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1. 发明申请

WO2011003020A1 METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION 审中-公开
标题翻译：用于核酸扩增的方法和组合物
公开(公告)号：WO2011003020A1
公开(公告)日：2011-01-06
申请号：PCT/US2010/040819
申请日：2010-07-01
申请人： GEN-PROBE INCORPORATED , BRENTANO, Steven T. , LYAKHOV, Dmitry , CARLSON, James D. , BECKER, Michael M. , NELSON, Norman C. , ARNOLD, JR., Lyle J.
发明人： BRENTANO, Steven T. , LYAKHOV, Dmitry , CARLSON, James D. , BECKER, Michael M. , NELSON, Norman C. , ARNOLD, JR., Lyle J.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6865 , C12Q1/6844 , C12Q1/6848 , C12Q1/6853 , C12Q2549/119 , C12Q2537/143 , C12Q2531/143
摘要： Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.
摘要翻译：用于进行扩增反应的组合物，反应混合物和方法，包括多重扩增反应，其中所述方法包括使用包含连接的第一和第二扩增寡聚体成员的一种或多种扩增寡聚体复合物。一方面，将扩增寡聚物复合物与靶核酸杂交，然后捕获具有杂交扩增寡聚物复合物的靶核酸，并洗涤其它组分。靶核酸的靶序列被预扩增以产生第一扩增产物。第一扩增产物在一个或多个二级扩增反应中扩增以产生第二扩增产物。

2. 发明申请

WO2008080029A2 METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION 审中-公开
公开(公告)号：WO2008080029A2
公开(公告)日：2008-07-03
申请号：PCT/US2007/088473
申请日：2007-12-20
申请人： GEN-PROBE INCORPORATED , BRENTANO, Steven T. , CARLSON, James D. , LYAKHOV, Dmitry , NELSON, Norman C.
发明人： BRENTANO, Steven T. , CARLSON, James D. , LYAKHOV, Dmitry , NELSON, Norman C.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6881 , C12Q1/6834 , C12Q1/6844 , C12Q1/6865 , C12Q2525/155 , C12Q2525/186 , C12Q2525/197 , C12Q2531/143 , C12Q2565/543
摘要： Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

3. 发明申请

WO2005087951A3 REAGENTS, METHODS AND KITS FOR USE IN DEACTIVATING NUCLEIC ACIDS 审中-公开
标题翻译：用于消除核酸的试剂，方法和试剂盒
公开(公告)号：WO2005087951A3
公开(公告)日：2005-12-01
申请号：PCT/US2005007153
申请日：2005-03-04
申请人： GEN PROBE INC , NELSON NORMAN C , BROWNE KENNETH A , DAI LIZHONG , RUSSELL JAMES , FILIPOWSKY MARK E , KAMINSKY MARGARITA B , KACIAN DANIEL L
发明人： NELSON NORMAN C , BROWNE KENNETH A , DAI LIZHONG , RUSSELL JAMES , FILIPOWSKY MARK E , KAMINSKY MARGARITA B , KACIAN DANIEL L
IPC分类号： A61K47/00 , A61L2/16 , A61L2/18 , C11D3/395 , C12Q1/68
CPC分类号： C12Q1/6802 , A61L2/186 , C11D3/3956 , C12Q1/6848
摘要： The present invention relates to reagents for use in deactivating nucleic acids and methods of making and using the same.
摘要翻译：本发明涉及用于失活核酸的试剂及其制备和使用方法。

4. 发明申请

WO2014153260A1 METHODS FOR AMPLIFICATION OF NUCLEIC ACIDS ON SOLID SUPPORT 审中-公开
标题翻译：在固体支持物上放大核酸的方法
公开(公告)号：WO2014153260A1
公开(公告)日：2014-09-25
申请号：PCT/US2014/029817
申请日：2014-03-14
申请人： ARNOLD, Lyle, J. , NELSON, Norman, C.
发明人： ARNOLD, Lyle, J. , NELSON, Norman, C.
IPC分类号： C12Q1/68 , C12P19/34 , C12N9/10
CPC分类号： C12P19/34 , C12Q1/6853 , C12Q1/6865 , C12Q2525/186 , C12Q2533/101 , C12Q2565/515 , C12Q2565/537 , C12Q2531/143
摘要： The present invention provides methods for amplifying a nucleic acid from a mixture of nucleic acids utilizing a solid support. Methods are provided utilizing user-defined primer oligonucleotides for directional amplification. Methods are also provided utilizing blocker and displacer oligonucleotides for generating amplified target nucleic acids of defined length. A sample is applied to the solid support and the target nucleic acid within the sample binds a first oligonucleotide affixed to a solid support. A primer sequence containing a target binding region and a polymerase promoter sequence is annealed to the bound target nucleic acid and extended producing a first duplex nucleic acid. The target sequence is removed leaving a first nucleic acid that binds a second oligonucleotide affixed to a solid support. The second oligonucleotide is extended to produce a second duplex nucleic acid that contains a second nucleic acid. The second nucleic acid is amplified by adding a polymerase.
摘要翻译：本发明提供了利用固体支持物从核酸混合物中扩增核酸的方法。使用用于定向扩增的用户定义的引物寡核苷酸提供方法。还提供了利用用于产生具有一定长度的扩增靶核酸的阻断剂和置换寡核苷酸的方法。将样品施加到固体支持物上，并且样品内的靶核酸结合固定在固体支持物上的第一寡核苷酸。包含靶结合区和聚合酶启动子序列的引物序列与结合的靶核酸退火并延长产生第一双链体核酸。去除靶序列，留下结合固定支持物上的第二寡核苷酸的第一核酸。扩展第二寡核苷酸以产生含有第二核酸的第二双链体核酸。通过加入聚合酶扩增第二个核酸。

5. 发明申请

WO2014145138A2 METHODS FOR AMPLIFICATION OF NUCLEIC ACIDS UTILIZING CLAMP OLIGONULEOTIDES 审中-公开
标题翻译：使用钳夹OLIGOULEOTIDES的核酸增殖方法
公开(公告)号：WO2014145138A2
公开(公告)日：2014-09-18
申请号：PCT/US2014/029850
申请日：2014-03-14
申请人： ARNOLD, Lyle, J. , NELSON, Norman, C.
发明人： ARNOLD, Lyle, J. , NELSON, Norman, C.
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q2521/501 , C12Q2525/307 , C12Q2549/126
摘要： The present invention provides methods of amplifying a target nucleic acid utilizing a clamp oligonucleotide comprising a first target-binding region on the 3 '-terminus and a second target-binding region on the 5 '-terminus and tether region in between. The tether region may comprise a variety of user-defined sequences or elements that allow for further manipulation of the target nucleic acid. Such as, for example, capture followed by amplification, identification and/or sequencing. The target-binding regions bind to the target nucleic acid, the 3'-terminus functions as a primer to initiate extension across the target nucleic acid sequence and ligation of the gap results in formation of a circularized nucleic acid. This circular template can be used in a variety of processes, including amplification and sequencing.
摘要翻译：本发明提供利用包含3'末端上的第一靶结合区和其间5'末端和系链区之间的第二靶结合区的钳位寡核苷酸扩增靶核酸的方法。系链区域可以包含允许进一步操纵靶核酸的各种用户定义的序列或元件。例如，进行捕获，随后进行扩增，鉴定和/或测序。靶结合区域与靶核酸结合，3'-末端用作引物以引发靶核酸序列的延伸，并且间隙的连接导致形成环化的核酸。该圆形模板可用于各种过程，包括扩增和测序。

6. 发明申请

WO2009002447A1 INSTRUMENT AND RECEPTACLES FOR USE IN PERFORMING PROCESSES 审中-公开
标题翻译：用于执行过程的仪器和插座
公开(公告)号：WO2009002447A1
公开(公告)日：2008-12-31
申请号：PCT/US2008/007685
申请日：2008-06-20
申请人： GEN-PROBE INCORPORATED , BREIDENTHAL, Scott S. , FAN, Sara H. , KACIAN, Daniel L. , LEE, Richard S. , NELSON, Norman C. , SCOTT, Matthew J. , TAYLOR, Jason A.
发明人： BREIDENTHAL, Scott S. , FAN, Sara H. , KACIAN, Daniel L. , LEE, Richard S. , NELSON, Norman C. , SCOTT, Matthew J. , TAYLOR, Jason A.
IPC分类号： B01L3/00 , G01N35/00 , B01F11/00
CPC分类号： B01F11/00 , B01F3/1271 , B01F11/0045 , B01F11/0071 , B01F13/0059 , B01F15/00214 , B01F2215/0037 , B01L3/502 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/0621 , B01L2200/0647 , B01L2200/0673 , B01L2200/0689 , B01L2200/10 , B01L2200/14 , B01L2200/16 , B01L2300/0816 , B01L2300/087 , B01L2300/0887 , B01L2300/123 , B01L2300/1805 , B01L2300/1822 , B01L2300/1883 , B01L2400/0457 , B01L2400/0478 , B01L2400/0481 , B01L2400/0655 , B01L2400/0677 , C12N15/1006 , C12Q1/6806 , C12Q1/6844 , G01N1/44 , G01N21/01 , G01N21/6428 , G01N21/645 , G01N35/0098 , G01N2021/6419 , G01N2021/6421 , G01N2021/6432 , G01N2021/6439 , G01N2021/6463 , G01N2035/00544 , G01N2201/0627 , Y10T137/0391 , Y10T436/143333 , Y10T436/25 , Y10T436/255
摘要： A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle.
摘要翻译：具有多个互连室的容器，其布置成允许独立地或同时地执行多个工艺步骤或工艺。制造容器以将液体与干燥试剂分开并保持干燥试剂的稳定性。包括不混溶的液体，如油，以控制加工材料的加载，促进干燥试剂的混合和重构，限制蒸发，控制反应材料的加热，浓缩固体支持材料以防止流体连接堵塞，为流体转移，并防止工艺材料粘附到室表面。插座可以适用于具有处理器具的系统，该处理器具包括用于选择性地移动腔室和检测器之间的流体物质的致动器系统。致动器系统可以被布置成集中存在于样品中的分析物。检测器可用于检测由容器的内容物发射的光信号。

7. 发明申请

WO2014145128A3 METHODS FOR AMPLIFICATION OF NUCLEIC ACIDS UTILIZING A CIRCULARIZED TEMPLATE PREPARED FROM A TARGET NUCLEIC ACID 审中-公开
标题翻译：利用目标核酸制备的循环模板扩增核酸的方法
公开(公告)号：WO2014145128A3
公开(公告)日：2014-11-20
申请号：PCT/US2014029829
申请日：2014-03-14
申请人： ARNOLD LYLE J , NELSON NORMAN C
发明人： ARNOLD LYLE J , NELSON NORMAN C
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q2531/119 , C12Q2531/125 , C12Q2537/162
摘要： The present invention provides methods of amplifying a target nucleic acid utilizing a circularized template. Circularization may be achieved utilizing a bridging oligonucleotide or an inverter primer. The bridging oligonucleotide or inverted primer is extended forming a concatemeric amplicon that can then be used as a template to provide exponential amplification of the target nucleic acid.
摘要翻译：本发明提供了利用环化模板扩增靶核酸的方法。循环可以利用桥接寡核苷酸或逆转录引物来实现。桥接寡核苷酸或反向引物延伸形成连接的扩增子，然后可以用作模板以提供靶核酸的指数扩增。

8. 发明申请

WO2014145176A1 METHODS FOR AMPLIFYING FRAGMENTED TARGET NUCLEIC ACIDS UTILIZING AN ASSEMBLER SEQUENCE 审中-公开
标题翻译：使用组装序列扩增目标核酸的方法
公开(公告)号：WO2014145176A1
公开(公告)日：2014-09-18
申请号：PCT/US2014/029893
申请日：2014-03-15
申请人： ARNOLD, Lyle, J. , NELSON, Norman, C.
发明人： ARNOLD, Lyle, J. , NELSON, Norman, C.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/686 , C12Q2525/204 , C12Q2537/162
摘要： The present invention provides methods of amplifying a fragmented target nucleic acid containing short target nucleic acid fragments utilizing an assembler sequence to convert these short fragments into longer sequences enabling their identification and interrogation. This is particularly important when attempting to identify small genetic variations, such as SNVs, present in highly fragmented nucleic acid samples. Amplification is accomplished by hybridizing the short target nucleic acid sequences to the assembler sequence, where these short sequences serve as primers for extension. Since the fragmented target nucleic acids that contain SNVs are utilized as primers on the assembler sequence they are preserved during amplification and can be detected.
摘要翻译：本发明提供了利用汇编序列扩增含有短靶核酸片段的片段化靶核酸的方法，以将这些短片段转化成更长的序列，使其能够进行鉴定和询问。当试图鉴定存在于高度片段化的核酸样品中的小的遗传变异（例如SNV）时，这尤其重要。通过将短目标核酸序列杂交到汇编序列来完成扩增，其中这些短序列用作扩增的引物。由于含有SNV的片段化靶核酸作为汇编序列上的引物，因此在扩增过程中被保留并可以被检测。

9. 发明申请

WO2014145128A2 METHODS FOR AMPLIFICATION OF NUCLEIC ACIDS UTILIZING A CIRCULARIZED TEMPLATE PREPARED FROM A TARGET NUCLEIC ACID 审中-公开
标题翻译：使用从目标核酸制备的圆形模板来放大核酸的方法
公开(公告)号：WO2014145128A2
公开(公告)日：2014-09-18
申请号：PCT/US2014/029829
申请日：2014-03-14
申请人： ARNOLD, Lyle, J. , NELSON, Norman, C.
发明人： ARNOLD, Lyle, J. , NELSON, Norman, C.
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q2531/119 , C12Q2531/125 , C12Q2537/162
摘要： The present invention provides methods of amplifying a target nucleic acid utilizing a circularized template. Circularization may be achieved utilizing a bridging oligonucleotide or an inverter primer. The bridging oligonucleotide or inverted primer is extended forming a concatemeric amplicon that can then be used as a template to provide exponential amplification of the target nucleic acid.
摘要翻译：本发明提供利用环化模板扩增靶核酸的方法。可以使用桥连寡核苷酸或反向引物实现环化。桥接寡核苷酸或反向引物延伸形成一个可以用作模板以提供靶核酸的指数扩增的并联扩增子。

10. 发明申请

WO2010088496A1 SYSTEMS AND METHODS FOR DETECTING A SIGNAL AND APPLYING THERMAL ENERGY TO A SIGNAL TRANSMISSION ELEMENT 审中-公开
标题翻译：用于检测信号并将热能应用于信号传输元件的系统和方法
公开(公告)号：WO2010088496A1
公开(公告)日：2010-08-05
申请号：PCT/US2010/022548
申请日：2010-01-29
申请人： GEN-PROBE INCORPORATED , OPALSKY, David , WALKER, George T. , NELSON, Norman C. , LEE, Richard S. , FAN, Sara H.
发明人： OPALSKY, David , WALKER, George T. , NELSON, Norman C. , LEE, Richard S. , FAN, Sara H.
IPC分类号： B01L7/00 , G01N21/00 , G01N35/00
CPC分类号： G01N21/03 , B01L3/5027 , B01L3/505 , B01L7/52 , B01L9/527 , B01L2200/04 , B01L2200/146 , B01L2300/021 , B01L2300/044 , B01L2300/0654 , B01L2300/0672 , B01L2300/0816 , B01L2300/087 , B01L2300/1827 , B01L2400/043 , B01L2400/0478 , B01L2400/0481 , B01L2400/0487 , B01L2400/0605 , B01L2400/0666 , G01N21/0332 , G01N2021/0328 , G01N2021/0346 , G01N2035/00346
摘要： A signal detection system configured for detecting a signal emitted by the contents of a reaction receptacle is also configured to apply thermal energy to a portion of the reaction receptacle to affect a reaction occurring within the reaction receptacle. More particularly, a system for detecting electromagnetic radiation emitted by the contents of a reaction receptacle includes a transmission element configured for transmitting electromagnetic radiation from the contents of the receptacle, a thermal element associated with the transmission element and configured to apply thermal energy to at least a portion of the receptacle, and a detector configured to receive electromagnetic radiation from the transmission element and to generate a signal corresponding to a characteristic of the electromagnetic radiation received by the detector.
摘要翻译：被配置为检测由反应容器的内容物发射的信号的信号检测系统还被配置为将热能施加到反应容器的一部分以影响在反应容器内发生的反应。更具体地，用于检测由反应容器的内容物发射的电磁辐射的系统包括传输元件，其被配置为从容器的内容物传输电磁辐射，与传输元件相关联的热元件，并且被配置为将热能施加到至少插座的一部分，以及被配置为从传输元件接收电磁辐射并产生对应于由检测器接收的电磁辐射的特性的信号的检测器。

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