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1. 发明申请

WO2017153766A1 EPIGENETICALLY STABLE CLONED PLANTS 审中-公开
标题翻译：特殊稳定的克隆植物
公开(公告)号：WO2017153766A1
公开(公告)日：2017-09-14
申请号：PCT/GB2017/050640
申请日：2017-03-10
申请人： UNIVERSITY OF WARWICK
发明人： GUTIERREZ-MARCOS, Jose , WIBOWO, Anjar
IPC分类号： C12N15/82
CPC分类号： C12N15/8222 , C12N15/8238
摘要： A method of cloning plants involves a reprogramming of somatic plant cells of a selected parent plant into undifferentiated initials by expressing RKD4, BBM, LEC2 or FUS3 transcription factors using a two-component inducible expression system. New plants are then recovered by growing the transformed cells in the absence of the inducer. Regenerated plants are fertile and retain epigenetic characteristics of the somatic cells prior to the reprogramming. No changes in gene sequence take place. Regenerated plants are selected so they have at least one epigenetic characteristic of the parent plant. This approach allows a plant, for example Elaeis guineensis or Phalaenopsis sp . with desirable epigenetic character to be cloned.
摘要翻译：克隆植物的方法涉及通过使用双组分诱导型表达系统表达RKD4，BBM，LEC2或FUS3转录因子，将所选亲本植物的体细胞植物细胞重编程为未分化的起始密码子。然后通过在没有诱导物的情况下培养转化的细胞来回收新植物。再生植物是可育的并且在重编程之前保留体细胞的表观遗传特征。基因序列没有发生变化。选择再生植物以使它们具有至少一个亲本植物的表观遗传特征。这种方法允许植物，例如Elaeis guineensis或蝴蝶兰sp。并具有理想的表观遗传特性。

2. 发明申请

WO2015177659A1 SYNTHETIC PROMOTER CONSTRUCT FOR TRANSGENE EXPRESSION 审中-公开
标题翻译：用于转基因表达的合成促进剂构建体
公开(公告)号：WO2015177659A1
公开(公告)日：2015-11-26
申请号：PCT/IB2015/052503
申请日：2015-04-07
申请人： AZARGEN BIOTECHNOLOGIES (PTY) LTD
发明人： ZWIEGELAAR, Jacobus Petrus , VENTER, Mauritz
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8216 , C07K14/785 , C07K2319/60 , C12N15/8222 , C12N15/8238 , C12N15/8257
摘要： The invention is directed to synthetic promoter constructs for enhanced transgene expression in plants and to expression cassettes comprising the synthetic promoter constructs. The expression cassettes may include various elements for improved expression, stability of the expressed protein or efficient purification of the expressed protein, including signal sequences, protease cleavage sites for release of the target protein, trafficking peptides for trafficking of the expressed protein to various plant compartments, and/or various tags. The invention further relates to methods of expression of transgenic proteins in plants with the use of the synthetic promoter constructs and expression cassettes.
摘要翻译：本发明涉及用于增强植物中转基因表达的合成启动子构建体和包含合成启动子构建体的表达盒。表达盒可以包括用于改善表达的各种元件，表达的蛋白质的稳定性或表达的蛋白质的有效纯化，包括信号序列，用于释放靶蛋白的蛋白酶切割位点，用于将表达的蛋白质运送到各种植物区室的肽，和/或各种标签。本发明还涉及使用合成启动子构建体和表达盒在植物中表达转基因蛋白质的方法。

3. 发明申请

WO2014097226A2 REGULATION OF GENE EXPRESSION 审中-公开
标题翻译：基因表达调控
公开(公告)号：WO2014097226A2
公开(公告)日：2014-06-26
申请号：PCT/IB2013/061166
申请日：2013-12-20
申请人： THE NEW ZEALAND INSTITUTE FOR PLANT AND FOOD RESEARCH LIMITED
发明人： LAING, William, Alister , HELLENS, Roger, Paul , MACKNIGHT, Richard, Colin , BULLEY, Sean, Michael, Winsley
CPC分类号： A01H1/06 , C12N9/1241 , C12N15/8238 , C12N15/8243 , C12Q1/6895
摘要： The invention provides isolated polynucleotides comprising sequences encoding a u ORF peptides and variants and fragments thereof. The invention also provides constructs and vectors containing the polynucleotides. The invention further provides cells, plant cells and plants transformed with the polynucleotides and constructs. The invention also provides methods of using the polynucleotides to control expression of operably linked polynucleotides. The invention also provides methods of manipulating GDP-L-Galactose phosphorylase (GGP) expression and ascorbate production in plants utilising the polynucleotides of the invention.
摘要翻译：本发明提供了包含编码u ORF肽及其变体和片段的序列的分离的多核苷酸。本发明还提供含有多核苷酸的构建体和载体。本发明还提供了用多核苷酸和构建体转化的细胞，植物细胞和植物。本发明还提供了使用多核苷酸来控制可操作连接的多核苷酸的表达的方法。本发明还提供了利用本发明的多核苷酸操纵植物中GDP-L-半乳糖磷酸化酶（GGP）表达和抗坏血酸产生的方法。

4. 发明申请

WO2012099664A1 METHODS AND COMPOSITIONS FOR MODIFIED ETHANOL INDUCIBLE PROMOTER SYSTEMS 审中-公开
标题翻译：改性乙醇可溶性促进剂体系的方法和组合
公开(公告)号：WO2012099664A1
公开(公告)日：2012-07-26
申请号：PCT/US2011/065184
申请日：2011-12-15
申请人： SYNGENTA PARTICIPATIONS AG , QUEENSLAND UNIVERSITY OF TECHNOLOGY , SAINZ, Manuel , KINKEMA, Mark David
发明人： SAINZ, Manuel , KINKEMA, Mark David
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12N15/8238 , C12N15/113 , C12N15/8241
摘要： The present invention provides nucleic acid molecules comprising one or more nucleotide sequences selected from the group consisting of the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and/or SEQ ID NO:10 that can be operably linked to a promoter, thereby making the promoter inducible by a chemical compound that can induce the expression of the alcohol dehydrogenase system of Aspergillus nidulans . Methods for making an inducible promoter and for making the expression of a nucleotide sequence of interest inducible are also provided. Further provided are plants, plant parts, and plant cells, comprising the compositions of the present invention.
摘要翻译：本发明提供了包含一个或多个选自下组的核酸序列的核酸分子：SEQ ID NO：1，SEQ ID NO：3，SEQ ID NO：4，SEQ ID NO：5，SEQ ID NO：：6，SEQ ID NO：7，SEQ ID NO：8和/或SEQ ID NO：10，其可以可操作地连接到启动子，从而使启动子可诱导可诱导醇脱氢酶表达的化学化合物构巢曲霉系统。还提供了制备诱导型启动子和用于诱导感兴趣的核苷酸序列的表达的方法。还提供植物，植物部分和植物细胞，其包含本发明的组合物。

5. 发明申请

WO2012071549A2 INDUCIBLE EXPRESSION SYSTEM TRANSCRIPTION MODULATORS COMPRISING A DISTRIBUTED PROTEIN TRANSDUCTION DOMAIN AND METHODS FOR USING THE SAME 审中-公开
标题翻译：包含分布式蛋白质转导域的可诱导表达系统转录调节子及其使用方法
公开(公告)号：WO2012071549A2
公开(公告)日：2012-05-31
申请号：PCT/US2011062115
申请日：2011-11-23
申请人： CLONTECH LAB INC , BISGROVE DWAYNE , SAGAWA HIROAKI
发明人： BISGROVE DWAYNE , SAGAWA HIROAKI
IPC分类号： C12N15/11 , C12N5/10 , C12N15/63 , C12N15/85
CPC分类号： C12N15/635 , C07K14/4703 , C07K14/4705 , C07K2319/10 , C07K2319/80 , C07K2319/81 , C12N15/8217 , C12N15/8238
摘要： Aspects of the invention include inducible expression systems in which a transcription modulator having a distributed protein transduction domain is employed. Aspects of the invention further include methods of using the systems to induce expression of a coding sequence, as well as kits that find use in practicing methods of the invention. The systems, components thereof, methods and kits find use in a variety of different applications.
摘要翻译：本发明的方面包括其中使用具有分布的蛋白质转导结构域的转录调节物的诱导型表达系统。本发明的方面还包括使用该系统诱导编码序列表达的方法，以及在实践本发明方法中使用的试剂盒。系统，组件，方法和套件可用于各种不同的应用。

6. 发明申请

WO2011016841A2 RIPENING PROMOTER 审中-公开
公开(公告)号：WO2011016841A2
公开(公告)日：2011-02-10
申请号：PCT/US2010002110
申请日：2010-07-28
申请人： UNIV CORNELL , HRAZDINA GEZA , KARAASLAN MEHMET
发明人： HRAZDINA GEZA , KARAASLAN MEHMET
IPC分类号： A01H5/00 , C12N15/29 , C12N15/82
CPC分类号： C12N15/8235 , C07K14/415 , C12N15/8233 , C12N15/8238
摘要： The presence of expansins was investigated in various developmental and ripening stages of cherry fruits by SDS PAGE and immunoblotting. An expansin gene and three fragments (242, 607, and 929 bp) of its promoter region were cloned. The genomic clone of the expansin gene contained three introns, two exons spanning a 1.6 kb and a 1.0 kb upstream region. Semi quantitative PCR analysis showed that this gene was ripening specific. Chimeric promoter - GUS constructs were made and truncated forms of the expansin promoter were introduced into tomatoes by agroinjection and fruits were analyzed for GUS expression by histochemical GUS staining and enzyme activity assays. The 0.60 kb expansin promoter efficiently induced GUS expression in transgenic tomatoes, whereas constructs with the 0.25 kb promoter did not display significant GUS staining. The highest GUS activity was detected in tomatoes containing the 1.0 kb promoter construct. Both large base pair promoter constructs drove the expression of the GUS gene at an equal or higher rate than the tomato E8 promoter.
摘要翻译：通过SDS PAGE和免疫印迹在樱桃果实的各种发育和成熟阶段研究了膨胀物的存在。克隆了其启动子区的扩展蛋白基因和三个片段（242,667和929bp）。扩展蛋白基因的基因组克隆包含三个内含子，两个外显子跨越1.6kb和1.0kb上游区。半定量PCR分析表明该基因成熟。制备嵌合启动子 - GUS构建体，通过农杆菌引入扩增蛋白启动子的截短形式进入番茄，并通过组织化学GUS染色和酶活性测定分析果实的GUS表达。 0.60kb的扩增蛋白启动子有效地诱导转基因番茄中的GUS表达，而具有0.25kb启动子的构建体没有显示出显着的GUS染色。在含有1.0kb启动子构建体的番茄中检测到最高的GUS活性。两个大的碱基对启动子构建体以与番茄E8启动子相同或更高的速率驱动GUS基因的表达。

7. 发明申请

WO2010101884A1 CONTROL OF KEY ETHYLENE HORMONE SIGNALING PATHWAY PROTEINS IN PLANTS 审中-公开
标题翻译：关键乙烯激素信号通路蛋白在植物中的控制
公开(公告)号：WO2010101884A1
公开(公告)日：2010-09-10
申请号：PCT/US2010/025872
申请日：2010-03-02
申请人： ROHM AND HAAS COMPANY , ROSICHAN, Jeffrey, L.
发明人： ROSICHAN, Jeffrey, L.
IPC分类号： C12N15/00
CPC分类号： C12N15/8291 , C12N15/8216 , C12N15/8217 , C12N15/8238 , C12N15/8249 , C12N15/8261 , C12N15/8266 , C12N15/8267 , Y02A40/146
摘要： A gene expression system for controllable expression of ethylene response in a plant cell includes an activation cassette comprising a DNA-binding domain that recognizes a response element; an ecdysone receptor ligand binding domain; and an activation domain; and a target cassette comprising an inducible promoter, which comprises, in operative association, the response element and a minimal promoter responsive to the activation domain. The inducible promoter controls the expression of a nucleic acid sequence that encodes a selected regulatory protein that modifies sensitivity to ethylene of certain signal proteins in the plant. Interaction among the components of the activation cassette and target cassette, when in a plant cell, in the presence of an inducing composition, increases expression of the selected regulatory protein, and in turn decreases expression and accumulation of the signal protein in the plant, thereby and decreasing ethylene sensitivity in the plant cell. This increase in the expression of the regulatory protein, particularly in the presence of ethylene, is controlled by the timing, the concentration and the duration of the application of the inducing composition. Transgenic plant cells, tissues, organs and entire plants are provided, which in the presence of the inducing composition control ethylene sensitivity. Ethylene sensitivity and/or ethylene production in such transgenic plants and tissues may be controlled for purposes of manipulating ripening, flower senescence and other ethylene sensitive functions of the plant.
摘要翻译：用于在植物细胞中可控表达乙烯应答的基因表达系统包括活化盒，其包含识别响应元件的DNA结合结构域; 蜕皮激素受体配体结合域; 和激活域; 以及包含可诱导启动子的靶盒，其包含有效关联的响应元件和响应于激活结构域的最小启动子。诱导型启动子控制编码选择的调节蛋白的核酸序列的表达，其修饰植物中某些信号蛋白的乙烯的敏感性。当在植物细胞中存在诱导组合物时，活化盒和靶盒的组分之间的相互作用增加所选调节蛋白的表达，进而降低信号蛋白在植物中的表达和积累，从而并降低植物细胞中的乙烯敏感性。调节蛋白表达的这种增加，特别是在乙烯存在下，受诱导组合物施用的时间，浓度和持续时间的控制。提供转基因植物细胞，组织，器官和整个植物，其在诱导组合物存在下控制乙烯敏感性。可以控制这些转基因植物和组织中的乙烯敏感性和/或乙烯产生，用于操作植物的成熟，花衰老和其它乙烯敏感功能。

8. 发明申请

WO2010079430A1 MODULAR DNA-BINDING DOMAINS AND METHODS OF USE 审中-公开
标题翻译：模块化DNA结合域及其使用方法
公开(公告)号：WO2010079430A1
公开(公告)日：2010-07-15
申请号：PCT/IB2010/000154
申请日：2010-01-12
申请人： BONAS, Ulla , BOCH, Jens , SCHORNACK, Sebastian , LAHAYE, Thomas
发明人： BONAS, Ulla , BOCH, Jens , SCHORNACK, Sebastian , LAHAYE, Thomas
IPC分类号： C07K14/415 , C12N15/82 , G01N33/50
CPC分类号： C12N15/8217 , C07K14/195 , C07K2319/80 , C12N9/22 , C12N15/8237 , C12N15/8238 , C12N15/8239 , C12N15/825 , C12N15/8279 , C12N15/8281
摘要： The present invention refers to methods for selectively recognizing a base pair in a DNA sequence by a polypeptide, to modified polypeptides which specifically recognize one or more base pairs in a DNA sequence and, to DNA which is modified so that it can be specifically recognized by a polypeptide and to uses of the polypeptide and DNA in specific DNA targeting as well as to methods of modulating expression of target genes in a cell.
摘要翻译：本发明涉及通过多肽选择性地识别DNA序列中的碱基对的方法，特异性识别DNA序列中的一个或多个碱基对的修饰的多肽，以及被修饰以使其可以被多肽和多肽和DNA在特异性DNA靶向中的用途以及调节细胞中靶基因表达的方法。

9. 发明申请

WO2008073578A8 PLANT GENES INVOLVED IN NITRATE UPTAKE AND METABOLISM 审中-公开
标题翻译：涉及硝酸盐摄取和代谢的植物基因
公开(公告)号：WO2008073578A8
公开(公告)日：2009-07-09
申请号：PCT/US2007082123
申请日：2007-10-22
申请人： UNIV IOWA STATE RES FOUND INC , SCHNABLE PATRICK S , DASH SUDHANSU
发明人： SCHNABLE PATRICK S , DASH SUDHANSU
IPC分类号： A01H1/00 , C07H21/04 , C07K14/415 , C12N15/00
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8222 , C12N15/8238
摘要： The present invention relates nucleic acid molecules that are modulated (e.g., upregulated) by nitrogen in corn, to proteins or polypeptides encoded by these nucleic acid molecules, and promoters of these nucleic acid molecules. The present invention relates to a nucleic acid construct having a nucleic acid molecule that is modulated by nitrogen in corn, as well as to expression systems, host cells, plants, and plant seeds having the nucleic acid construct. The present invention also relates to a method of expressing the nucleic acid molecule that is modulated by nitrogen in a plant by growing a transgenic plant or a plant grown from a transgenic seed transformed with the construct. The present invention further relates to an isolated DNA promoter that can be used to direct nitrogen-regulated expression of an isolated nucleic acid in plants.
摘要翻译：本发明涉及由玉米中的氮调节（例如上调）到由这些核酸分子编码的蛋白质或多肽的核酸分子，以及这些核酸分子的启动子。本发明涉及具有由玉米中的氮调节的核酸分子以及具有核酸构建体的表达系统，宿主细胞，植物和植物种子的核酸构建体。本发明还涉及通过生长转基因植物或从用该构建体转化的转基因种子生长的植物来表达在植物中由氮调节的核酸分子的方法。本发明还涉及可用于将分离的核酸的氮调节表达引导到植物中的分离的DNA启动子。

10. 发明申请

WO2008133643A3 PLANT MICRORNAS AND METHODS OF USE THEREOF 审中-公开
标题翻译：植物微生物及其使用方法
公开(公告)号：WO2008133643A3
公开(公告)日：2009-05-14
申请号：PCT/US2007021987
申请日：2007-10-12
申请人： MONSANTO TECHNOLOGY LLC , ALLEN EDWARDS , GOLDMAN BARRY S , GUO LIANG , HEISEL SARA E , HUANG SHIHSHIEH , IVASHUTA SERGEY I , KRIEGER ELYSIA K , ROBERTS JAMES K , ZHANG YUANJI I
发明人： ALLEN EDWARDS , GOLDMAN BARRY S , GUO LIANG , HEISEL SARA E , HUANG SHIHSHIEH , IVASHUTA SERGEY I , KRIEGER ELYSIA K , ROBERTS JAMES K , ZHANG YUANJI I
IPC分类号： C12Q1/68 , A01H1/00
CPC分类号： C12N15/113 , C12N15/8216 , C12N15/8218 , C12N15/8238 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12N15/8286 , C12N2310/141 , C12N2320/50 , Y02A40/146 , Y02A40/162
摘要： This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.
摘要翻译：本发明公开了新的微小RNA及其前体，以及重组DNA构建体，包括这样的新型miRNA，miRNA前体，miRNA启动子和对应于miRNA的miRNA识别位点。包括显示营养反应表达的新型miRNA和miRNA前体。还公开了miRNA诱饵序列。还提供了非天然转基因植物细胞，植物和种子，在其基因组中含有本发明的重组DNA构建体和使用本发明的重组DNA构建体控制基因表达的方法。

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