专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2007084922A8 COMPOSITIONS AND METHODS FOR HUMANIZATION AND OPTIMIZATION OF N-GLYCANS IN PLANTS 审中-公开
标题翻译： N-GLYCANS在植物中的人类化和优化的组合物和方法
公开(公告)号：WO2007084922A8
公开(公告)日：2009-07-23
申请号：PCT/US2007060642
申请日：2007-01-17
申请人： BIOLEX THERAPEUTICS INC , DICKEY LYNN F , COX KEVIN M , PEELE CHARLES G
发明人： DICKEY LYNN F , COX KEVIN M , PEELE CHARLES G
IPC分类号： C12N15/82 , A01H5/00 , C07K14/47 , C07K16/00 , C12P21/00
CPC分类号： C07K14/47 , C07K16/00 , C07K2317/13 , C07K2317/41 , C07K2317/71 , C07K2317/72 , C07K2317/732 , C07K2317/734 , C12N15/8257 , C12N15/8258
摘要： Methods for altering the N-glycosylation pattern of proteins in higher plants are provided. The methods comprise introducing into the plant a recombinant construct that provides for the inhibition of expression of al,3-fucosyltransferase (FucT) and ß 1,2-xylosyltransferase (XyIT) in a plant. Use of these constructs to inhibit or suppress expression of both of these enzymes, and isoforms thereof, advantageously provides for the production of endogenous and heterologous proteins having a 'humanized' N-glycosylation pattern without impacting plant growth and development. Stably transformed higher plants having this protein N-glycosylation pattern are provided. Glycoprotein compositions, including monoclonal antibody compositions, having substantially homogeneous glycosylation profiles, and which are substantially homogeneous for the GO glycoform, are also provided.
摘要翻译：提供了改变高等植物中蛋白质N-糖基化模式的方法。所述方法包括向植物中引入一种重组构建体，其在植物中提供抑制al-3-岩藻糖基转移酶（FucT）和β-1,2-木糖基转移酶（XyIT）的表达。使用这些构建体来抑制或抑制这两种酶及其同种型的表达，有利地提供具有“人源化”N-糖基化模式的内源和异源蛋白质的生产而不影响植物的生长和发育。提供了具有这种蛋白质N-糖基化模式的稳定转化的高等植物。还提供了糖蛋白组合物，包括具有基本上均匀的糖基化特征的单克隆抗体组合物，并且对于GO糖型基本均匀。

2. 发明申请

WO2007084926A3 COMPOSITIONS AND METHODS FOR HUMANIZATION AND OPTIMIZATION OF N-GLYCANS IN PLANTS 审中-公开
标题翻译：植物中N-糖苷的人工化和优化的组合物和方法
公开(公告)号：WO2007084926A3
公开(公告)日：2008-04-24
申请号：PCT/US2007060646
申请日：2007-01-17
申请人： BIOLEX THERAPEUTICS INC , COMMW SCIENT IND RES ORG , DICKEY LYNN F , COX KEVIN M , PEELE CHARLES G , WANG MING-BO
发明人： DICKEY LYNN F , COX KEVIN M , PEELE CHARLES G , WANG MING-BO
IPC分类号： C07K14/47 , A01H5/00 , C07K16/00 , C07K16/28 , C07K16/32 , C12N15/82 , C12P21/00
CPC分类号： C07K14/47 , C07K16/00 , C07K2317/13 , C07K2317/41 , C07K2317/71 , C07K2317/72 , C07K2317/732 , C07K2317/734 , C12N15/8257 , C12N15/8258
摘要： Methods for altering the N-glycosylation pattern of proteins in higher plants are provided. The methods comprise introducing into the plant a recombinant construct that provides for the inhibition of expression of al,3-fucosyltransferase (FucT) and ß 1 ,2-xylosyltransferase (XyIT) in a plant. Use of these constructs to inhibit or suppress expression of both of these enzymes, and isoforms thereof, advantageously provides for the production of endogenous and heterologous proteins having a "humanized" N-glycosylation pattern without impacting plant growth and development. Stably transformed higher plants having this protein N-glycosylation pattern are provided. Glycoprotein compositions, including monoclonal antibody compositions, having substantially homogeneous glycosylation profiles, and which are substantially homogeneous for the GO glycoform, are also provided.
摘要翻译：提供了改变高等植物中蛋白N-糖基化模式的方法。所述方法包括向植物中导入提供抑制植物中α1,3-岩藻糖基转移酶（FucT）和β1,2-木糖基转移酶（XylT）表达的重组构建体。使用这些构建体来抑制或抑制这些酶及其同种型的表达，有利地提供了具有“人源化”N-糖基化模式的内源和异源蛋白质的生产，而不影响植物生长和发育。提供了具有该蛋白N-糖基化模式的稳定转化的高等植物。还提供了糖蛋白组合物，包括单克隆抗体组合物，其具有基本上均匀的糖基化分布，并且其对于GO糖型基本上是均质的。

3. 发明申请

WO2007124186A3 EXPRESSION CONTROL ELEMENTS FROM THE LEMNACEAE FAMILY 审中-公开
标题翻译：来自LEMNACEAE FAMILY的表达控制元素
公开(公告)号：WO2007124186A3
公开(公告)日：2008-04-24
申请号：PCT/US2007060614
申请日：2007-01-17
申请人： BIOLEX THERAPEUTICS INC , DICKEY LYNN F , COX KEVIN M , PEELE CHARLES G
发明人： DICKEY LYNN F , COX KEVIN M , PEELE CHARLES G
IPC分类号： C07K14/415 , A01H5/00 , C12N15/82
CPC分类号： C12N15/8216 , C07K14/415 , C12N9/2442 , C12N9/88 , C12N15/8221 , C12N15/8222 , C12N15/8223 , C12Y302/01014 , C12Y401/01039
摘要： Compositions and methods for regulating expression of nucleotide sequences of interest in a plant are provided. Compositions include novel nucleic acid molecules, and variants and fragments thereof, for expression control elements isolated from the Lemnaceae ubiquitin, r-histone and chinatase genes. A method for expressing a nucleotide sequence of interest in a plant using the expression control elements disclosed herein is further provided. Also provided is a novel Lemnaceae signal peptide-encoding sequence and the signal peptide encoded thereby. Where an expression construct of the invention is designed to express a polypeptide of interest, this novel signal peptide-encoding sequence can be included within the expression construct of the invention to provide for extracellular secretion of the encoded polypeptide of interest.
摘要翻译：提供了用于调节植物中感兴趣的核苷酸序列的表达的组合物和方法。组合物包括新的核酸分子及其变体和片段，用于从莲子科泛素，r-组蛋白和chinatase基因分离的表达控制元件。还提供了使用本文公开的表达控制元件在植物中表达目标核苷酸序列的方法。还提供了一种新颖的莲子科信号肽编码序列和由其编码的信号肽。当本发明的表达构建体设计用于表达感兴趣的多肽时，该新型信号肽编码序列可以包括在本发明的表达构建体内，以提供所编码的目标多肽的细胞外分泌。

4. 发明申请

WO2005078109A3 EXPRESSION OF PLASMINOGEN AND MICROPLASMINOGEN IN DUCKWEED 审中-公开
标题翻译：杜氏鲍曼不动杆菌的表达及其微丝体表达
公开(公告)号：WO2005078109A3
公开(公告)日：2005-11-10
申请号：PCT/US2005004245
申请日：2005-02-11
申请人： BIOLEX INC , SPENCER DAVID , DICKEY LYNN F , GASDASKA JOHN R , WANG XIAOWEI , COX KEVIN M , PEELE CHARLES G
发明人： SPENCER DAVID , DICKEY LYNN F , GASDASKA JOHN R , WANG XIAOWEI , COX KEVIN M , PEELE CHARLES G
IPC分类号： A01H1/00 , C12N9/68 , C12N15/82
CPC分类号： C12N15/8257 , C12N9/6435 , C12Y304/21007
摘要： The present invention provides methods and compositions for the production of recombinant plasminogen, microplasminogen, and fragments thereof in a duckweed expression system. It is the novel finding of the present invention that a duckweed expression system may be used to produce high levels of plasminogen and microplasminogen. The duckweed-produced plasminogen and microplasminogen can be activated to produce a polypeptide having protease activity. Thus, the invention encompasses methods for the expression of plasminogen, microplasminogen, and fragments thereof in duckweed, duckweed plants that are transformed with expression cassettes for the expression of plasminogen, microplasminogen, and fragments thereof, and nucleic acids comprising nucleotide sequences encoding plasminogen, microplasminogen, and fragments thereof, where these nucleotide sequences are modified to enhance their expression in duckweed.
摘要翻译：本发明提供了用于在浮萍表达系统中生产重组纤溶酶原，微纤溶酶原及其片段的方法和组合物。本发明的新发现是浮萍表达系统可用于产生高水平的纤溶酶原和微纤溶酶原。浮萍产生的纤溶酶原和微纤溶酶原可被活化以产生具有蛋白酶活性的多肽。因此，本发明包括用于在表达纤溶酶原，微纤溶酶原及其片段的表达盒和包含编码纤溶酶原，微纤溶酶原的核苷酸序列的核酸的浮萍，浮萍植物中表达纤溶酶原，微纤溶酶原及其片段的方法及其片段，其中这些核苷酸序列被修饰以增强它们在浮萍中的表达。

5. 发明申请

WO2007084922A2 COMPOSITIONS AND METHODS FOR HUMANIZATION AND OPTIMIZATION OF N-GLYCANS IN PLANTS 审中-公开
标题翻译： N-GLYCANS在植物中的人类化和优化的组合物和方法
公开(公告)号：WO2007084922A2
公开(公告)日：2007-07-26
申请号：PCT/US2007060642
申请日：2007-01-17
申请人： BIOLEX INC , DICKEY LYNN F , COX KEVIN M , PEELE CHARLES G
发明人： DICKEY LYNN F , COX KEVIN M , PEELE CHARLES G
CPC分类号： C07K14/47 , C07K16/00 , C07K2317/13 , C07K2317/41 , C07K2317/71 , C07K2317/72 , C07K2317/732 , C07K2317/734 , C12N15/8257 , C12N15/8258
摘要： Methods for altering the N-glycosylation pattern of proteins in higher plants are provided. The methods comprise introducing into the plant a recombinant construct that provides for the inhibition of expression of al,3-fucosyltransferase (FucT) and ß 1 ,2-xylosyltransferase (XyIT) in a plant. Use of these constructs to inhibit or suppress expression of both of these enzymes, and isoforms thereof, advantageously provides for the production of endogenous and heterologous proteins having a "humanized" N-glycosylation pattern without impacting plant growth and development. Stably transformed higher plants having this protein N-glycosylation pattern are provided. Glycoprotein compositions, including monoclonal antibody compositions, having substantially homogeneous glycosylation profiles, and which are substantially homogeneous for the GO glycoform, are also provided.
摘要翻译：提供了改变高等植物中蛋白质N-糖基化模式的方法。所述方法包括向植物中引入一种重组构建体，其在植物中提供抑制al-3-岩藻糖基转移酶（FucT）和β1-木糖基转移酶（XyIT）的表达。这些构建体用于抑制或抑制这两种酶及其同种型的表达有利地提供具有“人源化”N-糖基化模式的内源和异源蛋白质的生产，而不影响植物的生长和发育。提供了具有这种蛋白质N-糖基化模式的稳定转化的高等植物。还提供了糖蛋白组合物，包括具有基本上均匀的糖基化特征的单克隆抗体组合物，并且对于GO糖型基本均匀。

6. 发明申请

WO2007084672A2 MONOCLONAL ANTIBODIES AGAINST CD30 LACKING IN FUCOSYL AND XYLOSYL RESIDUES 审中-公开
标题翻译：单克隆抗体对抗CD30在纤维素和木糖残留物中的缺乏
公开(公告)号：WO2007084672A2
公开(公告)日：2007-07-26
申请号：PCT/US2007001451
申请日：2007-01-17
申请人： MEDAREX INC , BLACK AMELIA NANCY , PASSMORE DAVID B , SRINIVASAN MOHAN , DICKEY LYNN F , COX KEVIN M , PEELE CHARLES G , WANG MING-BO
发明人： BLACK AMELIA NANCY , PASSMORE DAVID B , SRINIVASAN MOHAN , DICKEY LYNN F , COX KEVIN M , PEELE CHARLES G , WANG MING-BO
IPC分类号： C07K16/00 , A61K39/395 , A61P35/00 , A61P37/00 , C07K16/28
CPC分类号： C07K16/2878 , A61K2039/505 , C07K2317/13 , C07K2317/21 , C07K2317/41 , C07K2317/72 , C07K2317/732
摘要： The invention pertains to anti-CD30 antibodies that lack fucosyl and xylosyl residues. The antibodies of the invention exhibit increased antibody-dependent cellular cytotoxicity (ADCC) activity, including the ability to Iyse CD30-expressing cell lines that are not lysed by the fucosylated and xylosylated form of the antibodies. The invention also provides host cells that express the anti-CD30 antibodies that lack fucosyl and xylosyl residues, wherein the host cells are deficient for a fucosyltransferase and a xylosyltransferase. Methods of using the antibodies to inhibit the growth of CD30 + cells, such as tumor cells, are also provided.
摘要翻译：本发明涉及缺乏岩藻糖基和木糖基残基的抗CD30抗体。本发明的抗体表现出增加的抗体依赖性细胞毒性（ADCC）活性，包括能够表达抗原的岩藻糖基化和木糖基化形式不裂解的表达CD30的细胞系。本发明还提供了表达缺乏岩藻糖基和木糖基残基的抗CD30抗体的宿主细胞，其中宿主细胞对于岩藻糖基转移酶和木糖基转移酶是不足的。还提供了使用抗体抑制CD30 + / +细胞如肿瘤细胞生长的方法。

7. 发明申请

WO2010096545A3 ASEPTIC BIOREACTOR SYSTEM FOR PROCESSING BIOLOGICAL MATERIALS 审中-公开
标题翻译：用于处理生物材料的无菌生物反应器系统
公开(公告)号：WO2010096545A3
公开(公告)日：2010-11-25
申请号：PCT/US2010024570
申请日：2010-02-18
申请人： BIOLEX THERAPEUTICS INC , EVERETT KEITH , DICKEY LYNN F
发明人： EVERETT KEITH , DICKEY LYNN F
IPC分类号： C12M1/00 , C12M1/24
CPC分类号： C12M33/14 , C12M21/02 , C12M23/14 , C12M23/44 , C12M23/48
摘要： A bioreactor system according to one embodiment of the present invention is provided for producing and processing a biological material in an aseptic environment. For example, the system includes at least one culture tube configured to hold a liquid media containing a biological material and at least one flexible bag for promoting growth of the biological material therein. The liquid media and biological material are configured to be transferred from the culture tube to the flexible bag aseptically in an unclassified area. The system also includes at least one flexible harvest bag configured to be in flow communication with the flexible bag, wherein the flexible harvest bag is configured to separate biological material grown in the flexible bag from the liquid media aseptically in an unclassified area.
摘要翻译：提供根据本发明的一个实施例的生物反应器系统用于在无菌环境中生产和处理生物材料。例如，该系统包括至少一个配置为容纳含有生物材料的液体培养基的培养管和至少一个用于促进生物材料在其中生长的柔性袋。液体培养基和生物材料被配置为在未分类的区域中无菌地从培养管转移到柔性袋。该系统还包括构造成与柔性袋流动连通的至少一个柔性收获袋，其中柔性收获袋构造成在未分类区域中无菌地将生长在柔性袋中的生物材料与液体介质分离。

8. 发明申请

WO2010096545A8 ASEPTIC BIOREACTOR SYSTEM FOR PROCESSING BIOLOGICAL MATERIALS 审中-公开
标题翻译：用于处理生物材料的自由生物反应器系统
公开(公告)号：WO2010096545A8
公开(公告)日：2010-10-07
申请号：PCT/US2010024570
申请日：2010-02-18
申请人： BIOLEX THEPAPEUTICS INC , EVERETT KEITH , DICKEY LYNN F
发明人： EVERETT KEITH , DICKEY LYNN F
IPC分类号： C12M1/00
CPC分类号： C12M33/14 , C12M21/02 , C12M23/14 , C12M23/44 , C12M23/48
摘要： A bioreactor system according to one embodiment of the present invention is provided for producing and processing a biological material in an aseptic environment. For example, the system includes at least one culture tube configured to hold a liquid media containing a biological material and at least one flexible bag for promoting growth of the biological material therein. The liquid media and biological material are configured to be transferred from the culture tube to the flexible bag aseptically in an unclassified area. The system also includes at least one flexible harvest bag configured to be in flow communication with the flexible bag, wherein the flexible harvest bag is configured to separate biological material grown in the flexible bag from the liquid media aseptically in an unclassified area.
摘要翻译：提供根据本发明的一个实施方案的生物反应器系统，用于在无菌环境中生产和加工生物材料。例如，该系统包括构造成容纳含有生物材料的液体介质和至少一个用于促进生物材料生长的柔性袋的至少一个培养管。液体介质和生物材料构造成在未分类区域中无菌地从培养管转移到柔性袋。该系统还包括构造成与柔性袋流动连通的至少一个柔性收获袋，其中，所述柔性收获袋被配置为在未分类区域中将在柔性袋中生长的生物材料与液体介质无菌地分离。

9. 发明申请

WO2010063033A3 RECOMBINANT AVIAN INFLUENZA VACCINE AND USES THEREOF 审中-公开
标题翻译：重组AVIAN流感疫苗及其用途
公开(公告)号：WO2010063033A3
公开(公告)日：2010-10-28
申请号：PCT/US2009066146
申请日：2009-11-30
申请人： MERIAL LTD , BIOLEX THERAPEUTICS , GUO XUAN , BUBLOT MICHEL , PRITCHARD JOYCE A , DICKEY LYNN F
发明人： GUO XUAN , BUBLOT MICHEL , PRITCHARD JOYCE A , DICKEY LYNN F
IPC分类号： A61K39/00
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/517 , A61K2039/545 , A61K2039/55566 , C12N15/8257 , C12N15/8258 , C12N2760/16134
摘要： The present invention encompasses influenza vaccines, in particular avian influenza vaccines. The vaccine may be a subunit vaccine based on the hemagglutinin of influenza. The hemagglutinin may be expressed in plants including duckweed. The invention also encompasses recombinant vectors encoding and expressing influenza antigens, epitopes or immunogens which can be used to protect animals against influenza. It encompasses also a vaccination regimen compatible with the DIVA strategy, including a prime-boost scheme using vector and subunit vaccines.
摘要翻译：本发明包括流感疫苗，特别是禽流感疫苗。疫苗可以是基于流感血凝素的亚单位疫苗。血凝素可以在植物中表达，包括浮萍。本发明还包括编码和表达可用于保护动物免受流感的流感抗原，表位或免疫原的重组载体。它还包括与DIVA策略兼容的疫苗接种方案，包括使用载体和亚单位疫苗的初始 - 升高方案。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式