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1. 发明申请

WO2019154520A1 DOWNY MILDEW RESISTANT SUNFLOWER PLANTS 审中-公开
公开(公告)号：WO2019154520A1
公开(公告)日：2019-08-15
申请号：PCT/EP2018/053423
申请日：2018-02-12
申请人： SCIENZA BIOTECHNOLOGIES 5 B.V.
发明人： VAN SCHIE, Christianus Cornelis Nicolaas , ZEILMAKER, Tieme
IPC分类号： A01H5/02 , C12N15/82 , A01H5/10
CPC分类号： C12N15/8282 , A01H5/02 , A01H5/10 , A01H6/14 , C12N15/8251
摘要： The present invention relates to sunflower plants ( Helianthus annuus ) being resistant to the plant disease downy mildew and especially to sunflower plants ( Helianthus annuus ) being resistant to the downy mildew disease causing pathogen Plasmopara halstedii . The present invention further relates to seeds and plant part of the present plants and to methods for identifying downy mildew resistant sunflower plants. Specifically, the present invention relates to Helianthus annuus plants being resistant to downy mildew disease wherein the resistance is provided by one or more amino acid substitutions in the homoserine kinase protein shown in SEQ ID No. 1 or a reduced expression of the gene encoding SEQ ID No.1.

2. 发明申请

WO2016012346A1 STAY GREEN CUCUMBER PLANT 审中-公开
标题翻译：保持绿色的CUCUMBER植物
公开(公告)号：WO2016012346A1
公开(公告)日：2016-01-28
申请号：PCT/EP2015/066310
申请日：2015-07-16
申请人： ENZA ZADEN BEHEER B.V.
发明人： DE BOER, Geert Johannes , GROOT, Mattheus Nicolaas , LOUWERSE, Jeanine Danielle
IPC分类号： A01H1/04 , A01H5/08 , C07K14/415 , C12N15/82
CPC分类号： A01H5/08 , A01H1/04 , C07K14/415 , C12N15/8251
摘要： The present invention relates to a Cucumber plant ( Cucumis sativus ) which provides fruits having an improved shelf life, wherein said plant comprises a stay green gene providing the improved fruit shelf life, wherein said stay green gene encodes a protein comprising the amino acid sequence as shown in SEQ ID No. 2, or amino acid sequences with more than 90% identity, preferably more than 94% identity, more preferably more than 96% identity, even more preferably more than 98% identity; and wherein the expression of said stay green gene is reduced as compared to the expression of said stay green gene in a cucumber plant not providing fruits having an improved shelf life or the enzymatic activity of said protein is reduced as compared to the enzymatic activity of said protein in a cucumber plant not providing fruits having an improved shelf life.
摘要翻译：本发明涉及提供具有改善的保存期限的水果的黄瓜植物（Cucumis sativus），其中所述植物包含提供改善的水果保质期的保持绿色基因，其中所述保持绿色基因编码包含氨基酸序列的蛋白质或SEQ ID No.2所示的氨基酸序列，或具有多于90％同一性，优选大于94％同一性，更优选大于96％同一性，甚至更优选大于98％同一性的氨基酸序列; 并且其中与不提供具有改善的保质期的水果的黄瓜植物中的所述残留绿色基因的表达相比，所述残留绿色基因的表达减少，或者所述蛋白的酶活性与所述蛋白的酶活性相比降低黄瓜植物中的蛋白质不能提供具有改善的保质期的水果。

3. 发明申请

WO2014100289A1 TRANSCRIPTIONAL REGULATION FOR IMPROVED PLANT PRODUCTIVITY 审中-公开
标题翻译：改良植物生产力的转录规定
公开(公告)号：WO2014100289A1
公开(公告)日：2014-06-26
申请号：PCT/US2013/076308
申请日：2013-12-18
申请人： METABOLIX, INC.
发明人： AMBAVARAM, Madana M.R. , SOMLEVA, Mariya
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8205 , C12N15/8207 , C12N15/8223 , C12N15/8225 , C12N15/8226 , C12N15/8241 , C12N15/8245 , C12N15/8251 , C12N15/8271 , C12N15/8273 , Y02A40/146
摘要： Methods comprising DNA constructs and polynucleotides of functional transcription factors for improving photosynthetic capacity, biomass and/or grain yield and stress tolerance in various crop and model plants, dicots and monocots with the C 3 or C 4 photosynthetic pathways are described herein.
摘要翻译：本文描述了包含用于改善各种作物和模型植物，双歧杆菌和具有C3或C4光合途径的单子叶植物的光合能力，生物量和/或谷粒产量和胁迫耐受性的功能转录因子的DNA构建体和多核苷酸的方法。

4. 发明申请

WO2014081729A1 TAL-MEDIATED TRANSFER DNA INSERTION 审中-公开
标题翻译： TAL介导转移DNA插入
公开(公告)号：WO2014081729A1
公开(公告)日：2014-05-30
申请号：PCT/US2013/070815
申请日：2013-11-19
申请人： J.R. SIMPLOT COMPANY
发明人： ROMMENS, Caius M. , DUAN, Hui , WEEKS, Troy J.
IPC分类号： C12N15/82
CPC分类号： C12N15/8213 , A23L19/18 , A23V2002/00 , C12N15/8209 , C12N15/8245 , C12N15/825 , C12N15/8251 , C12N15/8274 , C12N15/8278 , C12N15/8282
摘要： The invention relates to methods for stably integrating a desired polynucleotide into a plant genome, comprising transforming plant material with a first vector comprising nucleotide sequences encoding TAL proteins designed to recognize a target sequence; transforming the plant material with a second vector comprising (i) a marker gene that is not operably linked to a promoter ("promoter-free marker cassette") and which comprises a sequence homologous to the target sequence; and (ii) a desired polynucleotide; and identifying transformed plant material in which the desired polynucleotide is stably integrated.
摘要翻译：本发明涉及用于将期望的多核苷酸稳定整合到植物基因组中的方法，包括用包含设计用于识别靶序列的编码TAL蛋白的核苷酸序列的第一载体转化植物材料; 用第二载体转化植物材料，所述第二载体包含（i）不可操作地连接至启动子的标记基因（“无启动子标记盒”），其包含与靶序列同源的序列; 和（ii）所需的多核苷酸; 并鉴定其中期望的多核苷酸稳定整合的转化的植物材料。

5. 发明申请

WO2014025860A1 SOYBEAN CCP1 PROMOTER AND ITS USE IN CONSTITUTIVE EXPRESSION OF TRANSGENIC GENES IN PLANTS 审中-公开
标题翻译：大豆CCP1促进剂及其在植物中转基因表达的组合表达
公开(公告)号：WO2014025860A1
公开(公告)日：2014-02-13
申请号：PCT/US2013/053910
申请日：2013-08-07
申请人： E. I. DU PONT DE NEMOURS AND COMPANY
发明人： LI, Zhongsen
IPC分类号： C12N15/82 , C12N15/29 , A01H5/00
CPC分类号： C12N15/8216 , C12N15/8209 , C12N15/8245 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8273 , C12N15/8274 , C12N15/8279 , C12N15/8286
摘要： The invention relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean copper chaperone homolog gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a constitutive manner in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.
摘要翻译：本发明涉及大豆，特别是大豆铜伴侣同源基因及其片段的启动子的基因表达调控序列及其在植物中以组成型促进一种或多种异源核酸片段的表达的用途。本发明还公开了组合物，多核苷酸构建体，转化的宿主细胞，转基因植物和含有重组构建体的启动子的种子，以及制备和使用该重组构建体的方法。

6. 发明申请

WO2013148329A1 CHARGED NUTRITIVE PROTEINS AND METHODS 审中-公开
标题翻译：充足的营养蛋白和方法
公开(公告)号：WO2013148329A1
公开(公告)日：2013-10-03
申请号：PCT/US2013/032212
申请日：2013-03-15
申请人： PRONUTRIA, INC.
发明人： BERRY, David, Arthur , BOGHIGIAN, Brett, Adam , SILVER, Nathaniel, W. , VON MALTZAHN, Geoffrey , CHILLAKURU, Rajeev , HAMILL, Michael, J.
IPC分类号： C07K14/435 , A23J3/04 , C12N15/12 , C12N15/63 , C12N15/74
CPC分类号： C07K14/001 , A23J3/04 , A23L33/17 , A23L33/18 , A23L33/30 , A23V2002/00 , A61K31/198 , A61K38/00 , A61K38/16 , C07K14/415 , C12N15/8251 , Y02A50/473 , A23V2200/316 , A23V2250/55 , A61K2300/00
摘要： Charged nutritive proteins are provided. In some embodiments the nutritive proteins an aqueous solubility of at least 12.5 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 50 g/L at pH 7. In some embodiments the nutritive proteins an aqueous solubility of at least 100 g/L at pH 7. In some embodiments the nutritive proteins comprise at least one of a level of a) a ratio of branch chain amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of branch chain amino acid residues to total amino acid residues present in a benchmark protein; b) a ratio of leucine residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of leucine residues to total amino acid residues present in a benchmark protein; and c) a ratio of essential amino acid residues to total amino acid residues present in the nutritive protein equal to or greater than the ratio of essential amino acid residues to total amino acid residues present in a benchmark protein. Also provided are nucleic acids encoding the proteins, recombinant microorganisms that make the proteins, methods of making the proteins using recombinant microorganisms, compositions that comprise the proteins, and methods of using the proteins, among other things.
摘要翻译：提供带电的营养蛋白质。在一些实施方案中，营养蛋白质在pH7下的水溶解度至少为12.5g / L。在一些实施方案中，营养蛋白质在pH7下具有至少50g / L的水溶解度。在一些实施方案中，营养蛋白质的水溶解度为在一些实施方案中，营养蛋白质包含以下中的至少一种：a）支链氨基酸残基与存在于营养蛋白中的总氨基酸残基的比例等于或大于支链氨基酸残基与基准蛋白中存在的总氨基酸残基的比例; b）营养蛋白中存在的亮氨酸残基与总氨基酸残基的比例等于或大于存在于基准蛋白中的亮氨酸残基与总氨基酸残基的比例; 和c）营养蛋白中存在的必需氨基酸残基与总氨基酸残基的比例等于或大于基本蛋白质中存在的必需氨基酸残基与总氨基酸残基的比例。还提供了编码蛋白质的核酸，制备蛋白质的重组微生物，使用重组微生物制备蛋白质的方法，包含蛋白质的组合物，以及使用蛋白质的方法等。

7. 发明申请

WO2013140155A1 TRANSGENIC PLANTS WITH ALTERED NITRATE LEVELS IN LEAVES 审中-公开
标题翻译：具有改变的硝酸盐水平的转基因植物在叶中
公开(公告)号：WO2013140155A1
公开(公告)日：2013-09-26
申请号：PCT/GB2013/050708
申请日：2013-03-19
申请人： BRITISH AMERICAL TOBACCO (INVESTMENTS) LIMITED
发明人： DAVENPORT, Susan , LE LAY, Pascaline , SANCHEZ TAMBURRINO, Juan Pablo
IPC分类号： C12N15/82
CPC分类号： C12N15/8243 , A24B13/00 , A24D1/00 , C07K14/415 , C12N15/8216 , C12N15/8251
摘要： The present invention relates to genetic constructs, which can be used in the preparation of transgenic plants. The constructs can have the ability of reducing nitrate concentration in the plant, in particular the plant's leaves, and for inducing a senescence-like phenotype. The invention extends to plant cells transformed with such constructs, and to the transgenic plants themselves. The invention also relates to methods of producing transgenic plants, and to methods of reducing nitrate content in plants. The invention also relates to harvested plant leaves, for example tobacco leaves, that have been transformed with the genetic constructs, and to various tobacco articles, such as smoking articles, comprising such harvested plant leaves.
摘要翻译：本发明涉及可用于制备转基因植物的遗传构建体。该构建体可以具有减少植物中的硝酸盐浓度的能力，特别是植物的叶子，并诱导衰老样表型。本发明延伸到用这种构建体转化的植物细胞，并且转移到转基因植物本身。本发明还涉及生产转基因植物的方法以及减少植物中硝酸盐含量的方法。本发明还涉及已经用遗传构建体转化的收获的植物叶，例如烟叶，以及包含这种收获的植物叶的各种烟草制品，例如吸烟制品。

8. 发明申请

WO2013140154A1 TRANSGENIC PLANTS HAVING LOWER NITRATE CONTENT IN LEAVES 审中-公开
标题翻译：具有下降硝酸盐含量的转基因植物
公开(公告)号：WO2013140154A1
公开(公告)日：2013-09-26
申请号：PCT/GB2013/050707
申请日：2013-03-19
申请人： BRITISH AMERICAN TOBACCO (INVESTMENTS) LIMITED
发明人： LE LAY, Pascaline , SANCHEZ TAMBURRINO, Juan-Pablo , DAVENPORT, Susan
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C12N15/8243 , A24B13/00 , A24B15/20 , A24D1/00 , C07K14/415 , C12N15/8251
摘要： The present invention relates to genetic constructs, which can be used in the preparation of transgenic plants. The constructs can have the ability of reducing nitrate concentration in the plant, in particular the plant's leaves, and for inducing a senescence-like phenotype. The invention extends to plant cells transformed with such constructs, and to the transgenic plants themselves. The invention also relates to methods of producing transgenic plants, and to methods of reducing nitrate content in plants. The invention also relates to harvested plant leaves, for example tobacco leaves, that have been transformed with the genetic constructs, and to various tobacco articles, such as smoking articles, comprising such harvested plant leaves.
摘要翻译：本发明涉及可用于制备转基因植物的遗传构建体。该构建体可以具有减少植物中的硝酸盐浓度的能力，特别是植物的叶子，并诱导衰老样表型。本发明延伸到用这种构建体转化的植物细胞，并且转移到转基因植物本身。本发明还涉及生产转基因植物的方法以及减少植物中硝酸盐含量的方法。本发明还涉及已经用遗传构建体转化的收获的植物叶，例如烟叶，以及包含这种收获的植物叶的各种烟草制品，例如吸烟制品。

9. 发明申请

WO2013057507A2 METHODS FOR IMPROVING ABIOTIC STRESS RESPONSE 审中-公开
标题翻译：改善生物应激反应的方法
公开(公告)号：WO2013057507A2
公开(公告)日：2013-04-25
申请号：PCT/GB2012/052598
申请日：2012-10-19
申请人： THE UNIVERSITY OF NOTTINGHAM , THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , THE UNIVERSITY OF SHEFFIELD
发明人： GRAY, Julie , HOLDSWORTH, Michael J , BAILEY-SERRES, Julia
IPC分类号： C12N15/82
CPC分类号： C12N15/8271 , C07K14/415 , C12N15/8251 , C12N15/8261 , C12N15/8267 , C12N15/8273
摘要： Provided are methods and compositions for improving the growth characteristics of plants.
摘要翻译：提供了用于改善植物生长特性的方法和组合物。

10. 发明申请

WO2013040005A1 METHODS AND COMPOSITIONS FOR WEED CONTROL 审中-公开
标题翻译：用于控制的方法和组合物
公开(公告)号：WO2013040005A1
公开(公告)日：2013-03-21
申请号：PCT/US2012/054814
申请日：2012-09-12
申请人： MONSANTO TECHNOLOGY LLC , ADER, Daniel , FINNESSY, John, J. , LI, Zhaolong , SHAH, Ronak, Hasmukh , TAO, Nengbing , TAYLOR, Christina, Marie , TAYLOR, Jennifer, Chou , WANG, Dafu , WEAVER, Lisa, Marie
发明人： ADER, Daniel , FINNESSY, John, J. , LI, Zhaolong , SHAH, Ronak, Hasmukh , TAO, Nengbing , TAYLOR, Christina, Marie , TAYLOR, Jennifer, Chou , WANG, Dafu , WEAVER, Lisa, Marie
IPC分类号： C12N15/82
CPC分类号： A01H3/04 , A01N43/80 , C07H21/04 , C12N9/1092 , C12N9/88 , C12N15/8218 , C12N15/8251 , C12N15/8275 , C12N15/8278
摘要： The present invention provides novel compositions for use to enhance weed control. Specifically, the present invention provides for methods and compositions that modulate acetolactate synthase in weed species. The present invention also provides for combinations of compositions and methods that enhance weed control.
摘要翻译：本发明提供用于增强杂草控制的新型组合物。具体地说，本发明提供了在杂草种中调节乙酰乳酸合酶的方法和组合物。本发明还提供组合物和增强杂草控制的方法的组合。

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