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1. 发明申请

WO2010117456A9 COLUMN ENRICHMENT OF PCR BEADS COMPRISING TETHERED AMPLICONS 审中-公开
标题翻译：包含四核苷酸的PCR珠的柱增殖
公开(公告)号：WO2010117456A9
公开(公告)日：2011-03-31
申请号：PCT/US2010001052
申请日：2010-04-08
申请人： APPLIED BIOSYSTEMS LLC , LAU ALDRICH N K , KARGER ACHIM , KINNEY PATRICK , NURSE JAMES , KEVEL AFTAB , GREINER DOUGLAS , GJERSTAD CARMAN
发明人： LAU ALDRICH N K , KARGER ACHIM , KINNEY PATRICK , NURSE JAMES , KEVEL AFTAB , GREINER DOUGLAS , GJERSTAD CARMAN
IPC分类号： C12Q1/68 , C12M1/34 , G01N30/60 , G01N33/48
CPC分类号： B01F3/0807 , B01F7/161 , B01F7/22 , B01F2215/0422 , B01F2215/0431 , B01F2215/0481

2. 发明申请

WO2010117461A2 SYSTEM AND METHOD FOR PREPARING AND USING BULK EMULSION 审中-公开
标题翻译：制备和使用大豆乳液的系统和方法
公开(公告)号：WO2010117461A2
公开(公告)日：2010-10-14
申请号：PCT/US2010001059
申请日：2010-04-08
申请人： APPLIED BIOSYSTEMS LLC , LAU ALDRICH N K , LAMBERT CHRISTINE , KARGER ACHIM , JORDAN MADISON
发明人： LAU ALDRICH N K , LAMBERT CHRISTINE , KARGER ACHIM , JORDAN MADISON
IPC分类号： C12Q1/68 , C12M1/02 , C12M1/38
CPC分类号： B01F3/0807 , B01F7/161 , B01F7/22 , B01F2215/0422 , B01F2215/0431 , B01F2215/0481
摘要： An emulsion generation apparatus and method for forming an emulsion are provided wherein a customized impeller design is adapted to form an emulsion with a desired droplet size that defines a desired volume. The emulsion generation apparatus provides improved uniformity in emulsion preparation and may be used to create large or small volume emulsions rapidly and reproducibly. A system and method are also provided for large volume sample amplification adaptable for use with conventional PCR-based reactions as well as emulsion-based PCR reactions and other reactions. For applications involving emulsion- based PCR amplification, the system and method provide improved uniformity in emulsion amplification and can be used to amplify large or small volume emulsions rapidly and reproducibly.
摘要翻译：提供了一种用于形成乳液的乳液生成装置和方法，其中定制的叶轮设计适于形成具有限定期望体积的所需液滴尺寸的乳液。乳液生成装置提供乳液制备中改进的均匀性，并可用于快速且可再现地产生大体积或小体积的乳液。还提供了适用于常规基于PCR的反应以及基于乳液的PCR反应和其它反应的大体积样品扩增的系统和方法。对于涉及基于乳液的PCR扩增的应用，该系统和方法提供了乳液扩增的改进的均匀性，并可用于快速和可重复地扩增大体积或小体积的乳液。

3. 发明申请

WO2011143194A3 SYSTEM AND METHOD FOR PROCESSING A BIOLOGICAL SAMPLE 审中-公开
标题翻译：用于处理生物样品的系统和方法
公开(公告)号：WO2011143194A3
公开(公告)日：2012-04-19
申请号：PCT/US2011035894
申请日：2011-05-10
申请人： LIFE TECHNOLOGIES CORP , SONG MAENGSEOK , KARGER ACHIM , KINNEY PATRICK
发明人： SONG MAENGSEOK , KARGER ACHIM , KINNEY PATRICK
IPC分类号： C12Q1/68 , C12M1/00 , C12N15/10 , G01N33/48
CPC分类号： B01L3/502761 , B01F5/0652 , B01F2003/0823 , B01L7/52 , B01L2200/0673 , B01L2400/0439 , C12Q1/6806
摘要： Systems and methods for processing a biological sample are provided herein. For example, the system can be configured to deaggregate/declump a sample before, during, and/or after sample preparation and/or sample analysis. For example, the system can include a deaggregation device/system in communication with, for example, a nucleic acid amplification process (e.g., an ePCR system). Various embodiments of the deaggregation device are provided herein. For example, in some embodiments, the deaggregation device can include a valve, a valve manifold, a conduit, a channel, or some combinations thereof.
摘要翻译：本文提供了用于处理生物样品的系统和方法。例如，可以将系统配置为在样品制备和/或样品分析之前，期间和/或之后对样品进行解聚/递归。例如，该系统可以包括与例如核酸扩增过程（例如，ePCR系统）通信的解聚集装置/系统。本文提供了解聚集装置的各种实施例。例如，在一些实施例中，解聚装置可以包括阀，阀歧管，导管，通道或其一些组合。

4. 发明申请

WO2005118862A3 COMPOSITIONS, METHODS, AND KITS FOR (MIS)LIGATING OLIGONUCLEOTIDES 审中-公开
标题翻译：（MIS）发光二极管的组成，方法和工具
公开(公告)号：WO2005118862A3
公开(公告)日：2006-07-13
申请号：PCT/US2005014975
申请日：2005-04-29
申请人： APPLERA CORP , KARGER ACHIM , ROZZELLE JAMES , CHANG CHIEN-WEI , BOLCHAKOVA ELENA
发明人： KARGER ACHIM , ROZZELLE JAMES , CHANG CHIEN-WEI , BOLCHAKOVA ELENA
IPC分类号： C12N9/00 , C12N9/22 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6844 , C12N9/93 , C12Q1/6858 , C12Q1/6862 , C12Q2537/143 , C12Q2521/501
摘要： Methods, reagents, and kits for (mis)ligating oligonucleotide probes or for identifying at least one target nucleotide are disclosed. One can enhance the generation of misligation product using a ligase under reaction conditions and with reagents where that particular ligase is prone to misligation. Alternatively, one can decrease or avoid generating misligation products using a particular ligase under reaction conditions and using reagents where that ligase is at least less prone to misligation. In certain embodiments, the recombinant ligase from Archaeoglobus fulgidus (Afu) is employed due to its unique misligation properties.
摘要翻译：公开了用于（错误）连接寡核苷酸探针或用于鉴定至少一种靶核苷酸的方法，试剂和试剂盒。可以在反应条件下使用连接酶和使用特异性连接酶容易发生错误的试剂来增强产生错误产物。或者，可以减少或避免在反应条件下使用特定的连接酶产生错误产物，并使用试剂，其中该连接酶至少不易于误引。在某些实施方案中，由于其独特的错误性质，使用来自古细菌（Afu）的重组连接酶。

5. 发明申请

WO2006076017A3 METHODS AND KITS FOR IDENTIFYING TARGET NUCLEOTIDES IN MIXED POPULATIONS 审中-公开
标题翻译：识别混合人口中目标核素的方法和工具
公开(公告)号：WO2006076017A3
公开(公告)日：2007-05-31
申请号：PCT/US2005015027
申请日：2005-04-29
申请人： APPLERA CORP , KARGER ACHIM , BOLCHAKOVA ELENA
发明人： KARGER ACHIM , BOLCHAKOVA ELENA
IPC分类号： C12N9/00 , C12Q1/68
CPC分类号： C12Q1/68 , C12Q1/6827 , C12Q1/6862 , C12Q2521/501 , C12Q2565/301
摘要： Ligation-based methods and kits are disclosed for identifying at least two target nucleotides in a mixed population sample, that is a sample that contains or potentially contains target nucleic acid sequences from more than one source. Typically, two ligation reaction compositions are formed, ligation products generated, and the ligation products or their surrogates are analyzed to identify target nucleotides in the mixed population sample. In certain embodiments, the target nucleic acid sequences, the ligation products, or both are amplified. In certain embodiments, multiplex amplification and/or ligation reactions are performed.
摘要翻译：公开了基于连接的方法和试剂盒，用于鉴定混合群体样品中的至少两个靶核苷酸，即来自多于一个来源的含有或潜在地含有靶核酸序列的样品。通常，形成两个连接反应组合物，产生连接产物，并分析连接产物或其替代物以鉴定混合群体样品中的靶核苷酸。在某些实施方案中，扩增靶核酸序列，连接产物或两者。在某些实施方案中，进行多重扩增和/或连接反应。

6. 发明申请

WO2007095493A3 SYSTEMS AND METHODS FOR METHYLATION PREDICTION 审中-公开
标题翻译：用于甲基化预测的系统和方法
公开(公告)号：WO2007095493A3
公开(公告)日：2007-10-04
申请号：PCT/US2007061982
申请日：2007-02-12
申请人： APPLERA CORP , KARGER ACHIM , BOYD VICKI
发明人： KARGER ACHIM , BOYD VICKI
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , Y10T436/143333 , C12Q2565/125 , C12Q2545/114 , C12Q2523/125
摘要： A method is provided for predicting an amount of methylation of at least one target region. The method includes establishing an observed size of a plurality of oligonucleotides relative to a size standard and correlating the observed size to a number of each of nucleotide base in each of the plurality of oligonucleotides. A mobility coefficient can be determined for each base of each respective oligonucleotide and the determined mobility coefficients can be applied to a predetermined number of polynucleotides subjected to methylation detection analysis. The plurality of oligonucleotides are treated with a modifying agent to obtain amplicons in methylated and unmethylated target regions and amplicons derived from methylated and unmethylated target regions are distinguished based on their relative mobilities. The degree of methylation can be predicted based on distinguished methylated regions.
摘要翻译：提供了用于预测至少一个目标区域的甲基化量的方法。该方法包括相对于大小标准建立多个寡核苷酸的观察大小，并将观察到的大小与多个寡核苷酸中的每一个中的每个核苷酸碱基的数量相关联。可以针对每个相应的寡核苷酸的每个碱基确定迁移系数，并且可以将确定的迁移系数应用于进行甲基化检测分析的预定数量的多核苷酸。用修饰剂处理多个寡核苷酸以获得甲基化和非甲基化靶区域中的扩增子，并且基于其相对迁移率区分源自甲基化和未甲基化靶标区域的扩增子。甲基化程度可根据区分的甲基化区域预测。

7. 发明申请

WO2006119419A2 MATERIALS AND KITS FOR USE IN HOT-START PCR, AND METHODS OF AMPLIFYING NUCLEIC ACIDS IN A POLYMERASE CHAIN REACTION 审中-公开
标题翻译：在热启动PCR中使用的材料和试剂盒以及聚合酶链反应中核酸的放大方法
公开(公告)号：WO2006119419A2
公开(公告)日：2006-11-09
申请号：PCT/US2006017133
申请日：2006-05-03
申请人： JEON GEUNSOOK , VANN CHARLES , KARGER ACHIM , LAU ALDRICH , KHAN SHAHEER
发明人： JEON GEUNSOOK , VANN CHARLES , KARGER ACHIM , LAU ALDRICH , KHAN SHAHEER
CPC分类号： C12Q1/6848 , C12Q1/6806 , C12Q1/686 , C12Q2549/101 , C12Q2527/125 , C12Q2547/107
摘要： Materials for sequestering reagents in hot-start PCR, kits containing such materials, and methods for the use of such materials in amplifying nucleic acids are described.
摘要翻译：描述了在热启动PCR中螯合试剂的材料，含有这些材料的试剂盒，以及在扩增核酸中使用这些材料的方法。

8. 发明申请

WO2006102352A2 NORMALIZATION OF DATA USING CONTROLS 审中-公开
标题翻译：使用控制数据的正规化
公开(公告)号：WO2006102352A2
公开(公告)日：2006-09-28
申请号：PCT/US2006010291
申请日：2006-03-21
申请人： APPLERA CORP , KAO H PIN , HARDING IAN A , KARGER ACHIM , OLDHAM MARK F , OSTADAN OMEAD , YOUNG GREG , HAYASHIBARA KATHLEEN C , LAIRD HYLAND FIONA C , PRATT MARK R , STEVENS JUNCO F , TOMANEY AUSTIN B
发明人： KAO H PIN , HARDING IAN A , KARGER ACHIM , OLDHAM MARK F , OSTADAN OMEAD , YOUNG GREG , HAYASHIBARA KATHLEEN C , LAIRD HYLAND FIONA C , PRATT MARK R , STEVENS JUNCO F , TOMANEY AUSTIN B
IPC分类号： C12Q1/68 , G01N33/48 , G01N33/50 , G06F19/00
CPC分类号： C12Q1/6806
摘要： A method of using a standard to correct for variability in sample handling, can comprise (a) adding a template of known concentration to an assay comprising a sample; (b) preamplifying the assay; (c) amplifying the assay; (d) collecting data during the amplifying; and (e) correcting the data using a comparison of data collected from the template to data collected from the sample.
摘要翻译：使用标准来校正样品处理的变异性的方法可以包括（a）将已知浓度的模板加入到包含样品的测定中; （b）预扩增测定; （c）扩增测定; （d）放大期间收集数据; 和（e）使用从模板收集的数据与从样本收集的数据的比较来校正数据。

9. 发明申请

WO2006005055A3 METHODS REACTION MIXTURES AND KITS FOR LIGATING POLYNUCLEOTIDES 审中-公开
标题翻译：方法反应混合物和用于引发多核苷酸的试剂盒
公开(公告)号：WO2006005055A3
公开(公告)日：2006-03-16
申请号：PCT/US2005023737
申请日：2005-06-30
申请人： APPLERA CORP , ANDERSEN MARK , WENZ MICHAEL H , CHEN CAIFU , KARGER ACHIM
发明人： ANDERSEN MARK , WENZ MICHAEL H , CHEN CAIFU , KARGER ACHIM
IPC分类号： C12Q1/48 , C12Q1/527 , C12Q1/68
CPC分类号： C12Q1/6855 , C12P19/34
摘要： The present teachings pertain to methods, reaction mixtures, and kits for ligating polynucleotides. In some embodiments, a heat-activatable ligation agent, a phosphorylation agent, and a decontamination agent are included in the same ligation reaction mixture with at least one probe set, at least one linker set, and at least one target polynucleotide. A reaction at a first temperature results in hybridization of the probes to the target, phosphorylation of the probes, and decontamination of unwanted reaction components. A reaction at a second temperature results in the ligation of the probes together. In some embodiments, the present teachings are applied in highly multi-plexed ligation reactions in which a plurality of single nucleotide polymorphisms in a plurality of target polynucleotides are queried, and eventually detected using a mobility dependent analysis technique.
摘要翻译：本教导涉及用于连接多核苷酸的方法，反应混合物和试剂盒。在一些实施方案中，与至少一个探针组，至少一个接头组和至少一个靶多核苷酸在相同的连接反应混合物中包括可热激活的连接剂，磷酸化剂和去污剂。在第一温度下的反应导致探针与靶的杂交，探针的磷酸化，以及不期望的反应成分的去污。在第二温度下的反应导致探头连接在一起。在一些实施方案中，本教导应用于高多重连接的连接反应中，其中查询多个目标多核苷酸中的多个单核苷酸多态性，并且最终使用迁移率依赖性分析技术检测。

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