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    • 10. 发明申请
    • NEW CHROMATOGRAPHIC MEDIA BASED ON PHENOXY ALKYL AND ALKOXY-OR PHENOXY-PHENYL ALKYL LIGANDS
    • 基于苯氧基和烷基或苯基 - 苯基氨基酸的新色谱介质
    • WO2010059550A1
    • 2010-05-27
    • PCT/US2009/064536
    • 2009-11-16
    • MALLINCKRODT BAKER, INC.DEORKAR, NanduTHIYAGARAJAN, B.
    • DEORKAR, NanduTHIYAGARAJAN, B.
    • B01J20/287B01J20/32B01D15/32G01N30/02
    • B01J20/3253B01J20/287B01J20/3227B01J20/3242B01J20/3246B01J2220/54G01N30/02B01D15/426B01D15/325
    • A reverse phase chromatographic media selected from media of the formula: [X-C 6 H 4 -(O) m -(CH 2 ) n ] q -Z and hydrophobic end-capped media of said formula, wherein n is a numeral of from 1 to 4, and m is 0 or 1, and when m is 1 X is selected from the group H, an alkyl group having from 1 to 6 carbon atoms and a phenyl group, and when m is 0 then X is selected from an alkoxy group having from 1 to 6 carbon atoms and a phenoxy group, Z is the backbone of a silica or hydrophilic polymer chromatographic support, and q is a number equal to the number of ligands attached to the backbone of the silica or hydrophobic polymer chromatographic support, with the proviso that when said reverse phase chromatographic media of the formula is not end-capped with hydrophobic groups X is not H when m =1. These novel chromatographic media are prepared by reacting: (a) a chromatographic media support selected from (1) a silica support having hydroxyl groups on the surface of the silica backbone or (2) a hydrophilic polymer support having hydroxyl, amine or imine groups on the surface of the polymer backbone, with (b) a reactant of the formula [X-C 6 H 4 -(O) m -(CH 2 ) n ] p -Si (Y) 4-p wherein p is a numeral of from 1 to 3, Y is a chloro or alkoxy group having from 1 to 4 carbon atoms in the alkoxy group, and m, n and X are as defined above, and optionally end-capping the resulting media by reacting it with a hydrophobic end-capping reactant.. The resulting chromatographic media with these ligands attached to the backbone of the silica or hydrophilic polymer support provides chromatographic media that offers analyte separation capability in the aqueous mobile phase.
    • 选自下式的介质的反相色谱介质:[X-C6H4-(O)m-(CH2)n] qZ和所述式的疏水封端的介质,其中n为1至4的数字,以及 m为0或1,当m为1时,选自H,具有1至6个碳原子的烷基和苯基,当m为0时,X选自具有1个 至6个碳原子和苯氧基,Z是二氧化硅或亲水性聚合物色谱载体的主链,q是等于与二氧化硅或疏水性聚合物色谱载体的主链连接的配体数目,数目等于 当式的所述反相色谱介质未用疏水性基团封端时,当m = 1时,X不为H。 这些新型色谱介质通过以下反应制备:(a)色谱介质载体,其选自(1)在二氧化硅骨架的表面上具有羟基的二氧化硅载体,或(2)具有羟基,胺或亚胺基团的亲水性聚合物载体, 聚合物主链的表面与(b)式[X-C6H4-(O)m-(CH2)n] p-Si(Y)4-p的反应物,其中p是1至3的数字 ,Y是在烷氧基中具有1至4个碳原子的氯或烷氧基,m,n和X如上所定义,并且任选地通过使其与疏水性封端反应物反应来封端所得介质。 所得到的色谱介质与这些配体连接在二氧化硅或亲水聚合物支架的主链上,提供色谱介质,在水性流动相中提供分析物分离能力。