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1. 发明申请

WO2017015348A1 SEQUENCE-INDEPENDENT NUCLEIC ACID ASSEMBLY 审中-公开
标题翻译：序列独立核酸组装
公开(公告)号：WO2017015348A1
公开(公告)日：2017-01-26
申请号：PCT/US2016/043104
申请日：2016-07-20
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人： COLEMAN, Todd, Prentice , KYRIAKAKIS, Phillip
IPC分类号： C40B50/14 , C40B50/18 , C12Q1/68 , C07K1/00
CPC分类号： C40B50/14 , B01J19/0046 , B01J2219/00605 , B01J2219/00722 , B01J2219/00729 , C12Q1/68 , C12Q1/6862 , C40B50/18 , C12Q2565/515
摘要： Disclosed is a method for assembling nucleic acid fragments in a sequence-independent way. The method entails attaching a nucleic acid fragment to a solid phase, and contacting the first nucleic acid fragment with a second nucleic acid fragment such that the second nucleic acid fragment is ligated to the first nucleic acid fragment in a directional way. These steps can be repeated multiple times to assemble additional nucleic acid fragments. The method can further include a step of recovering the ligated nucleic acid to obtain the assembled nucleic acid.
摘要翻译：公开了以序列无关的方式组装核酸片段的方法。该方法需要将核酸片段连接到固相，并使第一核酸片段与第二核酸片段接触，使得第二核酸片段以有向的方式连接到第一核酸片段。这些步骤可以重复多次以组装另外的核酸片段。该方法还可以包括回收连接的核酸以获得组装的核酸的步骤。

2. 发明申请

WO2014009007A1 MICROARRAY-BASED METHODS FOR IDENTIFYING SINGLE NUCLEOTIDES AT SPECIFIC POSITIONS IN NUCLEIC ACIDS 审中-公开
标题翻译：用于识别核酸中特定位置的单核细胞的基于微阵列的方法
公开(公告)号：WO2014009007A1
公开(公告)日：2014-01-16
申请号：PCT/EP2013/002021
申请日：2013-07-09
申请人： OAKLABS GMBH
发明人： SCHAD, Martina , KALLARACKAL, Jim
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6827 , C12Q1/6837 , C12Q1/6846 , C12Q1/6858 , C12Q2521/131 , C12Q2525/131 , C12Q2525/155 , C12Q2535/125 , C12Q2565/501 , C12Q2565/515
摘要： The present invention relates to methods for identifying single nucleotides at specific positions in at least one target nucleic acid. The methods involve the synthesis of a plurality of different primers using a first DNA microarray, the template-dependent extension of the primers by a single detectably labeled nucleotide, the hybridization of the labeled and extended primers to oligonucleotides on a second DNA microarray, and the determination of the nucleotide to be identified by detecting the detectable label. The methods of the present invention are suitable for determining the genotype of an organism at a single nucleotide polymorphic site, assessing the genetic similarity between organisms or populations of organisms, identifying at least one single nucleotide polymorphic site as a diagnostic disease marker, and diagnosing a disease in a subject.
摘要翻译：本发明涉及鉴定至少一种靶核酸中特定位置的单个核苷酸的方法。所述方法包括使用第一DNA微阵列合成多种不同的引物，通过单个可检测标记的核苷酸引物的模板依赖性延伸，将标记的和延伸的引物与第二DNA微阵列上的寡核苷酸杂交，以及通过检测可检测标记确定待鉴定的核苷酸。本发明的方法适用于确定单个核苷酸多态性位点处生物体的基因型，评估生物体或生物群体之间的遗传相似性，鉴定至少一个单核苷酸多态性位点作为诊断性疾病标志物，并诊断疾病在一个科目。

3. 发明申请

WO2010106109A1 MULTI-USE OF BIOCHIPS 审中-公开
标题翻译：生物多样性的多用途
公开(公告)号：WO2010106109A1
公开(公告)日：2010-09-23
申请号：PCT/EP2010/053476
申请日：2010-03-17
申请人： FEBIT HOLDING GMBH , STÄHLER, Peer, F. , STÄHLER, Cord, F. , BEIER, Markus , PETERMANN, Rabea , GÜIMIL GARCIA, Ramon
发明人： STÄHLER, Peer, F. , STÄHLER, Cord, F. , BEIER, Markus , PETERMANN, Rabea , GÜIMIL GARCIA, Ramon
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q2565/515 , C12Q2537/161
摘要： The present invention relates to methods for regenerating a nucleic acid array by applying regeneration conditions comprising e.g. a competitive de-hybridisation step (e.g. using nucleotides or oligonucleotides), heat, low stringency conditions (e.g. low salt conditions, organic solvents such as formamide and ethanol or destabilising compounds such as urea), addition of helicase enzymes and/or degradation of target molecules (e.g. using alkaline pH or nucleases). The target molecules in the first and further samples may be differently indexed, e.g. by carrying different labels.
摘要翻译：本发明涉及通过应用再生条件来再生核酸阵列的方法，竞争性去杂交步骤（例如使用核苷酸或寡核苷酸），热，低严格条件（例如低盐条件，有机溶剂如甲酰胺和乙醇或去稳定化合物如尿素），添加解旋酶和/或靶标降解分子（例如使用碱性pH或核酸酶）。第一和另外的样品中的靶分子可以被不同地标记，例如。通过携带不同的标签。

4. 发明申请

WO2009034181A3 METHODS FOR THE PREPARATION OF DNA MICROARRAYS WITH LINEAR HIGH DENSITY PROBES 审中-公开
标题翻译：用线性高密度探针制备DNA微阵列的方法
公开(公告)号：WO2009034181A3
公开(公告)日：2009-04-30
申请号：PCT/EP2008062207
申请日：2008-09-12
申请人： TWOF INC , CUPPOLETTI ANDREA , STELLACCI FRANCESCO , LARMAN HARRY BENJAMIN , PISANELLI BARBARA
发明人： CUPPOLETTI ANDREA , STELLACCI FRANCESCO , LARMAN HARRY BENJAMIN , PISANELLI BARBARA
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6834 , C12Q2565/515 , C12Q2531/125
摘要： A method for the realisation of DNA microarrays with linear high density probes, comprising a phase of replication of template DNA strands of a template microarray and a subsequent phase of stamping of the molecules obtained by replication on a substrate via the SuNS technique; the template strands (21, 38, 44) of said template microarray are either composed of or derived from a rolling circle (21; 38; 43).
摘要翻译：一种用线性高密度探针实现DNA微阵列的方法，其包括模板微阵列的模板DNA链的复制阶段和通过SuNS技术在底物上通过复制获得的分子的冲压的后续阶段; 所述模板微阵列的模板链（21,38,44）由滚动圆（21; 38; 43）组成或衍生自滚动圆（21; 38; 43）。

5. 发明申请

WO2008039071A3 HIGH-THROUGHPUT DIAGNOSTIC TESTING USING ARRAYS 审中-公开
标题翻译：使用阵列的高通量诊断测试
公开(公告)号：WO2008039071A3
公开(公告)日：2008-09-12
申请号：PCT/NL2007050473
申请日：2007-09-28
申请人： AGENDIA B V , GLAS ANNUSKA MARIA , FLOORE ARNO NICOLAAS , VAN T VEER LAURA JOHANNA
发明人： GLAS ANNUSKA MARIA , FLOORE ARNO NICOLAAS , VAN T VEER LAURA JOHANNA
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6837 , C12Q2600/158 , C12Q2565/515 , C12Q2545/101 , C12Q2537/165
摘要： The present invention relates to arrays comprising between 2 and 12.000 nucleic acid molecules, comprising a first set of nucleic acid molecules that comprise a nucleotide sequence that is able to hybridize to a gene that is used for normalization. The array may further comprise a second set of nucleic acid molecules that comprise nucleic acid sequences capable of hybridizing to nucleic acid molecules that are expressed in clinical relevant samples such as, for example, breast tissue. The invention further relates to a method for normalizing data. Further provided are methods of using an array according to the invention for distinguishing clinical samples.
摘要翻译：本发明涉及包含2至12.000个核酸分子的阵列，其包含第一组核酸分子，其包含能够与用于标准化的基因杂交的核苷酸序列。阵列还可以包含第二组核酸分子，其包含能够与在临床相关样品例如乳腺组织中表达的核酸分子杂交的核酸序列。本发明还涉及一种用于规范化数据的方法。还提供了使用根据本发明的阵列区分临床样品的方法。

6. 发明申请

WO2004067759A3 REPLICATION OF NUCLEIC ACID ARRAYS 审中-公开
标题翻译：核酸阵列的复制
公开(公告)号：WO2004067759A3
公开(公告)日：2004-11-18
申请号：PCT/GB2004000375
申请日：2004-01-29
申请人： UNIV CRANFIELD , BOSSI ALESSANDRA , PILETSKY SERGEY ANATOLIYOVICH , PILETSKA OLENA VOLODIMIRIVNA , WARNER PHILIP JAMES , TURNER ANTHONY PETER FRANCIS
发明人： BOSSI ALESSANDRA , PILETSKY SERGEY ANATOLIYOVICH , PILETSKA OLENA VOLODIMIRIVNA , WARNER PHILIP JAMES , TURNER ANTHONY PETER FRANCIS
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q2525/161 , C12Q2565/537 , C12Q2565/515
摘要： A method of producing arrays of nucleic acids corresponding to a first set of nucleic acids immobilised on a first solid support (master array) includes furnishing on said first set of nucleic acids a second set of nucleic acids, each nucleic acid of the second set having at least a portion which is complementary to at least a portion of respective nucleic acid of the first set and being hybridised to it thereby. This may involve contact with a solution containing a mixture of nucleic acids, or synthesis using the first nucleic acids as primers and templates. The second set of nucleic acids are immobilised to a second solid support, either before or while they are hybridised to the first set of nucleic acids. Separation of the first and second solid supports provides said first support bearing the master array and said second support bearing a complementary array.
摘要翻译：产生对应于固定在第一固体支持物（主阵列）上的第一组核酸的核酸阵列的方法包括在所述第一组核酸上提供第二组核酸，第二组的每个核酸具有至少一部分与第一组核酸的至少一部分互补并由此与其杂交。这可能涉及与含有核酸混合物的溶液接触，或者使用第一核酸作为引物和模板进行合成。在第二组核酸与第一组核酸杂交之前或之时，将第二组核酸固定到第二固体支持物上。第一和第二固体支撑件的分离提供了所述第一支撑件支撑主阵列，并且所述第二支撑件支撑互补阵列。

7. 发明申请

WO2004038415A1 ASSAY WITH CO-IMMOBILIZED LIGANDS 审中-公开
标题翻译：用固定配方进行测定
公开(公告)号：WO2004038415A1
公开(公告)日：2004-05-06
申请号：PCT/SE2003/001617
申请日：2003-10-21
申请人： BIACORE AB , RICHALET-SECORDEL, Pascale , MULHOLLAND, Stephen, E. , ANDERSSON, Karl
发明人： RICHALET-SECORDEL, Pascale , MULHOLLAND, Stephen, E. , ANDERSSON, Karl
IPC分类号： G01N33/543
CPC分类号： G01N33/543 , C12Q1/6837 , C40B30/04 , C12Q2565/515 , C12Q2537/143 , C12Q2565/513
摘要： Multivariate type assay methods are disclosed which comprise co-immobilizing different groups of ligands on respective discrete areas of a solid support surface, sequentially contacting the different areas with single or multiple analytes, detecting interactions of the analytes with the ligand groups, and determining therefrom the amount of ligand-binding of each analyte.
摘要翻译：公开了多变量型测定方法，其包括在固体支持物表面的相应离散区域上共同固定不同组配体，依次使不同区域与单个或多个分析物接触，检测分析物与配体基团的相互作用，每种分析物的配体结合量。

8. 发明申请

WO03066884A2 METHOD FOR LABELING ARTICLES 审中-公开
标题翻译：程序的对象标记
公开(公告)号：WO03066884A2
公开(公告)日：2003-08-14
申请号：PCT/EP0301186
申请日：2003-02-06
申请人： NANOTYPE GMBH , OESTERHELT FILIPP , MAI THAO , SPINNLER KATRIN , BLANK KERSTIN , MUELLNER STEFAN
发明人： OESTERHELT FILIPP , MAI THAO , SPINNLER KATRIN , BLANK KERSTIN , MUELLNER STEFAN
IPC分类号： C12Q1/68 , C12Q1/00
CPC分类号： C12Q1/6834 , C12Q2565/515 , C12Q2563/185
摘要： The invention relates to a method for proving the authenticity of an article. The article, on one of its surfaces, has a section on which partners of a specifically binding pair are bound in a predetermined arrangement. The surface is contacted with a second surface that has corresponding sections on which, in at least selected areas, complementary binding partners are disposed, in such a manner that the partners react with one another, at least a part of the partners carrying a detectable label. The surfaces are then separated and the arrangement of labels so produced on at least one of the two surfaces is detected. After separation, the arrangement of partners and/or labels produced on at least one of the two surfaces differs from the original arrangement of the bound partners and/or of the labels on the surface of the article.
摘要翻译：本发明涉及一种用于证明物品的真实性的方法。的制品，在其表面中的一个，具有一个特异性结合对哪个伙伴的区段以预定的布置的约束。表面与第二表面接触那样具有在其上相应部分，在至少选定的区域，互补结合伴侣在寻求的方式没有伙伴彼此，至少一个携带可检测标记的伙伴的一部分发生反应处理完毕，然后，将表面被分离，并当检测到对所述两个表面中的至少一个如此产生标签的布置。分离之后，对两个表面中的至少一个产生的合作伙伴和/或标签的排列从结合的合作伙伴和/或物品的表面上的标签上的原来的安排是不同的。

9. 发明申请

WO0053812A3 REPLICA AMPLIFICATION OF NUCLEIC ACID ARRAYS 审中-公开
标题翻译：核酸序列的补体放大
公开(公告)号：WO0053812A3
公开(公告)日：2002-07-11
申请号：PCT/US0006390
申请日：2000-03-10
申请人： HARVARD COLLEGE
发明人： CHURCH GEORGE M , MITRA ROBI D
IPC分类号： G01N31/22 , B01J19/00 , B05D3/00 , B32B5/02 , C07H19/00 , C07H21/00 , C07H21/02 , C07H21/04 , C12M1/34 , C12N15/09 , C12P19/34 , C12Q1/68 , G01N15/06 , G01N21/78 , G01N33/53 , G01N33/58 , G01N37/00
CPC分类号： C12Q1/6837 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00641 , B01J2219/00644 , B01J2219/00657 , B01J2219/00702 , B01J2219/00722 , C12Q1/6874 , C12Q2600/156 , C12Q2565/515 , C12Q2565/507 , C12Q2531/119
摘要： Disclosed are improved methods of making and using immobilized arrays of nucleic acids, particularly methods for producing replicas of such arrays. Included are methods for producing high density arrays of nucleic acids and replicas of such arrays, as well as methods for preserving the resolution of arrays through rounds of replication. Also included are methods which take advantage of the availability of replicas of arrays for increased sensitivity in detection of sequences on arrays. Improved methods of sequencing nucleic acids immobilized on arrays utilizing single copies of arrays and methods taking further advantage of the availability of replicas of arrays are disclosed. The improvements lead to higher fidelity and longer read lengths of sequences immobilized on arrays. Methods are also disclosed which improve the efficiency of multiplex PCR using arrays of immobilized nucleic acids.
摘要翻译：公开了制备和使用固定化核酸阵列的改进方法，特别是用于制备这种阵列复制品的方法。包括用于生产核酸的高密度阵列和这种阵列的复制品的方法，以及通过复制复制来保持阵列分辨率的方法。还包括利用阵列副本的可用性的方法，以增加在阵列上的序列检测中的灵敏度。公开了使用单个阵列副本固定在阵列上的核酸测序的改进方法，以及进一步利用阵列副本的可用性的方法。这些改进导致固定在阵列上的序列的更高保真度和更长的读取长度。还公开了使用固定化核酸阵列提高多重PCR的效率的方法。

10. 发明申请

WO00032809A2 CLONING AND COPYING ON SURFACES 审中-公开
标题翻译： COPY和克隆表面上
公开(公告)号：WO00032809A2
公开(公告)日：2000-06-08
申请号：PCT/DE1999/003856
申请日：1999-11-26
IPC分类号： G01N33/53 , C12N15/09 , C12N15/10 , C12Q1/68 , C40B30/04 , C40B60/12 , G01N33/566 , G01N37/00
CPC分类号： C12N15/10 , C12Q1/686 , Y10S977/902 , C12Q2565/543 , C12Q2565/515 , C12Q2565/125
摘要： The invention relates to a method for cloning and copying genetic material on surfaces as well as copying biological material insofar as it, in a broader sense, can be classified in a ligand receptor system. The invention thus relates, in particular, to a method for propagating ligands and receptors on at least two surfaces which comprises one or several of the following cycles: a) Immobilizing a ligand on a first surface of a solid phase; b) adding a solution of receptors and binding complementary receptors to the ligands; c) transferring the receptor to an additional surface and immobilizing the receptor at that location; d) attaching an additional ligand to the immobilized receptor; e) transferring the ligand to a surface and immobilizing the same at that location. Nucleic acids are also understood as a ligand/receptor system.
摘要翻译：本发明涉及用于在表面上和克隆复制的遗传物质的方法，而且还生物材料的拷贝，在某种程度上可以在广义上被分类在配体 - 受体系统。因此，本发明更具体地涉及用于传播的至少两个表面上的配体和受体，包括以下循环中的一个或多个的方法：a）在固相的第一表面上的配体的固定化; b）加入受体中的溶液和结合互补受体的配体; c）该受体的附加表面和受体的本地固定的转移; D）加入另外的配体与固定化受体的; E）配体转移到配体的表面上并局部固定。作为配体/受体系统还核酸理解。

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