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1. 发明授权

US10864521B2 Detection mechanism for polymerase chain reaction and polymerase chain reaction device 有权
公开(公告)号：US10864521B2
公开(公告)日：2020-12-15
申请号：US15774953
申请日：2016-10-25
申请人： Beijing Wantai Biological Pharmacy Enterprise Co., Ltd. , Beijing University of Chemical Technology , Xiamen University
发明人： Xianbo Qiu , Xiangzhong Ye , Shengxiang Ge , Shiyin Zhang , Pengfei Gao , Shangzhi Ji , Yongliang Yang , Zixin Qiu , Ningshao Xia
IPC分类号： B01L7/00 , G01N21/64
CPC分类号： B01L7/52 , B01L7/525 , B01L7/54 , G01N21/645 , G01N21/6428 , G01N21/6452 , B01L2200/147 , B01L2300/1805 , B01L2400/0445 , G01N2021/6419 , G01N2021/6421 , G01N2021/6471 , G01N2021/6484 , G01N2201/0627 , G01N2201/08 , G01N2201/0826 , G01N2201/0833
摘要： The present invention relates to a detection mechanism for polymerase chain reaction and a polymerase chain reaction device, wherein the detection mechanism comprises at least one excitation module group, each of the excitation module groups comprising two excitation modules for providing excitation light with two wavelengths; an excitation optical fiber, connected to the excitation modules, the excitation optical fiber transmitting the excitation light to at least one reaction tube, each of the reaction tubes receiving excitation light with two wavelengths; a receiving optical fiber, for collecting and transmitting a fluorescent signal from the reaction tube; at least one receiving module group, connected to the receiving optical fiber, each of the receiving module groups comprising two receiving modules, to respectively receive the fluorescent signal of two wavelengths from the same said reaction tube, and convert the fluorescent signal into an electrical signal for output; the detection mechanism is configured to detect the reaction tube in a time division manner, and multiplex the receiving module group to obtain an output result.

2. 发明授权

US10487301B2 Reaction tube for nucleic acid amplification capable of controlling liquid circulation path 有权
公开(公告)号：US10487301B2
公开(公告)日：2019-11-26
申请号：US15573381
申请日：2016-05-11
申请人： XIAMEN UNIVERSITY , XIAMEN INNODX BIOTECH CO., LTD
发明人： Shengxiang Ge , Shiyin Zhang , Feihai Xu , Jin Wang , Jinjie Li , Jun Zhang , Ningshao Xia
IPC分类号： C12M1/38 , C12M1/24 , B01L3/00 , B01L7/00 , C12Q1/6844
CPC分类号： C12M1/38 , B01L3/5082 , B01L3/50273 , B01L3/502753 , B01L7/525 , B01L7/54 , C12M1/24 , C12Q1/6844 , B01L2300/047 , B01L2300/1805 , B01L2400/0445
摘要： Disclosed are a reaction tube for nucleic acid amplification capable of controlling a liquid circulation path, a reaction apparatus for nucleic acid amplification comprising the reaction tube, and a method for amplifying nucleic acid comprising a step of using the reaction tube. Also disclosed are a kit comprising the reaction tube, and use of the reaction tube in preparation of a kit.

3. 发明申请

US20180104696A1 CONVECTIVE POLYMERASE CHAIN REACTION APPARATUS AND OPTICAL DETECTING METHOD THEREOF 审中-公开
公开(公告)号：US20180104696A1
公开(公告)日：2018-04-19
申请号：US15588043
申请日：2017-05-05
申请人： INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE
发明人： Tseng-Huang Liu , Kuo-Hsing Wen , Pei-Shin Jiang , Jie-Len Huang , Ting-Hsuan CHEN
IPC分类号： B01L7/00 , C12Q1/68
CPC分类号： B01L7/52 , B01L7/525 , B01L2300/0654 , B01L2300/0681 , B01L2300/0832 , B01L2300/18 , B01L2400/0445 , C12Q1/686 , G01N21/00
摘要： A convective polymerase chain reaction apparatus includes a tube, a temperature control unit, at least one light source and a sensor. The tube includes a cavity used to contain a reaction solution. The reaction solution has a liquid level measured from a bottom of the cavity to a top surface of the reaction solution. The temperature control unit is disposed adjacent to the tube for controlling the temperature of the reaction solution. The at least one light source provides a light beam passing through an incident portion of the tube to excite the reaction solution emitting a fluorescent light. The incident portion is located at a height greater than ½ of a liquid level. The sensor is adjacent to the tube for detecting the excited fluorescence. The light beam has an incident direction forming a non-straight angle with a long axis of the tube.

4. 发明申请

US20160318023A1 CHANNELS WITH CROSS-SECTIONAL THERMAL GRADIENTS 审中-公开
公开(公告)号：US20160318023A1
公开(公告)日：2016-11-03
申请号：US14981158
申请日：2015-12-28
申请人： ABBOTT MOLECULAR INC.
发明人： Herbert A. Marble , Thomas Laffler
IPC分类号： B01L7/00 , B01L3/00 , C12P19/34 , C12Q1/68
CPC分类号： B01L7/54 , B01L3/502784 , B01L7/525 , B01L2400/0415 , B01L2400/0433 , B01L2400/0445 , B01L2400/086 , C07H21/00 , C12P19/34 , C12Q1/686 , C12Q2527/15 , C12Q2563/159 , C12Q2565/629
摘要： Provided herein are systems, devices, and methods for generating thermal gradients in channels and uses thereof. In particular, provided herein are system, methods, and devices employing first and second thermal layers positioned around a channel in order to create a thermal gradient across a cross-section of the channel having, for example, a nucleic acid denaturation zone, a nucleic acid annealing zone, and a nucleic acid polymerization zone. Such devices find use in, for example, nucleic acid amplification procedures, including digital polymerase chain reaction (dPCR) to temperature cycle droplets for amplification of nucleic acid templates within the droplets.

5. 发明申请

US20140154786A1 Chemical Temperature Control 审中-公开
标题翻译：化学温度控制
公开(公告)号：US20140154786A1
公开(公告)日：2014-06-05
申请号：US13874060
申请日：2013-04-30
申请人： Program For Appropriate Technology In Health
发明人： Paul Donald Labarre , Jay Lewis Gerlach , Bernhard Hans Weigl , Gonzalo Jose Domingo-Villegas
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/50273 , B01L7/00 , B01L7/525 , B01L2300/0663 , B01L2300/0816 , B01L2300/088 , B01L2300/1855 , B01L2300/1877 , B01L2400/0406 , B01L2400/0442 , B01L2400/0445 , B01L2400/0478 , B01L2400/0487
摘要： Exothermic and/or endothermic chemical reactions in combination with phase change materials can produce output temperature(s) within strict tolerances without requiring expensive and complicated external equipment to generate and maintain an output temperature. Similarly, an exothermic phase change material, which generates heat as a consequence of crystallizing a supercooled liquid, can generate heat at a constant temperature, without requiring expensive and complicated external equipment, as a consequence of the liquid form of the exothermic phase change material being in equilibrium with the solid form of the exothermic phase change material. Numerous biological and chemical processes and/or diagnostic devices require a constant temperature or temperatures for set periods of time. An example completely non-instrumented diagnostic platform based on nucleic acid amplification is described, which is particularly suited for use in developing countries that may not have access to expensive and complicated external equipment.
摘要翻译：与相变材料相结合的放热和/或吸热化学反应可以在严格的公差范围内产生输出温度，而不需要昂贵和复杂的外部设备来产生和保持输出温度。类似地，作为结晶过冷液体的结果产生热量的放热相变材料可以在恒定温度下产生热量，而不需要昂贵且复杂的外部设备，因为放热相变材料的液体形式是与固体形式的放热相变材料平衡。许多生物和化学过程和/或诊断装置需要恒定的温度或温度来设定一段时间。描述了基于核酸扩增的完全非仪器化诊断平台的示例，其特别适用于可能无法访问昂贵且复杂的外部设备的发展中国家。

6. 发明申请

US20130330818A1 THERMAL CYCLER 有权
标题翻译：热循环
公开(公告)号：US20130330818A1
公开(公告)日：2013-12-12
申请号：US13910462
申请日：2013-06-05
申请人： Seiko Epson Corporation
发明人： Hiroshi KOEDA , Toshiro MURAYAMA
IPC分类号： C12M1/34
CPC分类号： C12M41/12 , B01L7/525 , B01L7/5255 , B01L7/54 , B01L2300/1827 , B01L2400/0445 , B01L2400/0457 , B01L2400/0469
摘要： A thermal cycler includes: a first mounting unit and a second mounting unit which are cylindrical; a temperature gradient forming unit which forms a temperature gradient along mounting directions of the first mounting unit and the second mounting unit; and a driving mechanism which rotates the first mounting unit, the second mounting unit, and the temperature gradient forming unit around a rotating shaft having a component perpendicular to a direction in which gravity is applied and a component intersecting the mounting directions of the first mounting unit and the second mounting unit, wherein the first mounting unit and the second mounting unit are disposed on opposite sides to each other with the rotating shaft interposed therebetween, the mounting direction of the first mounting unit and the mounting direction of the second mounting unit are in the same direction.
摘要翻译：热循环仪包括：圆柱形的第一安装单元和第二安装单元; 温度梯度形成单元，其沿着第一安装单元和第二安装单元的安装方向形成温度梯度; 以及驱动机构，其使第一安装单元，第二安装单元和温度梯度形成单元围绕具有与重力施加的方向垂直的分量的旋转轴旋转，并且与第一安装单元的安装方向相交的部件和第二安装单元，其中第一安装单元和第二安装单元彼此相对设置，其中转轴插入其间，第一安装单元的安装方向和第二安装单元的安装方向在方向相同

7. 发明申请

US20130183717A1 CHANNELS WITH CROSS-SECTIONAL THERMAL GRADIENTS 有权
标题翻译：具有跨部分热梯度的通道
公开(公告)号：US20130183717A1
公开(公告)日：2013-07-18
申请号：US13724503
申请日：2012-12-21
申请人： Herbert A. Marble , Thomas Laffler
发明人： Herbert A. Marble , Thomas Laffler
IPC分类号： C12P19/34
CPC分类号： B01L7/54 , B01L3/502784 , B01L7/525 , B01L2400/0415 , B01L2400/0433 , B01L2400/0445 , B01L2400/086 , C07H21/00 , C12P19/34 , C12Q1/686 , C12Q2527/15 , C12Q2563/159 , C12Q2565/629
摘要： Provided herein are systems, devices, and methods for generating thermal gradients in channels and uses thereof. In particular, provided herein are system, methods, and devices employing first and second thermal layers positioned around a channel in order to create a thermal gradient across a cross-section of the channel having, for example, a nucleic acid denaturation zone, a nucleic acid annealing zone, and a nucleic acid polymerization zone. Such devices find use in, for example, nucleic acid amplification procedures, including digital polymerase chain reaction (dPCR) to temperature cycle droplets for amplification of nucleic acid templates within the droplets.
摘要翻译：本文提供了用于在通道中产生热梯度的系统，装置和方法及其用途。特别地，本文提供了使用位于通道周围的第一和第二热层的系统，方法和装置，以便跨越具有例如核酸变性区的通道的横截面产生热梯度，核酸酸退火区和核酸聚合区。这样的装置可用于例如核酸扩增程序，包括数字聚合酶链反应（dPCR）到温度循环液滴，用于扩散液滴内的核酸模板。

8. 发明申请

US20080131956A1 NATURAL CONVECTION-DRIVEN PCR APPARATUS AND METHOD USING DISPOSABLE POLYMER CHIP 有权
标题翻译：自然对流驱动PCR装置和使用可分散聚合物芯片的方法
公开(公告)号：US20080131956A1
公开(公告)日：2008-06-05
申请号：US11866979
申请日：2007-10-03
申请人： Kwang-Hyo CHUNG , Dae-Sik Lee , Hyeon-Bong Pyo , Seon-Hee Park
发明人： Kwang-Hyo CHUNG , Dae-Sik Lee , Hyeon-Bong Pyo , Seon-Hee Park
IPC分类号： C12M1/36
CPC分类号： C12Q1/686 , B01L3/5027 , B01L3/50273 , B01L7/52 , B01L7/525 , B01L2300/088 , B01L2300/1827 , B01L2400/0445 , B01L2400/0472 , B01L2400/0688 , B33Y80/00 , C12Q1/6844
摘要： Provided is a natural convection-driven polymerase chain reaction (PCR) apparatus and method, which can periodically change temperature of a sample to make a natural convection-driven flow in a loop-shaped channel on a disposable polymer chip and contacting heating metal members maintained at different temperatures with channels. The natural convection-driven PCR apparatus includes: a polymer chip having a plurality of channels connected together to form a loop-shaped microchannel, a sample being filled inside the channels; a plurality of heating metal members contacting the loop-shaped microchannel of the polymer chip and transferring heat; a temperature controller connected to the heating metal members to maintain the heating metal members at different temperatures; a plurality of heaters connected to the heating metal members and the temperature controller to supply heat to the heating metal members; and temperature sensors connected to the heating metal members and the temperature controller.
摘要翻译：提供了一种自然对流驱动的聚合酶链反应（PCR）装置和方法，其可以周期性地改变样品的温度以在一次性聚合物芯片上的环形通道中进行自然对流驱动的流动，并且接触保持的加热金属构件在不同的温度与通道。自然对流驱动PCR装置包括：聚合物芯片，具有连接在一起形成环形微通道的多个通道，样品填充在通道内; 与聚合物芯片的环形微通道接触并传递热量的多个加热金属构件; 连接到所述加热金属部件以将所述加热金属部件保持在不同温度的温度控制器; 连接到加热金属部件的多个加热器和温度控制器，以向加热金属部件供热; 温度传感器连接到加热金属部件和温度控制器。

9. 发明申请

US20030087292A1 Methods and systems for promoting interactions between probes and target molecules in fluid in microarrays 审中-公开
标题翻译：促进微阵列流体中探针与靶分子之间相互作用的方法和系统
公开(公告)号：US20030087292A1
公开(公告)日：2003-05-08
申请号：US10264892
申请日：2002-10-04
发明人： Shiping Chen , Jianming Xiao , Kaijun Li , Hoang M. Nguyen
IPC分类号： C12Q001/68 , C12M001/34
CPC分类号： B01J19/0046 , B01F5/061 , B01F5/0645 , B01F5/0647 , B01F9/0003 , B01F9/0014 , B01F11/0014 , B01F11/0042 , B01F11/0051 , B01F11/0071 , B01F11/0266 , B01F13/0059 , B01F13/0076 , B01F13/0081 , B01F13/0818 , B01F15/00883 , B01F2005/0631 , B01F2005/0636 , B01J2219/00313 , B01J2219/0036 , B01J2219/00421 , B01J2219/00466 , B01J2219/00479 , B01J2219/00495 , B01J2219/00533 , B01J2219/00585 , B01J2219/00596 , B01J2219/00659 , B01J2219/00662 , B01J2219/00722 , B01J2219/00743 , B01L3/50273 , B01L3/5088 , B01L2200/0673 , B01L2300/0636 , B01L2300/0819 , B01L2300/0822 , B01L2300/088 , B01L2300/0883 , B01L2300/089 , B01L2300/1822 , B01L2400/0415 , B01L2400/043 , B01L2400/0433 , B01L2400/0445 , B01L2400/0481 , B01L2400/0487 , B01L2400/084 , B01L2400/088 , C12Q1/6834 , C40B60/12
摘要： Methods and apparatus for promoting interactions between an array of probes deposited on a microarray substrate and target molecules in a target liquid are provided. A microarray apparatus can include a substrate having an array of probes deposited on a surface of the substrate for interaction with a target molecule in a target liquid. The apparatus also includes a cover coupled to the substrate to form a reaction chamber therebetween, wherein the array of probes is contained within the reaction chamber and the substrate and the cover are movable relative to each other.
摘要翻译：提供了用于促进沉积在微阵列基底上的探针阵列与目标液体中的靶分子之间的相互作用的方法和装置。微阵列装置可以包括具有沉积在基底表面上的探针阵列的基底，用于与目标液体中的靶分子相互作用。所述设备还包括联接到所述基板以在其间形成反应室的盖，其中所述探针阵列包含在所述反应室内，并且所述基板和所述盖可相对于彼此移动。

10. 发明公开

US20240183771A1 Method and apparatus for spatially manipulating at least one particle in a fluid, computer program product and computer-readable storage medium 审中-公开
公开(公告)号：US20240183771A1
公开(公告)日：2024-06-06
申请号：US18574608
申请日：2021-07-30
申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften E.V.
发明人： Elena Erben , Nicola Maghelli , Moritz Kreysing
IPC分类号： G01N15/1409 , B01L3/00 , G01N15/1434 , G02B21/32 , G01N15/1404
CPC分类号： G01N15/1409 , B01L3/502761 , G01N15/1434 , G02B21/32 , B01L2200/0663 , B01L2200/0668 , B01L2200/143 , B01L2300/1872 , B01L2400/0445 , B01L2400/0451 , G01N2015/1413
摘要： The invention concerns a method for spatially manipulating at least one particle in a fluid, wherein the particle or the particles is/are spatially manipulated in the fluid by hydrodynamic flows which are generated in the fluid by means of dynamic localized heating of the fluid. The method according to the invention is characterized in that at least one target spatial configuration of the particle(s) in the fluid is defined and that the following further steps are carried out: a) an actual spatial configuration of the particle(s) is captured, b) a specific dynamic localized heating event to be applied to the fluid is determined in dependence of at least one recent actual spatial configuration of the particle(s) and a target configuration of the particle(s), c) the specific dynamic localized heating as determined in step c) is applied at least once to the fluid and d) at least one or all of the steps a) to c) are repeated. The invention concerns furthermore an apparatus for spatially manipulating at least one particle in a fluid by means of hydrodynamic flows a computer program product and a computer-readable storage medium.

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