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1. 发明申请

WO2008028143A3 BIOREACTOR FOR GROWING BIOLOGICAL MATERIALS SUPPORTED ON A LIQUID SURFACE 审中-公开
标题翻译：生物反应器用于生长液体表面所支持的生物材料
公开(公告)号：WO2008028143A3
公开(公告)日：2008-04-17
申请号：PCT/US2007077414
申请日：2007-08-31
申请人： BIOLEX INC , EVERETT KEITH , JOHNSTON EUGENE
发明人： EVERETT KEITH , JOHNSTON EUGENE
IPC分类号： C12M1/00
CPC分类号： C12M23/48 , C12M21/02 , C12M23/14 , C12M23/24 , C12M23/44
摘要： A bioreactor assembly of the present invention is provided for holding a media and supporting growth of a plurality of plants. The assembly includes a light source and a container having a light transmissive wall structure and defining a reservoir. A major axis of the reservoir is substantially horizontal allowing the reservoir to be filled with media to a partial level and to define a relatively large surface area for support of the plants. The assembly may also include clamps to secure and seal separate wall structure portions of the container together, and end caps to the wall portions, to define an aseptic environment. As another option, the clamps may define openings therethrough that allow passage of various devices for measuring and controlling bioreactor function such as a gas supply nozzle, a gas exit nozzle, an air temperature probe, a pH probe, a sampling drain, a gas composition probe and a media temperature probe.
摘要翻译：提供本发明的生物反应器组件用于保持培养基并支持多种植物的生长。该组件包括光源和具有透光壁结构并限定储存器的容器。储存器的主轴线基本上是水平的，从而允许储存器填充介质至部分水平并且限定用于支撑植物的相对较大的表面区域。组件还可以包括夹具，以将容器的分离的壁结构部分固定和密封在一起，并且将端盖固定到壁部分，以限定无菌环境。作为另一种选择，夹具可限定贯穿其中的开口，所述开口允许用于测量和控制生物反应器功能的各种装置的通过，诸如气体供应喷嘴，气体出口喷嘴，空气温度探针，pH探针，采样排水管，气体组合物探头和介质温度探头。

2. 发明申请

WO2007084922A2 COMPOSITIONS AND METHODS FOR HUMANIZATION AND OPTIMIZATION OF N-GLYCANS IN PLANTS 审中-公开
标题翻译： N-GLYCANS在植物中的人类化和优化的组合物和方法
公开(公告)号：WO2007084922A2
公开(公告)日：2007-07-26
申请号：PCT/US2007060642
申请日：2007-01-17
申请人： BIOLEX INC , DICKEY LYNN F , COX KEVIN M , PEELE CHARLES G
发明人： DICKEY LYNN F , COX KEVIN M , PEELE CHARLES G
CPC分类号： C07K14/47 , C07K16/00 , C07K2317/13 , C07K2317/41 , C07K2317/71 , C07K2317/72 , C07K2317/732 , C07K2317/734 , C12N15/8257 , C12N15/8258
摘要： Methods for altering the N-glycosylation pattern of proteins in higher plants are provided. The methods comprise introducing into the plant a recombinant construct that provides for the inhibition of expression of al,3-fucosyltransferase (FucT) and ß 1 ,2-xylosyltransferase (XyIT) in a plant. Use of these constructs to inhibit or suppress expression of both of these enzymes, and isoforms thereof, advantageously provides for the production of endogenous and heterologous proteins having a "humanized" N-glycosylation pattern without impacting plant growth and development. Stably transformed higher plants having this protein N-glycosylation pattern are provided. Glycoprotein compositions, including monoclonal antibody compositions, having substantially homogeneous glycosylation profiles, and which are substantially homogeneous for the GO glycoform, are also provided.
摘要翻译：提供了改变高等植物中蛋白质N-糖基化模式的方法。所述方法包括向植物中引入一种重组构建体，其在植物中提供抑制al-3-岩藻糖基转移酶（FucT）和β1-木糖基转移酶（XyIT）的表达。这些构建体用于抑制或抑制这两种酶及其同种型的表达有利地提供具有“人源化”N-糖基化模式的内源和异源蛋白质的生产，而不影响植物的生长和发育。提供了具有这种蛋白质N-糖基化模式的稳定转化的高等植物。还提供了糖蛋白组合物，包括具有基本上均匀的糖基化特征的单克隆抗体组合物，并且对于GO糖型基本均匀。

3. 发明申请

WO2005005643A2 CHLOROPLAST TRANSFORMATION OF DUCKWEED 审中-公开
标题翻译：杜鹃花的叶绿体转化
公开(公告)号：WO2005005643A2
公开(公告)日：2005-01-20
申请号：PCT/US2004/021106
申请日：2004-06-30
申请人： BIOLEX, INC. , COX, Kevin, M. , PEELE, Charles, G.
发明人： COX, Kevin, M. , PEELE, Charles, G.
IPC分类号： C12N15/82
CPC分类号： C12N15/8214
摘要： The present invention provides methods and compositions for the transformation of duckweed plastids. The compositions and methods of the invention are useful in increasing the recombinant protein production capacity of the duckweed expression system. The compositions of the invention include transformed duckweed plastids and transplastomic duckweed cells and plants, as well as nucleic acid constructs useful for transforming duckweed plastids. The invention also provides methods for introducing one or more heterologous nucleotide sequences into a duckweed plastome.
摘要翻译：本发明提供了用于转化浮萍质体的方法和组合物。本发明的组合物和方法可用于增加浮萍表达系统的重组蛋白生产能力。本发明的组合物包括转化的浮萍质体和转质体浮萍细胞和植物，以及用于转化浮萍质体的核酸构建体。本发明还提供了将一种或多种异源核苷酸序列导入浮萍体内的方法。

4. 发明申请

WO2005078109A2 EXPRESSION OF PLASMINOGEN AND MICROPLASMINOGEN IN DUCKWEED 审中-公开
标题翻译：表达胰岛素和微管蛋白在DUCKWEED中的表达
公开(公告)号：WO2005078109A2
公开(公告)日：2005-08-25
申请号：PCT/US2005/004245
申请日：2005-02-11
申请人： BIOLEX, INC. , SPENCER, David , DICKEY, Lynn, F. , GASDASKA, John, R. , WANG, Xiaowei , COX, Kevin, M. , PEELE, Charles, G.
发明人： SPENCER, David , DICKEY, Lynn, F. , GASDASKA, John, R. , WANG, Xiaowei , COX, Kevin, M. , PEELE, Charles, G.
IPC分类号： C12N15/82
CPC分类号： C12N15/8257 , C12N9/6435 , C12Y304/21007
摘要： The present invention provides methods and compositions for the production of recombinant plasminogen, microplasminogen, and fragments thereof in a duckweed expression system. It is the novel finding of the present invention that a duckweed expression system may be used to produce high levels of plasminogen and microplasminogen. The duckweed-produced plasminogen and microplasminogen can be activated to produce a polypeptide having protease activity. Thus, the invention encompasses methods for the expression of plasminogen, microplasminogen, and fragments thereof in duckweed, duckweed plants that are transformed with expression cassettes for the expression of plasminogen, microplasminogen, and fragments thereof, and nucleic acids comprising nucleotide sequences encoding plasminogen, microplasminogen, and fragments thereof, where these nucleotide sequences are modified to enhance their expression in duckweed.
摘要翻译：本发明提供了用于在浮萍表达系统中生产重组纤溶酶原，微量血浆原和其片段的方法和组合物。本发明的新发现是，浮萍表达系统可用于产生高水平的纤溶酶原和微量淀粉样蛋白。可以将浮萍产生的纤溶酶原和微量血浆原活化以产生具有蛋白酶活性的多肽。因此，本发明包括在用于表达纤溶酶原，微量血浆原和其片段的表达盒转化的浮萍，浮萍植物中表达纤溶酶原，微量淀粉样蛋白及其片段的方法，以及包含编码纤溶酶原，微量稀释细胞的核苷酸序列的核酸及其片段，其中这些核苷酸序列被修饰以增强其在浮萍中的表达。

5. 发明申请

WO0210414A2 EXPRESSION OF BIOLOGICALLY ACTIVE POLYPEPTIDES IN DUCKWEED 审中-公开
标题翻译：表达生物活性多聚体在DUCKWEED
公开(公告)号：WO0210414A2
公开(公告)日：2002-02-07
申请号：PCT/US0123400
申请日：2001-07-26
申请人： BIOLEX INC , STOMP ANNE MARIE , DICKEY LYNN , GASDASKA JOHN
发明人： STOMP ANNE-MARIE , DICKEY LYNN , GASDASKA JOHN
IPC分类号： C12N15/09 , C07K14/56 , C12N5/10 , C12N9/00 , C12N15/82 , C12P21/02 , C12P21/08 , C12N15/62 , C12N15/67
CPC分类号： C07K14/56 , C12N15/8216 , C12N15/8257
摘要： Methods, nucleic acid sequences, and transformed duckweed plant or duckweed nodule cultures for the expression and the secretion of biologically active polypeptides from genetically engineered duckweed are provided. Expression of recombinant polypeptides in duckweed is improved by modifying the nucleotide sequence of the expression cassette encoding the polypeptide for improved expression in duckweed. Recovery of biologically active polypeptides from duckweed is improved by linking the biologically active polypeptide to a signal peptide that directs the secretion of the polypeptide into the culture medium.
摘要翻译：提供了用于从遗传工程化的浮萍中表达和分泌生物活性多肽的方法，核酸序列和转化的浮萍植物或浮萍结核菌培养物。通过修饰编码多肽的表达盒的核苷酸序列来改善浮萍中重组多肽的表达，从而改善浮萍中的表达。通过将生物活性多肽与指导多肽分泌到培养基中的信号肽连接来提高从浮萍中回收生物活性多肽。

6. 发明申请

WO2008028143A2 BIOREACTOR FOR GROWING BIOLOGICAL MATERIALS SUPPORTED ON A LIQUID SURFACE 审中-公开
标题翻译：生物反应器用于生长液体表面上支持的生物材料
公开(公告)号：WO2008028143A2
公开(公告)日：2008-03-06
申请号：PCT/US2007/077414
申请日：2007-08-31
申请人： BIOLEX, INC. , EVERETT, Keith , JOHNSTON, Eugene
发明人： EVERETT, Keith , JOHNSTON, Eugene
IPC分类号： C12M1/00
CPC分类号： C12M23/48 , C12M21/02 , C12M23/14 , C12M23/24 , C12M23/44
摘要： A bioreactor assembly of the present invention is provided for holding a media and supporting growth of a plurality of plants. The assembly includes a light source and a container having a light transmissive wall structure and defining a reservoir. A major axis of the reservoir is substantially horizontal allowing the reservoir to be filled with media to a partial level and to define a relatively large surface area for support of the plants. The assembly may also include clamps to secure and seal separate wall structure portions of the container together, and end caps to the wall portions, to define an aseptic environment. As another option, the clamps may define openings therethrough that allow passage of various devices for measuring and controlling bioreactor function such as a gas supply nozzle, a gas exit nozzle, an air temperature probe, a pH probe, a sampling drain, a gas composition probe and a media temperature probe.
摘要翻译：提供本发明的生物反应器组件用于保持介质并支持多个植物的生长。组件包括光源和具有透光壁结构并限定储存器的容器。储存器的主轴基本上是水平的，允许储存器被填充到局部水平的介质并且限定用于支撑植物的相对较大的表面积。组件还可以包括用于将容器的分开的壁结构部分固定并密封在一起的夹具以及端壁到壁部分，以限定无菌环境。作为另一选择，夹具可以限定穿过其中的开口，其允许用于测量和控制生物反应器功能的各种装置的通过，例如气体供应喷嘴，气体出口喷嘴，空气温度探针，pH探针，取样排水管，气体组成探头和介质温度探头。

7. 发明申请

WO2005078109A3 EXPRESSION OF PLASMINOGEN AND MICROPLASMINOGEN IN DUCKWEED 审中-公开
标题翻译：杜氏鲍曼不动杆菌的表达及其微丝体表达
公开(公告)号：WO2005078109A3
公开(公告)日：2005-11-10
申请号：PCT/US2005004245
申请日：2005-02-11
申请人： BIOLEX INC , SPENCER DAVID , DICKEY LYNN F , GASDASKA JOHN R , WANG XIAOWEI , COX KEVIN M , PEELE CHARLES G
发明人： SPENCER DAVID , DICKEY LYNN F , GASDASKA JOHN R , WANG XIAOWEI , COX KEVIN M , PEELE CHARLES G
IPC分类号： A01H1/00 , C12N9/68 , C12N15/82
CPC分类号： C12N15/8257 , C12N9/6435 , C12Y304/21007
摘要： The present invention provides methods and compositions for the production of recombinant plasminogen, microplasminogen, and fragments thereof in a duckweed expression system. It is the novel finding of the present invention that a duckweed expression system may be used to produce high levels of plasminogen and microplasminogen. The duckweed-produced plasminogen and microplasminogen can be activated to produce a polypeptide having protease activity. Thus, the invention encompasses methods for the expression of plasminogen, microplasminogen, and fragments thereof in duckweed, duckweed plants that are transformed with expression cassettes for the expression of plasminogen, microplasminogen, and fragments thereof, and nucleic acids comprising nucleotide sequences encoding plasminogen, microplasminogen, and fragments thereof, where these nucleotide sequences are modified to enhance their expression in duckweed.
摘要翻译：本发明提供了用于在浮萍表达系统中生产重组纤溶酶原，微纤溶酶原及其片段的方法和组合物。本发明的新发现是浮萍表达系统可用于产生高水平的纤溶酶原和微纤溶酶原。浮萍产生的纤溶酶原和微纤溶酶原可被活化以产生具有蛋白酶活性的多肽。因此，本发明包括用于在表达纤溶酶原，微纤溶酶原及其片段的表达盒和包含编码纤溶酶原，微纤溶酶原的核苷酸序列的核酸的浮萍，浮萍植物中表达纤溶酶原，微纤溶酶原及其片段的方法及其片段，其中这些核苷酸序列被修饰以增强它们在浮萍中的表达。

8. 发明申请

WO2005035767A1 ALPHA INTERFERON VARIANTS 审中-公开
标题翻译：阿尔法干扰素
公开(公告)号：WO2005035767A1
公开(公告)日：2005-04-21
申请号：PCT/US2004/011965
申请日：2004-04-16
申请人： BIOLEX, INC. , DICKEY, Lynn , GASDASKA, John , COX, Kevin
发明人： DICKEY, Lynn , GASDASKA, John , COX, Kevin
IPC分类号： C12N15/82
CPC分类号： C12N15/8216 , C07K14/56 , C07K2317/55 , C12N15/8257
摘要： The present invention provides biologically active variants of human α-2b- interferon. The variants contain carboxy terminus truncations when compared with the amino acid sequence of full-length human α-2b-interferon. It is the novel finding of the present invention that these truncated variants have the biological activity of full-length human α-2b-interferon. The invention encompasses these biologically active variant α-interferons, as well as polynucleotides encoding these interferons. Expression cassettes comprising these polynucleotides and host cells comprising the expression cassettes are also provided. The invention also provides compositions comprising variant α-interferon polypeptides and a pharmaceutically acceptable carrier.
摘要翻译：本发明提供人α-2b-干扰素的生物活性变体。当与全长人α-2b-干扰素的氨基酸序列相比时，变体含有羧基末端截短。本发明的新发现是这些截短的变体具有全长人α-2b-干扰素的生物学活性。本发明包括这些生物活性变体α-干扰素，以及编码这些干扰素的多核苷酸。还提供了包含这些多核苷酸的表达盒和包含表达盒的宿主细胞。本发明还提供包含变体α-干扰素多肽和药学上可接受的载体的组合物。

9. 发明申请

WO2004108881A3 BIOREACTOR FOR GROWING BIOLOGICAL MATERIALS SUPPORTED ON A LIQUID SURFACE 审中-公开
标题翻译：生物反应器，用于生长液体表面上支持的生物材料
公开(公告)号：WO2004108881A3
公开(公告)日：2005-03-03
申请号：PCT/US2004016437
申请日：2004-05-25
申请人： BIOLEX INC , BRANSON R EDWARD , EVERETT KEITH , HESTER BOB , VICKERS TIMOTHY B
发明人： BRANSON R EDWARD , EVERETT KEITH , HESTER BOB , VICKERS TIMOTHY B
IPC分类号： C12M1/00
CPC分类号： C12M21/02 , C12M23/14 , C12M23/24 , C12M23/44 , C12M23/48 , Y10S47/06
摘要： A bioreactor assembly (10) of the present invention for holding a media and supporting growth of a plurality of plants. The assembly includes a light source (13) and a container (11)having a light transmissive wall structure and defining a reservoir. A major axis of the reservoir is substantially horizontal allowing the reservoir to be filled with media to a partial level and to define a relatively large surface area for support of the plants. The assembly may also include clamps (40) to secure and seal separate wall structure portions of the container together, and end caps to the wall portions, to define an aseptic environment. As another option, the clamps may define openings therethrough that allow passage of various devices for measuring and controlling bioreactor function such as a gas supply nozzle, a gas exit nozzle, an air temperature probe, a pH probe, a sampling drain, a gas composition probe and a media temperature probe.
摘要翻译：本发明的生物反应器组件（10），用于保持介质并支持多个植物的生长。组件包括光源（13）和具有透光壁结构并限定储存器的容器（11）。储存器的长轴基本上是水平的，允许储存器被填充到局部水平的介质并且限定用于支撑植物的相对大的表面积。组件还可以包括用于将容器的分离的壁结构部分固定并密封在一起的端盖（40），并将端盖封闭到壁部分，以限定无菌环境。作为另一选择，夹具可以限定穿过其中的开口，其允许用于测量和控制生物反应器功能的各种装置的通过，例如气体供应喷嘴，气体出口喷嘴，空气温度探针，pH探针，取样排水口，气体组成探头和介质温度探头。

10. 发明申请

WO0210414A9 EXPRESSION OF BIOLOGICALLY ACTIVE POLYPEPTIDES IN DUCKWEED 审中-公开
标题翻译：表达生物活性多聚体在DUCKWEED
公开(公告)号：WO0210414A9
公开(公告)日：2003-10-30
申请号：PCT/US0123400
申请日：2001-07-26
申请人： BIOLEX INC , STOMP ANNE-MARIE , DICKEY LYNN , GASDASKA JOHN
发明人： STOMP ANNE-MARIE , DICKEY LYNN , GASDASKA JOHN
IPC分类号： C12N15/09 , C07K14/56 , C12N5/10 , C12N9/00 , C12N15/82 , C12P21/02 , C12P21/08 , C12N15/62 , C12N15/67
CPC分类号： C07K14/56 , C12N15/8216 , C12N15/8257
摘要： Methods, nucleic acid sequences, and transformed duckweed plant or duckweed nodule cultures for the expression and the secretion of biologically active polypeptides from genetically engineered duckweed are provided. Expression of recombinant polypeptides in duckweed is improved by modifying the nucleotide sequence of the expression cassette encoding the polypeptide for improved expression in duckweed. Recovery of biologically active polypeptides from duckweed is improved by linking the biologically active polypeptide to a signal peptide that directs the secretion of the polypeptide into the culture medium.
摘要翻译：提供了用于从遗传工程化的浮萍中表达和分泌生物活性多肽的方法，核酸序列和转化的浮萍植物或浮萍结核菌培养物。通过修饰编码多肽的表达盒的核苷酸序列来改善浮萍中重组多肽的表达，从而改善浮萍中的表达。通过将生物活性多肽与指导多肽分泌到培养基中的信号肽连接来提高从浮萍中回收生物活性多肽。

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