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1. 发明申请

WO2019135091A1 METHOD FOR SELECTING POLYNUCLEOTIDES BASED ON ENZYME INTERACTION DURATION 审中-公开
公开(公告)号：WO2019135091A1
公开(公告)日：2019-07-11
申请号：PCT/GB2019/050029
申请日：2019-01-07
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： HERON, Andrew John , BOWEN, Rebecca Victoria
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , C12Q2521/513 , C12Q2525/191 , C12Q2525/204 , C12Q2527/113 , C12Q2565/631
摘要： A method for selecting polynucleotides, the method comprising: allowing a nucleic acid handling enzyme to move along multiple polynucleotides in a sample for a defined time period, wherein the enzyme is loaded onto each of the multiple polynucleotides and wherein one or more molecule of the enzyme moves along each of the multiple polynucleotides; and selecting polynucleotides based on whether or not the enzyme reaches the end of and/or unbinds from the polynucleotides in the defined time period.

2. 发明申请

WO2016055777A3 MODIFIED ENZYMES 审中-公开
标题翻译：改良酶
公开(公告)号：WO2016055777A3
公开(公告)日：2016-06-16
申请号：PCT/GB2015052916
申请日：2015-10-06
申请人： OXFORD NANOPORE TECH LTD
发明人： HERON ANDREW , BOWEN REBECCA VICTORIA , BRUCE MARK , JAYASINGHE LAKMAL , LLOYD JOSEPH HARGREAVES , SOEROES SZABOLCS , WALLACE ELIZABETH JAYNE , YOUD CHRISTOPHER PETER
IPC分类号： C07K14/35 , C12Q1/68
CPC分类号： C12Q1/6869 , C12Q1/68 , C12Q2521/513 , C12Q2565/631
摘要： The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
摘要翻译：本发明涉及可用于控制多核苷酸运动的修饰的Dda解旋酶，并且特别可用于测序多核苷酸。

3. 发明申请

WO2016059436A1 METHOD FOR NANOPORE RNA CHARACTERISATION 审中-公开
标题翻译：纳米RNA表征的方法
公开(公告)号：WO2016059436A1
公开(公告)日：2016-04-21
申请号：PCT/GB2015/053097
申请日：2015-10-19
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： GARALDE, Daniel Ryan , HERON, Andrew John , JAYASINGHE, Lakmal , TURNER, Daniel John , WHITE, James
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12N9/14 , C12Y306/04012 , G01N27/44791 , C12Q2521/513 , C12Q2525/121 , C12Q2525/197 , C12Q2565/631
摘要： The invention relates to a new method of characterising a target RNA polynucleotide by taking one or more measurements as the target RNA polynucleotide moves with respect to a transmembrane pore. The movement is controlled by a DNA helicase. The invention also relates to a modified RNA construct wherein the RNApolynucleotidehas been modified to increase DNA helicase binding thereto.
摘要翻译：本发明涉及通过在靶RNA多核苷酸相对于跨膜孔移动时进行一个或多个测量来表征靶RNA多核苷酸的新方法。运动由DNA解旋酶控制。本发明还涉及修饰的RNA构建体，其中已经修饰了RNA多核苷酸以增加与其结合的DNA解旋酶。

4. 发明申请

WO2016055777A2 MODIFIED ENZYMES 审中-公开
标题翻译：修饰酶
公开(公告)号：WO2016055777A2
公开(公告)日：2016-04-14
申请号：PCT/GB2015/052916
申请日：2015-10-06
申请人： OXFORD NANOPORE TECHNOLOGIES LIMITED
发明人： HERON, Andrew , BOWEN, Rebecca Victoria , BRUCE, Mark , JAYASINGHE, Lakmal , LLOYD, Joseph Hargreaves , SOEROES, Szabolcs , WALLACE, Elizabeth Jayne , YOUD, Christopher Peter
IPC分类号： C07K14/35
CPC分类号： C12N9/14 , C12Q1/68 , C12Q1/6869 , C12Y306/04012 , C12Q2521/513 , C12Q2565/631
摘要： The invention relates to modified Dda helicases which can be used to control the movement of polynucleotides and are particularly useful for sequencing polynucleotides.
摘要翻译：本发明涉及经修饰的Dda解旋酶，其可用于控制多核苷酸的移动，并且特别适用于测序多核苷酸。

5. 发明申请

WO2015026609A1 HELICASE DEPENDENT AMPLIFICATION OF DNA MOLECULES USING NUCLEOTIDE ANALOGS 审中-公开
标题翻译：使用核苷类似物对DNA分子的依赖性放大
公开(公告)号：WO2015026609A1
公开(公告)日：2015-02-26
申请号：PCT/US2014/050980
申请日：2014-08-14
申请人： BENNER, Steven , YANG, Zunyi
发明人： BENNER, Steven , YANG, Zunyi
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q1/6853 , C12Q2521/513 , C12Q2525/101 , C12Q2525/117 , C12Q2521/101
摘要： This invention covers processes for the isothermal amplification of DNA molecules having a preselected sequence. It is based on the unexpected discovery that primers having, at some positions, adenine substituted by 2-aminopurine or diaminopurine, guanine by inosine, thymine by 2-thiothymine, and cytosine by N4-ethylcytosine ("SAMRS nucleotides") were accepted by enzymes used in the standard helicase-dependent amplification (HDA). Further unexpected was the discovery that target nucleotides are efficiently amplified in an HDA-like process (hereinafter abbreviated as simply HDA) using substituted primers. Also discovered was the diminution of spurious products through the use of SAMRS-substituted primers.
摘要翻译：本发明涉及具有预选序列的DNA分子的等温扩增方法。基于意想不到的发现，在某些位置，由2-氨基嘌呤或二氨基嘌呤取代的腺嘌呤，由肌苷引起的鸟嘌呤，2-硫代胸腺嘧啶的胸腺嘧啶和N4-乙基胞嘧啶的胞嘧啶（“SAMRS核苷酸”）的引物被酶用于标准解旋酶依赖性扩增（HDA）。进一步意外的是，使用取代的引物，以HDA样方法（以下简称为HDA）有效扩增靶核苷酸。还发现通过使用SAMRS取代的引物减少杂质产物。

6. 发明申请

WO2010147804A1 METHOD FOR DNA DOUBLE-STRAND BREAK REPAIR IN VITRO AND APPLICATIONS THEREOF 审中-公开
标题翻译：用于DNA双重破裂修复的方法及其应用
公开(公告)号：WO2010147804A1
公开(公告)日：2010-12-23
申请号：PCT/US2010037793
申请日：2010-06-08
申请人： WISCONSIN ALUMNI RES FOUND , COX MICHAEL M , BATTISTA JOHN R , KLINGELE AUDREY J , PIECHURA JOSEPH R
发明人： COX MICHAEL M , BATTISTA JOHN R , KLINGELE AUDREY J , PIECHURA JOSEPH R
IPC分类号： C12N15/10
CPC分类号： C12Q1/6806 , C12Q1/6844 , C12Q2531/119 , C12Q2522/101 , C12Q2521/513
摘要： A method for DNA double-strand break repair in vitro is disclosed. The method comprises using RecA, the single-stranded DNA binding protein (SSB) and DNA polymerase, in addition to a single-stranded DNA targeting fragment.
摘要翻译：公开了一种体外DNA双链断裂修复的方法。该方法除了单链DNA靶向片段外还使用RecA，单链DNA结合蛋白（SSB）和DNA聚合酶。

7. 发明申请

WO2010088273A1 THERMOPHILIC HELICASE DEPENDENT AMPLIFICATION TECHNOLOGY WITH ENDPOINT HOMOGENOUS FLUORESCENT DETECTION 审中-公开
标题翻译：具有端点均质荧光检测的热致命性相关放大技术
公开(公告)号：WO2010088273A1
公开(公告)日：2010-08-05
申请号：PCT/US2010/022233
申请日：2010-01-27
申请人： QIAGEN GAITHERSBURG , DOSEEVA, Victoria , FORBES, Thomas , WOLFF, John , NAZARENKO, Irina
发明人： DOSEEVA, Victoria , FORBES, Thomas , WOLFF, John , NAZARENKO, Irina
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6844 , C12Q1/6865 , C12Q2563/131 , C12Q2527/137 , C12Q2521/513
摘要： Disclosed herein are methods of amplifying a target nucleic acid in a helicase- dependent reaction. Also disclosed are methods of amplifying and detecting a target nucleic acid in a helicase-dependent reaction as well as modified detection labels to assist in the detection.
摘要翻译：本文公开的是在解旋酶依赖性反应中扩增靶核酸的方法。还公开了在解旋酶依赖性反应中扩增和检测靶核酸的方法以及用于辅助检测的经修改的检测标记。

8. 发明申请

WO2007120808A3 A METHOD OF ASYMMETRIC HELICASE-DEPENDENT AMPLIFICATION FOR PROBE-BASED DETECTION OF TARGETS 审中-公开
标题翻译：用于基于探针检测目标的不对称依赖性依赖放大的方法
公开(公告)号：WO2007120808A3
公开(公告)日：2008-01-31
申请号：PCT/US2007009099
申请日：2007-04-14
申请人： BIOHELIX CORP , TANG WEN , KONG HUIMIN
发明人： TANG WEN , KONG HUIMIN
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q2561/101 , C12Q2531/107 , C12Q2521/513
摘要： A method Is provided for performing asymmetric helicase-dependent (HDA) amplification and detecting pathogens using asymmetric HDA and nucleic acid probes.
摘要翻译：提供了一种使用不对称HDA和核酸探针进行不对称解旋酶依赖（HDA）扩增和检测病原体的方法。

9. 发明申请

WO2003008624A2 NUCLEIC ACID AMPLIFICATION USING NICKING AGENTS 审中-公开
标题翻译：使用镍原子的核酸放大
公开(公告)号：WO2003008624A2
公开(公告)日：2003-01-30
申请号：PCT/US2002/022748
申请日：2002-07-15
申请人： KECK GRADUATE INSTITUTE , VAN NESS, Jeffrey , GALAS, David, J. , VAN NESS, Lori, K.
发明人： VAN NESS, Jeffrey , GALAS, David, J. , VAN NESS, Lori, K.
IPC分类号： C12Q
CPC分类号： C12Q1/6844 , B01J2219/00608 , B01J2219/0061 , B01J2219/00659 , B01J2219/00722 , C12Q2600/158 , C40B40/06 , C12Q2527/107 , C12Q2525/155 , C12Q2521/301 , C12Q2531/119 , C12Q2521/513 , C12Q2521/313 , C12Q2521/307 , C12Q2521/101
摘要： The present invention provides methods and kits for amplifying target nucleic acids (including whole genomes) using nicking agents. In certain aspects, the amplification does not require the use of any external oligonucleotide primers that are capable of annealing to a portion of the target nucleic acid. This invention is useful in many areas such as genetic disease diagnoses, forensic analyses and paleoarcheological studies.
摘要翻译：本发明提供使用切口剂扩增靶核酸（包括全基因组）的方法和试剂盒。在某些方面，扩增不需要使用能够退火到靶核酸的一部分的任何外部寡核苷酸引物。本发明在诸如遗传疾病诊断，法医分析和古生物学研究等许多领域中是有用的。

10. 发明申请

WO02044405A2 METHOD AND KIT FOR THE DIRECT IDENTIFICATION OF NUCLEOTIDE SEQUENCES, AMINO ACID SEQUENCES OR ANTIGENS 审中-公开
标题翻译：方法和试剂盒用于核苷酸，氨基酸序列或抗原的直接检测
公开(公告)号：WO02044405A2
公开(公告)日：2002-06-06
申请号：PCT/EP2001/013980
申请日：2001-11-30
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： C12Q1/6837 , C12Q2531/119 , C12Q2525/125 , C12Q2521/513
摘要： The invention relates to a method for the direct identification of target nucleotide sequences, target amino acid sequences or target antigens by the amplification of DNA using a DNA polymerase and a DNA double-strand destabilising enzyme. According to the invention, the target molecule is present in situ or in solution, or is directly immobilised, or immobilised by means of a capture probe on a solid phase. A DNA polymerase that is capable of strand displacement, dNTPs and a DNA double-strand destabilising enzyme are then added and the resultant amplification product is directly detected, the target being identified by means of said detection. To identify non-amplifiable target molecules, a coupling probe carrying a DNA matrix is first added and a DNA polymerase that is capable of strand displacement, dNTPs and a DNA double-strand destabilising enzyme are then added and the resultant amplification product is directly detected, the target being identified by means of said detection.
摘要翻译：本发明涉及一种用于直接检测靶核苷酸序列的方法，目标通过使用DNA聚合酶和DNA酶doppelstrangdestabilisierenden DNA的扩增，其中所述靶分子存在于原位或在溶液中或直接的氨基酸序列或靶抗原或具有链置换能力的DNA聚合酶，dNTPs和一个DNA doppelstrangdestabilisierendes酶的一组被固定在捕获探针到固相，加入，并直接检测所得到的扩增和目标被检测或nichtamplifizierbarer用于检测目标分子的最初检测到的于此随后具有链置换能力的DNA聚合酶，dNTPs和的DNA doppelstrangdestabilisierendes酶组成的组中相加，并直接检测所得到的扩增子和目标是通过检测单元检测出矩阵DNS发送耦合探针添加。

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