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1. 发明申请

WO2009135212A2 SELECTIVE 5' LIGATION TAGGING OF RNA 审中-公开
标题翻译：选择性5'RNA结合标记
公开(公告)号：WO2009135212A2
公开(公告)日：2009-11-05
申请号：PCT/US2009042723
申请日：2009-05-04
申请人： JENDRISAK JEROME J , VAIDYANATHAN RAMESH , DAHL GARY , EPICT TECHNOLOGIES CORP
发明人： JENDRISAK JEROME J , VAIDYANATHAN RAMESH , DAHL GARY
IPC分类号： C12N15/10 , C07H21/02 , C12N15/11 , C12Q1/68
CPC分类号： C12Q1/6806 , C12N15/1096 , C12Q1/6855 , C12Q2525/207 , C12Q2525/125 , C12Q2521/525
摘要： The present invention provides novel compositions, kits and methods employing RNA 5' polyphosphatases, RNA 5' monophosphatases, capping enzymes, decapping enzymes, nucleic acid pyrophosphatases and RNA ligases, as well as other enzymes, for selective 5' ligation tagging of desired classes of RNA molecules that differ with respect to particular chemical moieties on their 5' ends. The 5'tagged RNA molecules can be used for synthesis of tagged first-stand cDNA, double-stranded cDNA, and sense or antisense RNA for a variety of uses.
摘要翻译：本发明提供了使用RNA 5'多磷酸酶，RNA 5'单磷酸酶，加帽酶，脱帽酶，核酸焦磷酸酶和RNA连接酶以及其他酶的新型组合物，试剂盒和方法，用于选择性5'连接标记所需类别的 RNA分子就其5'端的特定化学部分而言不同。 5'标签的RNA分子可用于各种用途的标记的第一站cDNA，双链cDNA和有义或反义RNA的合成。

2. 发明申请

WO2009006438A3 COPY DNA AND SENSE RNA 审中-公开
标题翻译：复制DNA和感染RNA
公开(公告)号：WO2009006438A3
公开(公告)日：2009-02-26
申请号：PCT/US2008068844
申请日：2008-06-30
申请人： EPICT TECHNOLOGIES CORP , DAHL GARY , SOOKNANAN ROY RABINDRANAUTH
发明人： DAHL GARY , SOOKNANAN ROY RABINDRANAUTH
IPC分类号： C12N15/09 , C07H21/04 , C12P19/34 , C12Q1/68
CPC分类号： C12N15/1096 , C12Q1/6865 , C12Q2525/186 , C12Q2525/155
摘要： The present invention relates generally to methods, compositions and kits for synthesizing sense RNA molecules from one or more RNA molecules of interest in a sample. In exemplary embodiments, the methods use a terminal tagging oligoribonucleotide (rTTO) to join a DNA sequence tag to the 3'-termini of first-strand cDNA molecules. The use of an rTTO comprising ribonucleotides results in decreased oligonucleotide-derived background synthesis of RNA in the absence of sample RNA and, surprisingly and unexpectedly, also results in significantly increased yields of sense RNA molecules that exhibit sequences that are substantially identical to those of the RNA molecules of interest in the sample. The sense RNA molecules also have an RNA sequence tag on their 5 '-termini that is useful for fixing the lengths of sense RNA molecules that are synthesized in a second or subsequent round.
摘要翻译：本发明一般涉及从样品中一个或多个感兴趣的RNA分子合成有义RNA分子的方法，组合物和试剂盒。在示例性实施方案中，所述方法使用末端标记寡核糖核苷酸（rTTO）将DNA序列标签连接到第一链cDNA分子的3'-末端。包含核糖核苷酸的rTTO的使用导致在不存在样品RNA的情况下寡核苷酸衍生的RNA背景合成减少，令人惊讶地和出人意料地也导致显着提高正义RNA分子的产量，其显示出与样品中感兴趣的RNA分子。正义RNA分子在其5'末端也具有RNA序列标签，其可用于固定在第二次或随后的一轮中合成的有义RNA分子的长度。

3. 发明申请

WO2011071931A2 RNA PREPARATIONS COMPRISING PURIFIED MODIFIED RNA FOR REPROGRAMMING CELLS 审中-公开
标题翻译：包含用于转染细胞的纯化的修饰的RNA的RNA制备
公开(公告)号：WO2011071931A2
公开(公告)日：2011-06-16
申请号：PCT/US2010059305
申请日：2010-12-07
申请人： KARIKO KATALIN , WEISSMAN DREW , DAHL GARY , PERSON ANTHONY , MEIS JUDITH , JENDRISAK JEROME
发明人： KARIKO KATALIN , WEISSMAN DREW , DAHL GARY , PERSON ANTHONY , MEIS JUDITH , JENDRISAK JEROME
IPC分类号： C12N15/113 , C12N5/02 , C12N5/071
CPC分类号： C12N5/0696 , A61K38/1709 , A61K38/1816 , A61K38/44 , A61K38/465 , A61K38/50 , A61K48/005 , A61K48/0075 , C07K14/47 , C07K14/4712 , C07K14/505 , C12N9/0075 , C12N9/22 , C12N9/78 , C12N15/117 , C12N15/85 , C12N15/87 , C12N2310/17 , C12N2310/335 , C12N2320/30 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2501/606 , C12N2501/608 , C12N2506/02 , C12Y114/13039 , C12Y301/04012 , C12Y305/04004
摘要： The present invention provides compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single-strand mRNA encoding an iPS cell induction factor. The purified RNA preparations are preferably substantially free of RNA contaminant molecules that: i) would activate an immune response in the somatic cells, ii) would decrease expression of the single-stranded mRNA in the somatic cells, and/or iii) active RNA sensors in the somatic cells. In certain embodiments, the purified RNA preparations are substantially free of partial mRNAs, double-stranded RNAs, un-capped RNA molecules, and/or single-stranded run-on mRNAs.
摘要翻译：本发明提供使用包含编码iPS细胞诱导因子的单链mRNA的纯化RNA制备物重编程体细胞的组合物和方法。纯化的RNA制剂优选基本上不含RNA污染物分子，其可以：i）激活体细胞中的免疫应答，ii）降低体细胞中单链mRNA的表达，和/或iii）活性RNA传感器在体细胞中。在某些实施方案中，纯化的RNA制剂基本上不含部分mRNA，双链RNA，未封端的RNA分子和/或单链连续的mRNA。

4. 发明申请

WO2007120863A3 KITS AND METHODS FOR GENERATING 5' CAPPED RNA 审中-公开
标题翻译：用于产生5'CAPPED RNA的试剂盒和方法
公开(公告)号：WO2007120863A3
公开(公告)日：2008-10-30
申请号：PCT/US2007009217
申请日：2007-04-16
申请人： EPICT TECHNOLOGIES , JENDRISAK JEROME , MEIS RONALD , DAHL GARY
发明人： JENDRISAK JEROME , MEIS RONALD , DAHL GARY
IPC分类号： C12N15/11 , C12N15/113 , C12N15/85 , C12P19/34
CPC分类号： C12P19/34 , C12N9/1007 , C12N9/1241 , C12N15/1072 , C12N15/1075 , C12N15/1096 , C12N15/111 , C12N15/113 , C12N2310/317 , C12N2320/51 , C12Y201/01056 , C12Y201/01057 , C12Y207/07019 , C12Y207/0705
摘要： The present invention relates to kits and methods for efficiently generating 5' capped RNA having a modified cap nucleotide and for use of such modified-nucleotide-capped RNA molecules. The invention is used to obtain novel compositions of such modified- nucleotide-capped RNA molecules. In particular, the present invention provides kits and methods for capping RNA using a modified cap nucleotide and a capping enzyme system, such as poxvirus capping enzyme. The present invention finds use for in vitro production o 5'-capped RNA having a modified cap nucleotide and for in vitro or in vivo production o polypeptides by in vitro or in vivo translation of such modified-nucleotide-capped RNA for a variety of research, therapeutic, and commercial applications. The invention also provides methods and kits for capturing or isolating uncapped RNA comprising primary RNA transcripts or RNA having a 5'-diphosphate, such as RNA synthesized in vitro or obtained from a biological source, including prokaryotic mRNA that is in a mixture with other prokaryotic and/or eukaryotic nucleic acids. The method for capturing modified-nucleotide- capped RNA also provides methods and kits for obtaining only type-specific or condition- specific modified-nucleotide-capped RNA by cap-dependent subtraction of that portion of the captured modified-nucleotide-capped RNA in cells of one type or condition that is the same as RNA in cells of another type or condition. The invention further provides methods and kits for using a capping enzyme system and modified cap nucleotides for labelin uncapped RNA comprising primary RNA transcripts or RNA having a 5 '-diphosphate with detectable dye or enzyme moieties.
摘要翻译：本发明涉及用于有效产生具有修饰的帽核苷酸的5'封端RNA并用于这种修饰的核苷酸封端RNA分子的试剂盒和方法。本发明用于获得这种经修饰的核苷酸封端RNA分子的新组合物。特别地，本发明提供了使用修饰的帽核苷酸和封端酶系统（例如痘病毒覆盖酶）对RNA进行封端的试剂盒和方法。本发明用于体外生产o具有修饰的帽核苷酸的体外或体内生产的5'-封端RNA，通过体外或体内翻译这些经修饰的核苷酸封端的RNA进行多种研究，治疗和商业应用。本发明还提供用于捕获或分离未封端RNA的方法和试剂盒，其包含初级RNA转录物或具有5'-二磷酸的RNA，例如体外合成或从生物来源获得的RNA，包括与其他原核生物混合的原核mRNA 和/或真核的核酸。用于捕获修饰的核苷酸封端RNA的方法还提供了通过在细胞中捕获的修饰的核苷酸加帽的RNA的那部分的帽依赖性减法来获得仅特定类型或条件特异性修饰的核苷酸加帽的RNA的方法和试剂盒与另一种类型或病症细胞中的RNA相同的一种类型或病症。本发明还提供了用于使用封端酶系统和修饰的帽子核苷酸用于标记未封端RNA的方法和试剂盒，其包含具有可检测染料或酶部分的5'-二磷酸的初级RNA转录物或RNA。

5. 发明申请

WO2011071936A3 COMPOSITIONS AND METHODS FOR REPROGRAMMING EUKARYOTIC CELLS 审中-公开
标题翻译：复制原核细胞的组合物和方法
公开(公告)号：WO2011071936A3
公开(公告)日：2011-10-13
申请号：PCT/US2010059317
申请日：2010-12-07
申请人： DAHL GARY , PERSON ANTHONY , MEIS JUDITH , JENDRISAK JEROME
发明人： DAHL GARY , PERSON ANTHONY , MEIS JUDITH , JENDRISAK JEROME
IPC分类号： C12N5/074 , C12N15/113
CPC分类号： C12N5/0696 , A61K38/1709 , A61K38/1816 , A61K38/44 , A61K38/465 , A61K38/50 , A61K48/005 , A61K48/0075 , C07K14/47 , C07K14/4712 , C07K14/505 , C12N9/0075 , C12N9/22 , C12N9/78 , C12N15/117 , C12N15/85 , C12N15/87 , C12N2310/17 , C12N2310/335 , C12N2320/30 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2501/606 , C12N2501/608 , C12N2506/02 , C12Y114/13039 , C12Y301/04012 , C12Y305/04004
摘要： The present invention relates to methods for changing the state of differentiation of a eukaryotic cell, the methods comprising introducing mRNA encoding one or more reprogramming factors into a cell and maintaining the cell under conditions wherein the cell is viable and the mRNA that is introduced into the cell is expressed in sufficient amount and for sufficient time to generate a cell that exhibits a changed state of differentiation compared to the cell into which the mRNA was introduced, and compositions therefor. For example, the present invention provides mRNA molecules and methods for their use to reprogram human somatic cells into pluripotent stem cells.
摘要翻译：本发明涉及用于改变真核细胞分化状态的方法，所述方法包括将编码一种或多种重编程因子的mRNA引入细胞并将细胞维持在其中细胞存活的条件下，并将导入表达足够的量和足够的时间以产生与引入mRNA的细胞相比表现出改变的分化状态的细胞及其组合物。例如，本发明提供mRNA分子及其用于将人体细胞重编程为多能干细胞的方法。

6. 发明申请

WO2006037102A3 METHODS FOR IDENTIFYING POLYMERASE INHIBITORS 审中-公开
标题翻译：鉴定聚合酶抑制剂的方法
公开(公告)号：WO2006037102A3
公开(公告)日：2007-12-27
申请号：PCT/US2005035017
申请日：2005-09-27
申请人： EPICT TECHNOLOGIES , JENDRISAK JERRY , RADEK AGNES , DAHL GARY
发明人： JENDRISAK JERRY , RADEK AGNES , DAHL GARY
IPC分类号： C12Q1/68
CPC分类号： C12Q1/48 , C12Q1/68 , G01N2333/9125 , G01N2500/00 , C12Q2527/127
摘要： The present invention relates to methods and compositions for the identification of enzyme inhibitors. In particular, the present invention relates to the identification of nucleic acid polymerase inhibitors.
摘要翻译：本发明涉及用于鉴定酶抑制剂的方法和组合物。特别地，本发明涉及核酸聚合酶抑制剂的鉴定。

7. 发明申请

WO2006086668A2 COMPOSITIONS AND METHODS EMPLOYING 5'-PHOSPHATE-DEPENDENT NUCLEIC ACID EXONUCLEASES 审中-公开
标题翻译：使用5'-磷酸依赖性核酸脱氢酶的组合物和方法
公开(公告)号：WO2006086668A2
公开(公告)日：2006-08-17
申请号：PCT/US2006004798
申请日：2006-02-09
申请人： EPICT TECHNOLOGIES , JENDRISAK JEROME , MEIS JUDITH , MEIS RONALD , RADEK AGNES , DAHL GARY
发明人： JENDRISAK JEROME , MEIS JUDITH , MEIS RONALD , RADEK AGNES , DAHL GARY
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12N15/1096 , C12Q1/6806 , C12Q2521/319
摘要： The present invention relates to compositions and methods employing 5'- phosphate-dependent nucleic acid exonucleases. In particular, the present invention provides kits and methods employing 5 '-phosphate-dependent nucleic acid exonucleases for selective enrichment, isolation and amplification of a particular set of desired nucleic acid molecules from samples that also contain undesired nucleic acid molecules for a variety of uses. In preferred embodiments, the desired nucleic acid molecules comprise prokaryotic and/or eukaryotic mRNA.
摘要翻译：本发明涉及使用5'-磷酸依赖性核酸外切核酸酶的组合物和方法。特别地，本发明提供了使用5'-磷酸依赖性核酸外切核酸酶用于选择性富集，分离和扩增特定的一组所需核酸分子的试剂盒和方法，该样品还含有用于各种用途的不需要的核酸分子的样品。在优选的实施方案中，所需的核酸分子包含原核和/或真核的mRNA。

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