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    • 7. 发明申请
    • MICROFLUIDIC MIXING AND DISPENSING
    • 微流化混合和分配
    • WO2003016832A2
    • 2003-02-27
    • PCT/US2002/025653
    • 2002-08-13
    • BOSTON INNOVATION INC.KARG, Jeffrey, A.KRONCKE, Douglas, W.
    • KARG, Jeffrey, A.KRONCKE, Douglas, W.
    • G01F
    • B01L3/0265B01F5/0646B01F5/0652B01F11/0071B01F13/0059B01J19/0046B01J2219/00315B01J2219/0036B01J2219/00369B01L2200/0605B01L2300/0838B01L2400/0487B01L2400/0633B01L2400/065C40B60/14G01N1/38G01N35/10G01N35/1074G01N2035/1032
    • A micro fluidic dispensing tap (20), such as for use in screening assays, includes a 5 dispensing tube (11) translatable within a sealed reservoir housing (32), The tube has an outer surface and defines an inner cavity (25) open at a lower end of the tube, and a metering aperture (27) extending through a side wall of the tube between the inner cavity and the outer surface to define a known volume. The tube is movable against a seal (24) at a lower end of the reservoir housing between a first position, in which the metering aperture is disposed 10 below the seal, and a second position, in which the metering aperture is disposed above the seal and exposed to the reservoir (21) for entraining a discrete dose of a sample liquid (30) within the aperture. An injector (130) is configured to inject a known quantity ofa diluent (53) into the inner cavity of the tube and into fluidic contact with the dose of sample liquid in the aperture, such that the dose of sample liquid diffuses into the diluent to form a discrete 15 mixture for dispensing from an open end of the tube.
    • 例如用于筛选测定的微流体分配抽头(20)包括可在密封储存器壳体(32)内平移的5分配管(11)。该管具有外表面并且限定内部空腔(25)打开 以及在所述内腔和所述外表面之间延伸穿过所述管的侧壁的计量孔(27),以限定已知体积。 所述管可在所述储存器壳体的下端处抵靠所述密封件(24)的第一位置和第二位置之间移动,所述第一位置在所述第一位置和所述第二位置之间,所述计量孔设置在所述密封件的上方, 并暴露于储存器(21),用于夹带孔内的离散剂量的样品液体(30)。 注射器(130)被配置为将已知量的稀释剂(53)注射到管的内腔中并与孔中的样品液体的剂量流体接触,使得样品液体的剂量扩散到稀释剂中 形成离散的15混合物,用于从管的开口端分配。