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1. 发明申请

WO2017144620A1 LIGHT-GATED SIGNALING MODULATION 审中-公开
标题翻译：光门控信号调制
公开(公告)号：WO2017144620A1
公开(公告)日：2017-08-31
申请号：PCT/EP2017/054246
申请日：2017-02-23
申请人： MAX PLANCK FLORIDA INSTITUTE FOR NEUROSCIENCE , MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V.
发明人： KWON, Hyungbae , LEE, Dongmin
IPC分类号： C12N15/62 , G01N33/68
CPC分类号： G01N33/5008 , C07K14/035 , C07K14/245 , C07K2319/02 , C07K2319/03 , C07K2319/41 , C07K2319/43 , C07K2319/50 , C12N9/1241 , C12N9/22
摘要： The present invention relates to a nucleic acid molecule encoding a fusion protein, wherein the nucleic acid molecule comprises: (a) a first nucleic acid sequence encoding a transmembrane domain linked to a first biosensor, wherein said first biosensor is a first molecule capable of interacting with a second molecule to form part of a first inducible interaction module, and wherein said first biosensor is linked to the transmembrane domain such that the first biosensor is located intracellularly upon expression of the fusion protein in a cell; (b) a second nucleic acid sequence encoding an effector-activating module, wherein the effector-activating module comprises: (i) a nucleic acid sequence encoding a first part of a protease, wherein said first part of the protease is capable of interacting with a second part of said protease to form an active form of said protease; or (ii) a nucleic acid sequence encoding a second biosensor, wherein said second biosensor is a first molecule capable of interacting with a second molecule to form part of a second inducible interaction module; (c) a third nucleic acid sequence encoding a third biosensor comprising a protease cleavage site, wherein the protease cleavage site is sterically occluded in the absence of a stimulus for said third biosensor and wherein the protease cleavage site becomes accessible in the presence of said stimulus; and (d) a fourth nucleic acid sequence encoding an effector molecule. The present invention further relates to a vector comprising the nucleic acid molecule of the invention, to sets of nucleic acid molecules, to the sets of nucleic acid molecules of the invention comprised in one or more vectors, to a cell expressing a set of nucleic acid molecules according to the invention as well as to a cell comprising the one or more vectors of the invention. Furthermore, the present invention relates to a method for inducing intracellular signaling, as well as to a method for monitoring intracellular signaling.
摘要翻译：本发明涉及编码融合蛋白的核酸分子，其中所述核酸分子包含：（a）编码与第一生物传感器连接的跨膜结构域的第一核酸序列，其中所述第一核酸序列生物传感器是能够与第二分子相互作用以形成第一可诱导相互作用模块的一部分的第一分子，并且其中所述第一生物传感器与跨膜结构域连接，使得第一生物传感器在融合蛋白在细胞中表达时位于细胞内 ; （b）编码效应子活化模块的第二核酸序列，其中所述效应子活化模块包含：（i）编码蛋白酶的第一部分的核酸序列，其中所述蛋白酶的第一部分能够与所述蛋白酶的第二部分以形成所述蛋白酶的活性形式; 或（ii）编码第二生物传感器的核酸序列，其中所述第二生物传感器是能够与第二分子相互作用以形成第二可诱导相互作用模块的一部分的第一分子; （c）编码包含蛋白酶切割位点的第三生物传感器的第三核酸序列，其中所述蛋白酶切割位点在对所述第三生物传感器不存在刺激的情况下被空间阻断，并且其中所述蛋白酶切割位点在所述刺激物 ; 和（d）编码效应分子的第四核酸序列。本发明进一步涉及包含本发明核酸分子，核酸分子集合，包含在一个或多个载体中的本发明核酸分子集合，表达一组核酸分子的细胞的载体根据本发明的分子以及包含本发明的一种或多种载体的细胞。此外，本发明涉及用于诱导细胞内信号传导的方法，以及用于监测细胞内信号传导的方法。

2. 发明申请

WO2017142913A1 METHOD OF MAKING POLYNUCLEOTIDES USING AN ANION TOROIDAL VORTEX 审中-公开
标题翻译：使用阴离子环状涡旋制备多核苷酸的方法
公开(公告)号：WO2017142913A1
公开(公告)日：2017-08-24
申请号：PCT/US2017/017918
申请日：2017-02-15
申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE
发明人： CHURCH, George M. , LEE, Howon , PALLA, Mirko
IPC分类号： C07H21/04 , C07H19/00 , C12P19/30 , C12P19/34 , C12N9/12
CPC分类号： C07H21/04 , C12N9/1241 , C12P19/34
摘要： A method for making a polynucleotide is provided that includes (a) delivering one or more reaction reagents including an error prone or template independent DNA polymerase, cations and a selected nucleotide to a reaction site including an initiator sequence having a terminal nucleotide for a time period and under conditions sufficient to covalently add a desired number of the selected nucleotide to the terminal nucleotide at the 3' end of the initiator such that the selected nucleotide becomes a terminal nucleotide, moving cations away from the initiator sequence using an anion toroidal vortex to inhibit covalent addition of the selected nucleotide by the error prone or template independent DNA polymerase, removing the reaction reagents from the reaction site, and (b) repeating step (a) until the polynucleotide is formed.
摘要翻译：提供了制备多核苷酸的方法，其包括（a）将一种或多种包括易错或模板独立的DNA聚合酶，阳离子和选择的核苷酸的反应试剂递送至包含引发剂序列的反应位点具有末端核苷酸一段时间并在足以将所需数量的所选核苷酸共价添加至引发剂3'末端的末端核苷酸的条件下，使得所选核苷酸成为末端核苷酸，将阳离子移离引发剂使用阴离子环形涡旋序列来抑制通过易错或模板独立的DNA聚合酶共价加入所选核苷酸，从反应位点除去反应试剂，和（b）重复步骤（a）直至形成多核苷酸。 p>

3. 发明申请

WO2017062668A3 DNA VECTORS, TRANSPOSONS AND TRANSPOSASES FOR EUKARYOTIC GENOME MODIFICATION 审中-公开
标题翻译：用于真核基因组修饰的DNA载体，转运蛋白和转运蛋白
公开(公告)号：WO2017062668A3
公开(公告)日：2017-08-03
申请号：PCT/US2016055824
申请日：2016-10-06
申请人： DNA2 0 INC
发明人： MINSHULL JEREMY , WELCH MARK , GOVINDRAJAN SRIDHAR , LEE MAGGIE , CAVES KATE , NESS JON
IPC分类号： C12N15/74 , A61K31/713 , C12N1/19 , C12N15/11 , C12N15/67 , C12N15/85 , C12N15/867 , C12N15/87 , C12N15/90 , C12Q1/68
CPC分类号： C12N9/1241 , C07K2319/09 , C12N15/90 , C12N2800/90 , C12N2830/40 , C12N2840/203 , C12Y207/07
摘要： The present invention provides polynucleotide vectors for high expression of heterologous genes. Some vectors further comprise novel transposons and transposases that further improve expression. Further disclosed are vectors that can be used in a gene transfer system for stably introducing nucleic acids into the DNA of a cell. The gene transfer systems can be used in methods, for example, gene expression, bioprocessing, gene therapy, insertional mutagenesis, or gene discovery.
摘要翻译：本发明提供了用于高表达异源基因的多核苷酸载体。一些载体还包含进一步提高表达的新型转座子和转座酶。进一步公开了可用于基因转移系统中用于将核酸稳定引入细胞DNA中的载体。基因转移系统可用于例如基因表达，生物加工，基因治疗，插入诱变或基因发现的方法中。

4. 发明申请

WO2017124100A1 METHODS FOR EXTENDING THE REPLICATIVE CAPACITY OF SOMATIC CELLS DURING AN EX VIVO CULTIVATION PROCESS 审中-公开
标题翻译：提高体外培养过程中体细胞重复容量的方法
公开(公告)号：WO2017124100A1
公开(公告)日：2017-07-20
申请号：PCT/US2017/013782
申请日：2017-01-17
申请人： MEMPHIS MEATS, INC.
发明人： GENOVESE, Nicholas , DESMET, Danielle Nicole , SCHULZE, Eric
IPC分类号： A23L1/31 , C12N5/077 , C12N15/85
CPC分类号： C12N9/1241 , C12N5/0018 , C12N2510/00
摘要： A product and process for extending the replicative capacity of metazoan somatic cells using targeted genetic amendments to abrogate inhibition of cell-cycle progression during replicative senescence and derive clonal cell lines for scalable applications and industrial production of metazoan cell biomass. An insertion or deletion mutation using guide RNAs targeting the first exon of the transcript encoding each protein is created using CRISPR/Cas9. Targeted amendments result in inactivation of p15 and p16 proteins which increases the proliferative capacity of the modified cell populations relative to their unaltered parental populations. Combining these amendments with ancillary telomerase activity from a genetic construct directing expression of a telomerase protein homolog from a TERT gene, increases the replicative capacity of the modified cell populations indefinitely. One application is to manufacture skeletal muscle for dietary consumption using cells from the poultry species Gallus gallus ; another is from the livestock species Bos taurus.
摘要翻译：使用靶向遗传修饰来延长复制性衰老期间细胞周期进程的抑制并衍生克隆细胞系以用于可扩展应用和工业生产后生动物细胞的用于延长后生动物体细胞的复制能力的产品和方法生物质。使用靶向编码每种蛋白质的转录物的第一外显子的引导RNA的插入或缺失突变使用CRISPR / Cas9产生。靶向修饰导致p15和p16蛋白失活，这增加了修饰的细胞群相对于其未改变的亲本群体的增殖能力。将这些修饰与来自指导来自TERT基因的端粒酶蛋白质同源物表达的基因构建物的辅助端粒末端转移酶活性组合，可无限增加修饰的细胞群的复制能力。一种应用是使用来自家禽物种Gallus gallus的细胞来制造用于饮食消耗的骨骼肌肉; 另一个来自家畜物种金牛座。

5. 发明申请

WO2017103221A1 A GENETICALLY MODIFIED BACTERIAL CELL FACTORY FOR THIAMINE PRODUCTION 审中-公开
标题翻译：一种经遗传修饰的细菌细胞工厂，用于生产亚胺
公开(公告)号：WO2017103221A1
公开(公告)日：2017-06-22
申请号：PCT/EP2016/081598
申请日：2016-12-16
申请人： BIOSYNTIA APS
发明人： GRONENBERG, Luisa , SALOMONSEN, Bo , FERLA, Matteo , GENEE, Hans Jasper
IPC分类号： C12N1/21 , C12N9/06 , C12N9/10 , C12N9/12 , C12N9/16 , C12N9/88 , C12N15/55 , C12P17/16
CPC分类号： C12N9/16 , C12N9/0022 , C12N9/1085 , C12N9/1229 , C12N9/1241 , C12N9/13 , C12N9/88 , C12P17/167 , C12Y301/03
摘要： The invention provides a genetically modified bacterium for production of thiamine; where the bacterium is characterized by a transgene encoding a thiamine monophosphate phosphatase (TMP phosphatase having EC 3.1.3.-) as well as transgenes encoding polypeptides that catalyze steps in the thiamine pathway. The genetically modified bacterium is characterized by enhanced synthesis and release of thiamine into the extracellular environment. The invention further provides a method for producing thiamine using the genetically modified bacterium of the invention; as well as the use of the genetically modified bacterium for extracellular thiamine production.
摘要翻译：本发明提供了用于生产硫胺素的遗传修饰的细菌; 其中细菌的特征在于编码硫胺素单磷酸磷酸酶（具有EC 3.1.3.-的TMP磷酸酶）的转基因以及编码在硫胺素途径中催化步骤的多肽的转基因。该基因修饰的细菌的特征在于增强了硫胺进入细胞外环境的合成和释放。本发明还提供了使用本发明的基因修饰细菌生产硫胺素的方法; 以及使用转基因细菌进行细胞外硫胺素生产。

6. 发明申请

WO2016183403A3 POLYMERASE VARIANTS AND USES THEREOF 审中-公开
标题翻译：聚合物变体及其用途
公开(公告)号：WO2016183403A3
公开(公告)日：2017-01-05
申请号：PCT/US2016032258
申请日：2016-05-13
申请人： GENIA TECH INC
发明人： AYER ARUNA , ARNOLD CLEOMA , SCHWAB CHARLES , THAI EILEEN , LEDERMAN ILYA , MCGAW COLIN , SHULTZ TYLER
IPC分类号： C07K1/00 , C07K14/00 , C07K17/00 , C12N9/12
CPC分类号： C12N9/1241 , C12N9/1252 , C12Y207/07 , C12Y207/07007
摘要： Described herein is a variant pol6 polymerase having at least one mutation selected from H223, N224, Y225, H227, I295, Y342, T343, I357, S360, L361, I363, S365, S366, Y367, P368, D417, E475, Y476, F478, K518, H527, T529, M531, N535, G539, P542, N545, Q546, A547, L549, I550, N552, G553, F558, A596, G603, A610, V615, Y622, C623, D624, I628, Y629, R632, N635, M641, A643, I644, T647, I648, T651, I652, K655, W656, D657, V658, H660, F662, L690 and combinations thereof.
摘要翻译：本文描述的是具有至少一个选自H223，N224，Y225，H227，I295，Y342，T343，I357，S360，L361，I363，S365，S366，Y367，P368，D417，E475，Y476， F478，K518，H527，T529，M531，N535，G539，P542，N545，Q546，A547，L549，I550，N552，G553，F558，A596，G603，A610，V615，Y622，C623，D624，I628，Y629， R632，N635，M641，A643，I644，T647，I648，T651，I652，K655，W656，D657，V658，H660，F662，L690及其组合。

7. 发明申请

WO2016193226A1 METHOD FOR ADDING CAP STRUCTURES TO RNA USING IMMOBILIZED ENZYMES 审中-公开
标题翻译：使用固定化酶将盖结构添加到RNA的方法
公开(公告)号：WO2016193226A1
公开(公告)日：2016-12-08
申请号：PCT/EP2016/062192
申请日：2016-05-30
申请人： CUREVAC AG
发明人： ROOS, Tilmann , YAZDAN PANAH, Benyamin , CONZELMANN, Markus , THESS, Andreas , BUOB, Dominik , KUNZE, Martin , WAGNER, Veronika
IPC分类号： C12N9/12 , C12N9/10 , C12N9/16 , C12N11/02
CPC分类号： C12N9/1241 , C12N9/1007 , C12N9/16 , C12N11/02 , C12Y201/01056 , C12Y201/01057 , C12Y207/0705 , C12Y301/03033
摘要： The present invention relates to an immobilized capping enzyme, preferably an immobilized Vaccinia virus capping enzyme. Furthermore, the present invention relates to an immobilized cap-specific nucleoside 2'-O-methyltransferase, preferably an immobilized Vaccinia virus cap-specific nucleoside 2'-O-methyltransferase. Moreover, the present invention relates to a method for immobilizing said enzymes and to a method of using said enzymes for the addition of a 5'-cap structure to RNAs. Moreover, the present invention relates to an enzyme reactor for performing the capping reaction using said immobilized enzymes and the subsequent separation of the 5'-capped RNA product. In addition, the present invention relates to a kit comprising the capping enzyme and/or the cap-specific nucleoside 2'-O- methyltransferase.
摘要翻译：本发明涉及固定化的封端酶，优选固定化的痘苗病毒覆盖酶。此外，本发明涉及固定化的帽特异性核苷2'-O-甲基转移酶，优选固定化的痘苗病毒帽特异性核苷2'-O-甲基转移酶。此外，本发明涉及一种固定所述酶的方法以及使用所述酶向RNA中添加5'-帽结构的方法。此外，本发明涉及使用所述固定化酶进行封端反应并随后分离5'-封端RNA产物的酶反应器。此外，本发明涉及包含封端酶和/或帽特异性核苷2'-O-甲基转移酶的试剂盒。

8. 发明申请

WO2016168631A1 VECTOR-BASED MUTAGENESIS SYSTEM 审中-公开
标题翻译：基于矢量的MUTAGENESIS系统
公开(公告)号：WO2016168631A1
公开(公告)日：2016-10-20
申请号：PCT/US2016/027795
申请日：2016-04-15
申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE
发明人： BADRAN, Ahmed, Hussein , LIU, David, R.
IPC分类号： C07K14/245 , C07K14/475 , C07K14/575
CPC分类号： C12N15/102 , C12N9/1241 , C12N9/48 , C12N15/1058 , C12N15/70
摘要： Strategies, reagents, methods, and systems for modulating the mutation rate in cells are provided herein. The strategies, reagents, methods, and systems are broadly applicable for the modulation of mutation rates in cells where high mutation rates and/or control over a broad range of mutation rates is desired, for example, in the context of diversifying a nucleic acid sequence or a plurality of such sequences within a population of cells, for the generation of diversified nucleic acid libraries, and for directed evolution of nucleic acids and encoded products.
摘要翻译：本文提供了用于调节细胞突变率的策略，试剂，方法和系统。策略，试剂，方法和系统广泛适用于在需要高范围突变率的高突变率和/或控制的细胞中调节突变率，例如在多样化核酸序列的背景下或细胞群体内的多个这样的序列，用于产生多样化的核酸文库，以及核酸和编码产物的定向进化。

9. 发明申请

WO2016090385A1 SITE-DIRECTED CRISPR/RECOMBINASE COMPOSITIONS AND METHODS OF INTEGRATING TRANSGENES 审中-公开
标题翻译：站点指导的CRISPR /重组体组合物和整合转运体的方法
公开(公告)号：WO2016090385A1
公开(公告)日：2016-06-09
申请号：PCT/US2015/064352
申请日：2015-12-07
申请人： APPLIED STEMCELL, INC.
发明人： TSAI, Ruby, Yanru
IPC分类号： C12N9/22 , C12N15/113 , C12N15/52 , C12N15/85
CPC分类号： C12N15/907 , C07K2319/85 , C12N9/1241 , C12N9/22 , C12N15/11 , C12N2310/20
摘要： Disclosed herein are targeted chimeric polypeptides, compositions thereof, expression vectors, and methods of use thereof, for the generation of transgenic cells, tissues, plants, and animals. The compositions, vectors, and methods of the present invention are also useful in gene therapy and cell therapy techniques. The chimeric polypeptide includes a CRISPR-Cas domain and a recombinase domain.
摘要翻译：本文公开了靶向的嵌合多肽，其组合物，表达载体及其使用方法，用于产生转基因细胞，组织，植物和动物。本发明的组合物，载体和方法也可用于基因治疗和细胞治疗技术。嵌合多肽包括CRISPR-Cas结构域和重组酶结构域。

10. 发明申请

WO2016008602A1 BIOTECHNOLOGISCHE HERSTELLUNG VON LNT, LNNT UND DEREN FUCOSYLIERTEN DERIVATEN 审中-公开
标题翻译：生物技术制造LNT，LNnT的及其衍生物的岩藻糖化
公开(公告)号：WO2016008602A1
公开(公告)日：2016-01-21
申请号：PCT/EP2015/057805
申请日：2015-04-10
申请人： BASF SE
发明人： BAUMGÄRTNER, Florian , SPRENGER, Georg, A. , ALBERMANN, Christoph
IPC分类号： C12N9/10 , A61K31/702
CPC分类号： C12P19/26 , C12N9/1051 , C12N9/1241 , C12N9/90 , C12N15/52 , C12P19/18 , C12Y204/01 , C12Y204/01022 , C12Y204/01062 , C12Y204/01146 , C12Y207/01052 , C12Y207/0703 , C12Y207/07064 , C12Y501/03002
摘要： Die vorliegende Erfindung betrifft primär gentechnisch veränderte Mikroorganismen zur in vivo-Synthese von Lacto-N-tetraose (LNT) bzw. Lacto-N-neotetraose (LNnT) und deren fucosylierten Derivaten, sowie Verwendungen solcher Mikroorganismen in Verfahren zur Herstellung von Lacto-N-tetraose bzw. Lacto-N-neotetraose und deren fucosylierten Derivaten.
摘要翻译：本发明主要涉及一种用于乳-N-四糖（LNT）和乳-N-新四糖（LNnT的）的体内合成遗传修饰的微生物和它们的岩藻糖基衍生物，以及这样的微生物的用途在方法生产乳-N-的四糖或乳-N-新四糖和它们的岩藻糖基衍生物。

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