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1. 发明申请

WO2004058052A8 GENETIC POLYMORPHISMS ASSOCIATED WITH MYOCARDIAL INFARCTION, METHODS OF DETECTION AND USES THEREOF 审中-公开
标题翻译：与心肌梗塞相关的遗传多态性，检测方法及其用途
公开(公告)号：WO2004058052A8
公开(公告)日：2011-11-10
申请号：PCT/US0340978
申请日：2003-12-22
申请人： APPLERA CORP , CARGILL MICHELE , DEVLIN JAMES J , IAKOUBOVA OLGA
发明人： CARGILL MICHELE , DEVLIN JAMES J , IAKOUBOVA OLGA
IPC分类号： C12Q1/68 , A61B20060101 , C07H21/00 , C07H21/04 , C07K14/435 , C07K14/47 , C07K16/00 , C07K16/18 , C12N15/12 , C12P19/34 , C12Q1/44 , C12Q1/48 , G01N33/53
CPC分类号： C12Q1/6883 , C12Q2600/156
摘要： The present invention is based on the discovery of genetic polymorphisms that are associated with myocardial infarction. In particular, the present invention relates to nucleic acid molecules containing the polymorphisms, variant proteins encoded by such nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and proteins, and methods of using the nucleic acid and proteins as well as methods of using reagents for their detection. Figure 1 provides a diagrammatic representation of a computer-based discovery system containing the SNP information of the present invention in computer readable form.
摘要翻译：本发明基于与心肌梗塞相关的遗传多态性的发现。特别地，本发明涉及含有多态性的核酸分子，由该核酸分子编码的变体蛋白，用于检测多态核酸分子和蛋白质的试剂，以及使用该核酸和蛋白质的方法以及使用的方法用于检测的试剂。图1提供了以计算机可读形式包含本发明的SNP信息的基于计算机的发现系统的示意图。

2. 发明申请

WO2008144288A8 APPARATUS AND METHODS FOR PREPARATION OF SUBTANTIALLY UNIFORM EMULSIONS CONTAINING A PARTICLE 审中-公开
标题翻译：用于制备含有颗粒的亚基均匀乳液的装置和方法
公开(公告)号：WO2008144288A8
公开(公告)日：2009-06-04
申请号：PCT/US2008063480
申请日：2008-06-06
申请人： APPLERA CORP , LAU ALDRICH N K , CASSEL JONATHAN M , BRIDGHAM JOHN A
发明人： LAU ALDRICH N K , CASSEL JONATHAN M , BRIDGHAM JOHN A
IPC分类号： A61K8/06 , B01J13/00
CPC分类号： B01F3/0807 , B01F5/0475 , B01F5/0476 , B01F5/0485 , B01F13/0059
摘要： Methods and systems for forming water-in-oil emulsions are described. For example, an apparatus is described which includes: a first compartment containing a plurality of particles dispersed in an aqueous phase; a second compartment containing an oil phase; a porous layer separating the first and second compartments; and a device for applying pressure to the first compartment. A method is described which includes: moving an oil phase relative to a surface of a porous layer while simultaneously forcing an aqueous composition comprising particles through the porous layer and into the flowing dispersion medium thereby forming droplets of the aqueous composition containing particles dispersed in the oil phase. The aqueous composition can include one or more nucleic acid templates and reagents for amplifying the nucleic acids such as PCR reagents. A porous partition is described comprising a first and second major surfaces and at least two straight through pores comprising a cross sectional shape selected from a polygon, an oval, an oblong, a dumbbell, a bowtie and irregular shapes thereof. Aqueous droplets containing an oligonucleotide attached to a particle and reagents can be used as a microreactor for nucleic acid amplification.
摘要翻译：描述了形成油包水乳液的方法和系统。例如，描述了一种装置，其包括：包含分散在水相中的多个颗粒的第一隔室; 含有油相的第二隔室; 分离第一和第二隔室的多孔层; 以及用于向第一隔室施加压力的装置。描述了一种方法，其包括：相对于多孔层的表面移动油相，同时迫使包含颗粒的水性组合物通过多孔层并进入流动分散介质中，由此形成含有分散在油中的颗粒的含水组合物的液滴相。水性组合物可以包括一种或多种核酸模板和用于扩增核酸如PCR试剂的试剂。描述了一种多孔分隔件，其包括第一和第二主表面和至少两个直通孔，其包括选自多边形，椭圆形，长方形，哑铃，弓形和不规则形状的横截面形状。含有与粒子连接的寡核苷酸的水滴和试剂可以用作用于核酸扩增的微反应器。

3. 发明申请

WO2007050143A3 SURFACE MODIFICATION IN A MANIPULATION CHAMBER 审中-公开
标题翻译：操纵室中的表面修饰
公开(公告)号：WO2007050143A3
公开(公告)日：2009-05-14
申请号：PCT/US2006022959
申请日：2006-06-13
申请人： APPLERA CORP , LAU ALDRICH N K , PHAN HUAN L
发明人： LAU ALDRICH N K , PHAN HUAN L
IPC分类号： C12M1/34 , C12M3/00
CPC分类号： G01N27/305 , B03C11/00 , C07K1/26
摘要： A device for manipulating biological material, the device including at least one electrode and a photoconductive material configured to receive the biological material; and a light source configured to illuminate the photoconductive material so as to modulate an electric field, wherein the electric field is configured to manipulate the biological material; wherein a surface of the at least one electrode and/or the photoconductive material is modified with at least one of a carboxylic moiety, an amino moiety, a poly(ethylene glycol) moiety, a polymer of (poly(ethylene oxide) methyl ether) acrylate, a poly(2-hydroxyethyl (meth)acrylate), a poly(N-vinylpyrrolidone), a poly(N-vinylformamide), a poly(N-vinylformamide) derivative, a poly((meth)acrylamide), and a poly((meth)acrylamide) derivative. Methods for manipulating biological material are also disclosed.
摘要翻译：一种用于操纵生物材料的装置，该装置包括至少一个电极和被配置为容纳生物材料的光导材料; 以及光源，被配置为照亮所述光电导材料以调制电场，其中所述电场被配置为操纵所述生物材料; 其中所述至少一个电极和/或光电导材料的表面用羧基部分，氨基部分，聚（乙二醇）部分，（聚（环氧乙烷）甲基醚）的聚合物，丙烯酸酯，聚（甲基）丙烯酸2-羟乙酯），聚（N-乙烯基吡咯烷酮），聚（N-乙烯基甲酰胺），聚（N-乙烯基甲酰胺）衍生物，聚（（甲基）丙烯酰胺）聚（（甲基）丙烯酰胺）衍生物。还公开了用于操作生物材料的方法。

4. 发明申请

WO2007032817A3 PURE MIRNA SAMPLE PREPARATION METHOD 审中-公开
标题翻译：纯MIRNA样品制备方法
公开(公告)号：WO2007032817A3
公开(公告)日：2009-05-07
申请号：PCT/US2006027756
申请日：2006-07-17
申请人： APPLERA CORP , LAO KAI QIN
发明人： LAO KAI QIN
IPC分类号： C12Q1/68
CPC分类号： C12N15/1003 , C12N1/06 , C12N1/08 , C12N15/111 , C12N2310/14 , C12N2310/315 , C12N2310/321 , C12N2310/341 , C12N2310/346 , C12N2330/10 , C12N2330/30 , C12P19/34 , C12Q1/68 , C12N2310/3521
摘要： The present teachings provide novel methods, compositions, and kits for analyzing mature micro RNAs (miRNAs). By taking advantage of the observation that most mature miRNAs in cells are tightly associated with RISCs, the present teachings provide approaches for studying mature miRNAs without the complications of additional nucleic acids. For example, in some embodiments the present teachings provide a method of purifying mature miRNAs comprising heating a sample to form a lysate, and, degrading the additional nucleic acids. The resulting mixture lacks the additional nucleic acids, and contains mature miRNAs associated with RISCs. Liberating the mature miRNAs from RISCs, for example by a protease, a detergent, and/or heat, can result in a pure collection of mature miRNAs.
摘要翻译：本教导提供用于分析成熟微RNA（miRNA）的新型方法，组合物和试剂盒。通过利用细胞中大多数成熟miRNA与RISC紧密相关的观察，本教导提供了研究成熟miRNA而没有附加核酸并发症的方法。例如，在一些实施方案中，本发明提供纯化成熟miRNA的方法，其包括加热样品以形成裂解物，以及降解另外的核酸。所得混合物缺乏额外的核酸，并且含有与RISC相关的成熟miRNA。从RISC解离成熟的miRNA，例如通过蛋白酶，洗涤剂和/或加热，可导致成熟miRNA的纯收集。

5. 发明申请

WO2009014612A3 MODIFIED NUCLEOTIDES, METHODS FOR MAKING AND USING SAME 审中-公开
标题翻译：改性核子，制备和使用方法
公开(公告)号：WO2009014612A3
公开(公告)日：2009-04-30
申请号：PCT/US2008008613
申请日：2008-07-15
申请人： VISIGEN BIOTECHNOLOGIES INC , APPLERA CORP , WANG HONGYI , GAO XIAOLIAN , YU PEILIN , REDDY MITSU S , HARDIN SUSAN H , LINCECUM TOMMIE JR , WILLIAMS AMY , DELUGE NORHA , BELOSLUDTSEV YURI , MENCHEN STEVEN M , LAM JOE Y L , CHEN JER-KANG
发明人： WANG HONGYI , GAO XIAOLIAN , YU PEILIN , REDDY MITSU S , HARDIN SUSAN H , LINCECUM TOMMIE JR , WILLIAMS AMY , DELUGE NORHA , BELOSLUDTSEV YURI , MENCHEN STEVEN M , LAM JOE Y L , CHEN JER-KANG
IPC分类号： C07H21/00 , C12P19/30
CPC分类号： C07H21/04
摘要： Modified nucleotides are disclosed for use in single molecule sequencing, methods for making the modified nucleotides and method for using the modified nucleotides. Linkers for making the modified nucleotide are also disclosed.
摘要翻译：公开了用于单分子测序的修饰的核苷酸，用于制备修饰的核苷酸的方法和使用修饰的核苷酸的方法。还公开了用于制备修饰的核苷酸的接头。

6. 发明申请

WO2007024798A3 APPARATUS, SYSTEM, AND METHOD USING IMMISCIBLE-FLUID-DISCRETE-VOLUMES 审中-公开
标题翻译：装置，系统和使用可疑流体分离体积的方法
公开(公告)号：WO2007024798A3
公开(公告)日：2009-04-30
申请号：PCT/US2006032637
申请日：2006-08-22
申请人： APPLERA CORP , LEE LINDA G , WOO SAM L , MA CONGCONG , REEL RICHARD T , OLDHAM MARK F , COX DAVID M , SCHROEDER BENJAMIN G , SORENSON JON M , WIYATNO WILLY
发明人： LEE LINDA G , WOO SAM L , MA CONGCONG , REEL RICHARD T , OLDHAM MARK F , COX DAVID M , SCHROEDER BENJAMIN G , SORENSON JON M , WIYATNO WILLY
IPC分类号： C12P19/34
CPC分类号： C12Q1/6869 , B01J2219/00353 , B01J2219/0036 , B01J2219/00364 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00657 , B01J2219/00659 , B01L3/0293 , B01L3/502715 , B01L3/502784 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2300/0864 , B01L2300/0867 , B01L2400/0421 , B01L2400/0487 , C12Q1/6806 , C12Q1/6874 , F15C5/00 , F16K99/0001 , F16K99/0011 , F16K99/0013 , F16K2099/0084 , G01N1/14 , G01N27/44743 , G01N27/44769 , G01N35/08 , Y10T137/0318 , Y10T137/4259 , Y10T137/85978 , Y10T137/85986 , Y10T137/86863 , Y10T436/2575 , C12Q2535/101
摘要： Various embodiments of the teachings relate to a system or method for sample preparation or analysis in biochemical or molecular biology procedures. The sample preparation can involve small volume processed in discrete portions or segments or slugs, herein referred to as discrete volumes. A molecular biology procedure can be nucleic acid analysis. Nucleic acid analysis can be an integrated DNA amplification/DNA sequencing procedure.
摘要翻译：本发明的各种实施方案涉及生物化学或分子生物学程序中用于样品制备或分析的系统或方法。样品制备可以涉及在离散部分或段或块中的小体积处理，在本文中称为离散体积。分子生物学程序可以是核酸分析。核酸分析可以是一个综合的DNA扩增/ DNA测序程序。

7. 发明申请

WO2006102415A3 OPTICAL CAMERA ALIGNMENT 审中-公开
标题翻译：光学相机对准
公开(公告)号：WO2006102415A3
公开(公告)日：2009-04-16
申请号：PCT/US2006010409
申请日：2006-03-22
申请人： APPLERA CORP , BLASENHEIM BARRY J , KAO H PIN , OLDHAM MARK F
发明人： BLASENHEIM BARRY J , KAO H PIN , OLDHAM MARK F
IPC分类号： G02B7/02 , B01L3/00 , B01L7/00 , B01L9/00 , B01L99/00 , G01N21/25 , G01N21/27 , G01N21/64 , G01N35/00 , G01N35/02 , G01N35/04 , G01N35/10
CPC分类号： G01N21/253 , B01L3/0293 , B01L3/5025 , B01L3/5027 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L7/52 , B01L9/50 , B01L9/523 , B01L2200/021 , B01L2200/025 , B01L2200/0605 , B01L2200/0642 , B01L2200/0689 , B01L2200/142 , B01L2300/021 , B01L2300/022 , B01L2300/044 , B01L2300/046 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/1883 , B01L2400/0406 , B01L2400/0409 , B01L2400/0487 , G01N21/274 , G01N21/6452 , G01N21/6486 , G01N35/0099 , G01N35/028 , G01N35/1002 , G01N35/1074 , G01N2035/00287 , G01N2035/00366 , G01N2035/0405 , G01N2035/0425 , G01N2035/1039
摘要： A camera alignment system that can enable alignment in at least one of three planes and about an axis of at least one of the planes. An alignment mount can mate to a camera and lens. The alignment mount can comprise a mechanism to adjust the camera relative to the lens to that an image plane of the camera aligns with an image plane of the lens in a predetermined orientation. One predetermined orientation can be that the image plane of the camera being parallel to the image plane of the lens.
摘要翻译：一种相机对准系统，其能够实现三个平面中的至少一个和至少一个平面的轴线的对准。对准安装座可与摄像机和镜头配合使用。对准安装座可以包括一个机构，用于相机相对于镜头调整到照相机的图像平面与预定取向的透镜的图像平面对准。一个预定方向可以是相机的图像平面平行于透镜的图像平面。

8. 发明申请

WO2005077125A3 METHODS AND COMPOSITIONS FOR DETECTING NUCLEIC ACIDS 审中-公开
标题翻译：用于检测核酸的方法和组合物
公开(公告)号：WO2005077125A3
公开(公告)日：2009-03-19
申请号：PCT/US2005004662
申请日：2005-02-11
申请人： APPLERA CORP , SCHROEDER BENJAMIN G
发明人： SCHROEDER BENJAMIN G
IPC分类号： C12Q1/68 , C12N9/22
CPC分类号： C12Q1/6813
摘要： A reporter molecule for detecting a nucleic acid is disclosed. The molecule comprises an enzyme having a kcat of at least about 200 sec-1, a reversible inhibitor of the enzyme inhibitorily engaging the enzyme, and a nucleobase polymer extending between the enzyme and the inhibitor. The polymer interferes with the engagement between the inhibitor and the enzyme when the nucleic acid contacts the polymer. Methods of making and methods of using the reporter molecule are also disclosed.
摘要翻译：公开了用于检测核酸的报告分子。该分子包含具有至少约200秒-1的kcat的酶，抑制酶接合的酶的可逆抑制剂和在酶和抑制剂之间延伸的核碱基聚合物。当核酸与聚合物接触时，聚合物干扰抑制剂和酶之间的接合。还公开了制备方法和使用报道分子的方法。

9. 发明申请

WO2008005674A3 METHODS OF ANALYZING BINDING INTERACTIONS 审中-公开
标题翻译：分析结合相互作用的方法
公开(公告)号：WO2008005674A3
公开(公告)日：2008-12-24
申请号：PCT/US2007071270
申请日：2007-06-14
申请人： APPLERA CORP , SUN HONGYE , LIVAK KENNETH J
发明人： SUN HONGYE , LIVAK KENNETH J
IPC分类号： G01N33/53
CPC分类号： G01N33/566 , G01N33/48721
摘要： The present disclosure relates to methods of analyzing binding interactions between a binding component and a receptor component by translocating unbound and any bound components through a pore and detecting the unbound and bound components.
摘要翻译：本公开内容涉及通过将未结合的组分和任何结合的组分通过孔移位并检测未结合和结合的组分来分析结合组分和受体组分之间的结合相互作用的方法。

10. 发明申请

WO2008018855A3 METHODS AND KITS FOR EVALUATING DNA METHYLATION 审中-公开
标题翻译：评估DNA甲基化的方法和试剂盒
公开(公告)号：WO2008018855A3
公开(公告)日：2008-12-04
申请号：PCT/US2006023669
申请日：2006-06-16
申请人： APPLERA CORP , BOYD VICTORIA , LIVAK KENNETH , ZON GERALD
发明人： BOYD VICTORIA , LIVAK KENNETH , ZON GERALD
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2565/125 , C12Q2523/125
摘要： Methods and kits are disclosed for determining the degree of methylation of at least one target region. Typically a sample is exposed to a modifying agent to obtain a modified sample comprising a modified nucleotide. At least one target region in the modified sample is amplified. Some of the disclosed methods comprise at least one additional amplification reaction. In some embodiments, at least one mobility shifting analog is incorporated into an amplicon during an amplification reaction. The analogs are analyzed and the degree of methylation of at least one target region is determined.
摘要翻译：公开了用于确定至少一个靶区域的甲基化程度的方法和试剂盒。通常将样品暴露于修饰剂以获得包含修饰的核苷酸的修饰的样品。修改样本中的至少一个目标区域被扩增。一些公开的方法包含至少一个额外的扩增反应。在一些实施方案中，在扩增反应期间将至少一种迁移率偏移类似物并入扩增子中。分析类似物并确定至少一个靶区域的甲基化程度。

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