专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06998047B1 Cast membrane structures for sample preparation 有权
公开(公告)号：US06998047B1
公开(公告)日：2006-02-14
申请号：US09698378
申请日：2000-10-27
申请人： William Kopaciewicz , Donald G. Sheer , Todd E. Arnold , Vinay Goel
发明人： William Kopaciewicz , Donald G. Sheer , Todd E. Arnold , Vinay Goel
IPC分类号： B01D63/00
CPC分类号： B01L3/0275 , B01J20/28026 , B01J20/28033 , B01J2220/64 , B01L3/50255 , B01L2300/0681 , B01L2300/0829
摘要： A method for casting-in-place composite and/or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular housing size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a housing such as a pipette tip, thereby providing an effective platform for micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted, including selective bind/elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. The present invention also encompasses the composite structures as well as sample preparation devices containing the same. In a second preferred embodiment, self-retaining, self-supporting structures are cast in situ in a suitable housing and can be used for size-based separations wherein the cast structure acts as a semi-permeable barrier. The present invention also encompasses these structures as well as housings containing these structures.

2. 发明授权

US6048457A Cast membrane structures for sample preparation 失效
标题翻译：用于样品制备的铸膜结构
公开(公告)号：US6048457A
公开(公告)日：2000-04-11
申请号：US7320
申请日：1998-01-15
申请人： William Kopaciewicz , Donald G. Sheer , Todd E. Arnold , Vinay Goel
发明人： William Kopaciewicz , Donald G. Sheer , Todd E. Arnold , Vinay Goel
IPC分类号： G01N1/10 , B01D61/00 , B01D61/18 , B01D63/00 , B01D63/16 , B01D67/00 , B01D69/10 , B01D69/14 , B01J20/28 , B01L3/02 , C12M1/00 , C12M1/12 , C12M1/26 , G01N1/36 , G01N30/46 , G01N30/52 , G01N30/60 , G01N35/10
CPC分类号： B01J20/28033 , B01D61/00 , B01D61/007 , B01D61/18 , B01D63/16 , B01D69/10 , B01D69/141 , B01J20/28004 , B01J20/28019 , B01J20/28026 , B01J20/28028 , B01J20/2803 , B01L3/0275 , G01N30/6065 , B01J2220/64 , G01N2030/528 , G01N2035/1053 , G01N2035/1055 , G01N30/466 , G01N30/6004 , G01N30/6052
摘要： A method for casting-in-place composite and/or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular housing size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a housing such as a pipette tip, thereby providing an effective platform for micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted, including selective bind/elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. The present invention also encompasses the composite structures as well as sample preparation devices containing the same. In a second preferred embodiment, self-retaining, self-supporting structures are cast in situ in a suitable housing and can be used for size-based separations wherein the cast structure acts as a semi-permeable barrier. The present invention also encompasses these structures as well as housings containing these structures.
摘要翻译：用于现浇复合和/或非填充结构的方法，其可用作吸附或反应介质或基于尺寸的分离。可以使用任何特定的壳体尺寸或构型，并且在保持膜三维结构的同时实现在聚合物中包含大量的吸附颗粒。在第一优选实施方案中，复合结构包括捕获在多孔聚合物基底内的颗粒，并且被放置在诸如移液管尖端的壳体中，从而为微粒处理提供有效的平台。通过适当的颗粒化学选择，可以进行任何分离或纯化操作，包括选择性结合/洗脱色谱操作，样品质量负载小于1微克体积的几微升，以及较大的质量负载和体积。本发明还包括复合结构以及含有该结构的样品制备装置。在第二优选实施例中，自固位自支撑结构在合适的壳体中原位铸造，并且可用于基于尺寸的间隔，其中铸造结构用作半渗透屏障。本发明还包括这些结构以及包含这些结构的壳体。

3. 发明授权

US07309458B2 Multi-sided immersion formation of composite structures and method 有权
标题翻译：复合结构和方法的多面浸入形成
公开(公告)号：US07309458B2
公开(公告)日：2007-12-18
申请号：US10689121
申请日：2003-10-20
申请人： William Kopaciewicz , Phillip Clark , David Brewster
发明人： William Kopaciewicz , Phillip Clark , David Brewster
IPC分类号： B29C65/00
CPC分类号： B01D67/0009 , B01D2323/24 , B01D2325/12 , B01L3/50255 , B01L2200/12 , B01L2300/0829 , B01L2300/0851 , B01L2400/049 , Y10T436/25375 , Y10T436/255
摘要： Sample preparation device and method for desalting and concentrating samples prior to further analysis such as by MALDI TOF and/or electro-spray ionization (ESI) mass spectrometry. The device in accordance with an embodiment of the present invention contains a three dimensional structure preferably comprising a plurality of sorptive particles entrapped in a porous polymer matrix so as to form a device capable of carrying out solid phase extraction. The device is manufactured by introducing casting solution containing polymer and optionally particles into a housing, and subsequently exposing the device to a quench bath for a time sufficient to allow for solvent exchange and precipitation to form the composite structure in the housing. The present invention is also directed towards a method of sample preparation using the device of the present invention.
摘要翻译：在进一步分析之前，例如通过MALDI TOF和/或电喷雾离子化（ESI）质谱法进行脱盐和浓缩样品的样品制备装置和方法。根据本发明的实施方案的装置包含三维结构，其优选包含多个捕获在多孔聚合物基质中的吸附颗粒，以形成能够进行固相萃取的装置。该装置通过将含有聚合物和任选的颗粒的浇铸溶液引入壳体而制造，并且随后将该装置暴露于淬火浴中足以允许溶剂交换和沉淀以在壳体中形成复合结构的时间。本发明还涉及使用本发明的装置的样品制备方法。

4. 发明申请

US20060040395A1 Anti-clogging device and method for in-gel digestion applications 审中-公开
公开(公告)号：US20060040395A1
公开(公告)日：2006-02-23
申请号：US11209410
申请日：2005-08-23
申请人： Phillip Clark , Chris Scott , Marc Emerick , William Kopaciewicz , Donald Rising
发明人： Phillip Clark , Chris Scott , Marc Emerick , William Kopaciewicz , Donald Rising
IPC分类号： G01N31/00
CPC分类号： B01L3/50255 , B01J20/28026 , B01J20/28033 , B01L2200/0631 , B01L2300/0681 , B01L2300/0829 , B01L2400/049 , G01N1/40 , G01N1/405 , G01N27/44717 , Y10T436/10 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
摘要： An integrated proteomics sample preparation device and method for in-gel digestion of proteins and for desalting and concentrating samples prior to further analysis such as by MALDI TOF and/or electro-spray ionization (ESI) mass spectrometry. The device and method of the present invention allow for digestion, desalting and concentration of sample prior to analysis. More specifically, the device in accordance with an embodiment of the present invention includes a plurality of wells in fluid communication with a an outlet or drainage opening containing a three dimensional structure comprising a plurality of sorptive particles entrapped in a porous polymer matrix so as to form a device capable of carrying out solid phase extraction. In a preferred embodiment, the wells are configured so as to prevent a sample carrier present in the wells from clogging the outlet when subjected to a driving force such as vacuum. The device also reduces or eliminates overflowing of a well in the event a drain becomes clogged during automated operation.

5. 发明授权

US06869572B1 High density cast-in-place sample preparation card 有权
标题翻译：高密度现场样品制备卡
公开(公告)号：US06869572B1
公开(公告)日：2005-03-22
申请号：US09659241
申请日：2000-09-11
申请人： William Kopaciewicz , Cheryl Brucato
发明人： William Kopaciewicz , Cheryl Brucato
IPC分类号： B01J20/28 , B01L3/00 , G01N30/02 , B01D29/05
CPC分类号： B01L3/50255 , B01J20/28026 , B01L2200/141 , B01L2300/0829 , B01L2400/049 , G01N30/02 , Y10T436/25375 , Y10T436/255 , B01D15/3804
摘要： A card or insert having a plurality of recesses for a sample preparation device, the card containing cast-in-place composite and/or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular card size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a plurality of recesses in an insert for a multi-well sample preparation device, thereby providing an effective platform for high throughput micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted in multiplicity, including selective bind/elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. Manufacturing flexibility and high throughput is achieved. The card can be configured for direct analysis of bound sample without elution.
摘要翻译：具有用于样品制备装置的多个凹部的卡或插入件，该卡包含可用作吸附或反应性介质或基于尺寸的分离的现浇复合材料和/或非填充结构。可以使用任何特定的卡尺寸或构型，并且在保持膜三维结构的同时实现在聚合物中包含大量吸附颗粒。在第一优选实施方案中，复合结构包括捕获在多孔聚合物基质内的颗粒，并且在多孔样品制备装置的插入物中就地浇铸成多个凹槽，从而为高通量微孔提供了有效的平台处理。通过适当选择的颗粒化学，几乎任何分离或纯化操作都可以进行多重性，包括选择性结合/洗脱色谱操作，样品质量负载小于1微克体积的几微升，以及较大的质量负载，卷。实现了制造灵活性和高吞吐量。该卡可以配置为直接分析结合的样品而不洗脱。

6. 发明授权

US06635201B1 Cast membrane structures for sample preparation 有权
标题翻译：用于样品制备的铸膜结构
公开(公告)号：US06635201B1
公开(公告)日：2003-10-21
申请号：US09291355
申请日：1999-04-14
申请人： William Kopaciewicz , Donald G. Sheer , Todd E. Arnold , Vinay Goel
发明人： William Kopaciewicz , Donald G. Sheer , Todd E. Arnold , Vinay Goel
IPC分类号： B29C6500
CPC分类号： B01J20/28033 , B01D61/00 , B01D61/007 , B01D61/18 , B01D63/16 , B01D69/10 , B01D69/141 , B01J20/28004 , B01J20/28019 , B01J20/28026 , B01J20/28028 , B01J20/2803 , B01J2220/64 , B01L3/0275 , G01N30/466 , G01N30/6004 , G01N30/6052 , G01N30/6065 , G01N2030/528 , G01N2035/1053 , G01N2035/1055
摘要： A method for casting-in-place composite and/or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular housing size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a housing such as a pipette tip, thereby providing an effective platform for micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted, including selective bind/elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. The present invention also encompasses the composite structures as well as sample preparation devices containing the same. In a second structures are cast in situ in a suitable housing and can be used for size-based separations wherein the cast structure acts as a semi-permeable barrier. The present invention also encompasses these structures as well as housings containing these structures.
摘要翻译：用于现浇复合和/或非填充结构的方法，其可用作吸附或反应介质或基于尺寸的分离。可以使用任何特定的壳体尺寸或构型，并且在保持膜三维结构的同时实现在聚合物中包含大量吸附颗粒。在第一优选实施方案中，复合结构包括捕获在多孔聚合物基底内的颗粒，并且被放置在诸如移液管尖端的壳体中，从而为微粒处理提供有效的平台。通过适当的颗粒化学选择，可以进行任何分离或纯化操作，包括选择性结合/洗脱色谱操作，样品质量负载小于1微克体积的几微升，以及较大的质量负载和体积。本发明还包括复合结构以及含有该结构的样品制备装置。在第二结构中，在合适的壳体中原位铸造，并且可以用于基于尺寸的间隔，其中铸造结构用作半渗透屏障。本发明还包括这些结构以及包含这些结构的壳体。

7. 发明授权

US08007745B2 Anti-clogging device and method for in-gel digestion applications 有权
标题翻译：抗堵塞装置和凝胶消解应用的方法
公开(公告)号：US08007745B2
公开(公告)日：2011-08-30
申请号：US10154550
申请日：2002-05-24
申请人： Phillip Clark , Chris A. Scott , Marc Emerick , William Kopaciewicz , Donald B. Rising
发明人： Phillip Clark , Chris A. Scott , Marc Emerick , William Kopaciewicz , Donald B. Rising
IPC分类号： B01L3/00
CPC分类号： B01L3/50255 , B01J20/28026 , B01J20/28033 , B01L2200/0631 , B01L2300/0681 , B01L2300/0829 , B01L2400/049 , G01N1/40 , G01N1/405 , G01N27/44717 , Y10T436/10 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
摘要： An integrated proteomics sample preparation device and method for in-gel digestion of proteins and for desalting and concentrating samples prior to further analysis such as by MALDI TOF and/or electro-spray ionization (ESI) mass spectrometry. The device and method of the present invention allow for digestion, desalting and concentration of sample prior to analysis. More specifically, the device in accordance with an embodiment of the present invention includes a plurality of wells in fluid communication with a an outlet or drainage opening containing a three dimensional structure comprising a plurality of sorptive particles entrapped in a porous polymer matrix so as to form a device capable of carrying out solid phase extraction. In a preferred embodiment, the wells are configured so as to prevent a sample carrier present in the wells from clogging the outlet when subjected to a driving force such as vacuum. The device also reduces or eliminates overflowing of a well in the event a drain becomes clogged during automated operation.
摘要翻译：蛋白质组学样品制备装置和方法，用于在进一步分析之前，例如通过MALDI TOF和/或电喷雾电离（ESI）质谱法进行蛋白质的凝胶内消化和用于脱盐和浓缩样品。本发明的装置和方法允许在分析之前对样品进行消化，脱盐和浓缩。更具体地说，根据本发明实施例的装置包括多个与出口或排水开口流体连通的孔，所述孔包含三维结构，所述三维结构包含多个捕获在多孔聚合物基体中的吸附颗粒，从而形成能够进行固相萃取的装置。在一个优选实施例中，孔构造成防止存在于井中的样品载体在经受诸如真空的驱动力时堵塞出口。在自动操作过程中排水管堵塞的情况下，该设备还可以减少或消除井的溢流。

8. 发明申请

US20050100482A1 Multi-sided immersion formation of composite structures and method 有权
标题翻译：复合结构和方法的多面浸入形成
公开(公告)号：US20050100482A1
公开(公告)日：2005-05-12
申请号：US11008062
申请日：2004-12-09
申请人： William Kopaciewicz , Phillip Clark , David Brewster
发明人： William Kopaciewicz , Phillip Clark , David Brewster
IPC分类号： B01D15/08 , B01L3/00 , G01N33/00
CPC分类号： B01D67/0009 , B01D2323/24 , B01D2325/12 , B01L3/50255 , B01L2200/12 , B01L2300/0829 , B01L2300/0851 , B01L2400/049 , Y10T436/25375 , Y10T436/255
摘要： Sample preparation device and method for desalting and concentrating samples prior to further analysis such as by MALDI TOF and/or electro-spray ionization (ESI) mass spectrometry. The device in accordance with an embodiment of the present invention contains a three dimensional structure preferably comprising a plurality of sorptive particles entrapped in a porous polymer matrix so as to form a device capable of carrying out solid phase extraction. The device is manufactured by introducing casting solution containing polymer and optionally particles into a housing, and subsequently exposing the device to a quench bath for a time sufficient to allow for solvent exchange and precipitation to form the composite structure in the housing. The present invention is also directed towards a method of sample preparation using the device of the present invention.
摘要翻译：在进一步分析之前，例如通过MALDI TOF和/或电喷雾离子化（ESI）质谱法进行脱盐和浓缩样品的样品制备装置和方法。根据本发明的实施方案的装置包含三维结构，其优选包含多个捕获在多孔聚合物基质中的吸附颗粒，以形成能够进行固相萃取的装置。该装置通过将含有聚合物和任选的颗粒的浇铸溶液引入壳体而制造，并且随后将该装置暴露于淬火浴中足以允许溶剂交换和沉淀以在壳体中形成复合结构的时间。本发明还涉及使用本发明的装置的样品制备方法。

9. 发明授权

US06200474B1 Cast membrane structures for sample prepartion 有权
标题翻译：铸造膜结构用于样品制备
公开(公告)号：US06200474B1
公开(公告)日：2001-03-13
申请号：US09366134
申请日：1999-08-02
申请人： William Kopaciewicz , Donald G. Sheer , Todd E. Arnold , Vinay Goel
发明人： William Kopaciewicz , Donald G. Sheer , Todd E. Arnold , Vinay Goel
IPC分类号： B01D6300
CPC分类号： B01J20/28033 , B01D61/00 , B01D61/007 , B01D61/18 , B01D63/16 , B01D69/10 , B01D69/141 , B01J20/28004 , B01J20/28019 , B01J20/28026 , B01J20/28028 , B01J20/2803 , B01J2220/64 , B01L3/0275 , G01N30/466 , G01N30/6004 , G01N30/6052 , G01N30/6065 , G01N2030/528 , G01N2035/1053 , G01N2035/1055
摘要： A method for casting-in-place composite and/or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular housing size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a housing such as a pipette tip, thereby providing an effective platform for micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted, including selective bind/elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. The present invention also encompasses the composite structures as well as sample preparation devices containing the same. In a second preferred embodiment, self-retaining, self-supporting structures are cast in situ in a suitable housing and can be used for size-based separations wherein the cast structure acts as a semi-permeable barrier. The present invention also encompasses these structures as well as housings containing these structures.
摘要翻译：用于现浇复合和/或非填充结构的方法，其可用作吸附或反应介质或基于尺寸的分离。可以使用任何特定的壳体尺寸或构型，并且在保持膜三维结构的同时实现在聚合物中包含大量吸附颗粒。在第一优选实施方案中，复合结构包括捕获在多孔聚合物基底内的颗粒，并且被放置在诸如移液管尖端的壳体中，从而为微粒处理提供有效的平台。通过适当的颗粒化学选择，可以进行任何分离或纯化操作，包括选择性结合/洗脱色谱操作，样品质量负载小于1微克体积的几微升，以及较大的质量负载和体积。本发明还包括复合结构以及含有该结构的样品制备装置。在第二优选实施例中，自固位自支撑结构在合适的壳体中原位铸造，并且可用于基于尺寸的间隔，其中铸造结构用作半渗透屏障。本发明还包括这些结构以及包含这些结构的壳体。

10. 发明授权

US4804686A Cation-exchange support materials and method 失效
标题翻译：阳离子交换支持材料和方法
公开(公告)号：US4804686A
公开(公告)日：1989-02-14
申请号：US862749
申请日：1986-05-13
申请人： Frederick F. Regnier , William Kopaciewicz
发明人： Frederick F. Regnier , William Kopaciewicz
IPC分类号： B01J39/08 , B01D15/04 , B01J20/281 , B01J39/16 , B01J39/26 , G01N30/88
CPC分类号： B01J39/26 , B01J39/165
摘要： Cation-exchange support materials and methods for producing such materials are disclosed. In making the support materials, first a thin layer of an adsorbate comprising amine groups is adsorbed to an inorganic support material such as silica, alumina or titania. The adsorbed coating is then optionally crosslinked by a crosslinking agent such as epoxy resin or alkyl bromide. At least one amine group of the adsorbed crosslinked coating is then reacted, preferably in the presence of a proton scavenger, with an amount of a hydrophilic reagent sufficient to generate at least one carboxyl group. Alternatively, amine groups of the uncrosslinked adsorbed coating are reacted, preferably in the presence of a proton scavenger, with an amount of a hydrophilic polyfunctional reagent sufficient to simultaneously crosslink said coating and generate carboxyl groups.
摘要翻译：公开了用于生产这种材料的阳离子交换支撑材料和方法。在制备载体材料时，首先将包含胺基团的吸附物的薄层吸附到无机载体材料如二氧化硅，氧化铝或二氧化钛上。吸附的涂层然后任选地通过交联剂例如环氧树脂或烷基溴交联。然后吸附的交联涂层的至少一个胺基优选在质子清除剂存在下与一定量的足以产生至少一个羧基的亲水试剂反应。或者，未交联的吸附涂层的胺基优选在质子清除剂的存在下与一定量的亲水性多官能试剂反应，足以使所述涂层同时交联并产生羧基。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式