专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20100075312A1 QPCR ANALYSIS APPARATUS 审中-公开
标题翻译： QPCR分析仪
公开(公告)号：US20100075312A1
公开(公告)日：2010-03-25
申请号：US12443139
申请日：2007-09-27
申请人： Mark Davies , John Daly , Tara Dalton
发明人： Mark Davies , John Daly , Tara Dalton
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： B01L7/525 , B01L3/508 , B01L7/54 , B01L2300/0654 , B01L2300/0809 , B01L2300/1827 , B01L2400/0442 , B01L2400/0445 , G01N21/6428 , G01N2021/6439 , G01N2201/062
摘要： An apparatus (1) is for DNA amplification with quantitative measurements. A biological sample is held in a cell (2) for the amplification, the cell (2) defining a single space within which the sample rotates. On one side a copper heater (3) is located to supply heat to the cell (2), and on the other side there is a cooling copper block (4) withdrawing heat from the cell. The locations of the heater (3) and the cooling block (4) generate a natural convection loop internally within the cell (2) without need for active cooling—the block (4) passively cooling by withdrawing heat from the direction of the heater (3). A detector (9, 27) captures readings in real time and a processor (10) generates an S-curve for change of sample emission with time. The S-curve (FIGS. 4 and 5) also includes a thermal cycle number corresponding to the time parameter, so that the S-curve is given in the traditional qPCR intensity vs. cycle number.
摘要翻译：装置（1）用于通过定量测量进行DNA扩增。将生物样品保持在用于扩增的细胞（2）中，细胞（2）限定样品旋转的单个空间。在一侧，铜加热器（3）被定位成向电池（2）供应热量，另一侧有一个冷却铜块（4）从电池中取出热量。加热器（3）和冷却块（4）的位置在电池（2）内部产生自然对流回路，而不需要主动冷却 - 通过从加热器的方向抽出热量来阻止块（4）的被动冷却（ 3）。检测器（9,27）实时捕获读数，并且处理器（10）产生用于随时间改变样品发射的S曲线。 S曲线（图4和图5）还包括对应于时间参数的热循环数，使得S曲线以传统的qPCR强度对周期数给出。

2. 发明授权

US07993911B2 Microfluidic droplet queuing network 有权
标题翻译：微流控液滴排队网络
公开(公告)号：US07993911B2
公开(公告)日：2011-08-09
申请号：US12097455
申请日：2007-02-07
申请人： Mark Davies , Tara Dalton , Julie Garvey , Kieran Curran , Damian Curtin
发明人： Mark Davies , Tara Dalton , Julie Garvey , Kieran Curran , Damian Curtin
IPC分类号： C12M1/34
CPC分类号： B01J19/0046 , B01F13/0071 , B01F15/0201 , B01F15/0232 , B01J2219/00313 , B01J2219/00353 , B01J2219/00479 , B01J2219/00587 , B01J2219/00608 , B01L3/5025 , B01L3/502784 , B01L2200/0673 , B01L2300/0829 , B01L2400/0487 , Y10T436/25
摘要： A multi-port liquid bridge (1) adds aqueous phase droplets (10) in an enveloping oil phase carrier liquid (11) to a draft channel (4, 6). A chamber (3) links four ports, and it is permanently full of oil (11) when in use. Oil phase is fed in a draft flow from an inlet port (4) and exits through a draft exit port (6) and a compensating flow port (7). The oil carrier and the sample droplets (3) (“aqueous phase”) flow through the inlet port (5) with an equivalent fluid flow subtracted through the compensating port (7). The ports of the bridge (1) are formed by the ends of capillaries held in position in plastics housings. The phases are density matched to create an environment where gravitational forces are negligible. This results in droplets (10) adopting spherical forms when suspended from capillary tube tips. Furthermore, the equality of mass flow is equal to the equality of volume flow. The phase of the inlet flow (from the droplet inlet port (5) and the draft inlet port (4) is used to determine the outlet port (6) flow phase.
摘要翻译：多端口液体桥（1）将包含油相载体液体（11）中的水相液滴（10）加入到通风道（4,6）。室（3）连接四个端口，在使用时永久充满油（11）。油相在进气口（4）的进料流中进料，并通过排气口（6）和补偿流通口（7）排出。油载体和样品液滴（“水相”）以相当的流体流通过入口端口（5）流过补偿口（7）。桥梁（1）的端口由保持在塑料壳体中的位置的毛细管的端部形成。这些阶段是密度匹配的，以创造引力几乎可忽略的环境。这导致当从毛细管尖端悬浮时，液滴（10）采用球形。此外，质量流量的相等性等于体积流量的相等。入口流（从液滴入口端口5和进气口4）的相位用于确定出口（6）的流动相位。

3. 发明申请

US20100304443A1 SAMPLING DEVICE 失效
标题翻译：采样设备
公开(公告)号：US20100304443A1
公开(公告)日：2010-12-02
申请号：US12732494
申请日：2010-03-26
申请人： Mark Davies , Tara Dalton , Michael Sayers , Brian Chawke
发明人： Mark Davies , Tara Dalton , Michael Sayers , Brian Chawke
IPC分类号： C12P19/34 , C12M1/26
CPC分类号： B01L3/021 , B01L3/0293 , B01L7/52 , B01L2200/0642 , B01L2200/0673 , Y10T436/25 , Y10T436/25625
摘要： The present invention generally relates to devices, systems, and methods for acquiring and/or dispensing a sample without introducing a gas into a microfluidic system, such as a liquid bridge system. An exemplary embodiment provides a sampling device including an outer sheath; a plurality of tubes within the sheath, in which at least one of the tubes acquires a sample, and at least one of the tubes expels a fluid that is immiscible with the sample, in which the at least one tube that acquires the sample is extendable beyond a distal end of the sheath and retractable to within the sheath; and a valve connected to a distal portion of the sheath, in which the valve opens when the tube extends beyond the distal end and closes when the tube retracts to within the sheath.
摘要翻译：本发明一般涉及用于获取和/或分配样品而不将气体引入诸如液体桥系统的微流体系统中的装置，系统和方法。示例性实施例提供了一种包括外护套的取样装置; 鞘内的多个管，其中至少一个管获取样品，并且至少一个管排出与样品不混溶的流体，其中获取样品的至少一个管可扩展超出护套的远端并伸缩到护套内; 以及连接到所述护套的远端部分的阀，当所述管延伸超过所述远端时所述阀打开，并且当所述管缩回到所述护套内时所述阀闭合。

4. 发明申请

US20080277494A1 Liquid Bridge and System 有权
标题翻译：液桥和系统
公开(公告)号：US20080277494A1
公开(公告)日：2008-11-13
申请号：US12092261
申请日：2006-02-07
申请人： Mark Davies , Tara Dalton , Kieran Curran
发明人： Mark Davies , Tara Dalton , Kieran Curran
IPC分类号： B29C45/20
CPC分类号： B01L3/502784 , B01F13/0071 , B01F15/0201 , B01F15/0232 , B01F2215/0037 , B01L3/5025 , B01L3/502715 , B01L3/502746 , B01L7/525 , B01L2200/0673 , B01L2300/0829 , B01L2300/0838 , B01L2400/0478 , B01L2400/0487 , B01L2400/0633 , B01L2400/082 , C12Q1/686 , F17D1/08 , Y10T137/87676 , Y10T436/117497 , Y10T436/118339 , Y10T436/2575
摘要： A bridge (30) comprises a first inlet port (31) at the end of a capillary, a narrower second inlet port (32) which is an end of a capillary, an outlet port (33) which is an end of a capillary, and a chamber (34) for silicone oil. The oil is density-matched with the reactor droplets such that a neutrally buoyant environment is created within the chamber (34). The oil within the chamber is continuously replenished by the oil separating the reactor droplets. This causes the droplets to assume a stable capillary-suspended spherical form upon entering the chamber (34). The spherical shape grows until large enough to span the gap between the ports, forming an axisymmetric liquid bridge. The introduction of a second droplet from the second inlet port (32) causes the formation of an unstable funicular bridge that quickly ruptures from the, finer, second inlet port (32), and the droplets combine at the liquid bridge (30). In another embodiment, a droplet (55) segments into smaller droplets which bridge the gap between the inlet and outlet ports.
摘要翻译：桥（30）包括在毛细管端部的第一入口（31），作为毛细管端部的较窄的第二入口（32），作为毛细管的端部的出口（33）和用于硅油的室（34）。油与反应器液滴密度匹配，使得在室（34）内产生中性浮力的环境。室内的油通过分离反应器液滴的油不断补充。这使得液滴在进入室（34）时呈现稳定的毛细管悬浮球形。球形增长直到足够大以跨越端口之间的间隙，形成轴对称液桥。从第二入口端口（32）引入第二液滴导致形成不稳定的索道桥，其从较细的第二入口（32）快速破裂，并且液滴在液体桥（30）处结合。在另一个实施例中，液滴（55）分段成较小的液滴，桥接入口和出口之间的间隙。

5. 发明授权

US09631230B2 Methods and devices for digital PCR 有权
公开(公告)号：US09631230B2
公开(公告)日：2017-04-25
申请号：US12539343
申请日：2009-08-11
申请人： Mark Davies , Tara Dalton
发明人： Mark Davies , Tara Dalton
IPC分类号： C12Q1/68 , B01L3/00 , B01L7/00
CPC分类号： C12Q1/6851 , B01L3/502784 , B01L7/525 , B01L2300/0838 , B01L2300/0867 , B01L2400/0487 , C12Q2561/101 , C12Q2563/159 , C12Q2565/629
摘要： The invention provides methods of conducting a nucleic acid reaction, including methods for performing digital PCR using a “droplet-in-oil” technology. In the methods, the starting sampled is segmented at least partially into a set of sample droplets each containing on average about one or fewer copies of a target nucleic acid. The droplets are passed in a continuous flow of immiscible carrier fluid through a channel that passes through a thermal cycler, whereby the target is amplified. In one implementation, the droplets are about 350 nl each and the number of positively amplified droplets is counted at the near-saturation point.

6. 发明授权

US08298833B2 Liquid bridge and system 有权
标题翻译：液桥和系统
公开(公告)号：US08298833B2
公开(公告)日：2012-10-30
申请号：US12092261
申请日：2007-02-07
申请人： Mark Davies , Tara Dalton , Kieran Curran
发明人： Mark Davies , Tara Dalton , Kieran Curran
IPC分类号： G01N1/10
CPC分类号： B01L3/502784 , B01F13/0071 , B01F15/0201 , B01F15/0232 , B01F2215/0037 , B01L3/5025 , B01L3/502715 , B01L3/502746 , B01L7/525 , B01L2200/0673 , B01L2300/0829 , B01L2300/0838 , B01L2400/0478 , B01L2400/0487 , B01L2400/0633 , B01L2400/082 , C12Q1/686 , F17D1/08 , Y10T137/87676 , Y10T436/117497 , Y10T436/118339 , Y10T436/2575
摘要： A bridge (30) comprises a first inlet port (31) at the end of a capillary, a narrower second inlet port (32) which is an end of a capillary, an outlet port (33) which is an end of a capillary, and a chamber (34) for silicone oil. The oil is density-matched with the reactor droplets such that a neutrally buoyant environment is created within the chamber (34). The oil within the chamber is continuously replenished by the oil separating the reactor droplets. This causes the droplets to assume a stable capillary-suspended spherical form upon entering the chamber (34). The spherical shape grows until large enough to span the gap between the ports, forming an axisymmetric liquid bridge. The introduction of a second droplet from the second inlet port (32) causes the formation of an unstable funicular bridge that quickly ruptures from the, finer, second inlet port (32), and the droplets combine at the liquid bridge (30). In another embodiment, a droplet (55) segments into smaller droplets which bridge the gap between the inlet and outlet ports.
摘要翻译：桥（30）包括在毛细管端部的第一入口（31），作为毛细管端部的较窄的第二入口（32），作为毛细管的端部的出口（33）和用于硅油的室（34）。油与反应器液滴密度匹配，使得在室（34）内产生中性浮力的环境。室内的油通过分离反应器液滴的油不断补充。这使得液滴在进入室（34）时呈现稳定的毛细管悬浮球形。球形增长直到足够大以跨越端口之间的间隙，形成轴对称液桥。从第二入口端口（32）引入第二液滴导致形成不稳定的索道桥，其从较细的第二入口（32）快速破裂，并且液滴在液体桥（30）处结合。在另一个实施例中，液滴（55）分段成较小的液滴，桥接入口和出口之间的间隙。

7. 发明申请

US20100092987A1 MICROFLUIDIC ANALYSIS SYSTEM 审中-公开
标题翻译：微流感分析系统
公开(公告)号：US20100092987A1
公开(公告)日：2010-04-15
申请号：US12617286
申请日：2009-11-12
申请人： Mark Davies , Tara Dalton
发明人： Mark Davies , Tara Dalton
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/686 , B01F5/0057 , B01F13/0059 , B01L3/50273 , B01L3/502776 , B01L3/502784 , B01L7/525 , B01L2200/0636 , B01L2200/0673 , B01L2200/141 , B01L2300/0816 , B01L2300/0861 , B01L2300/087 , B01L2300/185 , B01L2400/0409 , B01L2400/0487 , G01N35/08 , G01N35/1095 , G01N2035/00514
摘要： A microfluidic analysis system (1) performs polymerase chain reaction (PCR) analysis on a bio sample. In a centrifuge (6) the sample is separated into DNA and RNA constituents. The vortex is created by opposing flow of a silicon oil primary carrier fluid effecting circulation by viscous drag. The bio sample exits the centrifuge enveloped in the primary carrier fluid. This is pumped by a flow controller (7) to a thermal stage (9). The thermal stage (9) has a number of microfluidic devices (70) each having thermal zones (71, 72, 73) in which the bio sample is heated or cooled by heat conduction to/from a thermal carrier fluid and the primary carrier fluid. Thus, the carrier fluids envelope the sample, control its flowrate, and control its temperature without need for moving parts at the micro scale.
摘要翻译：微流体分析系统（1）对生物样品进行聚合酶链反应（PCR）分析。在离心机（6）中，将样品分离成DNA和RNA成分。涡流通过由粘性阻力影响循环的硅油主载流体的相反流动而产生。生物样品离开包围在主载体流体中的离心机。这被流量控制器（7）泵送到热级（9）。热级（9）具有多个微流体装置（70），每个微流体装置（70）各自具有热区（71,72,73），其中生物样品通过向热载体流体和主载体流体的热传导而被加热或冷却。因此，载体流体包裹样品，控制其流速，并控制其温度，而不需要微量级的移动部件。

8. 发明申请

US20100041055A1 NOVEL GENE NORMALIZATION METHODS 审中-公开
标题翻译：新基因正常化方法
公开(公告)号：US20100041055A1
公开(公告)日：2010-02-18
申请号：US12539773
申请日：2009-08-12
申请人： Mark Davies , Tara Dalton
发明人： Mark Davies , Tara Dalton
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/6809 , C12Q1/6886 , C12Q2545/101
摘要： Measurement of gene expression relative to an endogenous control gene is prone to excessive variability between samples and even replicates. The disclosure provides methods for normalizing expression levels of a gene by scaling gene expression levels to that of the most highly expressed gene in the set of genes whose expression levels are measured, rather than a house-keeping gene.
摘要翻译：相对于内源性对照基因的基因表达的测量在样品之间容易发生过度的变异，甚至复制。本公开提供了通过将基因表达水平与表达水平测定的基因组中最高表达基因的基因表达水平相比较而不是保存基因来使基因表达水平正常化的方法。

9. 发明授权

US09597644B2 Methods for culturing and analyzing cells 有权
公开(公告)号：US09597644B2
公开(公告)日：2017-03-21
申请号：US12732769
申请日：2010-03-26
申请人： Mark Davies , Tara Dalton
发明人： Mark Davies , Tara Dalton
IPC分类号： C12Q1/68 , B01F13/00 , B01F5/00 , B01L3/00 , B01L7/00 , G01N33/50 , G01N35/08 , G01N35/10 , G01N35/00
CPC分类号： B01F5/0057 , B01F13/0059 , B01F13/0071 , B01L3/502784 , B01L7/525 , B01L2200/0636 , B01L2200/0673 , B01L2300/0816 , B01L2300/0861 , B01L2300/087 , B01L2300/185 , B01L2400/0409 , B01L2400/0487 , G01N33/5008 , G01N35/08 , G01N35/1095 , G01N2035/00514
摘要： Provided herein is a method for analyzing the affect of at least one agent on a cell or cellular component using an analysis system. The method includes wrapping a droplet in an immiscible carrier fluid using a droplet generator wherein the wrapped droplet includes at least one cell or cellular component and at least one agent. The wrapped droplet then flows through the analysis system using a siphoning effect. The droplet is then analyzed to determine effect of the agent on the cell or cellular component.

10. 发明授权

US08741660B2 Sampling device 失效
标题翻译：取样装置
公开(公告)号：US08741660B2
公开(公告)日：2014-06-03
申请号：US12732494
申请日：2010-03-26
申请人： Mark Davies , Tara Dalton , Michael Sayers , Brian Chawke
发明人： Mark Davies , Tara Dalton , Michael Sayers , Brian Chawke
IPC分类号： G01N1/18
CPC分类号： B01L3/021 , B01L3/0293 , B01L7/52 , B01L2200/0642 , B01L2200/0673 , Y10T436/25 , Y10T436/25625
摘要： The present invention generally relates to devices, systems, and methods for acquiring and/or dispensing a sample without introducing a gas into a microfluidic system, such as a liquid bridge system. An exemplary embodiment provides a sampling device including an outer sheath; a plurality of tubes within the sheath, in which at least one of the tubes acquires a sample, and at least one of the tubes expels a fluid that is immiscible with the sample, in which the at least one tube that acquires the sample is extendable beyond a distal end of the sheath and retractable to within the sheath; and a valve connected to a distal portion of the sheath, in which the valve opens when the tube extends beyond the distal end and closes when the tube retracts to within the sheath.
摘要翻译：本发明一般涉及用于获取和/或分配样品而不将气体引入诸如液体桥系统的微流体系统中的装置，系统和方法。示例性实施例提供了一种包括外护套的取样装置; 鞘内的多个管，其中至少一个管获取样品，并且至少一个管排出与样品不混溶的流体，其中获取样品的至少一个管可扩展超出护套的远端并伸缩到护套内; 以及连接到所述护套的远端部分的阀，当所述管延伸超过所述远端时所述阀打开，并且当所述管缩回到所述护套内时所述阀闭合。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式