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1. 发明申请

WO2007143206A3 COMPOSITIONS AND METHODS FOR REMOVAL OF DNA FROM A SAMPLE 审中-公开
标题翻译：从样品中去除DNA的组合物和方法
公开(公告)号：WO2007143206A3
公开(公告)日：2008-10-09
申请号：PCT/US2007013184
申请日：2007-06-04
申请人： EPICT TECHNOLOGIES , JENDRISAK JEROME , GRUNENWALD HAIYING L
发明人： JENDRISAK JEROME , GRUNENWALD HAIYING L
IPC分类号： G01N33/53
CPC分类号： C12N9/22 , C12N1/08
摘要： The present invention provides compositions and methods for digesting DNA. In particular, the present invention provides enzymes mixtures that provide enhanced DNA digestion and methods of using the enzyme mixtures to eliminate or reduce undesired DNA molecules from a sample of interest.
摘要翻译：本发明提供用于消化DNA的组合物和方法。特别地，本发明提供了提供增强的DNA消化的酶混合物，以及使用酶混合物从感兴趣的样品中消除或减少不想要的DNA分子的方法。

2. 发明申请

WO2009135214A3 RNA POLYPHOSPHATASE COMPOSITIONS, KITS, AND USES THEREOF 审中-公开
标题翻译： RNA多磷酸酯组合物，胶囊及其用途
公开(公告)号：WO2009135214A3
公开(公告)日：2010-03-11
申请号：PCT/US2009042729
申请日：2009-05-04
申请人： JENDRISAK JEROME J , VAIDYANATHAN RAMESH , MEIS RONALD , EPICT TECHNOLOGIES CORP
发明人： JENDRISAK JEROME J , VAIDYANATHAN RAMESH , MEIS RONALD
IPC分类号： C12N9/14 , C12Q1/42 , C12Q1/68
CPC分类号： C12Q1/42 , C12N9/14 , C12P19/34 , Y10T436/143333
摘要： The present invention relates to the discovery of RNA 5' polyphosphatase enzymes not previously described in the art, methods for discovery of said enzymes, compositions of said enzymes, methods for making said enzymes, and various methods and kits for using said enzymes for biomedical research, for human and non-human diagnostics, for production of therapeutic products, and for other applications. In particular, some embodiments provide compositions, kits and methods for employing RNA polyphosphatases for isolation, purification, production, and assay of capped RNA using a biological sample or a sample from an in vitro capping reaction wherein the sample also contains RNA that is not capped. Other embodiments provide compositions, kits and methods wherein RNA polyphosphatases comprise signal-amplifying enzymes for analyte-specific assays.
摘要翻译：本发明涉及本领域先前未描述的RNA 5'多磷酸酶的发现，所述酶的发现方法，所述酶的组合物，所述酶的制备方法以及使用所述酶进行生物医学研究的各种方法和试剂盒，用于人和非人诊断，用于生产治疗产品和其他应用。具体地，一些实施方案提供了使用RNA多磷酸酶用于使用生物样品或来自体外封端反应的样品分离，纯化，生产和测定加帽RNA的组合物，试剂盒和方法，其中所述样品还含有未被封端的RNA 。其他实施方案提供组合物，试剂盒和方法，其中RNA多磷酸酶包含用于分析物特异性测定的信号放大酶。

3. 发明申请

WO2009006438A3 COPY DNA AND SENSE RNA 审中-公开
标题翻译：复制DNA和感染RNA
公开(公告)号：WO2009006438A3
公开(公告)日：2009-02-26
申请号：PCT/US2008068844
申请日：2008-06-30
申请人： EPICT TECHNOLOGIES CORP , DAHL GARY , SOOKNANAN ROY RABINDRANAUTH
发明人： DAHL GARY , SOOKNANAN ROY RABINDRANAUTH
IPC分类号： C12N15/09 , C07H21/04 , C12P19/34 , C12Q1/68
CPC分类号： C12N15/1096 , C12Q1/6865 , C12Q2525/186 , C12Q2525/155
摘要： The present invention relates generally to methods, compositions and kits for synthesizing sense RNA molecules from one or more RNA molecules of interest in a sample. In exemplary embodiments, the methods use a terminal tagging oligoribonucleotide (rTTO) to join a DNA sequence tag to the 3'-termini of first-strand cDNA molecules. The use of an rTTO comprising ribonucleotides results in decreased oligonucleotide-derived background synthesis of RNA in the absence of sample RNA and, surprisingly and unexpectedly, also results in significantly increased yields of sense RNA molecules that exhibit sequences that are substantially identical to those of the RNA molecules of interest in the sample. The sense RNA molecules also have an RNA sequence tag on their 5 '-termini that is useful for fixing the lengths of sense RNA molecules that are synthesized in a second or subsequent round.
摘要翻译：本发明一般涉及从样品中一个或多个感兴趣的RNA分子合成有义RNA分子的方法，组合物和试剂盒。在示例性实施方案中，所述方法使用末端标记寡核糖核苷酸（rTTO）将DNA序列标签连接到第一链cDNA分子的3'-末端。包含核糖核苷酸的rTTO的使用导致在不存在样品RNA的情况下寡核苷酸衍生的RNA背景合成减少，令人惊讶地和出人意料地也导致显着提高正义RNA分子的产量，其显示出与样品中感兴趣的RNA分子。正义RNA分子在其5'末端也具有RNA序列标签，其可用于固定在第二次或随后的一轮中合成的有义RNA分子的长度。

4. 发明申请

WO2006037102A3 METHODS FOR IDENTIFYING POLYMERASE INHIBITORS 审中-公开
标题翻译：鉴定聚合酶抑制剂的方法
公开(公告)号：WO2006037102A3
公开(公告)日：2007-12-27
申请号：PCT/US2005035017
申请日：2005-09-27
申请人： EPICT TECHNOLOGIES , JENDRISAK JERRY , RADEK AGNES , DAHL GARY
发明人： JENDRISAK JERRY , RADEK AGNES , DAHL GARY
IPC分类号： C12Q1/68
CPC分类号： C12Q1/48 , C12Q1/68 , G01N2333/9125 , G01N2500/00 , C12Q2527/127
摘要： The present invention relates to methods and compositions for the identification of enzyme inhibitors. In particular, the present invention relates to the identification of nucleic acid polymerase inhibitors.
摘要翻译：本发明涉及用于鉴定酶抑制剂的方法和组合物。特别地，本发明涉及核酸聚合酶抑制剂的鉴定。

5. 发明申请

WO2006086668A2 COMPOSITIONS AND METHODS EMPLOYING 5'-PHOSPHATE-DEPENDENT NUCLEIC ACID EXONUCLEASES 审中-公开
标题翻译：使用5'-磷酸依赖性核酸脱氢酶的组合物和方法
公开(公告)号：WO2006086668A2
公开(公告)日：2006-08-17
申请号：PCT/US2006004798
申请日：2006-02-09
申请人： EPICT TECHNOLOGIES , JENDRISAK JEROME , MEIS JUDITH , MEIS RONALD , RADEK AGNES , DAHL GARY
发明人： JENDRISAK JEROME , MEIS JUDITH , MEIS RONALD , RADEK AGNES , DAHL GARY
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12N15/1096 , C12Q1/6806 , C12Q2521/319
摘要： The present invention relates to compositions and methods employing 5'- phosphate-dependent nucleic acid exonucleases. In particular, the present invention provides kits and methods employing 5 '-phosphate-dependent nucleic acid exonucleases for selective enrichment, isolation and amplification of a particular set of desired nucleic acid molecules from samples that also contain undesired nucleic acid molecules for a variety of uses. In preferred embodiments, the desired nucleic acid molecules comprise prokaryotic and/or eukaryotic mRNA.
摘要翻译：本发明涉及使用5'-磷酸依赖性核酸外切核酸酶的组合物和方法。特别地，本发明提供了使用5'-磷酸依赖性核酸外切核酸酶用于选择性富集，分离和扩增特定的一组所需核酸分子的试剂盒和方法，该样品还含有用于各种用途的不需要的核酸分子的样品。在优选的实施方案中，所需的核酸分子包含原核和/或真核的mRNA。

6. 发明申请

WO2011056866A2 METHODS AND KITS FOR 3'-END-TAGGING OF RNA 审中-公开
标题翻译： RNA的3'末端标记的方法和工具
公开(公告)号：WO2011056866A2
公开(公告)日：2011-05-12
申请号：PCT/US2010055292
申请日：2010-11-03
申请人： EPICT TECHNOLOGIES CORP , VAIDYANATHAN RAMESH , KUERSTEN SCOTT , DOYLE KEN
发明人： VAIDYANATHAN RAMESH , KUERSTEN SCOTT , DOYLE KEN
IPC分类号： C12Q1/68 , C12N15/10 , C12N15/11
CPC分类号： C12Q1/6806 , C12N15/10 , C12N15/1096 , C12N15/66 , C12P19/34
摘要： The present innovation provides methods and kits that enable rapid and efficient dual end-tagging of RNA to prepare libraries for analysis by applications such as next-generation RNA sequencing, qPCR, microarray analysis, or cloning. The methods do not require time-consuming and inefficient gel-purification steps that are common to methods known in the art. In addition, the present invention provides methods and kits for rapid, high-throughput enzymatic preparation of 5'-activated, 3'-blocked DNA oligonucleotides from standard, single-stranded DNA oligonucleotides.
摘要翻译：本创新提供了方法和试剂盒，可以快速有效的双端标记RNA，以制备用于分析应用的文库，如下一代RNA测序，qPCR，微阵列分析或克隆。该方法不需要本领域已知方法常见的耗时和低效的凝胶纯化步骤。此外，本发明提供了用于从标准的单链DNA寡核苷酸快速，高通量酶制备5'-激活的，3'-封闭的DNA寡核苷酸的方法和试剂盒。

7. 发明申请

WO2009135212A2 SELECTIVE 5' LIGATION TAGGING OF RNA 审中-公开
标题翻译：选择性5'RNA结合标记
公开(公告)号：WO2009135212A2
公开(公告)日：2009-11-05
申请号：PCT/US2009042723
申请日：2009-05-04
申请人： JENDRISAK JEROME J , VAIDYANATHAN RAMESH , DAHL GARY , EPICT TECHNOLOGIES CORP
发明人： JENDRISAK JEROME J , VAIDYANATHAN RAMESH , DAHL GARY
IPC分类号： C12N15/10 , C07H21/02 , C12N15/11 , C12Q1/68
CPC分类号： C12Q1/6806 , C12N15/1096 , C12Q1/6855 , C12Q2525/207 , C12Q2525/125 , C12Q2521/525
摘要： The present invention provides novel compositions, kits and methods employing RNA 5' polyphosphatases, RNA 5' monophosphatases, capping enzymes, decapping enzymes, nucleic acid pyrophosphatases and RNA ligases, as well as other enzymes, for selective 5' ligation tagging of desired classes of RNA molecules that differ with respect to particular chemical moieties on their 5' ends. The 5'tagged RNA molecules can be used for synthesis of tagged first-stand cDNA, double-stranded cDNA, and sense or antisense RNA for a variety of uses.
摘要翻译：本发明提供了使用RNA 5'多磷酸酶，RNA 5'单磷酸酶，加帽酶，脱帽酶，核酸焦磷酸酶和RNA连接酶以及其他酶的新型组合物，试剂盒和方法，用于选择性5'连接标记所需类别的 RNA分子就其5'端的特定化学部分而言不同。 5'标签的RNA分子可用于各种用途的标记的第一站cDNA，双链cDNA和有义或反义RNA的合成。

8. 发明申请

WO2004058989A3 TARGET-DEPENDENT TRANSCRIPTION 审中-公开
标题翻译：目标相关转录
公开(公告)号：WO2004058989A3
公开(公告)日：2007-10-18
申请号：PCT/US0340912
申请日：2003-12-23
申请人： EPICT TECHNOLOGIES , DAHL GARY A , JENDRISAK JEROME J , MEIS JUDY E , VAIDYANATHAN RAMESH
IPC分类号： C12Q1/68 , C07H21/04 , C12N9/00 , C12P19/34 , C12Q20060101 , C12Q1/00 , G01N20060101
CPC分类号： C12Q1/6862 , C12Q1/682 , C12Q1/6865 , C12Q2600/156 , C12Q2531/137 , C12Q2531/125 , C12Q2525/161 , C12Q2525/143
摘要： The present invention comprises novel methods, compositions and kits comprising monopartite or bipartite target probes and an RNA polymerase to detect and quantify analytes comprising one or multiple target nucleic acid sequences, including target sequences that differ by as little as one nucleotide, or to detect and quantify non-nucleic acid analytes by detecting a target sequence tag that is joined to an analyte-binding substance. The method, called "targetdependent transcription," consists of an annealing process, a DNA ligation process, a transcription process, and a detection process. The invention also comprises novel methods, compositions and kits for amplifying RNA, including strand-displacement reverse transcription and rolling circle reverse transcription.
摘要翻译：本发明包括新颖的方法，组合物和试剂盒，其包括单分子或双分子靶标探针和RNA聚合酶，用于检测和定量包含一个或多个靶核酸序列的分析物，包括不同于一个核苷酸的靶序列，或检测和通过检测连接到分析物结合物质的靶序列标签来定量非核酸分析物。称为“靶依赖性转录”的方法由退火过程，DNA连接过程，转录过程和检测过程组成。本发明还包括用于扩增RNA的新方法，组合物和试剂盒，包括链 - 位移逆转录和滚环反转录。

9. 发明申请

WO2004048596A3 METHODS FOR USING PRIMERS THAT ENCODE ONE STRAND OF A DOUBLE-STRANDED PROMOTER 审中-公开
标题翻译：使用一个双向拉伸推进器的一个条纹的方法
公开(公告)号：WO2004048596A3
公开(公告)日：2004-08-26
申请号：PCT/US0337454
申请日：2003-11-21
申请人： EPICT TECHNOLOGIES , DAHL GARY A , JENDRISAK JEROME J
发明人： DAHL GARY A , JENDRISAK JEROME J
IPC分类号： C12P19/34 , C12Q1/68 , C07H21/04
CPC分类号： C12N15/1096 , C12Q1/6853 , C12Q2531/119 , C12Q2521/301 , C12Q2525/125
摘要： The present invention provides methods, compositions and kits for using an RNA polymerase for making transcription products corresponding to a target sequence by obtaining circular single-stranded DNA transcription substrates using a promoter primer that encodes one strand of a double-stranded promoter. The invention has broad applicability for research, diagnostic and therapeutic applications, such as preparing cDNA corresponding to mRNA, making sense or anti-sense probes, detecting gene- or organism-specific sequences, or making RNAi.
摘要翻译：本发明提供使用RNA聚合酶制备对应于靶序列的转录产物的方法，组合物和试剂盒，通过使用编码一条双链启动子的启动子引物获得环状单链DNA转录底物。本发明对于研究，诊断和治疗应用具有广泛的适用性，例如制备对应于mRNA的cDNA，制备有义或反义探针，检测基因或生物体特异性序列或制备RNAi。

10. 发明申请

WO0071739A9 REVERSE TRANSCRIPTION ACTIVITY FROM BACILLUS STEAROTHERMOPHILUS DNA POLYMERASE IN THE PRESENCE OF MAGNESIUM 审中-公开
标题翻译：来自巴氏杆菌螺旋体DNA聚合酶的逆向转录活性存在于镁
公开(公告)号：WO0071739A9
公开(公告)日：2002-07-04
申请号：PCT/US0013960
申请日：2000-05-19
申请人： EPICT TECHNOLOGIES CORP , SCHANKE JUDITH E T
发明人： SCHANKE JUDITH E T
IPC分类号： C12N15/09 , C12N9/10 , C12N9/12 , C12N15/10 , C12P19/34
CPC分类号： C12N9/1252 , C12N9/1276 , C12N15/1096
摘要： The present invention is directed to a thermostable DNA polymerase from Bacillus stearothermophilus for use in reverse transcription and/or reverse transcriptase-polymerase chain reaction (RT-PCR), where said DNA polymerase shows magnesium ion dependent reverse transcriptase activity and in the substantial absence of manganese ions.
摘要翻译：本发明涉及用于逆转录和/或逆转录酶 - 聚合酶链反应（RT-PCR）的嗜热脂肪芽孢杆菌的热稳定DNA聚合酶，其中所述DNA聚合酶显示镁离子依赖性逆转录酶活性，并且在实质上不存在锰离子。

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