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1. 发明授权

US06830717B2 Cast membrane structures for sample preparation 有权
标题翻译：用于样品制备的铸膜结构
公开(公告)号：US06830717B2
公开(公告)日：2004-12-14
申请号：US10209390
申请日：2002-07-31
申请人： William Kopaciewicz , Donald G. Sheer , Todd E. Arnold , Vinay Goel
发明人： William Kopaciewicz , Donald G. Sheer , Todd E. Arnold , Vinay Goel
IPC分类号： B29C6500
CPC分类号： B01J20/28033 , B01D61/00 , B01D61/007 , B01D61/18 , B01D63/16 , B01D69/10 , B01D69/141 , B01J20/28004 , B01J20/28019 , B01J20/28026 , B01J20/28028 , B01J20/2803 , B01J2220/64 , B01L3/0275 , G01N30/466 , G01N30/6004 , G01N30/6052 , G01N30/6065 , G01N2030/528 , G01N2035/1053 , G01N2035/1055
摘要： A method for casting-in-place composite and/or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular housing size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a housing such as a pipette tip, thereby providing an effective platform for micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted, including selective bind/elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads' and volumes. The present invention also encompasses the composite structures as well as sample preparation devices containing the same. In a second preferred embodiment, self-retaining, self-supporting structures are cast in situ in a suitable housing and can be used for size-based separations wherein the cast structure acts as a semi-permeable barrier. The present invention also encompasses these structures as well as housings containing these structures.
摘要翻译：用于现浇复合和/或非填充结构的方法，其可用作吸附或反应介质或基于尺寸的分离。可以使用任何特定的壳体尺寸或构型，并且在保持膜三维结构的同时实现在聚合物中包含大量吸附颗粒。在第一优选实施方案中，复合结构包括捕获在多孔聚合物基底内的颗粒，并且被放置在诸如移液管尖端的壳体中，从而为微粒处理提供有效的平台。通过适当的颗粒化学选择，可以进行任何分离或纯化操作，包括选择性结合/洗脱色谱操作，样品质量负载小于1微克体积的几微升，以及较大的质量负载和体积。本发明还包括复合结构以及含有该结构的样品制备装置。在第二优选实施例中，自固位自支撑结构在合适的壳体中原位铸造，并且可用于基于尺寸的间隔，其中铸造结构用作半渗透屏障。本发明还包括这些结构以及包含这些结构的壳体。

2. 发明授权

US06998047B1 Cast membrane structures for sample preparation 有权
公开(公告)号：US06998047B1
公开(公告)日：2006-02-14
申请号：US09698378
申请日：2000-10-27
申请人： William Kopaciewicz , Donald G. Sheer , Todd E. Arnold , Vinay Goel
发明人： William Kopaciewicz , Donald G. Sheer , Todd E. Arnold , Vinay Goel
IPC分类号： B01D63/00
CPC分类号： B01L3/0275 , B01J20/28026 , B01J20/28033 , B01J2220/64 , B01L3/50255 , B01L2300/0681 , B01L2300/0829
摘要： A method for casting-in-place composite and/or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular housing size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a housing such as a pipette tip, thereby providing an effective platform for micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted, including selective bind/elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. The present invention also encompasses the composite structures as well as sample preparation devices containing the same. In a second preferred embodiment, self-retaining, self-supporting structures are cast in situ in a suitable housing and can be used for size-based separations wherein the cast structure acts as a semi-permeable barrier. The present invention also encompasses these structures as well as housings containing these structures.

3. 发明授权

US6048457A Cast membrane structures for sample preparation 失效
标题翻译：用于样品制备的铸膜结构
公开(公告)号：US6048457A
公开(公告)日：2000-04-11
申请号：US7320
申请日：1998-01-15
申请人： William Kopaciewicz , Donald G. Sheer , Todd E. Arnold , Vinay Goel
发明人： William Kopaciewicz , Donald G. Sheer , Todd E. Arnold , Vinay Goel
IPC分类号： G01N1/10 , B01D61/00 , B01D61/18 , B01D63/00 , B01D63/16 , B01D67/00 , B01D69/10 , B01D69/14 , B01J20/28 , B01L3/02 , C12M1/00 , C12M1/12 , C12M1/26 , G01N1/36 , G01N30/46 , G01N30/52 , G01N30/60 , G01N35/10
CPC分类号： B01J20/28033 , B01D61/00 , B01D61/007 , B01D61/18 , B01D63/16 , B01D69/10 , B01D69/141 , B01J20/28004 , B01J20/28019 , B01J20/28026 , B01J20/28028 , B01J20/2803 , B01L3/0275 , G01N30/6065 , B01J2220/64 , G01N2030/528 , G01N2035/1053 , G01N2035/1055 , G01N30/466 , G01N30/6004 , G01N30/6052
摘要： A method for casting-in-place composite and/or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular housing size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a housing such as a pipette tip, thereby providing an effective platform for micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted, including selective bind/elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. The present invention also encompasses the composite structures as well as sample preparation devices containing the same. In a second preferred embodiment, self-retaining, self-supporting structures are cast in situ in a suitable housing and can be used for size-based separations wherein the cast structure acts as a semi-permeable barrier. The present invention also encompasses these structures as well as housings containing these structures.
摘要翻译：用于现浇复合和/或非填充结构的方法，其可用作吸附或反应介质或基于尺寸的分离。可以使用任何特定的壳体尺寸或构型，并且在保持膜三维结构的同时实现在聚合物中包含大量的吸附颗粒。在第一优选实施方案中，复合结构包括捕获在多孔聚合物基底内的颗粒，并且被放置在诸如移液管尖端的壳体中，从而为微粒处理提供有效的平台。通过适当的颗粒化学选择，可以进行任何分离或纯化操作，包括选择性结合/洗脱色谱操作，样品质量负载小于1微克体积的几微升，以及较大的质量负载和体积。本发明还包括复合结构以及含有该结构的样品制备装置。在第二优选实施例中，自固位自支撑结构在合适的壳体中原位铸造，并且可用于基于尺寸的间隔，其中铸造结构用作半渗透屏障。本发明还包括这些结构以及包含这些结构的壳体。

4. 发明授权

US06200474B1 Cast membrane structures for sample prepartion 有权
标题翻译：铸造膜结构用于样品制备
公开(公告)号：US06200474B1
公开(公告)日：2001-03-13
申请号：US09366134
申请日：1999-08-02
申请人： William Kopaciewicz , Donald G. Sheer , Todd E. Arnold , Vinay Goel
发明人： William Kopaciewicz , Donald G. Sheer , Todd E. Arnold , Vinay Goel
IPC分类号： B01D6300
CPC分类号： B01J20/28033 , B01D61/00 , B01D61/007 , B01D61/18 , B01D63/16 , B01D69/10 , B01D69/141 , B01J20/28004 , B01J20/28019 , B01J20/28026 , B01J20/28028 , B01J20/2803 , B01J2220/64 , B01L3/0275 , G01N30/466 , G01N30/6004 , G01N30/6052 , G01N30/6065 , G01N2030/528 , G01N2035/1053 , G01N2035/1055
摘要： A method for casting-in-place composite and/or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular housing size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a housing such as a pipette tip, thereby providing an effective platform for micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted, including selective bind/elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. The present invention also encompasses the composite structures as well as sample preparation devices containing the same. In a second preferred embodiment, self-retaining, self-supporting structures are cast in situ in a suitable housing and can be used for size-based separations wherein the cast structure acts as a semi-permeable barrier. The present invention also encompasses these structures as well as housings containing these structures.
摘要翻译：用于现浇复合和/或非填充结构的方法，其可用作吸附或反应介质或基于尺寸的分离。可以使用任何特定的壳体尺寸或构型，并且在保持膜三维结构的同时实现在聚合物中包含大量吸附颗粒。在第一优选实施方案中，复合结构包括捕获在多孔聚合物基底内的颗粒，并且被放置在诸如移液管尖端的壳体中，从而为微粒处理提供有效的平台。通过适当的颗粒化学选择，可以进行任何分离或纯化操作，包括选择性结合/洗脱色谱操作，样品质量负载小于1微克体积的几微升，以及较大的质量负载和体积。本发明还包括复合结构以及含有该结构的样品制备装置。在第二优选实施例中，自固位自支撑结构在合适的壳体中原位铸造，并且可用于基于尺寸的间隔，其中铸造结构用作半渗透屏障。本发明还包括这些结构以及包含这些结构的壳体。

5. 发明授权

US06635201B1 Cast membrane structures for sample preparation 有权
标题翻译：用于样品制备的铸膜结构
公开(公告)号：US06635201B1
公开(公告)日：2003-10-21
申请号：US09291355
申请日：1999-04-14
申请人： William Kopaciewicz , Donald G. Sheer , Todd E. Arnold , Vinay Goel
发明人： William Kopaciewicz , Donald G. Sheer , Todd E. Arnold , Vinay Goel
IPC分类号： B29C6500
CPC分类号： B01J20/28033 , B01D61/00 , B01D61/007 , B01D61/18 , B01D63/16 , B01D69/10 , B01D69/141 , B01J20/28004 , B01J20/28019 , B01J20/28026 , B01J20/28028 , B01J20/2803 , B01J2220/64 , B01L3/0275 , G01N30/466 , G01N30/6004 , G01N30/6052 , G01N30/6065 , G01N2030/528 , G01N2035/1053 , G01N2035/1055
摘要： A method for casting-in-place composite and/or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular housing size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a housing such as a pipette tip, thereby providing an effective platform for micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted, including selective bind/elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. The present invention also encompasses the composite structures as well as sample preparation devices containing the same. In a second structures are cast in situ in a suitable housing and can be used for size-based separations wherein the cast structure acts as a semi-permeable barrier. The present invention also encompasses these structures as well as housings containing these structures.
摘要翻译：用于现浇复合和/或非填充结构的方法，其可用作吸附或反应介质或基于尺寸的分离。可以使用任何特定的壳体尺寸或构型，并且在保持膜三维结构的同时实现在聚合物中包含大量吸附颗粒。在第一优选实施方案中，复合结构包括捕获在多孔聚合物基底内的颗粒，并且被放置在诸如移液管尖端的壳体中，从而为微粒处理提供有效的平台。通过适当的颗粒化学选择，可以进行任何分离或纯化操作，包括选择性结合/洗脱色谱操作，样品质量负载小于1微克体积的几微升，以及较大的质量负载和体积。本发明还包括复合结构以及含有该结构的样品制备装置。在第二结构中，在合适的壳体中原位铸造，并且可以用于基于尺寸的间隔，其中铸造结构用作半渗透屏障。本发明还包括这些结构以及包含这些结构的壳体。

6. 发明授权

US5833860A Centrifugal adsorptive sample preparation device and method 失效
标题翻译：离心吸附样品制备装置及方法
公开(公告)号：US5833860A
公开(公告)日：1998-11-10
申请号：US623688
申请日：1996-03-29
申请人： William Kopaciewicz , Ingeborg Cann , Donald G. Sheer
发明人： William Kopaciewicz , Ingeborg Cann , Donald G. Sheer
IPC分类号： B01D61/00 , B01D61/18 , B01D63/06 , B01D63/16 , B01J20/28 , B01L3/00 , G01N1/34
CPC分类号： G01N1/405 , B01D61/00 , B01D61/18 , B01D63/061 , B01D63/16 , B01J20/28019 , B01J20/2803 , B01J20/28033 , B01J20/28052 , B01L3/502
摘要： Adsorptive sample preparation device and method effective for concentrating, desalting and/or purifying biomolecules. An adsorptive membrane is used to bind biomolecules, which biomolecules can then be eluted with a suitable desorption agent. In a preferred form, the adsorptive filter means is a particle-laden semipermeable membrane having at least one of its major surfaces, preferably both of its major surfaces, covered with a thin fabric. In the method of the invention for concentrating biomolecules or desalting or purify from a solution, the sample reservoir of the above-described adsorptive sample preparation device is filled with an analyte solution, placed in a fixed angle or swinging bucket centrifuge rotor, the solution is driven through the membrane, and analyte obtained as a result of adsorption on the membrane is recovered by washing and then eluting with a suitable desorption agent. A method of sealing the membrane in the device is also set forth.
摘要翻译：吸附样品制备装置和有效浓缩，脱盐和/或净化生物分子的方法。吸附膜用于结合生物分子，然后用合适的解吸剂洗脱生物分子。在优选形式中，吸附过滤装置是含有颗粒的半透膜，其具有其主要表面中的至少一个，优选其两个主表面，均被薄织物覆盖。在本发明的用于浓缩生物分子或从溶液中脱盐或纯化的方法中，上述吸附样品制备装置的样品储存器填充有分析物溶液，放置在固定角度或摆动式离心机转子中，溶液为通过膜驱动，通过洗涤回收作为吸附在膜上的结果的分析物，然后用合适的解吸剂洗脱。还阐述了密封装置中的膜的方法。

7. 发明授权

US5124041A Biomolecule sample immobilization 失效
标题翻译：生物分子样品固定
公开(公告)号：US5124041A
公开(公告)日：1992-06-23
申请号：US697916
申请日：1991-05-01
申请人： Donald G. Sheer , Michael L. Kochersperger
发明人： Donald G. Sheer , Michael L. Kochersperger
IPC分类号： B01D15/00 , B01D61/00 , B01D61/18 , B01L3/00 , C07K1/36
CPC分类号： B01L3/502 , B01D15/00 , B01D61/00 , B01D61/18 , C07K1/36 , B01D15/3819 , Y10T436/255
摘要： A filter system for separating and binding biomolecules contained in a solution includes a first filter means for selectively passing components of the solution that are smaller than the biomolecules, thereby concentrating the biomolecules in the solution. Also included is a binding membrane means having an affinity for the biomolecules for binding the biomolecules to the binding membrane means, with the binding membrane means being located where the biomolecules are concentrated by the first filter means. In the preferred mode, the binding membrane means is chosen to selectively bind the biomolecule of interest. According to a preferred method of the invention, biomolecules from a sample volume are bound to a binding membrane by urging the sample volume against a biomolecule-binding support membrane that is in contact with a cut-off filter membrane such that the sample volume will pass through the biomolecule-binding support membrane before passing through the cut-off filter membrane. The concept is to concentrate the biomolecules of interest by means of the cut-off membrane in the vicinity of the binding membrane, thereby increasing the binding yield on the binding membrane. In the preferred mode, the sample volume is urged against the binding membrane by centrifugation.
摘要翻译：用于分离和结合包含在溶液中的生物分子的过滤系统包括用于选择性地通过小于生物分子的溶液成分的第一过滤器装置，从而将生物分子浓缩在溶液中。还包括对生物分子具有亲和力以将生物分子结合到结合膜装置的结合膜装置，其中结合膜装置位于生物分子被第一过滤装置浓缩的位置。在优选的模式中，选择结合膜装置选择性地结合感兴趣的生物分子。根据本发明的优选方法，将来自样品体积的生物分子通过将样品体积抵抗与截止滤膜接触的生物分子结合支持膜而结合到结合膜，使得样品体积将通过通过生物分子结合支持膜，然后通过截止滤膜。该概念是通过截止膜在结合膜附近浓缩感兴趣的生物分子，从而增加结合膜上的结合产率。在优选模式中，通过离心将样品体积压向结合膜。

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