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1. 发明申请

WO2011082419A3 DNA SEQUENCING METHODS AND DETECTORS AND SYSTEMS FOR CARRYING OUT THE SAME 审中-公开
标题翻译： DNA序列方法和检测器及其执行系统
公开(公告)号：WO2011082419A3
公开(公告)日：2012-02-09
申请号：PCT/US2011020117
申请日：2011-01-04
申请人： LIFE TECHNOLOGIES CORP , SUN HONGYE , NORDMAN ERIC S , OLDHAM MARK F , O'NEILL JOHN R , CONNELL CHARLES , ULMANELLA UMBERTO , LAU ALDRICH N K , KOTSEROGLOU THEOFILOS , LIVAK KENNETH J
发明人： SUN HONGYE , NORDMAN ERIC S , OLDHAM MARK F , O'NEILL JOHN R , CONNELL CHARLES , ULMANELLA UMBERTO , LAU ALDRICH N K , KOTSEROGLOU THEOFILOS , LIVAK KENNETH J
IPC分类号： C12Q1/68 , G01N27/414 , G01N33/50
CPC分类号： C12Q1/6869 , B01L3/502761 , G01N27/3278 , G01N27/414 , G01N27/4145 , G01N27/4146 , G01N33/5438 , Y10S977/924 , Y10T436/143333 , C12Q2565/631
摘要： In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures. Surface-modified nanopores are provided as are methods of making them. In some embodiments, surfaced-modified nanopores for slowing the translocation of single stranded DNA (ssDNA) through the nanopore are provided, as are nanopores configured to detect each of a plurality of different bases on an ssDNA strand.
摘要翻译：在一些实施例中，提供了分析物检测系统，其包括纳米通道，电极装置和设置在纳米通道中的多个纳米器件。可以使用多个核酸碱基检测组分，其包括多个纳米孔，多个纳米通道，多个杂交探针及其组合等。根据本教导的其他实施方案，将不同的编码分子与靶DNA分子杂交并用于检测沿着靶分子的各种序列的存在。还提供了包含编码分子混合物的试剂盒。在一些实施方案中，包括纳米通道，纳米孔等的装置用于操纵DNA分子的移动，例如用于DNA测序检测的准备。还提供了制备其的纳米孔结构及其制备方法，使用纳米孔结构的核酸测序方法也是如此。提供表面改性的纳米孔也是制备它们的方法。在一些实施方案中，提供用于减缓单链DNA（ssDNA）穿过纳米孔的易位的表面改性的纳米孔，以及配置成检测ssDNA链上的多个不同碱基中的每一个的纳米孔。

2. 发明申请

WO2009079052A3 DEVICES AND METHODS FOR THERMALLY ISOLATING CHAMBERS OF AN ASSAY CARD 审中-公开
标题翻译：测定卡热隔离器的装置和方法
公开(公告)号：WO2009079052A3
公开(公告)日：2009-08-20
申请号：PCT/US2008077092
申请日：2008-09-19
申请人： APPLIED BIOSYSTEMS , YANG JOON MO , LIU DAVID M , CHIANG YUH-MIN , SCHEMBRI CAROL , LAU ALDRICH N K , ULMANELLA UMBERTO , BEARD NIGEL P , SHARIATI MARYAM , NURSE JAMES C , HAGA DOUWE D , HARDING IAN A , FOCARACCI JULIO P
发明人： YANG JOON MO , LIU DAVID M , CHIANG YUH-MIN , SCHEMBRI CAROL , LAU ALDRICH N K , ULMANELLA UMBERTO , BEARD NIGEL P , SHARIATI MARYAM , NURSE JAMES C , HAGA DOUWE D , HARDING IAN A , FOCARACCI JULIO P
IPC分类号： B01L3/00
CPC分类号： B01L3/502707 , B01L7/52 , B01L2200/141 , B01L2300/0816 , B01L2300/1805 , B01L2300/1861 , B01L2400/0677
摘要： An assay card and devices and methods for isolating chambers on the assay card are described. The assay card comprises a substrate formed of one or more materials, e.g., plastic, having a softening temperature, the substrate defining channels communicating with respective reaction chambers. The assay card may be heated in a region of the channels to at least the softening temperature. The softened plastic may be deformed, e.g., with a tool which may or may not also provide the heat for softening the substrate. In this manner, the plastic of the substrate may be caused to at least partially obstruct the channels, thereby isolating the reaction chambers. The invention also relates to a method of manufacturing a tool device that includes pins for heating and deforming an assay card.
摘要翻译：描述了用于在测试卡上隔离室的测定卡和装置和方法。测定卡包括由一种或多种具有软化温度的材料（例如塑料）形成的衬底，衬底限定通道与各个反应室连通。测定卡可以在通道的区域中被加热到至少软化温度。软化的塑料可能变形，例如用可能也可能不提供用于软化基底的热量的工具。以这种方式，可以使衬底的塑料至少部分地阻碍通道，从而隔离反应室。本发明还涉及一种制造工具装置的方法，该工具装置包括用于加热和变形分析卡的销。

3. 发明申请

WO2008144288A8 APPARATUS AND METHODS FOR PREPARATION OF SUBTANTIALLY UNIFORM EMULSIONS CONTAINING A PARTICLE 审中-公开
标题翻译：用于制备含有颗粒的亚基均匀乳液的装置和方法
公开(公告)号：WO2008144288A8
公开(公告)日：2009-06-04
申请号：PCT/US2008063480
申请日：2008-06-06
申请人： APPLERA CORP , LAU ALDRICH N K , CASSEL JONATHAN M , BRIDGHAM JOHN A
发明人： LAU ALDRICH N K , CASSEL JONATHAN M , BRIDGHAM JOHN A
IPC分类号： A61K8/06 , B01J13/00
CPC分类号： B01F3/0807 , B01F5/0475 , B01F5/0476 , B01F5/0485 , B01F13/0059
摘要： Methods and systems for forming water-in-oil emulsions are described. For example, an apparatus is described which includes: a first compartment containing a plurality of particles dispersed in an aqueous phase; a second compartment containing an oil phase; a porous layer separating the first and second compartments; and a device for applying pressure to the first compartment. A method is described which includes: moving an oil phase relative to a surface of a porous layer while simultaneously forcing an aqueous composition comprising particles through the porous layer and into the flowing dispersion medium thereby forming droplets of the aqueous composition containing particles dispersed in the oil phase. The aqueous composition can include one or more nucleic acid templates and reagents for amplifying the nucleic acids such as PCR reagents. A porous partition is described comprising a first and second major surfaces and at least two straight through pores comprising a cross sectional shape selected from a polygon, an oval, an oblong, a dumbbell, a bowtie and irregular shapes thereof. Aqueous droplets containing an oligonucleotide attached to a particle and reagents can be used as a microreactor for nucleic acid amplification.
摘要翻译：描述了形成油包水乳液的方法和系统。例如，描述了一种装置，其包括：包含分散在水相中的多个颗粒的第一隔室; 含有油相的第二隔室; 分离第一和第二隔室的多孔层; 以及用于向第一隔室施加压力的装置。描述了一种方法，其包括：相对于多孔层的表面移动油相，同时迫使包含颗粒的水性组合物通过多孔层并进入流动分散介质中，由此形成含有分散在油中的颗粒的含水组合物的液滴相。水性组合物可以包括一种或多种核酸模板和用于扩增核酸如PCR试剂的试剂。描述了一种多孔分隔件，其包括第一和第二主表面和至少两个直通孔，其包括选自多边形，椭圆形，长方形，哑铃，弓形和不规则形状的横截面形状。含有与粒子连接的寡核苷酸的水滴和试剂可以用作用于核酸扩增的微反应器。

4. 发明申请

WO2007050143A3 SURFACE MODIFICATION IN A MANIPULATION CHAMBER 审中-公开
标题翻译：操纵室中的表面修饰
公开(公告)号：WO2007050143A3
公开(公告)日：2009-05-14
申请号：PCT/US2006022959
申请日：2006-06-13
申请人： APPLERA CORP , LAU ALDRICH N K , PHAN HUAN L
发明人： LAU ALDRICH N K , PHAN HUAN L
IPC分类号： C12M1/34 , C12M3/00
CPC分类号： G01N27/305 , B03C11/00 , C07K1/26
摘要： A device for manipulating biological material, the device including at least one electrode and a photoconductive material configured to receive the biological material; and a light source configured to illuminate the photoconductive material so as to modulate an electric field, wherein the electric field is configured to manipulate the biological material; wherein a surface of the at least one electrode and/or the photoconductive material is modified with at least one of a carboxylic moiety, an amino moiety, a poly(ethylene glycol) moiety, a polymer of (poly(ethylene oxide) methyl ether) acrylate, a poly(2-hydroxyethyl (meth)acrylate), a poly(N-vinylpyrrolidone), a poly(N-vinylformamide), a poly(N-vinylformamide) derivative, a poly((meth)acrylamide), and a poly((meth)acrylamide) derivative. Methods for manipulating biological material are also disclosed.
摘要翻译：一种用于操纵生物材料的装置，该装置包括至少一个电极和被配置为容纳生物材料的光导材料; 以及光源，被配置为照亮所述光电导材料以调制电场，其中所述电场被配置为操纵所述生物材料; 其中所述至少一个电极和/或光电导材料的表面用羧基部分，氨基部分，聚（乙二醇）部分，（聚（环氧乙烷）甲基醚）的聚合物，丙烯酸酯，聚（甲基）丙烯酸2-羟乙酯），聚（N-乙烯基吡咯烷酮），聚（N-乙烯基甲酰胺），聚（N-乙烯基甲酰胺）衍生物，聚（（甲基）丙烯酰胺）聚（（甲基）丙烯酰胺）衍生物。还公开了用于操作生物材料的方法。

5. 发明申请

WO2007001436A3 SURFACE MODIFICATION FOR NON-SPECIFIC ADSORPTION OF BIOLOGICAL MATERIAL 审中-公开
公开(公告)号：WO2007001436A3
公开(公告)日：2007-01-04
申请号：PCT/US2005/039256
申请日：2005-10-31
申请人： APPLERA CORPORATION , LAU, Aldrich, N., K.
发明人： LAU, Aldrich, N., K.
IPC分类号： B01L3/00 , B03C5/02 , G01N33/543
摘要： The present teachings provide a manipulation chamber (10) , device, and method related to surface modifiers (80) added to an electrode (60) exposed to the biomolecules or a layer (70) adjacent to an electrode exposed to the biomolecules to decrease non-specific adsorption of the biomolecules such as proteins or nucleic acids in a biological sample.

6. 发明申请

WO2010117456A9 COLUMN ENRICHMENT OF PCR BEADS COMPRISING TETHERED AMPLICONS 审中-公开
标题翻译：包含四核苷酸的PCR珠的柱增殖
公开(公告)号：WO2010117456A9
公开(公告)日：2011-03-31
申请号：PCT/US2010001052
申请日：2010-04-08
申请人： APPLIED BIOSYSTEMS LLC , LAU ALDRICH N K , KARGER ACHIM , KINNEY PATRICK , NURSE JAMES , KEVEL AFTAB , GREINER DOUGLAS , GJERSTAD CARMAN
发明人： LAU ALDRICH N K , KARGER ACHIM , KINNEY PATRICK , NURSE JAMES , KEVEL AFTAB , GREINER DOUGLAS , GJERSTAD CARMAN
IPC分类号： C12Q1/68 , C12M1/34 , G01N30/60 , G01N33/48
CPC分类号： B01F3/0807 , B01F7/161 , B01F7/22 , B01F2215/0422 , B01F2215/0431 , B01F2215/0481

7. 发明申请

WO2010009426A3 DEVICES AND METHODS FOR REAGENT DELIVERY 审中-公开
标题翻译：用于试剂递送的装置和方法
公开(公告)号：WO2010009426A3
公开(公告)日：2010-04-01
申请号：PCT/US2009051041
申请日：2009-07-17
申请人： LIFE TECHNOLOGIES CORP , LEE LINDA G , WOO SAM L , COX DAVID M , LAU ALDRICH N K , REEL RICHARD T , VATTA PAOLO , YUE MIN
发明人： LEE LINDA G , WOO SAM L , COX DAVID M , LAU ALDRICH N K , REEL RICHARD T , VATTA PAOLO , YUE MIN
IPC分类号： C12M1/38 , C12Q1/68
CPC分类号： B01L3/0293 , B01L7/52 , B01L2400/0442 , B01L2400/0478 , B01L2400/0481 , G01N35/1002
摘要： A method for delivering reagent during a thermal cycling reaction may include placing a delivery device containing a reagent proximate to a reaction site, initiating a first stage of the reaction, wherein the first stage includes a period of an elevated temperature, and after completing the first stage of the reaction, dispensing the reagent from the delivery device to the reaction site, wherein the dispensing includes automatically actuating the delivery device to deliver the reagent.
摘要翻译：用于在热循环反应期间递送试剂的方法可以包括将包含试剂的递送装置放置在靠近反应位点的位置，引发反应的第一阶段，其中第一阶段包括升高的温度，并且在完成第一反应阶段将试剂从递送装置分配到反应部位，其中分配包括自动致动递送装置以输送试剂。

8. 发明申请

WO2010014820A3 PARTICLES FOR USE IN SUPPORTED NUCLEIC ACID LIGATION AND DETECTION SEQUENCING 审中-公开
标题翻译：用于支持核酸结合和检测顺序的颗粒
公开(公告)号：WO2010014820A3
公开(公告)日：2010-05-06
申请号：PCT/US2009052270
申请日：2009-07-30
申请人： LIFE TECHNOLOGIES CORP , LAU ALDRICH N K , OLDHAM MARK F
发明人： LAU ALDRICH N K , OLDHAM MARK F
IPC分类号： C12Q1/68 , C12Q3/00 , G01N33/50
CPC分类号： G01N33/54393 , C12Q1/6874
摘要： Compositions and methods to modify the surface of particles to which biomolecules are attached are disclosed. The particles can include beads and nanoparticles which are composed of metallics, metal alloys, glass, polymers and derivatives and composites thereof. The surface of the particles are modified to be hydrophilic for ease in the attachment of biomolecules to the particle surface and immobilization of the particles to a substrate to facilitate process such as nucleic acid sequencing, PCR and sequencing by ligation.
摘要翻译：公开了改变生物分子附着的颗粒表面的组合物和方法。颗粒可以包括由金属，金属合金，玻璃，聚合物及其衍生物及其复合材料组成的珠粒和纳米颗粒。将颗粒的表面改性为亲水的，以便于将生物分子附着到颗粒表面，并将颗粒固定到底物以促进诸如核酸测序，PCR和连接测序之类的过程。

9. 发明申请

WO2009009408A3 DEVICES AND METHODS FOR THE DETECTION OF ANALYTES 审中-公开
标题翻译：用于检测分析仪的装置和方法
公开(公告)号：WO2009009408A3
公开(公告)日：2009-04-16
申请号：PCT/US2008069126
申请日：2008-07-03
申请人： APPLIED BIOSYSTEMS , EASON ROBERT G , LAU ALDRICH N K , SCABOO KRISTIAN M , LI HANDONG
发明人： EASON ROBERT G , LAU ALDRICH N K , SCABOO KRISTIAN M , LI HANDONG
IPC分类号： G01N33/58
CPC分类号： G01N33/54366 , B01L3/5027 , B01L3/502761 , B01L2200/0647 , B01L2200/0668 , B01L2300/0636 , B01L2300/069 , B01L2300/0816 , B01L2300/0864 , G01N33/54326 , G01N2400/00
摘要： System and methods for detecting analytes such as pathogenic cells are described. The methods allow for the direct measurement of analytes such as pathogenic organisms without the need for sample preparation and/or PCR. The devices can be used individually as point-of-use sensors for airborne pathogens and other pathogenic organisms in foods and agriculture products.
摘要翻译：描述了用于检测分析物如致病细胞的系统和方法。这些方法允许直接测量分析物如病原体，而不需要样品制备和/或PCR。这些装置可以单独用作食品和农产品中的空气传播病原体和其他致病生物的使用点传感器。

10. 发明申请

WO2007050143A2 SURFACE MODIFICATION IN A MANIPULATION CHAMBER 审中-公开
标题翻译：操纵室中的表面修饰
公开(公告)号：WO2007050143A2
公开(公告)日：2007-05-03
申请号：PCT/US2006/022959
申请日：2006-06-13
申请人： APPLERA CORPORATION , LAU, Aldrich, N., K. , PHAN, Huan, L.
发明人： LAU, Aldrich, N., K. , PHAN, Huan, L.
IPC分类号： C07K1/26 , G01N27/00
CPC分类号： G01N27/305 , B03C11/00 , C07K1/26
摘要： A device for manipulating biological material, the device including at least one electrode and a photoconductive material configured to receive the biological material; and a light source configured to illuminate the photoconductive material so as to modulate an electric field, wherein the electric field is configured to manipulate the biological material; wherein a surface of the at least one electrode and/or the photoconductive material is modified with at least one of a carboxylic moiety, an amino moiety, a poly(ethylene glycol) moiety, a polymer of (poly(ethylene oxide) methyl ether) acrylate, a poly(2-hydroxyethyl (meth)acrylate), a poly(N-vinylpyrrolidone), a poly(N-vinylformamide), a poly(N-vinylformamide) derivative, a poly((meth)acrylamide), and a poly((meth)acrylamide) derivative. Methods for manipulating biological material are also disclosed.
摘要翻译：一种用于操纵生物材料的装置，该装置包括至少一个电极和被配置为容纳生物材料的光导材料; 以及光源，被配置为照亮所述光电导材料以调制电场，其中所述电场被配置为操纵所述生物材料; 其中所述至少一个电极和/或光电导材料的表面用羧基部分，氨基部分，聚（乙二醇）部分，（聚（环氧乙烷）甲基醚）的聚合物，丙烯酸酯，聚（甲基）丙烯酸2-羟乙酯），聚（N-乙烯基吡咯烷酮），聚（N-乙烯基甲酰胺），聚（N-乙烯基甲酰胺）衍生物，聚（（甲基）丙烯酰胺）聚（（甲基）丙烯酰胺）衍生物。还公开了用于操作生物材料的方法。

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