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61. 发明申请

WO2004031351A9 MICROARRAYS HAVING MULTIPLE OLIGONUCLEOTIDES IN SINGLE ARRAY FEATURES 审中-公开
标题翻译：在单阵列特征中具有多个寡核苷酸的微阵列
公开(公告)号：WO2004031351A9
公开(公告)日：2004-05-21
申请号：PCT/US0330725
申请日：2003-09-30
申请人： NIMBLEGEN SYSTEMS INC
发明人： ALBERT THOMAS , NORTON JASON , GREEN ROLAND
IPC分类号： C07H21/00 , C07H21/04 , C12M1/34 , C12N20060101 , C12N15/11 , C12Q1/68 , C12N
CPC分类号： B82Y30/00 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00675 , B01J2219/00711 , B01J2219/00722 , C07H21/00 , C12Q1/6837 , C40B40/06 , Y02P20/55 , C12Q2565/513 , C12Q2565/543 , C12Q2537/143
摘要： The present invention is a method for synthesizing microarrays having different oligonucleotides present within one feature area of the array. The method utilizes the techniques common to microarray synthesis, but limits the duration in which selected feature areas on the array are initially dosed with light so as to only deprotect a calculated ratio of the compounds forming the array's binding layer. The compounds initially deprotected are capped with a nonphotosensitive protecting group, such as di-methoxy-trityl, to inhibit their involvement in the synthesis of a first group of DNA strands built onto the array. Once the first group of DNA strands have been synthesized, the original deprotected group may then be further processed to build one or more groups of DNA strands in the same feature area as the first group of DNA strands. The present invention also includes microarrays manufactured using the method.
摘要翻译：本发明是用于合成具有存在于阵列的一个特征区域内的不同寡核苷酸的微阵列的方法。该方法利用了微阵列合成常用的技术，但是限制了阵列上选择的特征区域首先用光投射的持续时间，以便仅对形成阵列结合层的化合物的计算比例进行去保护。最初去保护的化合物用非光敏保护基如二甲氧基三苯甲基封端，以抑制它们参与构建到阵列上的第一组DNA链的合成。一旦第一组DNA链合成完成，原来的脱保护基就可以进一步加工，在与第一组DNA链相同的特征区域内构建一组或多组DNA链。本发明还包括使用该方法制造的微阵列。

62. 发明申请

WO03036265A8 ASSAY SYSTEMS WITH ADJUSTABLE FLUID COMMUNICATION 审中-公开
标题翻译：具有可调流体通信的分析系统
公开(公告)号：WO03036265A8
公开(公告)日：2004-05-21
申请号：PCT/US0234699
申请日：2002-10-28
申请人： VIRTUAL ARRAYS INC , RAVKIN ILYA , BESKE OREN E
发明人： RAVKIN ILYA , BESKE OREN E
IPC分类号： B01L3/00 , B01L9/00 , C12M1/22 , C12M1/34 , C40B40/06 , C40B40/10 , C40B60/14 , C40B70/00 , G01N
CPC分类号： B82Y30/00 , B01J2219/00315 , B01J2219/00317 , B01J2219/0036 , B01J2219/00364 , B01J2219/00511 , B01J2219/0054 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00617 , B01J2219/00621 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00662 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01J2219/0074 , B01J2219/00743 , B01L3/5085 , B01L9/523 , B01L2200/0642 , B01L2300/06 , B01L2300/0829 , B01L2300/0851 , B01L2300/0861 , B01L2300/168 , C12M23/12 , C40B40/06 , C40B40/10 , C40B60/14 , C40B70/00
摘要： Systems, including apparatus and methods, for performing assays in which two or more samples may be held in or out of simultaneous contact with the same fluid environment.
摘要翻译：用于执行测定的系统，包括设备和方法，其中两个或更多个样品可保持在同一流体环境中或与同一流体环境同时接触。

63. 发明申请

WO2004018623A3 SUBSTRATES FOR ISOLATING, REACTING AND MICROSCOPICALLY ANALYZING MATERIALS 审中-公开
标题翻译：分离，反应和微观分析材料的基材
公开(公告)号：WO2004018623A3
公开(公告)日：2004-04-15
申请号：PCT/US0325685
申请日：2003-08-18
申请人： CLINICAL MICROARRAYS INC , MONTAGU JEAN I , DOWD ROGER , ROOT DAVID
发明人： MONTAGU JEAN I , DOWD ROGER , ROOT DAVID
IPC分类号： G01N1/28 , C40B40/06 , C40B40/10 , C40B60/14 , G01N21/64 , G01N33/58 , G01N37/00 , G01N33/543
CPC分类号： G01N21/6452 , B01J2219/00315 , B01J2219/00387 , B01J2219/00533 , B01J2219/00605 , B01J2219/00612 , B01J2219/00637 , B01J2219/00641 , B01J2219/00722 , B01J2219/00725 , B01J2219/0074 , C40B40/06 , C40B40/10 , C40B60/14 , G01N21/274 , G01N21/6428 , G01N21/6456 , G01N33/54393 , G01N33/582
摘要： An immobilizing device for biological material comprises a rigid support (12) carrying a substrate layer (20, 20') of polymer having biological immobilizing properties, e.g. for amino and nucleic acids. Substantially solid ultra-thin substrate layers (20') having a thickness less than about 5 micron, preferably between about 0.1 and 0.5 micron, and micro-porous, ultra-thin substrate layers (20') having a thickness less than about 5 micron, preferably less than 3 micron, 2 or 1 micron are shown, which may be segmented by isolating moats M. The substrate layer is on a microscope slide (302), round disc (122), bio-cassette, at the bottom of a well of a multiwell plate, and as a coating inside a tube. Fluorescence or luminescence intensity and geometric calibration spots (420) are shown. Reading is enhanced by the intensity calibration spots (420) to enable normalization of readings under uneven illumination conditions, as when reading by dark field, side illumination mode. The reference spots are shown being printed simultaneously with printing an array of biological spots or with the same equipment. Methods of forming layers of the device include controlled drawing from a bath of coating composition and drying, and spinning of C-D shaped substrates. Post-forming treatment is shown by corona treatment and radiation. Adherent metal oxides (14), silica-based materials and other materials are used to unite layers of the composite. In multiwell plates the oxide promotes joining of a bottom plate (95, 95') and upper, well-defining structure (94) of dissimilar material. The oxides (14) also provide beneficial opacity to prevent light entering the glass support, for applying potential to the substrate, etc.
摘要翻译：用于生物材料的固定装置包括承载具有生物固定性质的聚合物的基底层（20,20'）的刚性支撑体（12），例如。用于氨基和核酸。具有厚度小于约5微米，优选约0.1至0.5微米的基本上固体的超薄基底层（20'）和厚度小于约5微米的微孔超薄基底层（20'），优选小于3微米，2或1微米，其可以通过隔离护墙M分段。基底层在显微镜载玻片（302），圆盘（122），生物盒，底部多孔板，以及管内的涂层。显示荧光或发光强度和几何校准点（420）。通过强度校准点（420）来增强读数，以使得在不均匀照射条件下的读数正常化，如通过暗场，侧面照明模式读取。参考点被显示为同时印刷生物斑点阵列或用相同的设备打印。形成装置层的方法包括从涂料组合物的浴中进行控制拉伸和干燥，以及C-D形基材的纺丝。后处理通过电晕处理和辐射显示。使用粘附金属氧化物（14），二氧化硅基材料和其他材料来组合复合材料层。在多孔板中，氧化物促进底板（95,95'）和不同材料的上部，良好限定结构（94）的接合。氧化物（14）还提供有益的不透明性，以防止进入玻璃载体的光，将电位施加到基底等。

64. 发明申请

WO2004022701A2 EXPONENTIAL AMPLIFICATION OF NUCLEIC ACIDS USING NICKING AGENTS 审中-公开
标题翻译：用镍试剂诱导核酸的指数扩增
公开(公告)号：WO2004022701A2
公开(公告)日：2004-03-18
申请号：PCT/US2002/022677
申请日：2002-07-15
申请人： KECK GRADUATE INSTITUTE , VAN NESS, Jeffrey , GALAS, David, J. , VAN NESS, Lori, K.
发明人： VAN NESS, Jeffrey , GALAS, David, J. , VAN NESS, Lori, K.
IPC分类号： C12N
CPC分类号： C12Q1/6809 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/00722 , C12Q1/6844 , C12Q2600/156 , C12Q2600/158 , C40B40/06 , C12Q2525/131 , C12Q2521/313 , C12Q2565/501 , C12Q2527/107 , C12Q2525/155 , C12Q2521/301 , C12Q2531/119 , C12Q2521/513
摘要： The present invention provides methods and compositions for exponential amplification of nucleic acid molecules using nicking agents. In certain aspects, the amplification may be performed isothermally. This invention is useful in many areas such as disease diagnosis.
摘要翻译：本发明提供了使用切刻剂指数扩增核酸分子的方法和组合物。在某些方面，可以等温地进行扩增。本发明在诸如疾病诊断等许多领域是有用的。

65. 发明申请

WO03076076B1 METHOD FOR APPLYING A SUBSTANCE TO A SUBSTRATE 审中-公开
标题翻译：将物质施到基材上
公开(公告)号：WO03076076B1
公开(公告)日：2004-03-18
申请号：PCT/EP0302477
申请日：2003-03-11
申请人： AXARON BIOSCIENCE AG , MUELLER RALPH
发明人： MUELLER RALPH
IPC分类号： B01L3/02 , B05D1/26 , C40B40/06 , C40B60/14 , H01L51/00 , H01L51/40 , B41J2/00 , H01L27/00 , H01L51/20
CPC分类号： H01L51/0003 , B01J2219/00274 , B01J2219/00367 , B01J2219/00369 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00659 , B01J2219/00662 , B01J2219/00677 , B01J2219/00691 , B01J2219/00722 , B01L3/0244 , B01L3/0262 , B01L2300/0809 , B01L2300/0838 , B05D1/26 , B82Y30/00 , C40B40/06 , C40B60/14 , H01L51/0005
摘要： According to the invention, a fine capillary tube (10) is filled with the substance (14). The lower surface (22) of the capillary tube touches the substrate (20) at a defined speed and the substance, which forms a suspended meniscus (18) beyond the lower surface of the capillary tube, wets the substrate. The substance spreads out between the lower surface of the capillary tube and the substrate, propelled by the capillary force. A spot with a shape and size that correspond to the lower surface of the capillary tube is thus formed on the substrate. This permits extremely uniform arrays of small, shape-defining spots to be applied to a substrate. The spotting method is suitable for the construction of organic displays and biochips.
摘要翻译：薄的毛细管（10）填充有材料（14）。所述Kapillarendfläche（22）在给定的速度接触时，基板（20）。其特征在于润湿的织物上形成挂弯月面（18）超出Kapillarendfläche，基板。 Kapillarendfläche与基板之间，该材料价差 - 通过毛细力驱动的 - 从。在基板上，从而形成一个光点的Kapillarendfläche的尺寸和形状。以这种方式的小斑点非常均匀的阵列定义的形状可以被施加到基底上。点样方法适用于有机显示器和生物芯片的发展。

66. 发明申请

WO2004020450A1 METHODS FOR THE CHEMICAL AND PHYSICAL MODIFICATION OF NANOTUBES, METHODS FOR LINKING THE NANOTUBES, METHODS FOR THE DIRECTED POSITIONING OF NANOTUBES, AND USES THEREOF 审中-公开
标题翻译：纳米管的化学和物理修饰方法，用于连接纳米粒子的方法，用于定向取代纳米粒子的方法及其用途
公开(公告)号：WO2004020450A1
公开(公告)日：2004-03-11
申请号：PCT/AU2003/001118
申请日：2003-08-29
申请人： COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION , MCCALL, Maxine , MOGHADDAM, Minoo
发明人： MCCALL, Maxine , MOGHADDAM, Minoo
IPC分类号： C07H21/04
CPC分类号： B82Y30/00 , B01J2219/00524 , B01J2219/00608 , B01J2219/00659 , B01J2219/00668 , B01J2219/00722 , B82Y5/00 , C07H21/04 , C12Q1/6825 , C40B40/06 , C12Q2563/116 , C12Q2563/155
摘要： The invention relates to methods for chemically and physically modifying nanotubes with nucleic acid, and uses thereof. In particular, the invention relates to one or more nanotube(s) with one or more nucleic acid molecule(s) attached thereto.
摘要翻译：本发明涉及用核酸对化学和物理修饰纳米管的方法及其应用。特别地，本发明涉及一种或多种与其上附着的一个或多个核酸分子的纳米管。

67. 发明申请

WO2004000471A1 MICROSTAMPING ACTIVATED POLYMER SURFACES 审中-公开
标题翻译：微量激活聚合物表面
公开(公告)号：WO2004000471A1
公开(公告)日：2003-12-31
申请号：PCT/US2003/018487
申请日：2003-06-12
申请人： DUKE UNIVERSITY , CHILKOTI, Ashutosh , YANG, Zhongping , HYUN, Jinho
发明人： CHILKOTI, Ashutosh , YANG, Zhongping , HYUN, Jinho
IPC分类号： B05D1/00
CPC分类号： B82Y30/00 , A61L27/34 , A61L27/50 , A61L27/54 , A61L2300/22 , A61L2300/23 , A61L2300/252 , A61L2300/258 , A61L2300/604 , B01J19/0046 , B01J2219/00382 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00626 , B01J2219/0063 , B01J2219/00635 , B01J2219/00659 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B05D1/185 , B05D1/283 , B82Y40/00 , C07B2200/11 , C40B40/06 , C40B40/10 , C40B60/14 , G01N33/545 , Y10T156/19
摘要： Methods of attaching a ligand to a surface are described that include contacting a surface having an amphiphilic comb polymer present thereon and having a first reactive moiety attached thereto with a substrate having at least one ligand thereon. The ligand can include a second reactive moiety, wherein the second reactive moiety of the biological ligand and the first reactive moiety of the amphiphilic comb polymer form a covalent bond. The substrate can be separated from the surface, thereby leaving the biological ligand covalently bound to the amphiphilic comb polymer.
摘要翻译：描述了将配体连接到表面的方法，其包括使具有其上存在的两亲性梳状聚合物的表面与其上具有至少一个配体的底物接触，并具有与其连接的第一反应性部分。配体可以包括第二反应性部分，其中生物配体的第二反应性部分和两亲梳状聚合物的第一反应性部分形成共价键。底物可以与表面分离，从而使生物配体与两亲性梳状聚合物共价结合。

68. 发明申请

WO0242775A3 CLINICALLY INTELLIGENT DIAGNOSTIC DEVICES AND METHODS 审中-公开
标题翻译：临床智能诊断装置及方法
公开(公告)号：WO0242775A3
公开(公告)日：2003-12-31
申请号：PCT/US0144868
申请日：2001-11-27
申请人： GENEVENTION L L C , JACOBS ALICE ANNE , NIKOLIC BORIS , GUPTA VINEET
发明人： JACOBS ALICE ANNE , NIKOLIC BORIS , GUPTA VINEET
IPC分类号： B01F13/00 , B01F13/08 , B01J19/00 , B01L3/00 , B81B1/00 , C12Q1/68 , C40B40/06 , C40B40/10 , C40B40/12 , C40B40/18 , C40B60/14 , C40B70/00 , G01N33/15 , G01N33/50 , G01N33/53 , G01N33/543 , G01N33/545 , G01N33/547 , G01N33/551 , G01N33/552 , G01N33/553 , G01N37/00 , G01N33/569
CPC分类号： B01F13/0088 , B01F13/08 , B01F13/0809 , B01J19/0046 , B01J2219/00315 , B01J2219/00317 , B01J2219/00481 , B01J2219/00497 , B01J2219/00509 , B01J2219/00511 , B01J2219/00527 , B01J2219/00547 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/00662 , B01J2219/00664 , B01J2219/00677 , B01J2219/00689 , B01J2219/00702 , B01J2219/0072 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B01J2219/0074 , B01J2219/00745 , B01L3/5027 , B01L3/50273 , B01L3/5085 , B01L3/50857 , B01L3/563 , B01L2300/021 , B01L2300/0636 , B01L2300/069 , B01L2300/0819 , B01L2300/0864 , B82Y30/00 , C12Q1/6837 , C40B40/06 , C40B40/10 , C40B40/12 , C40B40/18 , C40B60/14 , C40B70/00 , G01N33/53 , G01N33/543 , G01N33/54366
摘要： The invention relates to the clinically intelligent design of diagnostic devices (such as microarrays) and methods of making and using such devices in differential diagnoses of specific clinical symptoms or sets of symptoms. In one aspect, the devices include various probes used to perform parallel screening of a number of analytes. The probes are clustered on the devices based on known clinical presentations of symptoms associated with specific diseases and disorders.
摘要翻译：本发明涉及诊断装置（例如微阵列）的临床智能设计以及制造和使用这些装置在特定临床症状或症状组的鉴别诊断中的方法。在一个方面，所述装置包括用于执行多个分析物的并行筛选的各种探针。基于与特定疾病和病症相关的症状的已知临床表现，将探针聚集在装置上。

69. 发明申请

WO2003106033A1 REACTION CHAMBER 审中-公开
标题翻译：反应室
公开(公告)号：WO2003106033A1
公开(公告)日：2003-12-24
申请号：PCT/IB2003/002518
申请日：2003-06-10
申请人： MILLENIUM BIOLOGIX AG , ORAM, Guy , BRUNNER, Andreas , HAGG, Rupert , TOMMASINI, Roberto
发明人： ORAM, Guy , BRUNNER, Andreas , HAGG, Rupert , TOMMASINI, Roberto
IPC分类号： B01L3/00
CPC分类号： B01L3/502 , B01J2219/00533 , B01J2219/00659 , B01J2219/00722 , B01L3/502715 , B01L7/52 , B01L2200/0689 , B01L2300/0636 , B01L2300/0819 , B01L2300/0822 , B01L2300/0877 , B01L2400/0487 , C40B40/06 , G01N1/312 , G02B21/34
摘要： A reaction chamber assembly comprising a e.g. microscope slide or any other slide or carrier system and an assembly cover is described, wherein said assembly cover comprises at least one port and at least one channel having a first end at the port and a second end at a reaction compartment which reaction compartment together with the e.g. microscope slide forms a reaction chamber with predetermined volume.
摘要翻译：一种反应室组件，包括：描述了显微镜载玻片或任何其它滑动件或载体系统和组件盖，其中所述组件盖包括至少一个端口和至少一个通道，该通道在端口处具有第一端，第二端包括反应室，反应室与例如显微镜载玻片形成具有预定体积的反应室。

70. 发明申请

WO2003102234A1 METHOD FOR HIGH-THROUGHPUT INTEGRATED CHEMICAL AND BIOCHEMICAL REACTIONS 审中-公开
标题翻译：高通量整合化学和生化反应的方法
公开(公告)号：WO2003102234A1
公开(公告)日：2003-12-11
申请号：PCT/EP2003/005750
申请日：2003-06-02
申请人： PAMGENE BV , VAN BEUNINGEN, Marinus, Gerardus, Johannus , BOENDER, Pieter, Jacob
发明人： VAN BEUNINGEN, Marinus, Gerardus, Johannus , BOENDER, Pieter, Jacob
IPC分类号： C12Q1/68
CPC分类号： G01N33/551 , B01J19/0046 , B01J2219/00286 , B01J2219/00317 , B01J2219/00351 , B01J2219/00353 , B01J2219/00416 , B01J2219/00421 , B01J2219/00533 , B01J2219/00576 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00628 , B01J2219/0063 , B01J2219/00635 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , C12Q1/6837 , C40B40/06 , C40B40/10 , C40B60/14 , Y10S436/805 , C12Q2565/629
摘要： The present invention relates to methods for monitoring in a high through-put fashion a multitude of molecular reaction processes, using a substrate, said substrate comprising a plurality of micro-channels, wherein each micro-channel has inlet and outlet open ends on opposing sides of said substrate, and wherein said micro-channels comprise at least one first reaction component, comprising the steps of:(a) contacting said micro-channels of said solid substrate with a sample, said contacting being via said inlet open ends, wherein said sample comprises an analyte, under conditions that allow said analyte to be specifically retained within said micro-channels by said first reaction component;(b) optionally removing excess of sample via said outlet open ends;(c) contacting the retained analyte with at least one second reaction component, said second reaction component being different from said first reaction component as defined in step (a), under conditions that allow a molecular reaction to take place, said reaction producing a signal;(d) detecting said signal and reading-out reaction results;(e) removing second reaction components via said outlet open ends;(f) repeating steps (c) to (e) at least once, wherein said at least one second reaction component as defined in (c) may be changed in composition; and,final detecting and reading-out of reaction results.The present invention also relates to the uses thereof as well as to microarrays and kits for performing said methods of the invention.
摘要翻译：本发明涉及以高通量方式监测多种分子反应过程的方法，使用基底，所述基底包括多个微通道，其中每个微通道在相对侧具有入口和出口开口端并且其中所述微通道包括至少一个第一反应组分，包括以下步骤：（a）使所述固体基质的所述微通道与样品接触，所述接触通过所述入口开口端，其中所述所述样品包含分析物，所述条件允许所述分析物通过所述第一反应组分特异性保留在所述微通道内;（b）任选地通过所述出口开口端去除过量的样品;（c）使所保留的分析物与至少一个第二反应组分，所述第二反应组分不同于步骤（a）中定义的所述第一反应组分，在允许分子反应的条件下所述反应产生信号;（d）检测所述信号和读出反应结果;（e）通过所述出口开口端去除第二反应组分;（f）重复步骤（c）至（e）至少一次，其中如（c）中定义的所述至少一种第二反应组分的组成可以改变; 以及反应结果的最终检测和读出。本发明还涉及其用途以及用于执行本发明方法的微阵列和试剂盒。

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