面
    • WO2014114610A1
    • 2014-07-31
    • PCT/EP2014/051072
    • 2014-01-21
    • HAMILTON BONADUZ AG
    • JÄGER, ThomasSCHLENKER, DirkOTHMAN, NabihKÜHNE, Oliver
    • C12M1/00C12M1/26C12M1/34
    • C12M41/44C12M23/40C12M29/00C12M33/07C12M37/04C12M39/00C12M41/00
    • Die Erfindung betrifft eine Fluid-Versorgungsschnittstelle (62; 62'; 62"; 62"') für eine Zellkulturanlage zur Versorgung von in unterschiedlichen Zellkulturbehältern (10; 10'; 10") vorhandenen Zellkulturen mit einem Nährmedium, wobei die Fluid-Versorgungsschnittstelle (62; 62'; 62"; 62"') umfasst: ein einen Strömungsraum (72) definierendes Gehäuse (68), eine erste Anschlussformation (76) zur fluidübertragenden Verbindung einer ersten Fluidleitung (84) mit dem Gehäuse (68), eine von der ersten gesondert ausgebildete zweite Anschlussformation (78) zur fluidübertragenden Verbindung einer zweiten Fluidleitung (88) mit dem Gehäuse (68), eine von den ersten beiden gesondert ausgebildete dritte Anschlussformation (80) zur fluidübertragenden Verbindung des Gehäuses (68) mit einer dritten Fluidleitung, eine von den Anschlussformationen (76, 78, 80) gesondert ausgebildete Kopplungsformation (64, 66), welche zum betriebsmäßig herstellbaren und lösbaren fluidübertragenden Kopplungseingriff mit einer entsprechenden Gegenkopplungsformation (38, 40) eines Zellkulturbehälters (10; 10'; 10") ausgebildet ist.
    • 本发明涉及一种流体供给接口(62; 62“; 62‘; 62’”),用于细胞培养系统中不同的细胞培养容器来提供(10; 10“; 10“)的现有的细胞培养用的营养培养基,其中,所述流体供给接口( 62; 62“; 62‘; 62’”)包括:限定了用于流体传递连接的壳体(68),第一连接形成(76)到第一流体导管(84)到外壳(68)的,一个流动室(72) 第一单独形成第二连接形成(78),用于与第二流体管线(88)到外壳(68)的流体传递连接,一个分开的壳体(68)具有一个第三流体导管流体传递连接前两个第三连接形成(80)形成, 该连接结构中的一个(76,78,80)分开地形成耦合结构(64,66),用于可操作地和可释放地可生产流体传动Kopplungse ingriff与细胞培养容器的相应的对耦合的形成(38,40)(10; 10“; 10“)形成。
    • 7. 发明申请
    • AUTOMATED CELL CULTURE SYSTEM AND METHOD
    • 自动细胞培养系统及方法
    • WO2015023658A2
    • 2015-02-19
    • PCT/US2014/050704
    • 2014-08-12
    • INVIVOSCIENCES INC.
    • CONWAY, MichailkGERGER, MichaelWAKUTSUKI, Tetsuro
    • C12M3/04
    • C12M23/12C12M33/06C12M33/07C12M41/12C12M41/36C12M41/48
    • An automated method for culturing stem cells using a robotic liquid handling system including a translatable bed and a movable multi-channel pipette. The method includes the steps of: locating a first multi-well cell culture plate and a multi-trough plate on the bed; placing a suspension of stem cells in at least one trough of the multi-trough plate; using the multi-channel pipette, transferring a portion of the suspension of stem cells to each well of the first multi-well cell culture plate such that at least two of the wells of the first multi-well cell culture plate have different densities of stem cells; selecting a well of the first multi-well cell culture plate having a desired density of stem cells; locating a second multi-well cell culture plate on the bed; and using the multi-channel pipette, transferring the cells of the selected well to a plurality of wells of the second multi-well cell culture plate.
    • 一种使用包括可翻译床和可移动多通道移液管的机器人液体处理系统培养干细胞的自动化方法。 该方法包括以下步骤:在床上定位第一多孔细胞培养板和多槽板; 将干细胞的悬浮液放置在多槽板的至少一个槽中; 使用多通道移液管,将一部分干细胞的悬浮液转移到第一多孔细胞培养板的每个孔中,使得第一多孔细胞培养板的至少两个孔具有不同密度的茎 细胞; 选择具有所需密度的干细胞的第一多孔细胞培养板的孔; 在床上放置第二个多孔细胞培养板; 以及使用所述多通道移液管,将所选孔的细胞转移到所述第二多孔细胞培养板的多个孔中。
    • 9. 发明申请
    • DEVICE FOR TAKING UP AND TRANSFERRING BIOLOGICAL SAMPLES
    • 设备记录和生物样品的转移
    • WO99066023A2
    • 1999-12-23
    • PCT/EP1999/004161
    • 1999-06-15
    • B01L3/00C12M1/32
    • B01L3/5085C12M33/07
    • The invention relates to a device (2) for the targeted uptake of biological samples (e.g. 46, 48) and the transfer thereof into predetermined compartments (50) of a microtitration plate (52) by means of a corresponding number of needle-shaped sample uptake elements (14) which can be moved in a controlled manner. The inventive device is characterized in that the sample uptake elements are part of a flexible band (4) which can be progressively advanced and extracted by means of a switching and guiding device (6) which can be hand held. Said elements are arranged in said band such that they are perpendicular to the direction of advancement and such that they are adjacent in a manner similar to the teeth of a comb. In addition, the elements are arranged at distances which correspond to the distances of the compartments (50) of each row of the utilized microtitration plates (52). The inventive device is also characterized in that the switching and guiding devices (6) can divert the band (4) in such a way that, in each step switching position of the band, each one of the sample uptake elements projects outward with the tip thereof from the row of the remaining sample uptake elements. By using the device (2), the individual sample uptake elements (14) can be individually loaded in a successive manner with the samples to be taken up. Said band (4) is withdrawn from the switching and guiding device (6) and is dipped into a row of compartments (50) of the microtitration plate (52) in extended form with the sample uptake elements (14) thereof.
    • 通过可动针状样品拾取元件的相应数量(14)的装置在微量滴定板(52)的预定的隔室(50)来控制用于生物样品的靶向成像的设备(2)(例如46,48)和它们的传输,其特征在于所述样品接收元件的一部分 通过手持式开关和引导装置的装置(6)逐渐继续切换的和从可移除的柔性带(4),横向于所述分度方向,在梳状的齿的方式并置的,在对应于所述腔室的间距(50),其中它们各自的间距 使用(52)的微量滴定板的一排被布置,并且,所述开关和引导装置(6),其能够以这样的方式偏转所述带(4),在每种情况下与从剩余样品拾取元件排其尖端样品接收元件中的一个在磁带的每一步进位置 出现在外面。 使得各个样品接纳元件(14)可以连续地单独地与雄性样品的由(2),于是所讨论的胶带(4)的开关和引导装置(6)被去除的设备的装置和在拉伸形式与它的样品接收元件(14)装 在一个数的微量滴定板的腔室(50),(52)浸入。