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1. 发明申请

WO2019060801A1 TELOMERE FUSIONS AND THEIR DETECTION OF DYSPLASIA AND/OR CANCER 审中-公开
公开(公告)号：WO2019060801A1
公开(公告)日：2019-03-28
申请号：PCT/US2018/052342
申请日：2018-09-24
申请人： THE JOHNS HOPKINS UNIVERSITY
发明人： GOGGINS, Michael , HATA, Tatsuo
IPC分类号： C12Q1/686 , C12Q1/6827 , C12Q1/6844 , C12Q1/6883 , C12Q1/6886
CPC分类号： C12Q1/6886 , C12Q1/6827 , C12Q1/6844 , C12Q1/686 , C12Q1/6883 , C12Q2600/156 , C12Q2549/119
摘要： Described are telomere fusion assays to detect dysplasia in subjects using nested primers. The methods of the present invention are able to detect dysplasia in subjects with intraductal papillary mucinous neoplasma (IPMNs), for example.

2. 发明申请

WO2017009693A1 16S RIBOSOMAL RNA UNIVERSAL PRIMERS AND USE THEREOF IN MICROBIOLOGICAL ANALYSIS AND DIAGNOSTICS 审中-公开
标题翻译： 16S RIBOSOMAL RNA通用引物及其在微生物学分析与诊断中的应用
公开(公告)号：WO2017009693A1
公开(公告)日：2017-01-19
申请号：PCT/IB2015/056715
申请日：2015-09-03
申请人： UNIWERSYTET JAGIELLOŃSKI
发明人： GOSIEWSKI, Tomasz , SALAMON, Domininka , BULANDA, Małgorzata , RADKOWSKI, Piotr , LUDWIG-GAŁĘZOWSKA, Agnieszka , WOŁKOW, Paweł , SROKA, Agnieszka
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/686 , C12Q1/6869 , C12Q2549/119 , C12Q2561/113 , C12Q2600/16 , G01N33/56911
摘要： The subject-matter of the invention is a pair of primers, a method of microbiological analysis of biomaterial, application of NGS sequencing method in microbiological diagnostics of blood and diagnostics set. An innovative method of body fluids diagnosing from microbiological perspective i.e. complex analysis of bacterial profiles in the samples was developed in more detailed manner.
摘要翻译：本发明的主题是一对引物，生物材料的微生物分析方法，NGS测序方法在血液和诊断集的微生物诊断中的应用。从微生物角度诊断体液的创新方法，即以更详细的方式开发了样品中细菌谱的复杂分析。

3. 发明申请

WO2013009084A2 COMPOSITION FOR SIMULTANEOUSLY DETECTING MYCOBACTERIUM TUBERCULOSIS AND NONTUBERCULOUS MYCOBACTERIA BY MEANS OF REAL-TIME MULTIPLEX POLYMERASE CHAIN REACTION COMPRISING NESTED HYBRIDIZATION PNA PROBE SYSTEM HAVING PARALLEL BINDING STRUCTURE, AND METH 审中-公开
标题翻译：通过实时多重聚合酶链反应，同时检测MYCOBACTERIUM TUBCCOSOSIS和NONTUBERCULOUS MYCOBACTERIA的组合物，其包含具有平行结合结构的NESTED混合PNA探针系统和METH
公开(公告)号：WO2013009084A2
公开(公告)日：2013-01-17
申请号：PCT/KR2012005483
申请日：2012-07-11
申请人： PANAGENE INC , KIM SUNG-KEE , PARK CHWANG SIEK , JOE GOON HO , PARK HEEKYUNG , KIM JIHYUN , CHOI JI-AH , CHOI SUNG ROK , KIM SU NAM , KIM YONGTAE , KIM SE RYUN , JANG HYUNJUNG
发明人： KIM SUNG-KEE , PARK CHWANG SIEK , JOE GOON HO , PARK HEEKYUNG , KIM JIHYUN , CHOI JI-AH , CHOI SUNG ROK , KIM SU NAM , KIM YONGTAE , KIM SE RYUN , JANG HYUNJUNG
IPC分类号： C12N15/11 , C12Q1/04 , C12Q1/68
CPC分类号： C12Q1/689 , C12Q1/6851 , C12Q2600/16 , G01N2333/35 , C12Q2525/107 , C12Q2537/143 , C12Q2549/119 , C12Q2561/113
摘要： The present invention relates to a composition for simultaneously detecting Mycobacterium tuberculosis and nontuberculous mycobacteria based on a nested hybridization peptide nucleic acid (PNA) probe system having a parallel binding structure, and to a method for simultaneously detecting Mycobacterium tuberculosis and nontuberculous mycobacteria using the composition and real-time polymerase chain reaction. Also, the present invention relates to a primer, a probe composition, and a method for analyzing to simultaneously detect Mycobacterium tuberculosis and nontuberculous mycobacteria based on the nested hybridization PNA probe system having the parallel binding structure. When carrying out the real-time multiplexed polymerase chain reaction by using the composition comprising the nested hybridized PNA probe system having the parallel binding structure, according to the present invention, Mycobacterium tuberculosis and nontuberculous mycobacteria can be simultaneously and accurately detected in a single tube with high sensitivity, thereby providing the advantage of making a more rapid and accurate clinical diagnosis.
摘要翻译：本发明涉及一种基于具有平行结合结构的嵌套杂交肽核酸（PNA）探针系统同时检测结核分枝杆菌和非结核分枝杆菌的组合物，以及使用该组合物同时检测结核分枝杆菌和非结核分枝杆菌的方法，实时聚合酶链反应。此外，本发明涉及基于具有平行结合结构的嵌套杂交PNA探针系统的引物，探针组合物和分析方法，以同时检测结核分枝杆菌和非结核分枝杆菌。当通过使用包含具有平行结合结构的嵌套杂交的PNA探针系统的组合物进行实时多重聚合酶链反应时，根据本发明，结核分枝杆菌和非结核分枝杆菌可以在单个管中同时且准确地检测，灵敏度高，从而提供了更迅速和准确的临床诊断的优点。

4. 发明申请

WO2012159060A2 METHODS, SYSTEMS, AND COMPOSITIONS FOR DETECTION OF MICROBIAL DNA BY PCR 审中-公开
标题翻译：通过PCR检测微生物DNA的方法，系统和组合物
公开(公告)号：WO2012159060A2
公开(公告)日：2012-11-22
申请号：PCT/US2012038649
申请日：2012-05-18
申请人： DYNOCUBE INVESTMENTS LLC , TSENG CHING-PING
发明人： TSENG CHING-PING , CHANG SHY-SHIN
IPC分类号： C40B30/04 , C07H21/04 , C40B40/06 , C40B60/12
CPC分类号： C12Q1/6848 , C12Q2525/155 , C12Q2525/161 , C12Q2549/119
摘要： This invention provides methods, systems, and compositions for detecting low abundance microbial DNA in a sample by broad-range PCR. Methods of the present invention are based on a novel strategy that tags the 5 '-end of the target DNA templates with a non-bacterial tagging sequence so as to set the templates apart from the endogenous contaminants present in the PCR reagents. There is also provided fusion probes for tagging the templates and primer sets to amplify the tagged templates. Systems and kits for facilitating and automating methods of the present invention are also provided.
摘要翻译：本发明提供了通过广范围PCR检测样品中低丰度微生物DNA的方法，系统和组合物。本发明的方法是基于用非细菌标签序列标记目标DNA模板的5'末端以便将模板与存在于PCR试剂中的内源性污染物分开的新策略。还提供了用于标记模板和引物组的融合探针以扩增标记的模板。还提供了用于促进和自动化本发明方法的系统和套件。

5. 发明申请

WO2011056133A1 GENOTYPING 审中-公开
标题翻译：基因分型
公开(公告)号：WO2011056133A1
公开(公告)日：2011-05-12
申请号：PCT/SE2010/051200
申请日：2010-11-03
申请人： SVA STATENS VETERINÄRMEDICINSKA ANSTALT , LEIJON, Mikael , BELÁK, Sándor
发明人： LEIJON, Mikael , BELÁK, Sándor
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q2549/119 , C12Q2537/143 , C12Q2535/125 , C12Q2563/131 , C12Q2531/107 , C12Q2525/155 , C12Q2527/143 , C12Q2563/107 , C12Q2563/179
摘要： The object of the present invention is to provide a novel method that addresses the problem of differentiating large sets of genomic sequences. The invention relates to a method for genotyping N loci present in a sample in a target nucleic acid molecule, wherein each locus is located in a genotype marker region of the nucleic acid molecule, and corresponds to two or more genotypes. Furthermore the invention relates to a kit for performing said method for genotyping. Also the invention relates to a method for designing and producing selection primers, as well as a method for producing detection primers, all of said primers to be used in the method for genotyping or in the kit for performing the genotyping method. The invention further relates to selection primers adapted for genotyping of loci in a target nucleic acid molecule and a computer program product for designing selection primers.
摘要翻译：本发明的目的是提供一种解决区分大量基因组序列的问题的新方法。本发明涉及一种用于对存在于目标核酸分子中的样品中存在的N个基因座进行基因分型的方法，其中每个基因座位于核酸分子的基因型标记区域中，并且对应于两种或更多种基因型。此外，本发明涉及用于进行所述基因分型方法的试剂盒。本发明还涉及用于设计和制备选择性引物的方法，以及用于产生检测引物的方法，所有用于基因分型方法的所述引物或用于进行基因分型方法的试剂盒。本发明还涉及适用于靶核酸分子中基因座基因分型的选择引物和用于设计选择引物的计算机程序产品。

6. 发明申请

WO2010139010A1 A METHOD OF NUCLEIC ACID AMPLIFICATION 审中-公开
标题翻译：一种核酸扩增方法
公开(公告)号：WO2010139010A1
公开(公告)日：2010-12-09
申请号：PCT/AU2010/000680
申请日：2010-06-02
申请人： MONOQUANT PTY LTD , MORLEY, Alexander, Alan , BRISCO, Michel Julian
发明人： MORLEY, Alexander, Alan , BRISCO, Michel Julian
IPC分类号： C12P19/34
CPC分类号： C12Q1/686 , C12Q2549/119 , C12Q2527/107
摘要： The present invention relates generally to a method of amplifying a nucleic acid region of interest and, more particularly, to a method of amplifying a nucleic acid region of interest via a nested single tube PCR. The method of the invention is designed to provide a means to selectively inactivate the functionality of the outer primer or primers via an antisense nucleic acid molecule targeted to at least one of the outer primers, thereby maintaining amplification efficiency throughout the reaction. The development of a means to achieve efficient amplification by the outer primer followed by efficient amplification with the inner primers, in the context of a single tube nested PCR, is useful in a range of applications including, but not limited to, the diagnosis and/or monitoring of disease conditions which are characterised by specific gene sequences and the characterisation or analysis of specific gene regions of interest. Still further, the method of the present invention enables quantification to be performed and not just simple detection.
摘要翻译：本发明一般涉及扩增感兴趣的核酸区域的方法，更具体地涉及通过嵌套单管PCR扩增目的核酸区域的方法。本发明的方法被设计为提供通过靶向至少一种外部引物的反义核酸分子选择性地失活外部引物或引物的功能的手段，由此保持整个反应中的扩增效率。在单管嵌套PCR的背景下，通过外部引物实现有效扩增，然后用内部引物进行有效扩增的手段的开发在一系列应用中是有用的，包括但不限于诊断和/ 或监测以特定基因序列为特征的疾病状况，以及特定基因区域的表征或分析。此外，本发明的方法能够进行定量，而不仅仅是简单的检测。

7. 发明申请

WO2010094475A1 METHOD FOR OBTAINING IMMUNOGLOBULIN ENCODING NUCLEIC ACID 审中-公开
标题翻译：获得免疫球蛋白编码核酸的方法
公开(公告)号：WO2010094475A1
公开(公告)日：2010-08-26
申请号：PCT/EP2010/001008
申请日：2010-02-18
申请人： F. HOFFMANN-LA ROCHE AG , KRELL, Hans Willi , LIFKE, Alexander , LIFKE, Valeria , MADIN, Kairat , WEILKE, Christian
发明人： KRELL, Hans Willi , LIFKE, Alexander , LIFKE, Valeria , MADIN, Kairat , WEILKE, Christian
IPC分类号： C12Q1/68 , C07K16/00
CPC分类号： C12P21/005 , C07K16/00 , C07K2317/14 , C07K2317/21 , C07K2317/55 , C12P19/34 , C12Q1/6804 , C12Q1/6858 , C12Q1/6881 , C12Q2600/16 , C12Q2549/119 , C12Q2537/143
摘要： The current invention is directed to a method for obtaining a nucleic acid encoding an immunoglobulin variable domain from a single cell comprising the following steps: - performing a first polymerase chain reaction with three to six 5'-primer and one 3'-primer, performing with the product of the first polymerase chain reaction a second polymerase chain reaction with thirteen to sixteen 5'-primer and one 3'-primer, whereby the distance of the binding locations of the primer employed in the second polymerase chain reaction is reduced compared to the first polymerase chain reaction.
摘要翻译：本发明涉及从单细胞获得编码免疫球蛋白可变结构域的核酸的方法，包括以下步骤： - 用3至6个5'-引物和1个3'-引物进行第一聚合酶链反应，进行与第一聚合酶链反应的产物进行第二聚合酶链反应与十三至十六个5'-引物和一个3'-引物，由此在第二聚合酶链式反应中使用的引物的结合位置的距离比第一次聚合酶链反应。

8. 发明申请

WO2010014920A1 CIRCULATING MUTANT DNA TO ASSESS TUMOR DYNAMICS 审中-公开
标题翻译：循环突变DNA评估肿瘤动力学
公开(公告)号：WO2010014920A1
公开(公告)日：2010-02-04
申请号：PCT/US2009/052436
申请日：2009-07-31
申请人： THE JOHNS HOPKINS UNIVERSITY , DIEHL, Frank , DIAZ, Luis , KINZLER, Kenneth, W. , VOGELSTEIN, Bert , SCHMIDT, Kerstin
发明人： DIEHL, Frank , DIAZ, Luis , KINZLER, Kenneth, W. , VOGELSTEIN, Bert , SCHMIDT, Kerstin
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2525/197 , C12Q2535/131 , C12Q2549/119 , C12Q2600/112 , C12Q2600/118 , C12Q2600/136
摘要： DNA containing somatic mutations is highly tumor specific and thus, in theory, can provide optimum markers. However, the number of circulating mutant gene fragments is small compared to the number of normal circulating DNA fragments, making it difficult to detect and quantify them with the sensitivity required for meaningful clinical use. We apply a highly sensitive approach to quantify circulating tumor DNA (ctDNA) in body samples of patients. Measurements of ctDNA can be used to reliably monitor tumor dynamics in subjects with cancer, especially those who are undergoing surgery or chemotherapy. This personalized genetic approach can be generally applied.
摘要翻译：含有体细胞突变的DNA具有高度的肿瘤特异性，因此在理论上可以提供最佳的标记。然而，循环突变基因片段的数量与正常循环DNA片段的数量相比较小，使得难以用有意义的临床使用所需的灵敏度来检测和量化它们。我们应用高度敏感的方法来量化患者身体样本中的循环肿瘤DNA（ctDNA）。 ctDNA的测量可用于可靠地监测癌症患者的肿瘤动态，特别是正在接受手术或化疗的患者。这种个性化的遗传方法一般可以应用。

9. 发明申请

WO2009036514A3 METHOD OF AMPLIFYING NUCLEIC ACID 审中-公开
标题翻译：放大核酸的方法
公开(公告)号：WO2009036514A3
公开(公告)日：2009-06-04
申请号：PCT/AU2008001396
申请日：2008-09-19
申请人： MOLECULAR PLANT BREEDING NOMINEES LTD , HAYDEN MATTHEW JAMES , TABONE TANIA
发明人： HAYDEN MATTHEW JAMES , TABONE TANIA
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： C12Q1/6858 , C12Q2549/119 , C12Q2535/125
摘要： The present invention provides a method for detecting a polymorphism or mutation in nucleic acid comprising a first phase to amplify or enrich for a sequence comprising a polymorphism or mutation and a second phase for detecting the polymorphism or mutation, wherein both phases are performed in the same reaction vessel.
摘要翻译：本发明提供了一种检测核酸多态性或突变的方法，其包括第一相以扩增或富集包含多态性或突变的序列和用于检测多态性或突变的第二相，其中两相进行相同反应容器

10. 发明申请

WO2009036514A2 METHOD OF AMPLIFYING NUCLEIC ACID 审中-公开
标题翻译：放大核酸的方法
公开(公告)号：WO2009036514A2
公开(公告)日：2009-03-26
申请号：PCT/AU2008/001396
申请日：2008-09-19
申请人： MOLECULAR PLANT BREEDING NOMINEES LTD , HAYDEN, Matthew James , TABONE, Tania
发明人： HAYDEN, Matthew James , TABONE, Tania
CPC分类号： C12Q1/6858 , C12Q2549/119 , C12Q2535/125
摘要： The present invention provides a method for detecting a polymorphism or mutation in nucleic acid comprising a first phase to amplify or enrich for a sequence comprising a polymorphism or mutation and a second phase for detecting the polymorphism or mutation, wherein both phases are performed in the same reaction vessel.
摘要翻译：本发明提供了用于检测核酸中的多态性或突变的方法，所述方法包括扩增或富集包含多态性或突变的序列的第一阶段和用于检测多态性或突变的第二阶段，其中两个相在相同的反应容器中进行。

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