专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2007124186A3 EXPRESSION CONTROL ELEMENTS FROM THE LEMNACEAE FAMILY 审中-公开
标题翻译：来自LEMNACEAE FAMILY的表达控制元素
公开(公告)号：WO2007124186A3
公开(公告)日：2008-04-24
申请号：PCT/US2007060614
申请日：2007-01-17
申请人： BIOLEX THERAPEUTICS INC , DICKEY LYNN F , COX KEVIN M , PEELE CHARLES G
发明人： DICKEY LYNN F , COX KEVIN M , PEELE CHARLES G
IPC分类号： C07K14/415 , A01H5/00 , C12N15/82
CPC分类号： C12N15/8216 , C07K14/415 , C12N9/2442 , C12N9/88 , C12N15/8221 , C12N15/8222 , C12N15/8223 , C12Y302/01014 , C12Y401/01039
摘要： Compositions and methods for regulating expression of nucleotide sequences of interest in a plant are provided. Compositions include novel nucleic acid molecules, and variants and fragments thereof, for expression control elements isolated from the Lemnaceae ubiquitin, r-histone and chinatase genes. A method for expressing a nucleotide sequence of interest in a plant using the expression control elements disclosed herein is further provided. Also provided is a novel Lemnaceae signal peptide-encoding sequence and the signal peptide encoded thereby. Where an expression construct of the invention is designed to express a polypeptide of interest, this novel signal peptide-encoding sequence can be included within the expression construct of the invention to provide for extracellular secretion of the encoded polypeptide of interest.
摘要翻译：提供了用于调节植物中感兴趣的核苷酸序列的表达的组合物和方法。组合物包括新的核酸分子及其变体和片段，用于从莲子科泛素，r-组蛋白和chinatase基因分离的表达控制元件。还提供了使用本文公开的表达控制元件在植物中表达目标核苷酸序列的方法。还提供了一种新颖的莲子科信号肽编码序列和由其编码的信号肽。当本发明的表达构建体设计用于表达感兴趣的多肽时，该新型信号肽编码序列可以包括在本发明的表达构建体内，以提供所编码的目标多肽的细胞外分泌。

2. 发明申请

WO2007084672A2 MONOCLONAL ANTIBODIES AGAINST CD30 LACKING IN FUCOSYL AND XYLOSYL RESIDUES 审中-公开
标题翻译：单克隆抗体对抗CD30在纤维素和木糖残留物中的缺乏
公开(公告)号：WO2007084672A2
公开(公告)日：2007-07-26
申请号：PCT/US2007001451
申请日：2007-01-17
申请人： MEDAREX INC , BLACK AMELIA NANCY , PASSMORE DAVID B , SRINIVASAN MOHAN , DICKEY LYNN F , COX KEVIN M , PEELE CHARLES G , WANG MING-BO
发明人： BLACK AMELIA NANCY , PASSMORE DAVID B , SRINIVASAN MOHAN , DICKEY LYNN F , COX KEVIN M , PEELE CHARLES G , WANG MING-BO
IPC分类号： C07K16/00 , A61K39/395 , A61P35/00 , A61P37/00 , C07K16/28
CPC分类号： C07K16/2878 , A61K2039/505 , C07K2317/13 , C07K2317/21 , C07K2317/41 , C07K2317/72 , C07K2317/732
摘要： The invention pertains to anti-CD30 antibodies that lack fucosyl and xylosyl residues. The antibodies of the invention exhibit increased antibody-dependent cellular cytotoxicity (ADCC) activity, including the ability to Iyse CD30-expressing cell lines that are not lysed by the fucosylated and xylosylated form of the antibodies. The invention also provides host cells that express the anti-CD30 antibodies that lack fucosyl and xylosyl residues, wherein the host cells are deficient for a fucosyltransferase and a xylosyltransferase. Methods of using the antibodies to inhibit the growth of CD30 + cells, such as tumor cells, are also provided.
摘要翻译：本发明涉及缺乏岩藻糖基和木糖基残基的抗CD30抗体。本发明的抗体表现出增加的抗体依赖性细胞毒性（ADCC）活性，包括能够表达抗原的岩藻糖基化和木糖基化形式不裂解的表达CD30的细胞系。本发明还提供了表达缺乏岩藻糖基和木糖基残基的抗CD30抗体的宿主细胞，其中宿主细胞对于岩藻糖基转移酶和木糖基转移酶是不足的。还提供了使用抗体抑制CD30 + / +细胞如肿瘤细胞生长的方法。

3. 发明申请

WO2005078109A2 EXPRESSION OF PLASMINOGEN AND MICROPLASMINOGEN IN DUCKWEED 审中-公开
标题翻译：表达胰岛素和微管蛋白在DUCKWEED中的表达
公开(公告)号：WO2005078109A2
公开(公告)日：2005-08-25
申请号：PCT/US2005/004245
申请日：2005-02-11
申请人： BIOLEX, INC. , SPENCER, David , DICKEY, Lynn, F. , GASDASKA, John, R. , WANG, Xiaowei , COX, Kevin, M. , PEELE, Charles, G.
发明人： SPENCER, David , DICKEY, Lynn, F. , GASDASKA, John, R. , WANG, Xiaowei , COX, Kevin, M. , PEELE, Charles, G.
IPC分类号： C12N15/82
CPC分类号： C12N15/8257 , C12N9/6435 , C12Y304/21007
摘要： The present invention provides methods and compositions for the production of recombinant plasminogen, microplasminogen, and fragments thereof in a duckweed expression system. It is the novel finding of the present invention that a duckweed expression system may be used to produce high levels of plasminogen and microplasminogen. The duckweed-produced plasminogen and microplasminogen can be activated to produce a polypeptide having protease activity. Thus, the invention encompasses methods for the expression of plasminogen, microplasminogen, and fragments thereof in duckweed, duckweed plants that are transformed with expression cassettes for the expression of plasminogen, microplasminogen, and fragments thereof, and nucleic acids comprising nucleotide sequences encoding plasminogen, microplasminogen, and fragments thereof, where these nucleotide sequences are modified to enhance their expression in duckweed.
摘要翻译：本发明提供了用于在浮萍表达系统中生产重组纤溶酶原，微量血浆原和其片段的方法和组合物。本发明的新发现是，浮萍表达系统可用于产生高水平的纤溶酶原和微量淀粉样蛋白。可以将浮萍产生的纤溶酶原和微量血浆原活化以产生具有蛋白酶活性的多肽。因此，本发明包括在用于表达纤溶酶原，微量血浆原和其片段的表达盒转化的浮萍，浮萍植物中表达纤溶酶原，微量淀粉样蛋白及其片段的方法，以及包含编码纤溶酶原，微量稀释细胞的核苷酸序列的核酸及其片段，其中这些核苷酸序列被修饰以增强其在浮萍中的表达。

4. 发明申请

WO0234156A3 FLOATING BEARING KNEE JOINT PROSTHESIS WITH A FIXED TIBIAL POST 审中-公开
标题翻译：浮动轴承KNEE接头与固定的TIBIAL POST
公开(公告)号：WO0234156A3
公开(公告)日：2003-01-23
申请号：PCT/US0145264
申请日：2001-10-24
申请人： BIOMET INC , METZGER ROBERT , BROWN DAVID RAY , HERSHBERGER TROY , COX KEVIN
发明人： METZGER ROBERT , BROWN DAVID RAY , HERSHBERGER TROY , COX KEVIN
IPC分类号： A61F2/00 , A61F2/30 , A61F2/38 , A61F2/64
CPC分类号： A61F2/3868 , A61F2/3859 , A61F2/3886 , A61F2/389 , A61F2002/3009 , A61F2002/30093 , A61F2002/30133 , A61F2002/30153 , A61F2002/30332 , A61F2002/30448 , A61F2002/30561 , A61F2002/30589 , A61F2002/30604 , A61F2002/30682 , A61F2002/30878 , A61F2210/0019 , A61F2220/0033 , A61F2220/005 , A61F2230/0015 , A61F2230/0019 , A61F2250/0091 , A61F2310/00023 , A61F2310/00029 , A61F2310/00353
摘要： This invention relates to a floating bearing knee joint prosthesis (146) having a fixed tibial post used for replacing the articulating knee portion of a femur and a tibia. The floating bearing knee joint prosthesis (146) includes a femoral component (152), a tibial component (154), a guide post (206) and a bearing member (156). The femoral component (152) includes an engagement member (176), a first femoral bearing surface (162) and a second femoral bearing surface (164). The tibial component (154) includes a tibial bearing surface (196). The guide post (206) extends from the tibial component (154) and is operable to be engaged by the engagement member (176) of the femoral component (152). The bearing member (156) includes a first bearing surface (200) that articulates with the first femoral bearing surface (162), a second bearing surface (202) that articulates with the first femoral bearing surface (164) and a third bearing surface (198) that articulates with the tibial bearing surface (196). The bearing member (156) further includes a posterior lip extension (284) that substantially inhibits the bearing member (156) from dislocating upon flexion of the knee prosthesis (146).
摘要翻译：本发明涉及一种具有固定的胫骨柱的浮动轴承膝关节假体（146），用于替换股骨和胫骨的关节膝盖部分。浮动轴承膝关节假体（146）包括股骨部件（152），胫骨部件（154），引导柱（206）和轴承部件（156）。股骨部件（152）包括接合构件（176），第一股骨支承表面（162）和第二股骨支承表面（164）。胫骨部件（154）包括胫骨支承表面（196）。引导柱（206）从胫骨部件（154）延伸并且可操作以由股骨部件（152）的接合部件（176）接合。轴承构件（156）包括与第一股骨承载表面（162）铰接的第一支承表面（200），与第一股骨承载表面（164）铰接的第二支承表面（202）和第三支承表面 198），其与胫骨支承表面（196）铰接。轴承构件（156）还包括后唇延伸部（284），其基本上阻止所述支承构件（156）在膝盖假体（146）弯曲时脱位。

5. 发明申请

WO2007146857A8 ANTI- MCP-1 ANTIBODIES EXPRESSED IN LEMNA, COMPOSITIONS, METHODS AND USES 审中-公开
标题翻译： LEMNA中表达的抗MCP-1抗体，组合物，方法和用途
公开(公告)号：WO2007146857A8
公开(公告)日：2008-12-18
申请号：PCT/US2007070833
申请日：2007-06-11
申请人： CENTOCOR INC , DAS ANUK , SWEET RAYMOND , TSUI PING , BARDROFF MICHAEL , LUO JINQUAN , COX KEVIN M
发明人： DAS ANUK , SWEET RAYMOND , TSUI PING , BARDROFF MICHAEL , LUO JINQUAN , COX KEVIN M
IPC分类号： A61K39/395 , C07H21/04 , C07K16/18 , C12N5/00 , C12N15/00 , C12P21/00 , C12P21/08
CPC分类号： C07K16/24 , A61K2039/505 , C07K2317/13

6. 发明申请

WO2005078109A3 EXPRESSION OF PLASMINOGEN AND MICROPLASMINOGEN IN DUCKWEED 审中-公开
标题翻译：杜氏鲍曼不动杆菌的表达及其微丝体表达
公开(公告)号：WO2005078109A3
公开(公告)日：2005-11-10
申请号：PCT/US2005004245
申请日：2005-02-11
申请人： BIOLEX INC , SPENCER DAVID , DICKEY LYNN F , GASDASKA JOHN R , WANG XIAOWEI , COX KEVIN M , PEELE CHARLES G
发明人： SPENCER DAVID , DICKEY LYNN F , GASDASKA JOHN R , WANG XIAOWEI , COX KEVIN M , PEELE CHARLES G
IPC分类号： A01H1/00 , C12N9/68 , C12N15/82
CPC分类号： C12N15/8257 , C12N9/6435 , C12Y304/21007
摘要： The present invention provides methods and compositions for the production of recombinant plasminogen, microplasminogen, and fragments thereof in a duckweed expression system. It is the novel finding of the present invention that a duckweed expression system may be used to produce high levels of plasminogen and microplasminogen. The duckweed-produced plasminogen and microplasminogen can be activated to produce a polypeptide having protease activity. Thus, the invention encompasses methods for the expression of plasminogen, microplasminogen, and fragments thereof in duckweed, duckweed plants that are transformed with expression cassettes for the expression of plasminogen, microplasminogen, and fragments thereof, and nucleic acids comprising nucleotide sequences encoding plasminogen, microplasminogen, and fragments thereof, where these nucleotide sequences are modified to enhance their expression in duckweed.
摘要翻译：本发明提供了用于在浮萍表达系统中生产重组纤溶酶原，微纤溶酶原及其片段的方法和组合物。本发明的新发现是浮萍表达系统可用于产生高水平的纤溶酶原和微纤溶酶原。浮萍产生的纤溶酶原和微纤溶酶原可被活化以产生具有蛋白酶活性的多肽。因此，本发明包括用于在表达纤溶酶原，微纤溶酶原及其片段的表达盒和包含编码纤溶酶原，微纤溶酶原的核苷酸序列的核酸的浮萍，浮萍植物中表达纤溶酶原，微纤溶酶原及其片段的方法及其片段，其中这些核苷酸序列被修饰以增强它们在浮萍中的表达。

7. 发明申请

WO2005035767A1 ALPHA INTERFERON VARIANTS 审中-公开
标题翻译：阿尔法干扰素
公开(公告)号：WO2005035767A1
公开(公告)日：2005-04-21
申请号：PCT/US2004/011965
申请日：2004-04-16
申请人： BIOLEX, INC. , DICKEY, Lynn , GASDASKA, John , COX, Kevin
发明人： DICKEY, Lynn , GASDASKA, John , COX, Kevin
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C07K14/56 , C07K2317/55 , C12N15/8257
摘要： The present invention provides biologically active variants of human α-2b- interferon. The variants contain carboxy terminus truncations when compared with the amino acid sequence of full-length human α-2b-interferon. It is the novel finding of the present invention that these truncated variants have the biological activity of full-length human α-2b-interferon. The invention encompasses these biologically active variant α-interferons, as well as polynucleotides encoding these interferons. Expression cassettes comprising these polynucleotides and host cells comprising the expression cassettes are also provided. The invention also provides compositions comprising variant α-interferon polypeptides and a pharmaceutically acceptable carrier.
摘要翻译：本发明提供人α-2b-干扰素的生物活性变体。当与全长人α-2b-干扰素的氨基酸序列相比时，变体含有羧基末端截短。本发明的新发现是这些截短的变体具有全长人α-2b-干扰素的生物学活性。本发明包括这些生物活性变体α-干扰素，以及编码这些干扰素的多核苷酸。还提供了包含这些多核苷酸的表达盒和包含表达盒的宿主细胞。本发明还提供包含变体α-干扰素多肽和药学上可接受的载体的组合物。

8. 发明申请

WO2012174139A3 COMPOSITIONS AND METHODS FOR MAKING AND B IOC ONT AINING AUXOTROPHIC TRANSGENIC PLANTS 审中-公开
公开(公告)号：WO2012174139A3
公开(公告)日：2012-12-20
申请号：PCT/US2012/042286
申请日：2012-06-13
申请人： SYNTHON BIOPHARMACEUTICALS B.V. , COX, Kevin, M. , NGUYEN, Long
发明人： COX, Kevin, M. , NGUYEN, Long
IPC分类号： C12N15/82 , C12N9/00 , C12N9/10 , C12N9/88
摘要： Compositions and methods are described for making and using transgenic plants and plant parts having at least one auxotrophic requirement for an essential compound such as an amino acid, carbohydrate, fatty acid, nucleic acid, vitamin, plant hormone, or precursor thereof. Transgenic plants and plants parts having at least one auxotrophic requirement can be effectively biocontained by withdrawal of the essential compound.

9. 发明申请

WO2012174139A2 COMPOSITIONS AND METHODS FOR MAKING AND B IOC ONT AINING AUXOTROPHIC TRANSGENIC PLANTS 审中-公开
标题翻译：组合物和制备方法和B IOC在澳大利亚奥陶系转基因植物上
公开(公告)号：WO2012174139A2
公开(公告)日：2012-12-20
申请号：PCT/US2012042286
申请日：2012-06-13
申请人： SYNTHON BIOPHARMACEUTICALS BV , COX KEVIN M , NGUYEN LONG
发明人： COX KEVIN M , NGUYEN LONG
IPC分类号： C12N15/82
CPC分类号： C12N15/8238 , C12N9/13 , C12N9/88 , C12N9/93 , C12N15/8218 , C12N15/8251 , C12N15/8257 , C12N15/8265
摘要： Compositions and methods are described for making and using transgenic plants and plant parts having at least one auxotrophic requirement for an essential compound such as an amino acid, carbohydrate, fatty acid, nucleic acid, vitamin, plant hormone, or precursor thereof. Transgenic plants and plants parts having at least one auxotrophic requirement can be effectively biocontained by withdrawal of the essential compound.
摘要翻译：描述了用于制备和使用对于必需化合物如氨基酸，碳水化合物，脂肪酸，核酸，维生素，植物激素或其前体具有至少一种营养缺陷性要求的转基因植物和植物部分的组合物和方法。具有至少一种营养缺陷要求的转基因植物部分可以通过取出必需化合物而被有效地生物保存。

10. 发明申请

WO2010138631A1 COMPOSITIONS AND METHODS FOR PRODUCTION OF AGLYCOSYLATED PLASMINOGEN 审中-公开
标题翻译：用于生产合成的PLASMINOGEN的组合物和方法
公开(公告)号：WO2010138631A1
公开(公告)日：2010-12-02
申请号：PCT/US2010/036253
申请日：2010-05-26
申请人： BIOLEX THERAPEUTICS, INC. , DICKEY, Lynn, F. , GASDASKA, John, R. , COX, Kevin, M.
发明人： DICKEY, Lynn, F. , GASDASKA, John, R. , COX, Kevin, M.
IPC分类号： C12N9/68 , A61K38/16 , C12N15/82 , C12N5/04 , C12N9/14
CPC分类号： A61K38/168 , C12N9/6435 , C12N15/8257 , C12Y304/21007
摘要： Compositions and methods for producing aglycosylated plasminogen (PLG) polypeptides and fragments and variants thereof are provided. Compositions of the invention include isolated nucleic acid molecules encoding aglycosylated PLG polypeptides in which the asparagine (Asn) residue corresponding to residue Asn-289 of the mature human PLG polypeptide has been substituted with an amino acid residue that does not support N -linked glycosylation at that position of the PLG polypeptide, as well as the aglycosylated PLG polypeptides encoded thereby. Expression constructs comprising these PLG-encoding nucleic acid molecules and transgenic plants comprising these expression constructs are also provided. Methods of the invention comprise introducing a PLG-encoding nucleic acid molecule of the invention into a plant of interest and culturing the plant under conditions to produce the aglycosylated PLG polypeptide. The aglycosylated PLG polypeptide allows for significant increases in production and yield of PLG from a plant-based expression system without comprising the ability of the PLG product to be activated to a polypeptide capable of binding fibrin and having serine protease activity, including biologically active plasmin that is also aglycosylated. The activated aglycosylated plasmin is useful to treat diseases or conditions associated with a thrombus.
摘要翻译：提供了用于产生糖基化纤溶酶原（PLG）多肽及其片段和变体的组合物和方法。本发明的组合物包括分离的编码糖基化PLG多肽的核酸分子，其中对应于成熟人PLG多肽的残基Asn-289的天冬酰胺（Asn）残基已被不支持N-连接糖基化的氨基酸残基取代 PLG多肽的位置，以及由此编码的糖基化的PLG多肽。还提供了包含编码这些编码PLG的核酸分子和包含这些表达构建体的转基因植物的表达构建体。本发明的方法包括将本发明的编码PLG的核酸分子引入感兴趣的植物中，并在产生糖苷基化的PLG多肽的条件下培养该植物。糖基化的PLG多肽允许来自基于植物的表达系统的PLG的产量和产量的显着增加，而不包括PLG产物被活化到能够结合纤维蛋白并具有丝氨酸蛋白酶活性的多肽的能力，包括生物活性纤溶酶，也糖基化。活化的糖基化纤溶酶可用于治疗与血栓相关的疾病或病症。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式