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1. 发明申请

WO2004111646A1 NOVEL MS REAGENTS 审中-公开
公开(公告)号：WO2004111646A1
公开(公告)日：2004-12-23
申请号：PCT/SE2004/000860
申请日：2004-06-03
申请人： AMERSHAM BIOSCIENCES AB , JAMES, Peter , MALOISEL, Jean-Luc , CARLSSON, Ulrika , BUSSON, Philippe , WÅHLANDER, Åsa
发明人： JAMES, Peter , MALOISEL, Jean-Luc , CARLSSON, Ulrika , BUSSON, Philippe , WÅHLANDER, Åsa
IPC分类号： G01N33/532
CPC分类号： G01N33/6848 , C07K1/13
摘要： The present invention relates to novel MS reagents comprising the following formula: CG-LG1-BG-LG2-RG Wherein CG is Charged Group, LG1 is Linker Group 1, BG is Bridge Group, LG2 is Linker Group 2, RG is Reactive Group, And CG, LG1, BG, LG2, and RG are covalently connected. Preferably the reagent molecule fully or partly contains a label/labels, which is possible to detect, by subsequent analysis such as mass spectroscopy. The invention is to be used for identification and quantification of proteins/ peptides by MS and specifically via the analysis of parent ions. By using two differently labelled reagent molecules the invention enables, for example, relative quantification of protein/peptide in a sample. In a second aspect the invention relates to a kit comprising the reagent molecules. The invention also relates to use of the reagent molecules for analysis of biomolecules.
摘要翻译：本发明涉及包含下式的新型MS试剂：CG-LG1-BG-LG2-RG其中CG为带电基团，LG1为连接基团1，BG为桥组，LG2为连接基团2，RG为活性基团， CG，LG1，BG，LG2和RG共同连接。优选地，试剂分子完全或部分地含有可以通过后续分析如质谱法检测的标签/标记。本发明用于通过MS鉴定和定量蛋白质/肽，特别是通过母体离子的分析。通过使用两种不同标记的试剂分子，本发明能够例如样品中蛋白质/肽的相对定量。在第二方面，本发明涉及包含试剂分子的试剂盒。本发明还涉及试剂分子用于分析生物分子的用途。

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