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1. 发明申请

WO2005042131A1 NOVEL SEPARATION MATRIX 审中-公开
标题翻译：新的分离矩阵
公开(公告)号：WO2005042131A1
公开(公告)日：2005-05-12
申请号：PCT/SE2004/001583
申请日：2004-11-01
申请人： AMERSHAM BIOSCIENCES AB , BERGSTRÖM, Jan , GLAD, Gunnar , JOHANSSON, Bo-Lennart , LJUNGLÖF, Anders , MALOISEL, Jean-Luc
发明人： BERGSTRÖM, Jan , GLAD, Gunnar , JOHANSSON, Bo-Lennart , LJUNGLÖF, Anders , MALOISEL, Jean-Luc
IPC分类号： B01D15/08
CPC分类号： B01J20/3285 , B01D15/364 , B01J20/286 , B01J20/3244
摘要： The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.
摘要翻译：本发明涉及由耦合到多孔载体的表面的配体（例如一种或多种多孔颗粒）组成的分离基质，其中配体在载体内提供至少一种化学梯度。在最有利的实施方案中，化学梯度是配体密度梯度。本发明还涉及一种提供包含耦合到多孔载体表面的配体的分离基质的方法，其中至少一种配体密度梯度由至少一种试剂的溶剂控制扩散提供到多孔载体中。

2. 发明申请

WO2006126942A1 REGENERATION OF A CHROMATOGRAPHY MATRIX 审中-公开
标题翻译：色谱矩阵的再生
公开(公告)号：WO2006126942A1
公开(公告)日：2006-11-30
申请号：PCT/SE2006/000599
申请日：2006-05-22
申请人： GE HEALTHCARE BIO-SCIENCES AB , JOHANSSON, Hans, J. , LJUNGLÖF, Anders , ÅBERG, Per-Mikael
发明人： JOHANSSON, Hans, J. , LJUNGLÖF, Anders , ÅBERG, Per-Mikael
IPC分类号： B01J20/34 , B01D15/08 , C07K1/22
CPC分类号： C07K1/22 , B01D15/203
摘要： The present invention relates to a process of regenerating a separation matrix, such as a chromatography matrix, comprising adsorption of at least one target molecule by contacting a mobile phase comprising at target molecule(s) with a matrix; removal of unbound material by washing the matrix; elution of target molecule(s) by contacting the matrix with an eluent; reducing regeneration by contacting said matrix with a reducing agent; alkaline regeneration by contacting the matrix with an alkaline solution; and equilibration of the matrix.
摘要翻译：本发明涉及再生分离基质如色谱基质的方法，其包括通过使包含靶分子的流动相与基质接触来吸附至少一种靶分子; 通过洗涤基质去除未结合的材料; 通过使基质与洗脱液接触来洗脱目标分子; 通过使所述基质与还原剂接触来减少再生; 通过使基质与碱性溶液接触进行碱性再生; 并平衡基质。

3. 发明申请

WO2010080065A1 AFFINITY CHROMATOGRAPHY MATRIX 审中-公开
标题翻译：亲属色谱矩阵
公开(公告)号：WO2010080065A1
公开(公告)日：2010-07-15
申请号：PCT/SE2010/050016
申请日：2010-01-11
申请人： GE HEALTHCARE BIO-SCIENCES AB , JOHANSSON, Hans, J. , LJUNGLÖF, Anders , PALMGREN, Ronnie
发明人： JOHANSSON, Hans, J. , LJUNGLÖF, Anders , PALMGREN, Ronnie
IPC分类号： C07K1/22 , B01D15/38 , C12N15/00 , C07K14/31 , C07K16/00
CPC分类号： C07K16/065 , B01D15/3809 , B01J20/286 , B01J20/3244 , B01J20/3248 , B01J20/3274
摘要： The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilised to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the adsorbed immunoglobulin containing proteins; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods being that each of the ligands consists essentially of a monomer or dimer SpA or protein Z or a functional variant thereof.
摘要翻译：本发明涉及从液体中分离一种或多种含免疫球蛋白的蛋白质的方法。该方法包括首先使液体与包含固定在载体上的配体的分离基质接触; 允许含有免疫球蛋白的蛋白质通过与配体的相互作用吸附到基质上; 随后是洗涤吸附的含有蛋白质的免疫球蛋白的任选步骤; 并通过使基质与释放蛋白质的洗脱液接触来回收所述含有免疫球蛋白的蛋白质。该方法改进了先前的分离方法，即每种配体基本上由单体或二聚体SpA或蛋白质Z或其功能变体组成。

4. 发明申请

WO2005094960A1 A METHOD FOR CHROMATOGRAPHIC PURIFICATION 审中-公开
标题翻译：一种用于色谱纯化的方法
公开(公告)号：WO2005094960A1
公开(公告)日：2005-10-13
申请号：PCT/SE2005/000467
申请日：2005-03-31
申请人： AMERSHAM BIOSCIENCES AB , VAN ALSTINE, James , HOUSHMAND, Hamid , LJUNGLÖF, Anders , ÅBERG, Per-Mikael
发明人： VAN ALSTINE, James , HOUSHMAND, Hamid , LJUNGLÖF, Anders , ÅBERG, Per-Mikael
IPC分类号： B01D15/08
CPC分类号： B01D15/3804 , B01D15/1864 , B01D15/327 , B01D15/361 , B01D15/426 , C07K1/22 , G01N30/461
摘要： The present invention relates to a method of isolating a target compound from other components of a liquid, which method comprises at least two chromatographic steps, in any sequence of order, wherein the mobile phase is contacted with an affinity chromatography matrix and/or an ion-exchange chromatography matrix and/or a hydrophobic interaction chromatography matrix, wherein the contacting with at least one of the matrices takes place in the presence of at least one non-ionic polyether; and obtaining the target compound(s) in a separate fraction from the last chromatographic step. In the most preferred embodiment, the non-ionic polyether is poly(ethylene glycol) (PEG).
摘要翻译：本发明涉及一种将目标化合物与液体的其它组分分离的方法，该方法包括至少两个按顺序的色谱步骤，其中流动相与亲和层析基质和/或离子 - 交换层析基质和/或疏水相互作用层析基质，其中与至少一种基质的接触在至少一种非离子聚醚存在下进行; 并从最后一个色谱步骤中分离得到目标化合物。在最优选的实施方案中，非离子聚醚是聚（乙二醇）（PEG）。

5. 发明申请

WO2005082926A1 ANTIBODY PURIFICATION 审中-公开
标题翻译：抗体纯化
公开(公告)号：WO2005082926A1
公开(公告)日：2005-09-09
申请号：PCT/SE2005/000292
申请日：2005-02-24
申请人： AMERSHAM BIOSCIENCES AB , JOHANSSON, Bo-Lennart , JOHANSSON, Hans, J. , LJUNGLÖF, Anders , MALOISEL, Jean-Luc , THEVENIN, Nicolas
发明人： JOHANSSON, Bo-Lennart , JOHANSSON, Hans, J. , LJUNGLÖF, Anders , MALOISEL, Jean-Luc , THEVENIN, Nicolas
IPC分类号： C07K1/16
CPC分类号： C07K1/18 , B01D15/362 , B01D15/3809 , B01D15/3847 , B01J39/07 , B01J39/26 , B01J47/014 , C07K1/165 , B01D15/327
摘要： The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.
摘要翻译：本发明涉及一种从溶液中的污染物中分离抗体的方法，该方法包括使该溶液与包含固定有多元配体的载体的色谱树脂接触，其中多模式配体包含至少一个阳离子交换基团和至少一个芳族或杂芳族环系统。在一个实施方案中，芳族或非芳族实体的成环原子选自C，S或O，并且阳离子交换基团是弱阳离子交换剂。本方法可以用作单步骤程序或作为在蛋白A柱上捕获后的抛光步骤。

6. 发明申请

WO2005073712A1 METHOD AND SYSTEM FOR REDUCING TOTAL SAMPLE COMPLEXITY 审中-公开
标题翻译：减少总样本复杂度的方法和系统
公开(公告)号：WO2005073712A1
公开(公告)日：2005-08-11
申请号：PCT/SE2005/000085
申请日：2005-01-26
申请人： AMERSHAM BIOSCIENCES AB , AXELMAN, Johan , LJUNGLÖF, Anders
发明人： AXELMAN, Johan , LJUNGLÖF, Anders
IPC分类号： G01N30/72
CPC分类号： G01N30/7233 , G01N30/02 , G01N30/461 , G01N30/463 , Y10T436/24 , Y10T436/255 , B01D15/362 , B01D15/325 , B01D15/363
摘要： A method for reducing total sample complexity in native or digested biological sample(s), before analysis thereof by mass spectrometry, comprising the following steps: a) selecting a fraction from the entire native or digested biological sample(s) on the basis of pI-value, said fraction comprising native or digested sample representing a subset of or the entire substance population in the sample, said fraction being obtained by e.g. anion exchange chromatography, isoelectric focussing or chromatofocussing; b) separating native or digested sample substances from each other, wherein said separation is by cation exchange chromatography; and c) analysing aid substances by mass spectrometry. The invention also relates to a system for reducing total sample complexity in the above method, comprising a high capacity charge-selective column (anion exchange, isoelectric focussing or chromatofocussing) coupled to a MDLC work flow path comprising a cation exchange column and a RPC column. The system is followed by a MS/MS instrument.
摘要翻译：一种在通过质谱分析之前降低天然或消化的生物样品中的总样本复杂性的方法，包括以下步骤：a）基于pI从整个天然或消化的生物样品中选择一个级分所述级分包含表示样品中的子集或整个物质群的天然或消化样品，所述级分通过例如阴离子交换层析，等电聚焦或色谱聚焦; b）将天然或消化的样品物质彼此分离，其中所述分离是通过阳离子交换色谱法; 和c）通过质谱分析辅助物质。本发明还涉及一种用于降低上述方法中的总样品复杂度的系统，其包括耦合到包含阳离子交换柱和RPC柱的MDLC工作流路的高容量电荷选择性柱（阴离子交换，等电聚焦或色谱聚焦）。该系统后面是MS / MS仪器。

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