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1. 发明申请

WO2004078349A1 A METHOD OF PREPARING MULTI-MODAL ANION-EXCHANGE LIGANDS 审中-公开
标题翻译：一种制备多模式阴离子交换配体的方法
公开(公告)号：WO2004078349A1
公开(公告)日：2004-09-16
申请号：PCT/SE2004/000317
申请日：2004-03-05
申请人： AMERSHAM BIOSCIENCES AB , CARLSSON, Ulrika , MALOISEL, Jean-Luc , THEVENIN, Nicolas
发明人： CARLSSON, Ulrika , MALOISEL, Jean-Luc , THEVENIN, Nicolas
IPC分类号： B01J41/04
CPC分类号： B01J20/3242 , B01D15/363 , B01J20/289 , B01J20/3219 , B01J20/3285 , B01J41/12 , B01J41/20
摘要： The invention relates to a method of preparing multi-modal anion-exchange ligands, which comprises providing a cyclic three-functional scaffold comprising an amine, a carbonyl and a thiol; optionally derivatisation of the amine of the scaffold to provide an anion-exchanging group and/or to protect the amine; and aminolysis to open up the derivative by adding a reagent comprising an amine coupled to a residue R in order to add said amine to the carbonyl carbon of the opened scaffold. The scaffold is advantageously homocysteine thiolactone. In one embodiment, the method comprises an additional step of immobilising the opened scaffold to a base matrix to provide a separation medium, such as a chromatography medium.
摘要翻译：本发明涉及一种制备多模式阴离子交换配体的方法，其包括提供包含胺，羰基和硫醇的环状三官能支架; 任选地衍生化支架的胺以提供阴离子交换基团和/或保护胺; 并通过加入包含偶联到残基R的胺的试剂以将所述胺加入到开放的支架的羰基碳中，开启衍生物。支架有利的是同型半胱氨酸硫内酯。在一个实施方案中，该方法包括将开放的支架固定在基础基质上以提供分离介质如色谱介质的附加步骤。

2. 发明申请

WO2005082926A1 ANTIBODY PURIFICATION 审中-公开
标题翻译：抗体纯化
公开(公告)号：WO2005082926A1
公开(公告)日：2005-09-09
申请号：PCT/SE2005/000292
申请日：2005-02-24
申请人： AMERSHAM BIOSCIENCES AB , JOHANSSON, Bo-Lennart , JOHANSSON, Hans, J. , LJUNGLÖF, Anders , MALOISEL, Jean-Luc , THEVENIN, Nicolas
发明人： JOHANSSON, Bo-Lennart , JOHANSSON, Hans, J. , LJUNGLÖF, Anders , MALOISEL, Jean-Luc , THEVENIN, Nicolas
IPC分类号： C07K1/16
CPC分类号： C07K1/18 , B01D15/362 , B01D15/3809 , B01D15/3847 , B01J39/07 , B01J39/26 , B01J47/014 , C07K1/165 , B01D15/327
摘要： The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.
摘要翻译：本发明涉及一种从溶液中的污染物中分离抗体的方法，该方法包括使该溶液与包含固定有多元配体的载体的色谱树脂接触，其中多模式配体包含至少一个阳离子交换基团和至少一个芳族或杂芳族环系统。在一个实施方案中，芳族或非芳族实体的成环原子选自C，S或O，并且阳离子交换基团是弱阳离子交换剂。本方法可以用作单步骤程序或作为在蛋白A柱上捕获后的抛光步骤。

3. 发明申请

WO2004111646A1 NOVEL MS REAGENTS 审中-公开
公开(公告)号：WO2004111646A1
公开(公告)日：2004-12-23
申请号：PCT/SE2004/000860
申请日：2004-06-03
申请人： AMERSHAM BIOSCIENCES AB , JAMES, Peter , MALOISEL, Jean-Luc , CARLSSON, Ulrika , BUSSON, Philippe , WÅHLANDER, Åsa
发明人： JAMES, Peter , MALOISEL, Jean-Luc , CARLSSON, Ulrika , BUSSON, Philippe , WÅHLANDER, Åsa
IPC分类号： G01N33/532
CPC分类号： G01N33/6848 , C07K1/13
摘要： The present invention relates to novel MS reagents comprising the following formula: CG-LG1-BG-LG2-RG Wherein CG is Charged Group, LG1 is Linker Group 1, BG is Bridge Group, LG2 is Linker Group 2, RG is Reactive Group, And CG, LG1, BG, LG2, and RG are covalently connected. Preferably the reagent molecule fully or partly contains a label/labels, which is possible to detect, by subsequent analysis such as mass spectroscopy. The invention is to be used for identification and quantification of proteins/ peptides by MS and specifically via the analysis of parent ions. By using two differently labelled reagent molecules the invention enables, for example, relative quantification of protein/peptide in a sample. In a second aspect the invention relates to a kit comprising the reagent molecules. The invention also relates to use of the reagent molecules for analysis of biomolecules.
摘要翻译：本发明涉及包含下式的新型MS试剂：CG-LG1-BG-LG2-RG其中CG为带电基团，LG1为连接基团1，BG为桥组，LG2为连接基团2，RG为活性基团， CG，LG1，BG，LG2和RG共同连接。优选地，试剂分子完全或部分地含有可以通过后续分析如质谱法检测的标签/标记。本发明用于通过MS鉴定和定量蛋白质/肽，特别是通过母体离子的分析。通过使用两种不同标记的试剂分子，本发明能够例如样品中蛋白质/肽的相对定量。在第二方面，本发明涉及包含试剂分子的试剂盒。本发明还涉及试剂分子用于分析生物分子的用途。

4. 发明申请

WO2004078311A1 A METHOD OF PREPARING LIGANDS FOR HYDROPHOBIC INTERACTION CHROMATOGRAPHY 审中-公开
标题翻译：制备疏水相互作用色谱的配体的方法
公开(公告)号：WO2004078311A1
公开(公告)日：2004-09-16
申请号：PCT/SE2004/000316
申请日：2004-03-05
申请人： AMERSHAM BIOSCIENCES AB , JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc , THEVENIN, Nicolas
发明人： JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc , THEVENIN, Nicolas
IPC分类号： B01D15/08
CPC分类号： B01J20/3285 , B01D15/325 , B01D15/327 , B01J20/286
摘要： The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.
摘要翻译：本发明是制备用于疏水相互作用色谱（HIC）的多峰配体的方法，其包括提供包含硫醇，胺和羰基的环状支架; 用试剂衍生氮以引入主要相互作用; 和所得衍生物的氨解，由此在羰基旁边引入二次相互作用; 其中所述主要相互作用和所述第二相互作用中的至少一个包含疏水基团，并且其中所述相互作用中的非相互作用包括带电基团，即离子交换配体。本发明还包括一种分离介质，其包含固定在基础基质上的这种多峰配体。

5. 发明申请

WO2004078310A1 A METHOD OF PREPARING AFFINITY LIGANDS 审中-公开
标题翻译：制备亲属配偶的方法
公开(公告)号：WO2004078310A1
公开(公告)日：2004-09-16
申请号：PCT/SE2004/000315
申请日：2004-03-05
申请人： AMERSHAM BIOSCIENCES AB , MALOISEL, Jean-Luc , THEVENIN, Nicolas
发明人： MALOISEL, Jean-Luc , THEVENIN, Nicolas
IPC分类号： B01D15/08
CPC分类号： B01J20/286 , B01D15/3804 , B01J20/321 , B01J20/3212 , B01J20/3219 , B01J20/3251 , B01J20/3255 , B01J20/3259
摘要： The present invention is a method of preparing affinity ligands, which method comprises to provide a cyclic scaffold comprising a thiol group, a carbonyl group and an amine group; to derivatise the amine group of said scaffold with an electrophile that carries either a functionality capable of affinity interaction or a functionality capable of a second interaction with a target molecule; and to open up the derivatised scaffold and add an amine that carries either a functionality capable of a second interaction or a functionality capable of affinity interaction with a target molecule. The method provides provide affinity ligands, which are capable of affinity interaction as well as a second kind of interaction.
摘要翻译：本发明是制备亲和配体的方法，该方法包括提供包含硫醇基，羰基和胺基的环状支架; 用携带亲和力相互作用的官能团或能够与靶分子进行第二次相互作用的官能团的亲电子试剂衍生所述支架的胺基团; 并打开衍生的支架并加入携带能够进行第二相互作用的官能团或能够与靶分子亲和相互作用的官能团的胺。该方法提供了提供亲和力配体，其能够进行亲和力相互作用以及第二种相互作用。

6. 发明申请

WO2005082483A1 A PROCESS FOR THE PURIFICATION OF ANTIBODIES 审中-公开
标题翻译：抗体的纯化方法
公开(公告)号：WO2005082483A1
公开(公告)日：2005-09-09
申请号：PCT/SE2005/000293
申请日：2005-02-25
申请人： AMERSHAM BIOSCIENCES AB , GRÖNBERG, Anna , JOHANSSON, Bo-Lennart , JOHANSSON, Hans, J. , MALOISEL, Jean-Luc , THEVENIN, Nicolas
发明人： GRÖNBERG, Anna , JOHANSSON, Bo-Lennart , JOHANSSON, Hans, J. , MALOISEL, Jean-Luc , THEVENIN, Nicolas
IPC分类号： B01D15/08
CPC分类号： B01J20/3253 , B01D15/3847 , B01J20/3242 , B01J20/3246 , B01J20/3255 , B01J20/3285
摘要： The present invention relates to a process for the purification of antibodies from one or more impurities in a liquid, which process comprises contacting said liquid with a first chromatography resin comprised of a support to which multi-modal ligands have been immobilised to adsorb the antibodies to the resin, wherein each multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system; adding an eluent to release the antibodies from the resin; and contacting the eluate so obtained with a second chromatography resin. In one embodiment, the ring-forming atoms of the aromatic or heteroaromatic entity are selected from the group con-sisting of C, S and O, and the cation exchanging group is a weak cation exchanger.
摘要翻译：本发明涉及一种用于从液体中的一种或多种杂质纯化抗体的方法，该方法包括使所述液体与由已经固定有多模式配体的载体组成的第一层析树脂接触以将抗体吸附到所述树脂，其中每个多模式配体包含至少一个阳离子交换基团和至少一个芳族或杂芳族环系统; 加入洗脱液从树脂中释放出抗体; 并将如此获得的洗脱液与第二层析树脂接触。在一个实施方案中，芳族或杂芳族实体的成环原子选自C，S和O所组成的组，阳离子交换基团是弱阳离子交换剂。

7. 发明申请

WO2003024588A1 GENERATION OF ION EXCHANGER MEDIA 审中-公开
标题翻译：离子交换介质的生成
公开(公告)号：WO2003024588A1
公开(公告)日：2003-03-27
申请号：PCT/SE2002/001650
申请日：2002-09-12
申请人： AMERSHAM BIOSCIENCES AB , MALOISEL, Jean-Luc , THEVENIN, Nicolas
发明人： MALOISEL, Jean-Luc , THEVENIN, Nicolas
IPC分类号： B01J20/22
CPC分类号： B01J20/3212 , B01J20/3204 , B01J20/321 , B01J20/3219 , B01J39/17 , B01J39/18 , B01J39/26
摘要： The present invention relates to a method of generating a separation medium comprising mixed mode cation-exchanger ligands coupled to a base matrix, which method comprises to provide a scaffold comprising a functional group and exhibiting a cyclic core structure; derivatise the scaffold with a reagent comprising a reactive group coupled to a residue R by reacting the functional group of the scaffold with said reactive group; open the cyclic structure of the resulting derivative; and react the product with a base matrix comprising a re-active group. The scaffold presents at least two functionalities; one sulphur-comprising group for coupling to the base matrix and one group that can be transformed into an ionic group.
摘要翻译：本发明涉及一种产生分离介质的方法，该分离介质包含与基础基质偶联的混合模式阳离子交换配体，该方法包括提供包含官能团并显示环状核心结构的支架; 通过使支架的官能团与所述反应性基团反应，用包含与残基R偶联的反应性基团的试剂衍生支架; 打开所得衍生物的环状结构; 并使产物与包含重活性基团的基质基质反应。脚手架至少有两个功能; 一个用于与基础基质偶联的含硫基团和一个可以转化成离子基团的基团。

8. 发明申请

WO2002095419A2 PEPTIDE ANALYSIS USING A SOLID SUPPORT 审中-公开
标题翻译：使用固体支持的肽分析
公开(公告)号：WO2002095419A2
公开(公告)日：2002-11-28
申请号：PCT/US2002/016247
申请日：2002-05-20
申请人： AMERSHAM BIOSCIENCES AB , THE PROCTER AND GAMBLE COMPANY BECKETT RIDGE TECHNICAL CENTER , BHIKHABHAI, Ramagauri , LIMINGA, Maria , MALOISEL, Jean-Luc , PALMGREN, Ronnie , KEOUGH, Thomas, W. , YOUNGQUIST, Robert, S. , VAUGHN, Harold, L. , YELM, Ken, E.
发明人： BHIKHABHAI, Ramagauri , LIMINGA, Maria , MALOISEL, Jean-Luc , PALMGREN, Ronnie , KEOUGH, Thomas, W. , YOUNGQUIST, Robert, S. , VAUGHN, Harold, L. , YELM, Ken, E.
IPC分类号： G01N33/68
CPC分类号： G01N33/6848 , G01N33/58 , G01N33/6824 , G01N33/6851
摘要： The present invention relates to a method of identifying a polypeptide, which method comprises the steps of (a) derivatization of the N-terminus of the polypeptide, or the N-termini of one or more peptides of the polypeptide, with at least one acidic reagent which comprises a sulfonyl moiety coupled to an activated acid moiety to provide one or more peptide derivatives; analyzing at least one such derivative using a mass spectrometric technique to provide a fragmentation pattern, and (c) interpreting the fragmentation pattern obtained, wherein the peptide or polypeptide is immobilized to a solid support at least during step(a). Furthermore, the present invention also relates to a kit for identifying a polypeptide by a mass spectrometric technique.
摘要翻译：本发明涉及一种鉴定多肽的方法，该方法包括以下步骤：（a）使多肽的一个或多个肽的N-末端的N末端或至少一个酸性的试剂，其包含与活化的酸部分偶联以提供一种或多种肽衍生物的磺酰基部分; 使用质谱技术分析至少一种这样的衍生物以提供分裂模式，和（c）解释获得的断裂模式，其中至少在步骤（a）期间将肽或多肽固定在固体支持物上。此外，本发明还涉及通过质谱技术鉴定多肽的试剂盒。

9. 发明申请

WO2004076475A1 A METHOD OF GENERATING METAL CHELATING AFFINITY LIGANDS 审中-公开
标题翻译：一种生成金属螯合亲和力配体的方法
公开(公告)号：WO2004076475A1
公开(公告)日：2004-09-10
申请号：PCT/SE2003/001903
申请日：2003-12-08
申请人： AMERSHAM BIOSCIENCES AB , ANDERSSON, Lars, C. , AXÉN, Andreas , GEBRU, Tesfai , MALOISEL, Jean-Luc , TEDEBARK, Ulf
发明人： ANDERSSON, Lars, C. , AXÉN, Andreas , GEBRU, Tesfai , MALOISEL, Jean-Luc , TEDEBARK, Ulf
IPC分类号： C07K1/22
CPC分类号： B01J45/00 , B01D15/3828 , B01J20/286 , B01J20/3251 , B01J20/3265 , C07C323/58 , C07D333/36
摘要： The present invention relates to a method of generating polydentate metal chelating affinity ligands, which method comprises the steps of providing a cyclic scaffold comprising a carbonyl, an adjacent sulphur and a nucleophile; providing a polydentate metal chelating affinity ligand arm on each scaffold by derivatisation of said nucleophile; ring-opening of the scaffold by adding a reagent that adds more metal chelating affinity ligand arm(s) to the scaffold; and, if required, deprotecting the functionalities of the ligand arm(s) so provided. In the most preferred embodiment of the method, the ring-opening and the deprotection steps are performed in a single step.
摘要翻译：本发明涉及产生多齿金属螯合亲和配体的方法，该方法包括提供包含羰基，相邻硫和亲核体的环状支架的步骤; 通过所述亲核试剂的衍生化在每个支架上提供多齿金属螯合亲和配体臂; 通过添加向支架中添加更多金属螯合亲和配体臂的试剂来开环支架; 并且如果需要，使如此提供的配体臂的功能脱保护。在该方法的最优选实施方案中，开环和脱保护步骤在单步中进行。

10. 发明申请

WO2003051484A1 SEPARATION METHOD 审中-公开
标题翻译：分离方法
公开(公告)号：WO2003051484A1
公开(公告)日：2003-06-26
申请号：PCT/SE2002/002350
申请日：2002-12-17
申请人： AMERSHAM BIOSCIENCES AB , JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc
发明人： MALOISEL, Jean-Luc
IPC分类号： B01D15/08
CPC分类号： B01J20/262 , B01D15/368 , B01D15/3847 , B01J20/265 , B01J20/285 , B01J20/3204 , B01J20/321 , B01J20/3212 , B01J20/3219 , B01J20/3246 , B01J20/3248 , B01J20/3253 , B01J20/3255 , C07K1/16
摘要： The present invention relates to a method of separating a compound from a liquid, which method comprises providing a separation matrix comprising at least one uncharged ligand; providing a liquid wherein the compound to be separated is present in a positively charged state; contacting said matrix with said liquid to adsorb the compound; and removing the liquid. The uncharged ligands possess a quadrupole or dipole moment, allowing for a cation-p interaction between the compound and the ligand. The present invention also encompasses the use of a separation matrix, which comprises an uncharged group that possesses a quadrupole or dipole moment, in said method.
摘要翻译：本发明涉及从液体中分离化合物的方法，该方法包括提供包含至少一种不带电配位体的分离基质; 提供其中待分离的化合物以带正电的状态存在的液体; 使所述基质与所述液体接触以吸附化合物; 并除去液体。不带电荷的配体具有四极或偶极矩，允许化合物和配体之间的阳离子-P相互作用。本发明还包括在所述方法中使用分离基质，其包含具有四极或偶极矩的不带电荷的基团。

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