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    • 1. 发明申请
    • EPIGENETICALLY STABLE CLONED PLANTS
    • 特殊稳定的克隆植物
    • WO2017153766A1
    • 2017-09-14
    • PCT/GB2017/050640
    • 2017-03-10
    • UNIVERSITY OF WARWICK
    • GUTIERREZ-MARCOS, JoseWIBOWO, Anjar
    • C12N15/82
    • C12N15/8222C12N15/8238
    • A method of cloning plants involves a reprogramming of somatic plant cells of a selected parent plant into undifferentiated initials by expressing RKD4, BBM, LEC2 or FUS3 transcription factors using a two-component inducible expression system. New plants are then recovered by growing the transformed cells in the absence of the inducer. Regenerated plants are fertile and retain epigenetic characteristics of the somatic cells prior to the reprogramming. No changes in gene sequence take place. Regenerated plants are selected so they have at least one epigenetic characteristic of the parent plant. This approach allows a plant, for example Elaeis guineensis or Phalaenopsis sp . with desirable epigenetic character to be cloned.
    • 克隆植物的方法涉及通过使用双组分诱导型表达系统表达RKD4,BBM,LEC2或FUS3转录因子,将所选亲本植物的体细胞植物细胞重编程为未分化的起始密码子。 然后通过在没有诱导物的情况下培养转化的细胞来回收新植物。 再生植物是可育的并且在重编程之前保留体细胞的表观遗传特征。 基因序列没有发生变化。 选择再生植物以使它们具有至少一个亲本植物的表观遗传特征。 这种方法允许植物,例如Elaeis guineensis或蝴蝶兰sp。 并具有理想的表观遗传特性。
    • 6. 发明申请
    • RIPENING PROMOTER
    • WO2011016841A2
    • 2011-02-10
    • PCT/US2010002110
    • 2010-07-28
    • UNIV CORNELLHRAZDINA GEZAKARAASLAN MEHMET
    • HRAZDINA GEZAKARAASLAN MEHMET
    • A01H5/00C12N15/29C12N15/82
    • C12N15/8235C07K14/415C12N15/8233C12N15/8238
    • The presence of expansins was investigated in various developmental and ripening stages of cherry fruits by SDS PAGE and immunoblotting. An expansin gene and three fragments (242, 607, and 929 bp) of its promoter region were cloned. The genomic clone of the expansin gene contained three introns, two exons spanning a 1.6 kb and a 1.0 kb upstream region. Semi quantitative PCR analysis showed that this gene was ripening specific. Chimeric promoter - GUS constructs were made and truncated forms of the expansin promoter were introduced into tomatoes by agroinjection and fruits were analyzed for GUS expression by histochemical GUS staining and enzyme activity assays. The 0.60 kb expansin promoter efficiently induced GUS expression in transgenic tomatoes, whereas constructs with the 0.25 kb promoter did not display significant GUS staining. The highest GUS activity was detected in tomatoes containing the 1.0 kb promoter construct. Both large base pair promoter constructs drove the expression of the GUS gene at an equal or higher rate than the tomato E8 promoter.
    • 通过SDS PAGE和免疫印迹在樱桃果实的各种发育和成熟阶段研究了膨胀物的存在。 克隆了其启动子区的扩展蛋白基因和三个片段(242,667和929bp)。 扩展蛋白基因的基因组克隆包含三个内含子,两个外显子跨越1.6kb和1.0kb上游区。 半定量PCR分析表明该基因成熟。 制备嵌合启动子 - GUS构建体,通过农杆菌引入扩增蛋白启动子的截短形式进入番茄,并通过组织化学GUS染色和酶活性测定分析果实的GUS表达。 0.60kb的扩增蛋白启动子有效地诱导转基因番茄中的GUS表达,而具有0.25kb启动子的构建体没有显示出显着的GUS染色。 在含有1.0kb启动子构建体的番茄中检测到最高的GUS活性。 两个大的碱基对启动子构建体以与番茄E8启动子相同或更高的速率驱动GUS基因的表达。
    • 7. 发明申请
    • CONTROL OF KEY ETHYLENE HORMONE SIGNALING PATHWAY PROTEINS IN PLANTS
    • 关键乙烯激素信号通路蛋白在植物中的控制
    • WO2010101884A1
    • 2010-09-10
    • PCT/US2010/025872
    • 2010-03-02
    • ROHM AND HAAS COMPANYROSICHAN, Jeffrey, L.
    • ROSICHAN, Jeffrey, L.
    • C12N15/00
    • C12N15/8291C12N15/8216C12N15/8217C12N15/8238C12N15/8249C12N15/8261C12N15/8266C12N15/8267Y02A40/146
    • A gene expression system for controllable expression of ethylene response in a plant cell includes an activation cassette comprising a DNA-binding domain that recognizes a response element; an ecdysone receptor ligand binding domain; and an activation domain; and a target cassette comprising an inducible promoter, which comprises, in operative association, the response element and a minimal promoter responsive to the activation domain. The inducible promoter controls the expression of a nucleic acid sequence that encodes a selected regulatory protein that modifies sensitivity to ethylene of certain signal proteins in the plant. Interaction among the components of the activation cassette and target cassette, when in a plant cell, in the presence of an inducing composition, increases expression of the selected regulatory protein, and in turn decreases expression and accumulation of the signal protein in the plant, thereby and decreasing ethylene sensitivity in the plant cell. This increase in the expression of the regulatory protein, particularly in the presence of ethylene, is controlled by the timing, the concentration and the duration of the application of the inducing composition. Transgenic plant cells, tissues, organs and entire plants are provided, which in the presence of the inducing composition control ethylene sensitivity. Ethylene sensitivity and/or ethylene production in such transgenic plants and tissues may be controlled for purposes of manipulating ripening, flower senescence and other ethylene sensitive functions of the plant.
    • 用于在植物细胞中可控表达乙烯应答的基因表达系统包括活化盒,其包含识别响应元件的DNA结合结构域; 蜕皮激素受体配体结合域; 和激活域; 以及包含可诱导启动子的靶盒,其包含有效关联的响应元件和响应于激活结构域的最小启动子。 诱导型启动子控制编码选择的调节蛋白的核酸序列的表达,其修饰植物中某些信号蛋白的乙烯的敏感性。 当在植物细胞中存在诱导组合物时,活化盒和靶盒的组分之间的相互作用增加所选调节蛋白的表达,进而降低信号蛋白在植物中的表达和积累,从而 并降低植物细胞中的乙烯敏感性。 调节蛋白表达的这种增加,特别是在乙烯存在下,受诱导组合物施用的时间,浓度和持续时间的控制。 提供转基因植物细胞,组织,器官和整个植物,其在诱导组合物存在下控制乙烯敏感性。 可以控制这些转基因植物和组织中的乙烯敏感性和/或乙烯产生,用于操作植物的成熟,花衰老和其它乙烯敏感功能。
    • 9. 发明申请
    • PLANT GENES INVOLVED IN NITRATE UPTAKE AND METABOLISM
    • 涉及硝酸盐摄取和代谢的植物基因
    • WO2008073578A8
    • 2009-07-09
    • PCT/US2007082123
    • 2007-10-22
    • UNIV IOWA STATE RES FOUND INCSCHNABLE PATRICK SDASH SUDHANSU
    • SCHNABLE PATRICK SDASH SUDHANSU
    • A01H1/00C07H21/04C07K14/415C12N15/00
    • C12N15/8261C07K14/415C12N15/8222C12N15/8238
    • The present invention relates nucleic acid molecules that are modulated (e.g., upregulated) by nitrogen in corn, to proteins or polypeptides encoded by these nucleic acid molecules, and promoters of these nucleic acid molecules. The present invention relates to a nucleic acid construct having a nucleic acid molecule that is modulated by nitrogen in corn, as well as to expression systems, host cells, plants, and plant seeds having the nucleic acid construct. The present invention also relates to a method of expressing the nucleic acid molecule that is modulated by nitrogen in a plant by growing a transgenic plant or a plant grown from a transgenic seed transformed with the construct. The present invention further relates to an isolated DNA promoter that can be used to direct nitrogen-regulated expression of an isolated nucleic acid in plants.
    • 本发明涉及由玉米中的氮调节(例如上调)到由这些核酸分子编码的蛋白质或多肽的核酸分子,以及这些核酸分子的启动子。 本发明涉及具有由玉米中的氮调节的核酸分子以及具有核酸构建体的表达系统,宿主细胞,植物和植物种子的核酸构建体。 本发明还涉及通过生长转基因植物或从用该构建体转化的转基因种子生长的植物来表达在植物中由氮调节的核酸分子的方法。 本发明还涉及可用于将分离的核酸的氮调节表达引导到植物中的分离的DNA启动子。