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1. 发明申请

WO2005082926A1 ANTIBODY PURIFICATION 审中-公开
标题翻译：抗体纯化
公开(公告)号：WO2005082926A1
公开(公告)日：2005-09-09
申请号：PCT/SE2005/000292
申请日：2005-02-24
申请人： AMERSHAM BIOSCIENCES AB , JOHANSSON, Bo-Lennart , JOHANSSON, Hans, J. , LJUNGLÖF, Anders , MALOISEL, Jean-Luc , THEVENIN, Nicolas
发明人： JOHANSSON, Bo-Lennart , JOHANSSON, Hans, J. , LJUNGLÖF, Anders , MALOISEL, Jean-Luc , THEVENIN, Nicolas
IPC分类号： C07K1/16
CPC分类号： C07K1/18 , B01D15/362 , B01D15/3809 , B01D15/3847 , B01J39/07 , B01J39/26 , B01J47/014 , C07K1/165 , B01D15/327
摘要： The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.
摘要翻译：本发明涉及一种从溶液中的污染物中分离抗体的方法，该方法包括使该溶液与包含固定有多元配体的载体的色谱树脂接触，其中多模式配体包含至少一个阳离子交换基团和至少一个芳族或杂芳族环系统。在一个实施方案中，芳族或非芳族实体的成环原子选自C，S或O，并且阳离子交换基团是弱阳离子交换剂。本方法可以用作单步骤程序或作为在蛋白A柱上捕获后的抛光步骤。

2. 发明申请

WO2004078311A1 A METHOD OF PREPARING LIGANDS FOR HYDROPHOBIC INTERACTION CHROMATOGRAPHY 审中-公开
标题翻译：制备疏水相互作用色谱的配体的方法
公开(公告)号：WO2004078311A1
公开(公告)日：2004-09-16
申请号：PCT/SE2004/000316
申请日：2004-03-05
申请人： AMERSHAM BIOSCIENCES AB , JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc , THEVENIN, Nicolas
发明人： JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc , THEVENIN, Nicolas
IPC分类号： B01D15/08
CPC分类号： B01J20/3285 , B01D15/325 , B01D15/327 , B01J20/286
摘要： The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.
摘要翻译：本发明是制备用于疏水相互作用色谱（HIC）的多峰配体的方法，其包括提供包含硫醇，胺和羰基的环状支架; 用试剂衍生氮以引入主要相互作用; 和所得衍生物的氨解，由此在羰基旁边引入二次相互作用; 其中所述主要相互作用和所述第二相互作用中的至少一个包含疏水基团，并且其中所述相互作用中的非相互作用包括带电基团，即离子交换配体。本发明还包括一种分离介质，其包含固定在基础基质上的这种多峰配体。

3. 发明申请

WO2005082483A1 A PROCESS FOR THE PURIFICATION OF ANTIBODIES 审中-公开
标题翻译：抗体的纯化方法
公开(公告)号：WO2005082483A1
公开(公告)日：2005-09-09
申请号：PCT/SE2005/000293
申请日：2005-02-25
申请人： AMERSHAM BIOSCIENCES AB , GRÖNBERG, Anna , JOHANSSON, Bo-Lennart , JOHANSSON, Hans, J. , MALOISEL, Jean-Luc , THEVENIN, Nicolas
发明人： GRÖNBERG, Anna , JOHANSSON, Bo-Lennart , JOHANSSON, Hans, J. , MALOISEL, Jean-Luc , THEVENIN, Nicolas
IPC分类号： B01D15/08
CPC分类号： B01J20/3253 , B01D15/3847 , B01J20/3242 , B01J20/3246 , B01J20/3255 , B01J20/3285
摘要： The present invention relates to a process for the purification of antibodies from one or more impurities in a liquid, which process comprises contacting said liquid with a first chromatography resin comprised of a support to which multi-modal ligands have been immobilised to adsorb the antibodies to the resin, wherein each multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system; adding an eluent to release the antibodies from the resin; and contacting the eluate so obtained with a second chromatography resin. In one embodiment, the ring-forming atoms of the aromatic or heteroaromatic entity are selected from the group con-sisting of C, S and O, and the cation exchanging group is a weak cation exchanger.
摘要翻译：本发明涉及一种用于从液体中的一种或多种杂质纯化抗体的方法，该方法包括使所述液体与由已经固定有多模式配体的载体组成的第一层析树脂接触以将抗体吸附到所述树脂，其中每个多模式配体包含至少一个阳离子交换基团和至少一个芳族或杂芳族环系统; 加入洗脱液从树脂中释放出抗体; 并将如此获得的洗脱液与第二层析树脂接触。在一个实施方案中，芳族或杂芳族实体的成环原子选自C，S和O所组成的组，阳离子交换基团是弱阳离子交换剂。

4. 发明申请

WO2006001771A1 SEPARATION MATRIX AND METHOD OF PURIFICATION 审中-公开
标题翻译：分离矩阵和纯化方法
公开(公告)号：WO2006001771A1
公开(公告)日：2006-01-05
申请号：PCT/SE2005/001002
申请日：2005-06-22
申请人： AMERSHAM BIOSCIENCES AB , MALOISEL, Jean-Luc , JOHANSSON, Bo-Lennart , GLAD, Gunnar , NORRMAN, Nils
发明人： MALOISEL, Jean-Luc , JOHANSSON, Bo-Lennart , GLAD, Gunnar , NORRMAN, Nils
IPC分类号： G01N30/48
CPC分类号： B01J20/3212 , B01D15/426 , B01J20/286 , B01J20/289 , B01J20/3219 , B01J20/3251
摘要： The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilised, wherein said ligands comprise at least one sulphonamide and the R group of the sulphonyl comprises an aromatic group. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to the separation matrix.
摘要翻译：本发明涉及由已经固定有配体的多孔载体组成的分离基质，其中所述配体包含至少一个磺酰胺，并且磺酰基的R基包括芳族基团。磺酰胺的氮可以是仲胺或叔胺。本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到分离基质上分离免疫球蛋白样化合物的方法。

5. 发明申请

WO2005061543A1 PURIFICATION OF IMMUNOGLOBULINS 审中-公开
标题翻译：免疫球蛋白的纯化
公开(公告)号：WO2005061543A1
公开(公告)日：2005-07-07
申请号：PCT/SE2004/002007
申请日：2004-12-21
申请人： AMERSHAM BIOSCIENCES AB , GLAD, Gunnar , JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc , NORRMAN, Nils
发明人： GLAD, Gunnar , JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc , NORRMAN, Nils
IPC分类号： C07K16/06
CPC分类号： B01J20/262 , B01J20/267 , B01J20/28019 , B01J20/28023 , B01J20/28033 , B01J20/28042 , B01J20/286 , B01J20/289 , B01J20/3092 , B01J20/3208 , B01J20/3212 , B01J20/3219 , B01J20/3246 , B01J20/3248 , B01J20/3251 , B01J20/3255 , B01J20/3272 , B01J20/3285 , B01J2220/54 , B01J2220/58 , C07K16/065 , Y10T428/249963
摘要： The present invention relates to a separation matrix comprised of a porous support to which ligands have been immobilised, wherein said ligands comprise at least one aliphatic sulphonamide. The nitrogen of the sulphonamide may be a secondary or tertiary amine. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulin-like compounds by adsorption to a separation matrix that comprises aliphatic sulphonamide ligands.
摘要翻译：本发明涉及由已经固定有配体的多孔载体组成的分离基质，其中所述配体包含至少一种脂族磺酰胺。磺酰胺的氮可以是仲胺或叔胺。本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到包含脂族磺酰胺配体的分离基质上分离免疫球蛋白样化合物的方法。

6. 发明申请

WO2005042131A1 NOVEL SEPARATION MATRIX 审中-公开
标题翻译：新的分离矩阵
公开(公告)号：WO2005042131A1
公开(公告)日：2005-05-12
申请号：PCT/SE2004/001583
申请日：2004-11-01
申请人： AMERSHAM BIOSCIENCES AB , BERGSTRÖM, Jan , GLAD, Gunnar , JOHANSSON, Bo-Lennart , LJUNGLÖF, Anders , MALOISEL, Jean-Luc
发明人： BERGSTRÖM, Jan , GLAD, Gunnar , JOHANSSON, Bo-Lennart , LJUNGLÖF, Anders , MALOISEL, Jean-Luc
IPC分类号： B01D15/08
CPC分类号： B01J20/3285 , B01D15/364 , B01J20/286 , B01J20/3244
摘要： The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.
摘要翻译：本发明涉及由耦合到多孔载体的表面的配体（例如一种或多种多孔颗粒）组成的分离基质，其中配体在载体内提供至少一种化学梯度。在最有利的实施方案中，化学梯度是配体密度梯度。本发明还涉及一种提供包含耦合到多孔载体表面的配体的分离基质的方法，其中至少一种配体密度梯度由至少一种试剂的溶剂控制扩散提供到多孔载体中。

7. 发明申请

WO2007064281A1 HYDROPHOBIC INTERACTION CHROMATOGRAPHY 审中-公开
标题翻译：疏水相互作用色谱
公开(公告)号：WO2007064281A1
公开(公告)日：2007-06-07
申请号：PCT/SE2006/001369
申请日：2006-11-30
申请人： GE HEALTHCARE BIO-SCIENCES AB , GLAD, Gunnar , JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc , NORRMAN, Nils
发明人： GLAD, Gunnar , JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc , NORRMAN, Nils
IPC分类号： B01D15/16 , B01D15/32 , C07K16/06
CPC分类号： C07K16/065 , B01D15/327 , B01J20/262 , B01J20/265 , B01J20/285 , B01J20/286 , B01J20/289
摘要： The present invention relates to a method of isolating target compounds from a liquid, which method comprises providing a mobile phase, which comprises at least one target compound and wherein the conductivity corresponds to 0.6 M; contacting said mobile phase with a separation matrix comprising one or more sulphonamide groups to adsorb one or more target compounds; contacting an eluent with said matrix to release target compound(s), wherein the conductivity of the eluent is reduced as compared to the mobile phase conductivity and the pH is substantially equivalent to the mobile phase pH; and, optionally, recovering at least one target compound or a purified liquid.
摘要翻译：本发明涉及一种从液体中分离目标化合物的方法，该方法包括提供流动相，其包含至少一种目标化合物，其中电导率对应于0.6M; 使所述流动相与包含一个或多个磺酰胺基团的分离基质接触以吸附一种或多种目标化合物; 使洗脱液与所述基质接触以释放目标化合物，其中与流动相电导率相比，洗脱液的电导率降低，并且pH基本上等于流动相pH; 和任选地回收至少一种目标化合物或纯化液体。

8. 发明申请

WO2006043896A1 CHROMATOGRAPHY LIGAND 审中-公开
标题翻译：色谱仪
公开(公告)号：WO2006043896A1
公开(公告)日：2006-04-27
申请号：PCT/SE2005/001592
申请日：2005-10-21
申请人： GE HEALTHCARE BIO-SCIENCES AB , ENGSTRAND, Carina , FORSS, Annika , GLAD, Gunnar , JOHANSSON, Bo-Lennart , JOHANSSON, Hans, J. , MALOISEL, Jean-Luc
发明人： ENGSTRAND, Carina , FORSS, Annika , GLAD, Gunnar , JOHANSSON, Bo-Lennart , JOHANSSON, Hans, J. , MALOISEL, Jean-Luc
IPC分类号： B01J41/04 , B01D15/32 , B01D15/36 , C07K16/00
CPC分类号： B01D15/3828 , B01D15/327 , B01D15/363 , B01D15/3804 , B01D15/3809 , B01D15/3847 , B01J20/286 , B01J20/3253 , B01J20/3285 , B01J41/20 , C07K1/165 , C07K1/36 , C07K16/00 , C07K16/065
摘要： The present invention relates to a chromatography ligand defined by the following formula (I) R 1 -R 2 -N(R 3 )-R 4 -R 5 wherein R 1 is a substituted or non-substituted phenyl group; R 2 is a hydrocarbon chain comprising 0-4 carbon atoms; R 3 is a hydrocarbon chain comprising 1-3 carbon atoms; R 4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R 5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.
摘要翻译：本发明涉及由下式（I）定义的色谱配体R 1 -R 2 -N（R 3 N）-R 其中R 1是取代或未取代的苯基;其中R 1是取代或未取代的苯基; R 2是包含0-4个碳原子的烃链; R 3是包含1-3个碳原子的烃链; R 4是包含1-5个碳原子的烃链; 并且R 5是OH或H.本发明还包括分离基质，其包含与多孔载体如颗粒或膜偶联的所述配体。根据本发明的配体和基质可用于纯化生物分子或有机化合物，例如蛋白质，多肽，DNA等。根据本发明的有利用途是抗体的纯化。

9. 发明申请

WO2012134381A1 METHOD AND MEANS FOR SAMPLE PREPARATION 审中-公开
标题翻译：样品制备方法与手段
公开(公告)号：WO2012134381A1
公开(公告)日：2012-10-04
申请号：PCT/SE2012/050342
申请日：2012-03-29
申请人： GE HEALTHCARE BIO-SCIENCES AB , GLAD, Gunnar , JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc
发明人： GLAD, Gunnar , JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc
IPC分类号： G01N30/50 , B01D15/20 , B01D15/34
CPC分类号： G01N1/405 , B01D15/34 , B01D15/3866 , B01D15/3885 , B01J20/28009 , B01J20/285 , B01J20/3204 , B01J20/3227 , B01J20/3274 , B01J20/3293 , G01N30/14 , G01N2030/143
摘要： The present invention relates to a method for depletion of undesired molecules and/or enrichment of desired molecules from a sample comprising high abundant as well as low abundant molecules, comprising the following steps: a) providing a separation material comprising a solid phase (beads) comprising an inner porous core material comprising magnetic particles and an outer porous shell with a porosity equal or denser than that of the shell; b) adding the sample to the separation material; c) adsorbing a first fraction of molecules with a molecular weight of 500-50000 Da in the core and simultaneously excluding a second fraction of molecules from binding to the core and the shell, wherein the molecular weight of the second fraction molecules is at least 5 preferably 10 times higher than the molecular weight of the first fraction and d) eluting the desired molecules from the separation material, wherein step d) and optionally step c)is performed using an oscillating power/field applied over the separation material. The first fraction of molecules are for example drugs with a mw of about 700 Da, small proteins/peptides with an mw of about 7000 Da or proteins with a mw of about 40000 Da.
摘要翻译：本发明涉及从包含高丰度和低丰度分子的样品中消耗不想要的分子和/或富集所需分子的方法，包括以下步骤：a）提供包含固相（珠）包括包含磁性颗粒的内部多孔芯材料和具有等于或比密封件的孔隙度更高的孔隙的外部多孔壳体; b）将样品加入到分离材料中; c）在芯中吸附分子量为500-50000Da的第一级分子，同时排除第二级分子与核心和壳结合，其中第二级分子的分子量至少为5 优选比第一级分分子量高10倍，和d）从分离材料洗脱所需的分子，其中步骤d）和任选的步骤c）使用施加在分离材料上的振荡功率/场进行。分子的第一部分例如是具有约700Da的mw的药物，具有约7000Da的mw的小蛋白质/肽或约40000Da的蛋白质。

10. 发明申请

WO2010005364A1 SEPARATION MEDIUM FOR CHROMATOGRAPHY OF VARIOUS BIOMOLECULES 审中-公开
标题翻译：分离介质用于各种生物分子的色谱分析
公开(公告)号：WO2010005364A1
公开(公告)日：2010-01-14
申请号：PCT/SE2009/050666
申请日：2009-06-04
申请人： GE HEALTHCARE BIO-SCIENCES AB , GLAD, Gunnar , JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc , NORRMAN, Nils
发明人： GLAD, Gunnar , JOHANSSON, Bo-Lennart , MALOISEL, Jean-Luc , NORRMAN, Nils
IPC分类号： C07K1/22 , B01D15/36 , B01J20/285 , C07K16/00
CPC分类号： B01D15/3828 , B01D15/166 , B01D15/36 , B01J20/28092 , B01J20/285 , B01J20/286 , B01J20/3242 , B01J20/3293 , C07K1/16 , C07K1/22 , C07K16/00
摘要： The present invention relates to a separation medium, comprising an inner core of a porous material provided with charged ligands, and an outer lid comprising a porous material provided with charged ligands, wherein the charge of the ligands in the inner core is opposite that of the charge of the ligands in the lid. The present invention also relates to a method for biomolecule separation comprising applying a sample to the above separation medium, wherein large molecules are prevented from entering the medium by charge repulsion from the medium and small molecules are captured in the inner core.
摘要翻译：本发明涉及一种分离介质，其包括具有带电配位体的多孔材料的内核和包含带有带电配位体的多孔材料的外盖，其中内芯中配体的电荷与盖中配体的电荷。本发明还涉及一种用于生物分子分离的方法，包括将样品施加到上述分离介质中，其中通过从介质的电荷排斥来防止大分子进入介质，并且小分子被捕获在内核中。

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